Restriction map for
pFN21AE4242
(Linear) (Six-base) MAPSORT of: KIEE4242.seq Check: 884 from: 1 to: 2712
KIEE4242.orf
With 182 enzymes: SgfI *
March 22, 2012 17:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1169 2083 2593 2712
Size: 1124 45 914 510 119
Fragments arranged by size:
1124 914 510 119 45
AhdI GACnn_n'nnGTC
Cuts at: 0 1614 2712
Size: 1614 1098
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1196 1228 2644 2676 2712
Size: 1196 32 1416 32 36
Fragments arranged by size:
1416 1196 36 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1752 2712
Size: 1752 960
ApaI G_GGCC'C
Cuts at: 0 1924 2712
Size: 1924 788
ApoI r'AATT_y
Cuts at: 0 133 566 1089 1641 2712
Size: 133 433 523 552 1071
Fragments arranged by size:
1071 552 523 433 133
AseI AT'TA_AT
Cuts at: 0 1658 2712
Size: 1658 1054
AvrII C'CTAG_G
Cuts at: 0 469 2712
Size: 469 2243
BamHI G'GATC_C
Cuts at: 0 1309 2712
Size: 1309 1403
BanII G_rGCy'C
Cuts at: 0 1924 2712
Size: 1924 788
BbsI GAAGACnn'nnnn_
Cuts at: 0 1601 2712
Size: 1601 1111
BciVI GTATCCnnnnn_n'
Cuts at: 0 1436 2712
Size: 1436 1276
BfrBI ATG'CAT
Cuts at: 0 2599 2712
Size: 2599 113
BglII A'GATC_T
Cuts at: 0 841 2712
Size: 841 1871
BlpI GC'TnA_GC
Cuts at: 0 1624 2098 2712
Size: 1624 474 614
Fragments arranged by size:
1624 614 474
Bme1580I G_kGCm'C
Cuts at: 0 1184 1924 2485 2712
Size: 1184 740 561 227
Fragments arranged by size:
1184 740 561 227
BmgBI CAC'GTC
Cuts at: 0 1367 2712
Size: 1367 1345
BmrI ACTGGGnnnn_n'
Cuts at: 0 1271 2712
Size: 1271 1441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1660 1684 2712
Size: 553 1107 24 1028
Fragments arranged by size:
1107 1028 553 24
BsaAI yAC'GTr
Cuts at: 0 589 1167 1743 2712
Size: 589 578 576 969
Fragments arranged by size:
969 589 578 576
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1931 1961 2712
Size: 1931 30 751
Fragments arranged by size:
1931 751 30
BseYI C'CCAG_C
Cuts at: 0 1220 1305 1923 2712
Size: 1220 85 618 789
Fragments arranged by size:
1220 789 618 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 2049 2712
Size: 1765 284 663
Fragments arranged by size:
1765 663 284
BsmI GAATG_Cn'
Cuts at: 0 2047 2601 2712
Size: 2047 554 111
Fragments arranged by size:
2047 554 111
Bsp1286I G_dGCh'C
Cuts at: 0 1184 1924 2485 2712
Size: 1184 740 561 227
Fragments arranged by size:
1184 740 561 227
BsrDI GCAATG_nn'
Cuts at: 0 434 2379 2531 2712
Size: 434 1945 152 181
Fragments arranged by size:
1945 434 181 152
BstBI TT'CG_AA
Cuts at: 0 2682 2712
Size: 2682 30
BstYI r'GATC_y
Cuts at: 0 841 1309 2712
Size: 841 468 1403
Fragments arranged by size:
1403 841 468
Bsu36I CC'TnA_GG
Cuts at: 0 556 2712
Size: 556 2156
BtgI C'CryG_G
Cuts at: 0 1493 2199 2712
Size: 1493 706 513
Fragments arranged by size:
1493 706 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1049 2712
Size: 1049 1663
BtsI GCAGTG_nn'
Cuts at: 0 2213 2712
Size: 2213 499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1248 1283 2202 2237 2712
Size: 1248 35 919 35 475
Fragments arranged by size:
1248 919 475 35 35
DraI TTT'AAA
Cuts at: 0 1640 2712
Size: 1640 1072
DraIII CAC_nnn'GTG
Cuts at: 0 1935 2712
Size: 1935 777
EaeI y'GGCC_r
Cuts at: 0 215 859 1142 2712
Size: 215 644 283 1570
Fragments arranged by size:
1570 644 283 215
EarI CTCTTCn'nnn_
Cuts at: 0 1338 1444 2059 2455 2712
Size: 1338 106 615 396 257
Fragments arranged by size:
1338 615 396 257 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1124 1169 1660 1684 2083 2593 2712
Size: 553 571 45 491 24 399 510 119
Fragments arranged by size:
571 553 510 491 399 119 45 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1326 1920 2712
Size: 1326 594 792
Fragments arranged by size:
1326 792 594
EcoRI G'AATT_C
Cuts at: 0 133 2712
Size: 133 2579
EcoRV GAT'ATC
Cuts at: 0 2347 2712
Size: 2347 365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2164 2423 2712
Size: 2164 259 289
Fragments arranged by size:
2164 289 259
FspI TGC'GCA
Cuts at: 0 1532 2712
Size: 1532 1180
HaeII r_GCGC'y
Cuts at: 0 429 2712
Size: 429 2283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 826 858 1600 1632 1854 1886 1931 1963
Size: 826 32 742 32 222 32 45 32
Cuts at: 1963 2275 2307 2712
Size: 312 32 405
Fragments arranged by size:
826 742 405 312 222 45 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2196 2712
Size: 2196 516
HindIII A'AGCT_T
Cuts at: 0 713 2712
Size: 713 1999
MfeI C'AATT_G
Cuts at: 0 2219 2712
Size: 2219 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 2712
Size: 421 2291
MscI TGG'CCA
Cuts at: 0 217 1144 2712
Size: 217 927 1568
Fragments arranged by size:
1568 927 217
MslI CAynn'nnrTG
Cuts at: 0 830 1498 2275 2384 2429 2712
Size: 830 668 777 109 45 283
Fragments arranged by size:
830 777 668 283 109 45
MspA1I CmG'CkG
Cuts at: 0 301 314 1224 1422 2712
Size: 301 13 910 198 1290
Fragments arranged by size:
1290 910 301 198 13
NcoI C'CATG_G
Cuts at: 0 1493 2712
Size: 1493 1219
NdeI CA'TA_TG
Cuts at: 0 849 2712
Size: 849 1863
NsiI A_TGCA'T
Cuts at: 0 2601 2712
Size: 2601 111
NspI r_CATG'y
Cuts at: 0 2194 2712
Size: 2194 518
PfoI T'CCnGG_A
Cuts at: 0 883 2712
Size: 883 1829
PmlI CAC'GTG
Cuts at: 0 1167 2712
Size: 1167 1545
PsiI TTA'TAA
Cuts at: 0 91 2712
Size: 91 2621
PspOMI G'GGCC_C
Cuts at: 0 1920 2712
Size: 1920 792
PstI C_TGCA'G
Cuts at: 0 1448 1913 2187 2712
Size: 1448 465 274 525
Fragments arranged by size:
1448 525 465 274
PvuII CAG'CTG
Cuts at: 0 314 1224 1422 2712
Size: 314 910 198 1290
Fragments arranged by size:
1290 910 314 198
SapI GCTCTTCn'nnn_
Cuts at: 0 1444 2712
Size: 1444 1268
ScaI AGT'ACT
Cuts at: 0 2678 2712
Size: 2678 34
SfcI C'TryA_G
Cuts at: 0 233 1360 1444 1858 1909 2183 2639 2712
Size: 233 1127 84 414 51 274 456 73
Fragments arranged by size:
1127 456 414 274 233 84 73 51
SnaBI TAC'GTA
Cuts at: 0 589 2712
Size: 589 2123
SspI AAT'ATT
Cuts at: 0 771 1028 2571 2712
Size: 771 257 1543 141
Fragments arranged by size:
1543 771 257 141
StuI AGG'CCT
Cuts at: 0 1101 2712
Size: 1101 1611
StyI C'CwwG_G
Cuts at: 0 469 619 962 1065 1185 1493 2712
Size: 469 150 343 103 120 308 1219
Fragments arranged by size:
1219 469 343 308 150 120 103
SwaI ATTT'AAAT
Cuts at: 0 1640 2712
Size: 1640 1072
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 2712
Size: 612 2100
TatI w'GTAC_w
Cuts at: 0 2676 2712
Size: 2676 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 2712
Size: 1124 32 1556
Fragments arranged by size:
1556 1124 32
Tth111I GACn'n_nGTC
Cuts at: 0 1368 2712
Size: 1368 1344
XbaI T'CTAG_A
Cuts at: 0 1887 2712
Size: 1887 825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2624 2712
Size: 2624 88
XmnI GAAnn'nnTTC
Cuts at: 0 783 971 2570 2712
Size: 783 188 1599 142
Fragments arranged by size:
1599 783 188 142
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApaI ApoI AseI AvrII
BamHI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BpmI BsaAI BsaXI BseYI BsgI BsmI
Bsp1286I BsrDI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PfoI PmlI PsiI PspOMI PstI PvuII SapI ScaI
SfcI SnaBI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaLI AscI AsiSI AvaI BaeI
BanI BbeI BbvCI BcgI BclI BglI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I ClaI
DrdI EagI EciI EcoICRI EcoNI FseI FspAI HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI XhoI XmaI ZraI