Restriction map for
pFN21AE4244
(Linear) (Six-base) MAPSORT of: KIEE4244.seq Check: 1757 from: 1 to: 2727
KIEE4244.orf
With 182 enzymes: SgfI *
July 3, 2012 18:51 ..
AccI GT'mk_AC
Cuts at: 0 639 2727
Size: 639 2088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 155 932 2432 2727
Size: 50 105 777 1500 295
Fragments arranged by size:
1500 777 295 105 50
AfeI AGC'GCT
Cuts at: 0 1189 2727
Size: 1189 1538
AleI CACnn'nnGTG
Cuts at: 0 1139 1151 2727
Size: 1139 12 1576
Fragments arranged by size:
1576 1139 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2204 2727
Size: 2204 523
ApaLI G'TGCA_C
Cuts at: 0 299 2727
Size: 299 2428
ApoI r'AATT_y
Cuts at: 0 213 292 421 439 487 628 849 1246
Size: 213 79 129 18 48 141 221 397
Cuts at: 1246 1351 1593 2024 2096 2727
Size: 105 242 431 72 631
Fragments arranged by size:
631 431 397 242 221 213 141 129
105 79 72 48 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 609 1309 1375 1428 2727
Size: 129 480 700 66 53 1299
Fragments arranged by size:
1299 700 480 129 66 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 551 1732 1766 2727
Size: 517 34 1181 34 961
Fragments arranged by size:
1181 961 517 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2301 2727
Size: 2301 426
BclI T'GATC_A
Cuts at: 0 1845 2727
Size: 1845 882
BfrBI ATG'CAT
Cuts at: 0 2055 2727
Size: 2055 672
Bme1580I G_kGCm'C
Cuts at: 0 303 778 1273 2727
Size: 303 475 495 1454
Fragments arranged by size:
1454 495 475 303
BmrI ACTGGGnnnn_n'
Cuts at: 0 2608 2680 2727
Size: 2608 72 47
Fragments arranged by size:
2608 72 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 751 1031 2727
Size: 155 596 280 1696
Fragments arranged by size:
1696 596 280 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1442 2727
Size: 52 1390 1285
Fragments arranged by size:
1390 1285 52
BsaAI yAC'GTr
Cuts at: 0 390 2727
Size: 390 2337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 922 952 1252 1282 1958 1988 2571 2601
Size: 922 30 300 30 676 30 583 30
Cuts at: 2601 2727
Size: 126
Fragments arranged by size:
922 676 583 300 126 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 943 946 2579 2727
Size: 24 919 3 1633 148
Fragments arranged by size:
1633 919 148 24 3
BsiHKAI G_wGCw'C
Cuts at: 0 303 1265 2727
Size: 303 962 1462
Fragments arranged by size:
1462 962 303
BsmI GAATG_Cn'
Cuts at: 0 2067 2727
Size: 2067 660
Bsp1286I G_dGCh'C
Cuts at: 0 303 778 1265 1273 2727
Size: 303 475 487 8 1454
Fragments arranged by size:
1454 487 475 303 8
BspHI T'CATG_A
Cuts at: 0 1032 1791 1842 2238 2505 2727
Size: 1032 759 51 396 267 222
Fragments arranged by size:
1032 759 396 267 222 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2604 2727
Size: 2604 123
BsrGI T'GTAC_A
Cuts at: 0 449 2727
Size: 449 2278
BssSI C'ACGA_G
Cuts at: 0 2546 2727
Size: 2546 181
BstBI TT'CG_AA
Cuts at: 0 2061 2727
Size: 2061 666
BstYI r'GATC_y
Cuts at: 0 38 2637 2727
Size: 38 2599 90
Fragments arranged by size:
2599 90 38
Bsu36I CC'TnA_GG
Cuts at: 0 1004 2727
Size: 1004 1723
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 815 1829 2727
Size: 815 1014 898
Fragments arranged by size:
1014 898 815
BtsI GCAGTG_nn'
Cuts at: 0 89 515 1090 2727
Size: 89 426 575 1637
Fragments arranged by size:
1637 575 426 89
DraI TTT'AAA
Cuts at: 0 230 1500 2031 2727
Size: 230 1270 531 696
Fragments arranged by size:
1270 696 531 230
EarI CTCTTCn'nnn_
Cuts at: 0 1 146 2727
Size: 1 145 2581
Fragments arranged by size:
2581 145 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 155 751 932 1031 2432 2727
Size: 50 105 596 181 99 1401 295
Fragments arranged by size:
1401 596 295 181 105 99 50
EcoO109I rG'GnC_Cy
Cuts at: 0 692 888 1546 2727
Size: 692 196 658 1181
Fragments arranged by size:
1181 692 658 196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1697 2727
Size: 1697 1030
HaeII r_GCGC'y
Cuts at: 0 1191 2727
Size: 1191 1536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 342 374 922 934 954 966
Size: 93 32 217 32 548 12 20 12
Cuts at: 966 1317 1349 2296 2328 2500 2532 2727
Size: 351 32 947 32 172 32 195
Fragments arranged by size:
947 548 351 217 195 172 93 32
32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 1043 2534 2727
Size: 1043 1491 193
Fragments arranged by size:
1491 1043 193
HindIII A'AGCT_T
Cuts at: 0 184 2727
Size: 184 2543
HpaI GTT'AAC
Cuts at: 0 1043 2727
Size: 1043 1684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 2357 2727
Size: 1366 991 370
Fragments arranged by size:
1366 991 370
MslI CAynn'nnrTG
Cuts at: 0 986 1139 1151 1235 1793 1877 1895 2006
Size: 986 153 12 84 558 84 18 111
Cuts at: 2006 2072 2088 2156 2186 2237 2727
Size: 66 16 68 30 51 490
Fragments arranged by size:
986 558 490 153 111 84 84 68
66 51 30 18 16 12
MspA1I CmG'CkG
Cuts at: 0 2554 2727
Size: 2554 173
NdeI CA'TA_TG
Cuts at: 0 1442 2727
Size: 1442 1285
NsiI A_TGCA'T
Cuts at: 0 2057 2727
Size: 2057 670
NspI r_CATG'y
Cuts at: 0 1321 2727
Size: 1321 1406
PpuMI rG'GwC_Cy
Cuts at: 0 692 888 1546 2727
Size: 692 196 658 1181
Fragments arranged by size:
1181 692 658 196
PsiI TTA'TAA
Cuts at: 0 653 2727
Size: 653 2074
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 2420 2452 2727
Size: 1013 32 1375 32 275
Fragments arranged by size:
1375 1013 275 32 32
PstI C_TGCA'G
Cuts at: 0 120 2727
Size: 120 2607
SapI GCTCTTCn'nnn_
Cuts at: 0 146 2727
Size: 146 2581
ScaI AGT'ACT
Cuts at: 0 949 2271 2308 2727
Size: 949 1322 37 419
Fragments arranged by size:
1322 949 419 37
SexAI A'CCwGG_T
Cuts at: 0 2141 2727
Size: 2141 586
SfcI C'TryA_G
Cuts at: 0 116 633 1019 2727
Size: 116 517 386 1708
Fragments arranged by size:
1708 517 386 116
SmlI C'TyrA_G
Cuts at: 0 31 1457 2727
Size: 31 1426 1270
Fragments arranged by size:
1426 1270 31
SspI AAT'ATT
Cuts at: 0 226 1079 2703 2727
Size: 226 853 1624 24
Fragments arranged by size:
1624 853 226 24
StyI C'CwwG_G
Cuts at: 0 1962 2727
Size: 1962 765
SwaI ATTT'AAAT
Cuts at: 0 230 2727
Size: 230 2497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1127 2627 2727
Size: 1127 1500 100
Fragments arranged by size:
1500 1127 100
TatI w'GTAC_w
Cuts at: 0 449 947 1615 1785 2269 2306 2727
Size: 449 498 668 170 484 37 421
Fragments arranged by size:
668 498 484 449 421 170 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1252 1284 1958 1990 2727
Size: 1252 32 674 32 737
Fragments arranged by size:
1252 737 674 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 140 879 2067 2727
Size: 140 739 1188 660
Fragments arranged by size:
1188 739 660 140
Enzymes that do cut:
AccI AcuI AfeI AleI AlfI ApaLI ApoI BbsI
BcgI BciVI BclI BfrBI Bme1580I BmrI BpmI BpuEI
BsaAI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrGI BssSI BstBI BstYI Bsu36I BtgZI BtsI DraI
EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII
HpaI MmeI MslI MspA1I NdeI NsiI NspI PpuMI
PsiI PsrI PstI SapI ScaI SexAI SfcI SmlI
SspI StyI SwaI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BstAPI BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI Tth111I
XbaI XcmI XhoI XmaI ZraI