(Linear) (Six-base) MAPSORT of: KIEE4254.seq Check: 4070 from: 1 to: 3027 KIEE4254.orf With 182 enzymes: SgfI * March 22, 2012 17:38 .. AatII G_ACGT'C Cuts at: 0 1558 1721 2201 3027 Size: 1558 163 480 826 Fragments arranged by size: 1558 826 480 163 AccI GT'mk_AC Cuts at: 0 1289 3027 Size: 1289 1738 AclI AA'CG_TT Cuts at: 0 2767 3027 Size: 2767 260 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 898 1707 3027 Size: 254 644 809 1320 Fragments arranged by size: 1320 809 644 254 AflII C'TTAA_G Cuts at: 0 297 3027 Size: 297 2730 AflIII A'CryG_T Cuts at: 0 548 674 1506 3027 Size: 548 126 832 1521 Fragments arranged by size: 1521 832 548 126 AleI CACnn'nnGTG Cuts at: 0 217 3027 Size: 217 2810 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2909 3027 Size: 2909 118 AlwNI CAG_nnn'CTG Cuts at: 0 91 1062 1669 1758 1839 2730 2817 3027 Size: 91 971 607 89 81 891 87 210 Fragments arranged by size: 971 891 607 210 91 89 87 81 ApaI G_GGCC'C Cuts at: 0 2571 3027 Size: 2571 456 AvaI C'yCGr_G Cuts at: 0 1990 2314 2958 3027 Size: 1990 324 644 69 Fragments arranged by size: 1990 644 324 69 BamHI G'GATC_C Cuts at: 0 2759 3027 Size: 2759 268 BanI G'GyrC_C Cuts at: 0 2064 2182 2704 3027 Size: 2064 118 522 323 Fragments arranged by size: 2064 522 323 118 BanII G_rGCy'C Cuts at: 0 7 1570 2255 2571 2719 2959 3027 Size: 7 1563 685 316 148 240 68 Fragments arranged by size: 1563 685 316 240 148 68 7 BbeI G_GCGC'C Cuts at: 0 2186 3027 Size: 2186 841 BbsI GAAGACnn'nnnn_ Cuts at: 0 1288 1460 1713 1721 2122 3027 Size: 1288 172 253 8 401 905 Fragments arranged by size: 1288 905 401 253 172 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1570 1604 2203 2237 3027 Size: 1570 34 599 34 790 Fragments arranged by size: 1570 790 599 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 581 3027 Size: 581 2446 BglI GCCn_nnn'nGGC Cuts at: 0 200 2016 2856 3027 Size: 200 1816 840 171 Fragments arranged by size: 1816 840 200 171 BglII A'GATC_T Cuts at: 0 1116 3027 Size: 1116 1911 BlpI GC'TnA_GC Cuts at: 0 2302 2365 3027 Size: 2302 63 662 Fragments arranged by size: 2302 662 63 Bme1580I G_kGCm'C Cuts at: 0 1794 2069 2571 2637 2707 3027 Size: 1794 275 502 66 70 320 Fragments arranged by size: 1794 502 320 275 70 66 BmgBI CAC'GTC Cuts at: 0 864 2952 3027 Size: 864 2088 75 Fragments arranged by size: 2088 864 75 BmrI ACTGGGnnnn_n' Cuts at: 0 920 1768 2042 3027 Size: 920 848 274 985 Fragments arranged by size: 985 920 848 274 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 952 1995 3027 Size: 952 1043 1032 Fragments arranged by size: 1043 1032 952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1935 2155 2278 2558 2852 2893 3027 Size: 829 1106 220 123 280 294 41 134 Fragments arranged by size: 1106 829 294 280 220 134 123 41 Bpu10I CC'TnA_GC Cuts at: 0 1571 3001 3027 Size: 1571 1430 26 Fragments arranged by size: 1571 1430 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 2769 2849 3027 Size: 922 1847 80 178 Fragments arranged by size: 1847 922 178 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 22 903 1512 2547 2651 2797 2828 2906 Size: 22 881 609 1035 104 146 31 78 Cuts at: 2906 3010 3027 Size: 104 17 Fragments arranged by size: 1035 881 609 146 104 104 78 31 22 17 BsaAI yAC'GTr Cuts at: 0 675 3027 Size: 675 2352 BsaBI GATnn'nnATC Cuts at: 0 592 3027 Size: 592 2435 BsaHI Gr'CG_yC Cuts at: 0 1555 1718 2183 2198 2339 3027 Size: 1555 163 465 15 141 688 Fragments arranged by size: 1555 688 465 163 141 15 BsaWI w'CCGG_w Cuts at: 0 1042 1223 3027 Size: 1042 181 1804 Fragments arranged by size: 1804 1042 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 422 452 1024 1054 3027 Size: 422 30 572 30 1973 Fragments arranged by size: 1973 572 422 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1009 1875 2074 2888 3027 Size: 1009 866 199 814 139 Fragments arranged by size: 1009 866 814 199 139 BseYI C'CCAG_C Cuts at: 0 490 1674 1945 1956 2127 2730 2739 3027 Size: 490 1184 271 11 171 603 9 288 Fragments arranged by size: 1184 603 490 288 271 171 11 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 388 1394 3027 Size: 118 270 1006 1633 Fragments arranged by size: 1633 1006 270 118 BsiHKAI G_wGCw'C Cuts at: 0 224 723 3027 Size: 224 499 2304 Fragments arranged by size: 2304 499 224 BsmI GAATG_Cn' Cuts at: 0 453 3027 Size: 453 2574 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2205 3027 Size: 2205 822 Bsp1286I G_dGCh'C Cuts at: 0 7 224 723 1570 1794 2069 2255 2571 Size: 7 217 499 847 224 275 186 316 Cuts at: 2571 2637 2707 2719 2959 3027 Size: 66 70 12 240 68 Fragments arranged by size: 847 499 316 275 240 224 217 186 70 68 66 12 7 BspEI T'CCGG_A Cuts at: 0 1042 1223 3027 Size: 1042 181 1804 Fragments arranged by size: 1804 1042 181 BsrBI CCG'CTC Cuts at: 0 1040 2208 2301 2364 2783 3027 Size: 1040 1168 93 63 419 244 Fragments arranged by size: 1168 1040 419 244 93 63 BsrDI GCAATG_nn' Cuts at: 0 1358 3027 Size: 1358 1669 BsrFI r'CCGG_y Cuts at: 0 1457 2179 2589 3027 Size: 1457 722 410 438 Fragments arranged by size: 1457 722 438 410 BsrGI T'GTAC_A Cuts at: 0 677 3027 Size: 677 2350 BssSI C'ACGA_G Cuts at: 0 2328 2712 3027 Size: 2328 384 315 Fragments arranged by size: 2328 384 315 BstAPI GCAn_nnn'nTGC Cuts at: 0 1062 1650 3027 Size: 1062 588 1377 Fragments arranged by size: 1377 1062 588 BstYI r'GATC_y Cuts at: 0 87 1116 1842 2759 3027 Size: 87 1029 726 917 268 Fragments arranged by size: 1029 917 726 268 87 Bsu36I CC'TnA_GG Cuts at: 0 92 1804 2824 3027 Size: 92 1712 1020 203 Fragments arranged by size: 1712 1020 203 92 BtgI C'CryG_G Cuts at: 0 633 1866 2699 3027 Size: 633 1233 833 328 Fragments arranged by size: 1233 833 633 328 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 661 1518 3027 Size: 661 857 1509 Fragments arranged by size: 1509 857 661 BtsI GCAGTG_nn' Cuts at: 0 1194 1544 1747 2340 2881 3016 3027 Size: 1194 350 203 593 541 135 11 Fragments arranged by size: 1194 593 541 350 203 135 11 DraIII CAC_nnn'GTG Cuts at: 0 2194 3027 Size: 2194 833 DrdI GACnn_nn'nnGTC Cuts at: 0 1413 3027 Size: 1413 1614 EaeI y'GGCC_r Cuts at: 0 201 923 1274 1462 1937 2162 2688 3027 Size: 201 722 351 188 475 225 526 339 Fragments arranged by size: 722 526 475 351 339 225 201 188 EarI CTCTTCn'nnn_ Cuts at: 0 274 876 1093 1151 1385 3027 Size: 274 602 217 58 234 1642 Fragments arranged by size: 1642 602 274 234 217 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2652 3027 Size: 2652 375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 829 898 1707 1935 2155 2278 2558 Size: 254 575 69 809 228 220 123 280 Cuts at: 2558 2852 2893 3027 Size: 294 41 134 Fragments arranged by size: 809 575 294 280 254 228 220 134 123 69 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 63 425 1924 3027 Size: 63 362 1499 1103 Fragments arranged by size: 1499 1103 362 63 EcoO109I rG'GnC_Cy Cuts at: 0 1437 1666 1782 1820 2018 2278 2567 2568 Size: 1437 229 116 38 198 260 289 1 Cuts at: 2568 2734 2858 3027 Size: 166 124 169 Fragments arranged by size: 1437 289 260 229 198 169 166 124 116 38 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 303 3027 Size: 303 2724 HaeII r_GCGC'y Cuts at: 0 2186 3027 Size: 2186 841 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 422 454 853 885 1827 1859 2946 2978 Size: 422 32 399 32 942 32 1087 32 Cuts at: 2978 3027 Size: 49 Fragments arranged by size: 1087 942 422 399 49 32 32 32 32 HincII GTy'rAC Cuts at: 0 543 3027 Size: 543 2484 HindIII A'AGCT_T Cuts at: 0 1888 3027 Size: 1888 1139 HpaI GTT'AAC Cuts at: 0 543 3027 Size: 543 2484 KasI G'GCGC_C Cuts at: 0 2182 3027 Size: 2182 845 MscI TGG'CCA Cuts at: 0 203 925 1939 2690 3027 Size: 203 722 1014 751 337 Fragments arranged by size: 1014 751 722 337 203 MslI CAynn'nnrTG Cuts at: 0 217 685 1537 3027 Size: 217 468 852 1490 Fragments arranged by size: 1490 852 468 217 MspA1I CmG'CkG Cuts at: 0 1080 1398 1868 1960 2730 3027 Size: 1080 318 470 92 770 297 Fragments arranged by size: 1080 770 470 318 297 92 NaeI GCC'GGC Cuts at: 0 2181 2591 3027 Size: 2181 410 436 Fragments arranged by size: 2181 436 410 NarI GG'CG_CC Cuts at: 0 2183 3027 Size: 2183 844 NcoI C'CATG_G Cuts at: 0 2699 3027 Size: 2699 328 NgoMIV G'CCGG_C Cuts at: 0 2179 2589 3027 Size: 2179 410 438 Fragments arranged by size: 2179 438 410 NspI r_CATG'y Cuts at: 0 552 1510 3027 Size: 552 958 1517 Fragments arranged by size: 1517 958 552 PasI CC'CwG_GG Cuts at: 0 2583 3027 Size: 2583 444 PciI A'CATG_T Cuts at: 0 548 1506 3027 Size: 548 958 1521 Fragments arranged by size: 1521 958 548 PflMI CCAn_nnn'nTGG Cuts at: 0 665 3027 Size: 665 2362 PfoI T'CCnGG_A Cuts at: 0 7 110 2354 3027 Size: 7 103 2244 673 Fragments arranged by size: 2244 673 103 7 PmlI CAC'GTG Cuts at: 0 675 3027 Size: 675 2352 PpuMI rG'GwC_Cy Cuts at: 0 1437 1782 1820 2734 3027 Size: 1437 345 38 914 293 Fragments arranged by size: 1437 914 345 293 38 PsiI TTA'TAA Cuts at: 0 329 3027 Size: 329 2698 PspOMI G'GGCC_C Cuts at: 0 2567 3027 Size: 2567 460 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 572 604 3027 Size: 572 32 2423 Fragments arranged by size: 2423 572 32 PstI C_TGCA'G Cuts at: 0 1067 1085 1754 2216 2325 2460 2888 3027 Size: 1067 18 669 462 109 135 428 139 Fragments arranged by size: 1067 669 462 428 139 135 109 18 PvuII CAG'CTG Cuts at: 0 1080 1398 1960 3027 Size: 1080 318 562 1067 Fragments arranged by size: 1080 1067 562 318 SacII CC_GC'GG Cuts at: 0 1869 3027 Size: 1869 1158 SanDI GG'GwC_CC Cuts at: 0 2734 3027 Size: 2734 293 SapI GCTCTTCn'nnn_ Cuts at: 0 274 1093 1385 3027 Size: 274 819 292 1642 Fragments arranged by size: 1642 819 292 274 SbfI CC_TGCA'GG Cuts at: 0 2460 3027 Size: 2460 567 SfcI C'TryA_G Cuts at: 0 120 139 1063 1081 1750 2212 2321 2456 Size: 120 19 924 18 669 462 109 135 Cuts at: 2456 2722 2884 3027 Size: 266 162 143 Fragments arranged by size: 924 669 462 266 162 143 135 120 109 19 18 SfoI GGC'GCC Cuts at: 0 2184 3027 Size: 2184 843 SmaI CCC'GGG Cuts at: 0 2316 3027 Size: 2316 711 SmlI C'TyrA_G Cuts at: 0 297 937 2784 2864 3027 Size: 297 640 1847 80 163 Fragments arranged by size: 1847 640 297 163 80 SspI AAT'ATT Cuts at: 0 523 3027 Size: 523 2504 StuI AGG'CCT Cuts at: 0 2829 2870 3027 Size: 2829 41 157 Fragments arranged by size: 2829 157 41 StyI C'CwwG_G Cuts at: 0 1433 2106 2274 2699 3027 Size: 1433 673 168 425 328 Fragments arranged by size: 1433 673 425 328 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2481 2541 2722 3027 Size: 2481 60 181 305 Fragments arranged by size: 2481 305 181 60 TatI w'GTAC_w Cuts at: 0 662 677 3027 Size: 662 15 2350 Fragments arranged by size: 2350 662 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 364 396 1024 1056 1218 1250 3027 Size: 364 32 628 32 162 32 1777 Fragments arranged by size: 1777 628 364 162 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2076 2535 2814 3027 Size: 2076 459 279 213 Fragments arranged by size: 2076 459 279 213 XmaI C'CCGG_G Cuts at: 0 2314 3027 Size: 2314 713 ZraI GAC'GTC Cuts at: 0 1556 1719 2199 3027 Size: 1556 163 480 828 Fragments arranged by size: 1556 828 480 163 Enzymes that do cut: AatII AccI AclI AcuI AflII AflIII AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI PstI PvuII SacII SanDI SapI SbfI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AfeI AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI BmtI BsiEI BsiWI BspHI BspMI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PspXI PvuI RsrII SacI SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmnI