Restriction map for
pFN21AE4279
(Linear) (Six-base) MAPSORT of: KIEE4279.seq Check: 461 from: 1 to: 4146
KIEE4279.orf
With 182 enzymes: SgfI *
March 22, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2224 4146
Size: 2224 1922
AatII G_ACGT'C
Cuts at: 0 3230 4146
Size: 3230 916
Acc65I G'GTAC_C
Cuts at: 0 2042 4146
Size: 2042 2104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 3584 4146
Size: 691 2893 562
Fragments arranged by size:
2893 691 562
AfeI AGC'GCT
Cuts at: 0 526 4146
Size: 526 3620
AflII C'TTAA_G
Cuts at: 0 819 4146
Size: 819 3327
AflIII A'CryG_T
Cuts at: 0 1842 4146
Size: 1842 2304
AgeI A'CCGG_T
Cuts at: 0 1035 3699 4146
Size: 1035 2664 447
Fragments arranged by size:
2664 1035 447
AleI CACnn'nnGTG
Cuts at: 0 2605 2716 3086 4146
Size: 2605 111 370 1060
Fragments arranged by size:
2605 1060 370 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3263 4146
Size: 3263 883
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2999 3031 4146
Size: 2999 32 1115
Fragments arranged by size:
2999 1115 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1306 1437 4146
Size: 1306 131 2709
Fragments arranged by size:
2709 1306 131
ApaI G_GGCC'C
Cuts at: 0 1137 4146
Size: 1137 3009
ApaLI G'TGCA_C
Cuts at: 0 3160 4146
Size: 3160 986
ApoI r'AATT_y
Cuts at: 0 739 828 1899 1953 2443 2919 3028 3502
Size: 739 89 1071 54 490 476 109 474
Cuts at: 3502 4146
Size: 644
Fragments arranged by size:
1071 739 644 490 476 474 109 89
54
AscI GG'CGCG_CC
Cuts at: 0 80 4146
Size: 80 4066
AvaI C'yCGr_G
Cuts at: 0 3796 4146
Size: 3796 350
BanI G'GyrC_C
Cuts at: 0 2042 2814 2856 4141 4146
Size: 2042 772 42 1285 5
Fragments arranged by size:
2042 1285 772 42 5
BanII G_rGCy'C
Cuts at: 0 235 1137 1644 2898 3257 4146
Size: 235 902 507 1254 359 889
Fragments arranged by size:
1254 902 889 507 359 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 2137 3141 3572 4146
Size: 2137 1004 431 574
Fragments arranged by size:
2137 1004 574 431
BbvCI CC'TCA_GC
Cuts at: 0 2927 3682 4146
Size: 2927 755 464
Fragments arranged by size:
2927 755 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1626 1660 3675 3709 4146
Size: 1626 34 2015 34 437
Fragments arranged by size:
2015 1626 437 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2737 4146
Size: 2737 1409
BclI T'GATC_A
Cuts at: 0 1557 3720 4146
Size: 1557 2163 426
Fragments arranged by size:
2163 1557 426
BfrBI ATG'CAT
Cuts at: 0 1203 2157 4146
Size: 1203 954 1989
Fragments arranged by size:
1989 1203 954
BglI GCCn_nnn'nGGC
Cuts at: 0 97 196 1920 4146
Size: 97 99 1724 2226
Fragments arranged by size:
2226 1724 99 97
BglII A'GATC_T
Cuts at: 0 2688 2967 4146
Size: 2688 279 1179
Fragments arranged by size:
2688 1179 279
Bme1580I G_kGCm'C
Cuts at: 0 1137 2819 3164 3350 4146
Size: 1137 1682 345 186 796
Fragments arranged by size:
1682 1137 796 345 186
BmgBI CAC'GTC
Cuts at: 0 135 359 4146
Size: 135 224 3787
Fragments arranged by size:
3787 224 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 1131 3427 4146
Size: 1131 2296 719
Fragments arranged by size:
2296 1131 719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3247 4146
Size: 3247 899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1048 3105 3764 4146
Size: 716 332 2057 659 382
Fragments arranged by size:
2057 716 659 382 332
Bpu10I CC'TnA_GC
Cuts at: 0 2593 2927 3682 4146
Size: 2593 334 755 464
Fragments arranged by size:
2593 755 464 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2882 3283 4146
Size: 2882 401 863
Fragments arranged by size:
2882 863 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 3042 4146
Size: 3042 1104
BsaAI yAC'GTr
Cuts at: 0 3177 4146
Size: 3177 969
BsaBI GATnn'nnATC
Cuts at: 0 2972 4146
Size: 2972 1174
BsaHI Gr'CG_yC
Cuts at: 0 3227 3557 4146
Size: 3227 330 589
Fragments arranged by size:
3227 589 330
BsaWI w'CCGG_w
Cuts at: 0 1035 3383 3699 4146
Size: 1035 2348 316 447
Fragments arranged by size:
2348 1035 447 316
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 2026 2056 3802 3832 4146
Size: 614 30 1382 30 1746 30 314
Fragments arranged by size:
1746 1382 614 314 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1007 2293 3277 3670 4146
Size: 130 877 1286 984 393 476
Fragments arranged by size:
1286 984 877 476 393 130
BseYI C'CCAG_C
Cuts at: 0 75 554 4146
Size: 75 479 3592
Fragments arranged by size:
3592 479 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 3114 3157 4146
Size: 142 2972 43 989
Fragments arranged by size:
2972 989 142 43
BsiEI CG_ry'CG
Cuts at: 0 196 200 4080 4146
Size: 196 4 3880 66
Fragments arranged by size:
3880 196 66 4
BsiHKAI G_wGCw'C
Cuts at: 0 235 995 1644 1722 2898 3164 3257 3803
Size: 235 760 649 78 1176 266 93 546
Cuts at: 3803 4139 4146
Size: 336 7
Fragments arranged by size:
1176 760 649 546 336 266 235 93
78 7
BsmI GAATG_Cn'
Cuts at: 0 3587 4146
Size: 3587 559
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2589 3218 4146
Size: 2589 629 928
Fragments arranged by size:
2589 928 629
Bsp1286I G_dGCh'C
Cuts at: 0 235 995 1137 1644 1722 2819 2898 3164
Size: 235 760 142 507 78 1097 79 266
Cuts at: 3164 3257 3350 3803 4139 4146
Size: 93 93 453 336 7
Fragments arranged by size:
1097 760 507 453 336 266 235 142
93 93 79 78 7
BspEI T'CCGG_A
Cuts at: 0 3383 4146
Size: 3383 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1230 2224 4146
Size: 1230 994 1922
Fragments arranged by size:
1922 1230 994
BsrBI CCG'CTC
Cuts at: 0 3125 4146
Size: 3125 1021
BsrDI GCAATG_nn'
Cuts at: 0 1916 3950 4146
Size: 1916 2034 196
Fragments arranged by size:
2034 1916 196
BsrFI r'CCGG_y
Cuts at: 0 195 1035 1517 3699 4146
Size: 195 840 482 2182 447
Fragments arranged by size:
2182 840 482 447 195
BssHII G'CGCG_C
Cuts at: 0 80 4146
Size: 80 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2863 4146
Size: 2863 1283
BstBI TT'CG_AA
Cuts at: 0 209 4146
Size: 209 3937
BstEII G'GTnAC_C
Cuts at: 0 57 549 4146
Size: 57 492 3597
Fragments arranged by size:
3597 492 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3417 4146
Size: 3417 729
BstYI r'GATC_y
Cuts at: 0 2637 2688 2967 3619 4146
Size: 2637 51 279 652 527
Fragments arranged by size:
2637 652 527 279 51
BtgI C'CryG_G
Cuts at: 0 155 446 459 467 4146
Size: 155 291 13 8 3679
Fragments arranged by size:
3679 291 155 13 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 567 4146
Size: 567 3579
BtsI GCAGTG_nn'
Cuts at: 0 303 357 1164 1652 1733 4146
Size: 303 54 807 488 81 2413
Fragments arranged by size:
2413 807 488 303 81 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1320 1355 4146
Size: 1320 35 2791
Fragments arranged by size:
2791 1320 35
DraI TTT'AAA
Cuts at: 0 2004 2472 4146
Size: 2004 468 1674
Fragments arranged by size:
2004 1674 468
DrdI GACnn_nn'nnGTC
Cuts at: 0 2396 4146
Size: 2396 1750
EaeI y'GGCC_r
Cuts at: 0 107 193 197 1649 4146
Size: 107 86 4 1452 2497
Fragments arranged by size:
2497 1452 107 86 4
EagI C'GGCC_G
Cuts at: 0 193 197 4146
Size: 193 4 3949
Fragments arranged by size:
3949 193 4
EarI CTCTTCn'nnn_
Cuts at: 0 410 962 1779 4146
Size: 410 552 817 2367
Fragments arranged by size:
2367 817 552 410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 519 4146
Size: 519 3627
EcoICRI GAG'CTC
Cuts at: 0 233 1642 2896 3255 4146
Size: 233 1409 1254 359 891
Fragments arranged by size:
1409 1254 891 359 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 716 1048 3105 3584 3764 4146
Size: 691 25 332 2057 479 180 382
Fragments arranged by size:
2057 691 479 382 332 180 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3142 4146
Size: 3142 1004
EcoO109I rG'GnC_Cy
Cuts at: 0 1133 2310 2672 3303 4146
Size: 1133 1177 362 631 843
Fragments arranged by size:
1177 1133 843 631 362
EcoRI G'AATT_C
Cuts at: 0 739 2443 4146
Size: 739 1704 1703
Fragments arranged by size:
1704 1703 739
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 350 1283 2542 3324 4122 4146
Size: 350 933 1259 782 798 24
Fragments arranged by size:
1259 933 798 782 350 24
FseI GG_CCGG'CC
Cuts at: 0 199 4146
Size: 199 3947
FspI TGC'GCA
Cuts at: 0 3938 4146
Size: 3938 208
HaeII r_GCGC'y
Cuts at: 0 129 149 528 4146
Size: 129 20 379 3618
Fragments arranged by size:
3618 379 129 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 2622 2654 2885 2917 2979 3011
Size: 409 32 2181 32 231 32 62 32
Cuts at: 3011 4146
Size: 1135
Fragments arranged by size:
2181 1135 409 231 62 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2782 3964 4146
Size: 2782 1182 182
Fragments arranged by size:
2782 1182 182
KpnI G_GTAC'C
Cuts at: 0 2046 4146
Size: 2046 2100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 1127 1695 2482 4146
Size: 327 800 568 787 1664
Fragments arranged by size:
1664 800 787 568 327
MscI TGG'CCA
Cuts at: 0 1651 4146
Size: 1651 2495
MslI CAynn'nnrTG
Cuts at: 0 1081 1259 1763 1931 2605 2716 3086 3179
Size: 1081 178 504 168 674 111 370 93
Cuts at: 3179 4146
Size: 967
Fragments arranged by size:
1081 967 674 504 370 178 168 111
93
MspA1I CmG'CkG
Cuts at: 0 157 202 872 1466 4146
Size: 157 45 670 594 2680
Fragments arranged by size:
2680 670 594 157 45
NaeI GCC'GGC
Cuts at: 0 197 4146
Size: 197 3949
NcoI C'CATG_G
Cuts at: 0 459 4146
Size: 459 3687
NgoMIV G'CCGG_C
Cuts at: 0 195 4146
Size: 195 3951
NsiI A_TGCA'T
Cuts at: 0 1205 2159 4146
Size: 1205 954 1987
Fragments arranged by size:
1987 1205 954
NspI r_CATG'y
Cuts at: 0 1846 2157 2161 3690 4146
Size: 1846 311 4 1529 456
Fragments arranged by size:
1846 1529 456 311 4
PasI CC'CwG_GG
Cuts at: 0 367 618 1306 4146
Size: 367 251 688 2840
Fragments arranged by size:
2840 688 367 251
PciI A'CATG_T
Cuts at: 0 1842 4146
Size: 1842 2304
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 313 1306 3024 3104 4128 4146
Size: 135 178 993 1718 80 1024 18
Fragments arranged by size:
1718 1024 993 178 135 80 18
PfoI T'CCnGG_A
Cuts at: 0 1025 3203 4146
Size: 1025 2178 943
Fragments arranged by size:
2178 1025 943
PmlI CAC'GTG
Cuts at: 0 3177 4146
Size: 3177 969
PpuMI rG'GwC_Cy
Cuts at: 0 2310 2672 4146
Size: 2310 362 1474
Fragments arranged by size:
2310 1474 362
PshAI GACnn'nnGTC
Cuts at: 0 1497 2323 4146
Size: 1497 826 1823
Fragments arranged by size:
1823 1497 826
PsiI TTA'TAA
Cuts at: 0 380 4146
Size: 380 3766
PspOMI G'GGCC_C
Cuts at: 0 1133 4146
Size: 1133 3013
PstI C_TGCA'G
Cuts at: 0 541 2487 2550 4146
Size: 541 1946 63 1596
Fragments arranged by size:
1946 1596 541 63
PvuII CAG'CTG
Cuts at: 0 872 1466 4146
Size: 872 594 2680
Fragments arranged by size:
2680 872 594
SacI G_AGCT'C
Cuts at: 0 235 1644 2898 3257 4146
Size: 235 1409 1254 359 889
Fragments arranged by size:
1409 1254 889 359 235
SacII CC_GC'GG
Cuts at: 0 158 4146
Size: 158 3988
ScaI AGT'ACT
Cuts at: 0 3193 3736 4146
Size: 3193 543 410
Fragments arranged by size:
3193 543 410
SexAI A'CCwGG_T
Cuts at: 0 638 4146
Size: 638 3508
SfcI C'TryA_G
Cuts at: 0 537 2218 2374 2483 2546 3776 4146
Size: 537 1681 156 109 63 1230 370
Fragments arranged by size:
1681 1230 537 370 156 109 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 196 4146
Size: 196 3950
SgrAI Cr'CCGG_yG
Cuts at: 0 3699 4146
Size: 3699 447
SmlI C'TyrA_G
Cuts at: 0 819 2897 3298 4146
Size: 819 2078 401 848
Fragments arranged by size:
2078 848 819 401
SspI AAT'ATT
Cuts at: 0 1338 3861 4146
Size: 1338 2523 285
Fragments arranged by size:
2523 1338 285
StyI C'CwwG_G
Cuts at: 0 325 459 1836 3994 4146
Size: 325 134 1377 2158 152
Fragments arranged by size:
2158 1377 325 152 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 1174 1618 2846 4146
Size: 568 606 444 1228 1300
Fragments arranged by size:
1300 1228 606 568 444
TatI w'GTAC_w
Cuts at: 0 765 3191 3734 4146
Size: 765 2426 543 412
Fragments arranged by size:
2426 765 543 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 4146
Size: 614 32 3500
Fragments arranged by size:
3500 614 32
Tth111I GACn'n_nGTC
Cuts at: 0 2845 4146
Size: 2845 1301
XbaI T'CTAG_A
Cuts at: 0 1501 1857 4146
Size: 1501 356 2289
Fragments arranged by size:
2289 1501 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 310 732 4146
Size: 310 422 3414
Fragments arranged by size:
3414 422 310
XmnI GAAnn'nnTTC
Cuts at: 0 828 3148 3569 4146
Size: 828 2320 421 577
Fragments arranged by size:
2320 828 577 421
ZraI GAC'GTC
Cuts at: 0 3228 4146
Size: 3228 918
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SacII ScaI
SexAI SfcI SfiI SgrAI SmlI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AhdI AseI AsiSI AvrII BaeI BamHI
BbeI BlpI BmtI BsiWI BspHI BsrGI BssSI BstZ17I
Bsu36I ClaI DraIII EcoRV FspAI HincII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI PacI
PmeI PpiI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XhoI XmaI