Restriction map for
pFN21AE4259
(Linear) (Six-base) MAPSORT of: KIEE4259.seq Check: 868 from: 1 to: 3114
KIEE4259.orf
With 182 enzymes: SgfI *
March 22, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 154 3114
Size: 154 2960
AccI GT'mk_AC
Cuts at: 0 637 3114
Size: 637 2477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 887 898 1045 1351 1377 2696 2831
Size: 394 493 11 147 306 26 1319 135
Cuts at: 2831 3114
Size: 283
Fragments arranged by size:
1319 493 394 306 283 147 135 26
11
AflIII A'CryG_T
Cuts at: 0 1364 1987 3114
Size: 1364 623 1127
Fragments arranged by size:
1364 1127 623
AhdI GACnn_n'nnGTC
Cuts at: 0 2945 3114
Size: 2945 169
AleI CACnn'nnGTG
Cuts at: 0 2010 3114
Size: 2010 1104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1443 2646 3114
Size: 1443 1203 468
Fragments arranged by size:
1443 1203 468
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1087 1819 2937 3114
Size: 672 415 732 1118 177
Fragments arranged by size:
1118 732 672 415 177
ApoI r'AATT_y
Cuts at: 0 99 440 689 859 1829 1948 3052 3114
Size: 99 341 249 170 970 119 1104 62
Fragments arranged by size:
1104 970 341 249 170 119 99 62
AseI AT'TA_AT
Cuts at: 0 986 3114
Size: 986 2128
AvaI C'yCGr_G
Cuts at: 0 956 3114
Size: 956 2158
BanI G'GyrC_C
Cuts at: 0 34 3114
Size: 34 3080
BanII G_rGCy'C
Cuts at: 0 314 1296 3114
Size: 314 982 1818
Fragments arranged by size:
1818 982 314
BbsI GAAGACnn'nnnn_
Cuts at: 0 1115 3114
Size: 1115 1999
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 440 3114
Size: 406 34 2674
Fragments arranged by size:
2674 406 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3040 3114
Size: 3040 74
BclI T'GATC_A
Cuts at: 0 579 1470 1571 2997 3114
Size: 579 891 101 1426 117
Fragments arranged by size:
1426 891 579 117 101
BfrBI ATG'CAT
Cuts at: 0 338 3114
Size: 338 2776
BglII A'GATC_T
Cuts at: 0 614 1340 1961 3114
Size: 614 726 621 1153
Fragments arranged by size:
1153 726 621 614
BlpI GC'TnA_GC
Cuts at: 0 2759 3114
Size: 2759 355
Bme1580I G_kGCm'C
Cuts at: 0 37 3114
Size: 37 3077
BmrI ACTGGGnnnn_n'
Cuts at: 0 2710 3114
Size: 2710 404
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1983 3114
Size: 1983 1131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 3047 3114
Size: 103 2944 67
Fragments arranged by size:
2944 103 67
Bpu10I CC'TnA_GC
Cuts at: 0 308 3114
Size: 308 2806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1915 3114
Size: 1915 1199
BsaBI GATnn'nnATC
Cuts at: 0 2873 3114
Size: 2873 241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 944 1972 3114
Size: 508 436 1028 1142
Fragments arranged by size:
1142 1028 508 436
BseYI C'CCAG_C
Cuts at: 0 45 1430 3096 3114
Size: 45 1385 1666 18
Fragments arranged by size:
1666 1385 45 18
BsiHKAI G_wGCw'C
Cuts at: 0 314 2529 3063 3114
Size: 314 2215 534 51
Fragments arranged by size:
2215 534 314 51
BsmI GAATG_Cn'
Cuts at: 0 338 2270 3114
Size: 338 1932 844
Fragments arranged by size:
1932 844 338
Bsp1286I G_dGCh'C
Cuts at: 0 37 314 1296 2529 3063 3114
Size: 37 277 982 1233 534 51
Fragments arranged by size:
1233 982 534 277 51 37
BspHI T'CATG_A
Cuts at: 0 2493 3114
Size: 2493 621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 3114
Size: 154 2960
BsrBI CCG'CTC
Cuts at: 0 498 3114
Size: 498 2616
BsrDI GCAATG_nn'
Cuts at: 0 524 843 1553 1760 3114
Size: 524 319 710 207 1354
Fragments arranged by size:
1354 710 524 319 207
BsrFI r'CCGG_y
Cuts at: 0 2586 3114
Size: 2586 528
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 1819 3114
Size: 531 1288 1295
Fragments arranged by size:
1295 1288 531
BstBI TT'CG_AA
Cuts at: 0 2334 3114
Size: 2334 780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 374 2012 3114
Size: 374 1638 1102
Fragments arranged by size:
1638 1102 374
BstYI r'GATC_y
Cuts at: 0 614 1340 1961 3114
Size: 614 726 621 1153
Fragments arranged by size:
1153 726 621 614
BstZ17I GTA'TAC
Cuts at: 0 638 3114
Size: 638 2476
BtgI C'CryG_G
Cuts at: 0 38 62 766 3114
Size: 38 24 704 2348
Fragments arranged by size:
2348 704 38 24
BtsI GCAGTG_nn'
Cuts at: 0 112 1169 1404 2248 3114
Size: 112 1057 235 844 866
Fragments arranged by size:
1057 866 844 235 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2235 2270 2826 2861 3114
Size: 2235 35 556 35 253
Fragments arranged by size:
2235 556 253 35 35
DraI TTT'AAA
Cuts at: 0 695 2561 2886 3114
Size: 695 1866 325 228
Fragments arranged by size:
1866 695 325 228
EarI CTCTTCn'nnn_
Cuts at: 0 1396 1640 2807 3000 3114
Size: 1396 244 1167 193 114
Fragments arranged by size:
1396 1167 244 193 114
EcoICRI GAG'CTC
Cuts at: 0 312 3114
Size: 312 2802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 394 887 898 1045 1351 1377 2696
Size: 103 291 493 11 147 306 26 1319
Cuts at: 2696 2831 3047 3114
Size: 135 216 67
Fragments arranged by size:
1319 493 306 291 216 147 135 103
67 26 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 3114
Size: 55 3059
EcoO109I rG'GnC_Cy
Cuts at: 0 24 3114
Size: 24 3090
EcoRI G'AATT_C
Cuts at: 0 440 1829 1948 3114
Size: 440 1389 119 1166
Fragments arranged by size:
1389 1166 440 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 807 3114
Size: 807 2307
HaeII r_GCGC'y
Cuts at: 0 72 3114
Size: 72 3042
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1563 1595 2931 2963 3114
Size: 1563 32 1336 32 151
Fragments arranged by size:
1563 1336 151 32 32
HincII GTy'rAC
Cuts at: 0 1993 2475 3114
Size: 1993 482 639
Fragments arranged by size:
1993 639 482
MfeI C'AATT_G
Cuts at: 0 1841 2357 3114
Size: 1841 516 757
Fragments arranged by size:
1841 757 516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 3114
Size: 113 3001
MslI CAynn'nnrTG
Cuts at: 0 1071 2010 2096 2648 3114
Size: 1071 939 86 552 466
Fragments arranged by size:
1071 939 552 466 86
MspA1I CmG'CkG
Cuts at: 0 64 141 374 3114
Size: 64 77 233 2740
Fragments arranged by size:
2740 233 77 64
NaeI GCC'GGC
Cuts at: 0 2588 3114
Size: 2588 526
NcoI C'CATG_G
Cuts at: 0 38 766 3114
Size: 38 728 2348
Fragments arranged by size:
2348 728 38
NdeI CA'TA_TG
Cuts at: 0 1301 2625 3114
Size: 1301 1324 489
Fragments arranged by size:
1324 1301 489
NgoMIV G'CCGG_C
Cuts at: 0 2586 3114
Size: 2586 528
NsiI A_TGCA'T
Cuts at: 0 340 3114
Size: 340 2774
NspI r_CATG'y
Cuts at: 0 338 1368 1942 1991 3114
Size: 338 1030 574 49 1123
Fragments arranged by size:
1123 1030 574 338 49
PciI A'CATG_T
Cuts at: 0 1364 1987 3114
Size: 1364 623 1127
Fragments arranged by size:
1364 1127 623
PsiI TTA'TAA
Cuts at: 0 1648 2375 3114
Size: 1648 727 739
Fragments arranged by size:
1648 739 727
PspXI vC'TCGA_Gb
Cuts at: 0 956 3114
Size: 956 2158
PstI C_TGCA'G
Cuts at: 0 2243 3114
Size: 2243 871
PvuII CAG'CTG
Cuts at: 0 141 3114
Size: 141 2973
SacI G_AGCT'C
Cuts at: 0 314 3114
Size: 314 2800
SacII CC_GC'GG
Cuts at: 0 65 3114
Size: 65 3049
SapI GCTCTTCn'nnn_
Cuts at: 0 1640 3000 3114
Size: 1640 1360 114
Fragments arranged by size:
1640 1360 114
SfcI C'TryA_G
Cuts at: 0 1200 2239 3114
Size: 1200 1039 875
Fragments arranged by size:
1200 1039 875
SmlI C'TyrA_G
Cuts at: 0 956 1894 3114
Size: 956 938 1220
Fragments arranged by size:
1220 956 938
SspI AAT'ATT
Cuts at: 0 282 2743 3114
Size: 282 2461 371
Fragments arranged by size:
2461 371 282
StyI C'CwwG_G
Cuts at: 0 38 766 845 3114
Size: 38 728 79 2269
Fragments arranged by size:
2269 728 79 38
TatI w'GTAC_w
Cuts at: 0 929 3114
Size: 929 2185
XhoI C'TCGA_G
Cuts at: 0 956 3114
Size: 956 2158
XmnI GAAnn'nnTTC
Cuts at: 0 731 872 1325 2042 2408 3114
Size: 731 141 453 717 366 706
Fragments arranged by size:
731 717 706 453 366 141
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AseI AvaI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PciI PsiI PspXI PstI PvuII SacI
SacII SapI SfcI SmlI SspI StyI TatI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BglI BmgBI BmtI BsaI BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI
BstEII Bsu36I BtgZI ClaI DraIII DrdI EaeI EagI
EciI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MscI NarI NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI ZraI