Restriction map for
pFN21AE4255
(Linear) (Six-base) MAPSORT of: KIEE4255.seq Check: 2513 from: 1 to: 3066
KIEE4255.orf
With 182 enzymes: SgfI *
July 3, 2012 18:51 ..
AccI GT'mk_AC
Cuts at: 0 1731 3066
Size: 1731 1335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 718 1040 2096 2699 3066
Size: 329 389 322 1056 603 367
Fragments arranged by size:
1056 603 389 367 329 322
AflIII A'CryG_T
Cuts at: 0 1105 1375 3066
Size: 1105 270 1691
Fragments arranged by size:
1691 1105 270
AleI CACnn'nnGTG
Cuts at: 0 745 3066
Size: 745 2321
ApaLI G'TGCA_C
Cuts at: 0 692 907 3066
Size: 692 215 2159
Fragments arranged by size:
2159 692 215
ApoI r'AATT_y
Cuts at: 0 331 1993 2110 2123 2620 2739 2833 3066
Size: 331 1662 117 13 497 119 94 233
Fragments arranged by size:
1662 497 331 233 119 117 94 13
AseI AT'TA_AT
Cuts at: 0 2117 3066
Size: 2117 949
AvaI C'yCGr_G
Cuts at: 0 61 1114 1381 3066
Size: 61 1053 267 1685
Fragments arranged by size:
1685 1053 267 61
BamHI G'GATC_C
Cuts at: 0 539 3066
Size: 539 2527
BanI G'GyrC_C
Cuts at: 0 16 3066
Size: 16 3050
BanII G_rGCy'C
Cuts at: 0 573 1404 3066
Size: 573 831 1662
Fragments arranged by size:
1662 831 573
BbeI G_GCGC'C
Cuts at: 0 20 3066
Size: 20 3046
BbsI GAAGACnn'nnnn_
Cuts at: 0 279 1028 1806 3066
Size: 279 749 778 1260
Fragments arranged by size:
1260 778 749 279
BciVI GTATCCnnnnn_n'
Cuts at: 0 799 1086 2222 2752 3066
Size: 799 287 1136 530 314
Fragments arranged by size:
1136 799 530 314 287
BfrBI ATG'CAT
Cuts at: 0 1473 1965 3066
Size: 1473 492 1101
Fragments arranged by size:
1473 1101 492
BglI GCCn_nnn'nGGC
Cuts at: 0 568 3066
Size: 568 2498
BlpI GC'TnA_GC
Cuts at: 0 68 3066
Size: 68 2998
Bme1580I G_kGCm'C
Cuts at: 0 696 911 3066
Size: 696 215 2155
Fragments arranged by size:
2155 696 215
BmgBI CAC'GTC
Cuts at: 0 2857 3066
Size: 2857 209
BmrI ACTGGGnnnn_n'
Cuts at: 0 655 1838 2499 3066
Size: 655 1183 661 567
Fragments arranged by size:
1183 661 655 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 516 2482 3066
Size: 117 399 1966 584
Fragments arranged by size:
1966 584 399 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 553 1301 2159 3066
Size: 9 544 748 858 907
Fragments arranged by size:
907 858 748 544 9
Bpu10I CC'TnA_GC
Cuts at: 0 551 3066
Size: 551 2515
BsaAI yAC'GTr
Cuts at: 0 1108 1376 3066
Size: 1108 268 1690
Fragments arranged by size:
1690 1108 268
BsaHI Gr'CG_yC
Cuts at: 0 17 3066
Size: 17 3049
BsaWI w'CCGG_w
Cuts at: 0 972 3066
Size: 972 2094
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 1095 1125 3066
Size: 400 30 665 30 1941
Fragments arranged by size:
1941 665 400 30 30
BseYI C'CCAG_C
Cuts at: 0 162 318 477 611 801 3066
Size: 162 156 159 134 190 2265
Fragments arranged by size:
2265 190 162 159 156 134
BsiHKAI G_wGCw'C
Cuts at: 0 257 696 911 1113 1404 3066
Size: 257 439 215 202 291 1662
Fragments arranged by size:
1662 439 291 257 215 202
BsmI GAATG_Cn'
Cuts at: 0 2291 3066
Size: 2291 775
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2601 3066
Size: 2601 465
Bsp1286I G_dGCh'C
Cuts at: 0 257 573 696 911 1113 1404 3066
Size: 257 316 123 215 202 291 1662
Fragments arranged by size:
1662 316 291 257 215 202 123
BspEI T'CCGG_A
Cuts at: 0 972 3066
Size: 972 2094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1839 3066
Size: 1839 1227
BssHII G'CGCG_C
Cuts at: 0 176 3066
Size: 176 2890
BssSI C'ACGA_G
Cuts at: 0 135 235 3066
Size: 135 100 2831
Fragments arranged by size:
2831 135 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 742 3066
Size: 742 2324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1302 2538 3066
Size: 1302 1236 528
Fragments arranged by size:
1302 1236 528
BstYI r'GATC_y
Cuts at: 0 539 2525 3066
Size: 539 1986 541
Fragments arranged by size:
1986 541 539
BtgI C'CryG_G
Cuts at: 0 468 1154 3066
Size: 468 686 1912
Fragments arranged by size:
1912 686 468
BtsI GCAGTG_nn'
Cuts at: 0 740 1181 3066
Size: 740 441 1885
Fragments arranged by size:
1885 740 441
DraI TTT'AAA
Cuts at: 0 1265 2315 2390 3066
Size: 1265 1050 75 676
Fragments arranged by size:
1265 1050 676 75
EaeI y'GGCC_r
Cuts at: 0 83 3066
Size: 83 2983
EarI CTCTTCn'nnn_
Cuts at: 0 820 1409 2171 2410 3066
Size: 820 589 762 239 656
Fragments arranged by size:
820 762 656 589 239
EcoICRI GAG'CTC
Cuts at: 0 1402 3066
Size: 1402 1664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 329 553 718 1040 1301 2096 2159
Size: 9 320 224 165 322 261 795 63
Cuts at: 2159 2699 3066
Size: 540 367
Fragments arranged by size:
795 540 367 322 320 261 224 165
63 9
EcoO109I rG'GnC_Cy
Cuts at: 0 129 527 978 3066
Size: 129 398 451 2088
Fragments arranged by size:
2088 451 398 129
EcoRI G'AATT_C
Cuts at: 0 331 2739 3066
Size: 331 2408 327
Fragments arranged by size:
2408 331 327
HaeII r_GCGC'y
Cuts at: 0 20 42 99 3066
Size: 20 22 57 2967
Fragments arranged by size:
2967 57 22 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 775 807 1071 1103 2261 2293 3026 3058
Size: 775 32 264 32 1158 32 733 32
Cuts at: 3058 3066
Size: 8
Fragments arranged by size:
1158 775 733 264 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 1340 1732 2508 3066
Size: 1340 392 776 558
Fragments arranged by size:
1340 776 558 392
HindIII A'AGCT_T
Cuts at: 0 1430 3066
Size: 1430 1636
HpaI GTT'AAC
Cuts at: 0 1340 3066
Size: 1340 1726
KasI G'GCGC_C
Cuts at: 0 16 3066
Size: 16 3050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1793 3066
Size: 1793 1273
MslI CAynn'nnrTG
Cuts at: 0 745 1300 1670 1960 2536 3066
Size: 745 555 370 290 576 530
Fragments arranged by size:
745 576 555 530 370 290
MspA1I CmG'CkG
Cuts at: 0 89 162 318 477 636 725 801 1599
Size: 89 73 156 159 159 89 76 798
Cuts at: 1599 1635 3066
Size: 36 1431
Fragments arranged by size:
1431 798 159 159 156 89 89 76
73 36
NarI GG'CG_CC
Cuts at: 0 17 3066
Size: 17 3049
NcoI C'CATG_G
Cuts at: 0 468 1154 3066
Size: 468 686 1912
Fragments arranged by size:
1912 686 468
NdeI CA'TA_TG
Cuts at: 0 903 1065 3066
Size: 903 162 2001
Fragments arranged by size:
2001 903 162
NsiI A_TGCA'T
Cuts at: 0 1475 1967 3066
Size: 1475 492 1099
Fragments arranged by size:
1475 1099 492
NspI r_CATG'y
Cuts at: 0 1959 2235 2872 3066
Size: 1959 276 637 194
Fragments arranged by size:
1959 637 276 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1155 2197 3066
Size: 420 735 1042 869
Fragments arranged by size:
1042 869 735 420
PmlI CAC'GTG
Cuts at: 0 1108 3066
Size: 1108 1958
PpuMI rG'GwC_Cy
Cuts at: 0 129 527 3066
Size: 129 398 2539
Fragments arranged by size:
2539 398 129
PstI C_TGCA'G
Cuts at: 0 38 80 1836 3066
Size: 38 42 1756 1230
Fragments arranged by size:
1756 1230 42 38
PvuII CAG'CTG
Cuts at: 0 162 318 477 636 725 801 1635 3066
Size: 162 156 159 159 89 76 834 1431
Fragments arranged by size:
1431 834 162 159 159 156 89 76
SacI G_AGCT'C
Cuts at: 0 1404 3066
Size: 1404 1662
SalI G'TCGA_C
Cuts at: 0 1730 3066
Size: 1730 1336
SapI GCTCTTCn'nnn_
Cuts at: 0 1409 3066
Size: 1409 1657
ScaI AGT'ACT
Cuts at: 0 1191 3066
Size: 1191 1875
SexAI A'CCwGG_T
Cuts at: 0 1496 3066
Size: 1496 1570
SfcI C'TryA_G
Cuts at: 0 34 76 1832 3066
Size: 34 42 1756 1234
Fragments arranged by size:
1756 1234 42 34
SfoI GGC'GCC
Cuts at: 0 18 3066
Size: 18 3048
SmaI CCC'GGG
Cuts at: 0 63 1116 3066
Size: 63 1053 1950
Fragments arranged by size:
1950 1053 63
SmlI C'TyrA_G
Cuts at: 0 1381 3066
Size: 1381 1685
SpeI A'CTAG_T
Cuts at: 0 1096 3066
Size: 1096 1970
SphI G_CATG'C
Cuts at: 0 2872 3066
Size: 2872 194
SspI AAT'ATT
Cuts at: 0 2961 3066
Size: 2961 105
StyI C'CwwG_G
Cuts at: 0 468 1154 2816 3066
Size: 468 686 1662 250
Fragments arranged by size:
1662 686 468 250
SwaI ATTT'AAAT
Cuts at: 0 2315 3066
Size: 2315 751
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2022 2951 3063 3066
Size: 2022 929 112 3
Fragments arranged by size:
2022 929 112 3
TatI w'GTAC_w
Cuts at: 0 2 1189 3066
Size: 2 1187 1877
Fragments arranged by size:
1877 1187 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1095 1127 3066
Size: 400 32 663 32 1939
Fragments arranged by size:
1939 663 400 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2033 3066
Size: 2033 1033
XbaI T'CTAG_A
Cuts at: 0 2433 3066
Size: 2433 633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 921 3066
Size: 921 2145
XhoI C'TCGA_G
Cuts at: 0 1381 3066
Size: 1381 1685
XmaI C'CCGG_G
Cuts at: 0 61 1114 3066
Size: 61 1053 1952
Fragments arranged by size:
1952 1053 61
XmnI GAAnn'nnTTC
Cuts at: 0 203 493 769 1973 2800 2833 3008 3066
Size: 203 290 276 1204 827 33 175 58
Fragments arranged by size:
1204 827 290 276 203 175 58 33
Enzymes that do cut:
AccI AcuI AflIII AleI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BssHII BssSI BstAPI BstXI BstYI BtgI
BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI
MspA1I NarI NcoI NdeI NsiI NspI PflMI PmlI
PpuMI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SfoI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI AscI AsiSI AvrII BaeI
BbvCI BcgI BclI BglII BmtI BpuEI BsaI BsaBI
BseRI BsgI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI
BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI
FspI FspAI KpnI MfeI MluI MscI NaeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SgfI SgrAI SnaBI SrfI
StuI ZraI