Restriction map for
pFN21AE4240
(Linear) (Six-base) MAPSORT of: KIEE4240.seq Check: 9488 from: 1 to: 2625
KIEE4240.orf
With 182 enzymes: SgfI *
March 22, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 717 1729 1825 2625
Size: 717 1012 96 800
Fragments arranged by size:
1012 800 717 96
AatII G_ACGT'C
Cuts at: 0 1151 2625
Size: 1151 1474
AccI GT'mk_AC
Cuts at: 0 1175 2039 2625
Size: 1175 864 586
Fragments arranged by size:
1175 864 586
Acc65I G'GTAC_C
Cuts at: 0 1844 2625
Size: 1844 781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 1869 2625
Size: 498 1371 756
Fragments arranged by size:
1371 756 498
AfeI AGC'GCT
Cuts at: 0 1798 2625
Size: 1798 827
AflIII A'CryG_T
Cuts at: 0 1967 2198 2267 2625
Size: 1967 231 69 358
Fragments arranged by size:
1967 358 231 69
AhdI GACnn_n'nnGTC
Cuts at: 0 312 2625
Size: 312 2313
AleI CACnn'nnGTG
Cuts at: 0 751 2625
Size: 751 1874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1981 2013 2625
Size: 1981 32 612
Fragments arranged by size:
1981 612 32
AlwNI CAG_nnn'CTG
Cuts at: 0 95 208 324 639 675 696 885 1304
Size: 95 113 116 315 36 21 189 419
Cuts at: 1304 2625
Size: 1321
Fragments arranged by size:
1321 419 315 189 116 113 95 36
21
ApaI G_GGCC'C
Cuts at: 0 584 779 851 2413 2504 2625
Size: 584 195 72 1562 91 121
Fragments arranged by size:
1562 584 195 121 91 72
AvaI C'yCGr_G
Cuts at: 0 108 924 1279 1390 2062 2625
Size: 108 816 355 111 672 563
Fragments arranged by size:
816 672 563 355 111 108
BanI G'GyrC_C
Cuts at: 0 8 174 829 929 1844 2625
Size: 8 166 655 100 915 781
Fragments arranged by size:
915 781 655 166 100 8
BanII G_rGCy'C
Cuts at: 0 584 779 851 1124 1186 1352 1556 2413
Size: 584 195 72 273 62 166 204 857
Cuts at: 2413 2504 2534 2625
Size: 91 30 91
Fragments arranged by size:
857 584 273 204 195 166 91 91
72 62 30
BbvCI CC'TCA_GC
Cuts at: 0 139 620 689 1417 1478 1748 2625
Size: 139 481 69 728 61 270 877
Fragments arranged by size:
877 728 481 270 139 69 61
BclI T'GATC_A
Cuts at: 0 1625 2625
Size: 1625 1000
BglI GCCn_nnn'nGGC
Cuts at: 0 183 1103 2625
Size: 183 920 1522
Fragments arranged by size:
1522 920 183
BglII A'GATC_T
Cuts at: 0 1382 2192 2625
Size: 1382 810 433
Fragments arranged by size:
1382 810 433
BlpI GC'TnA_GC
Cuts at: 0 1550 2625
Size: 1550 1075
Bme1580I G_kGCm'C
Cuts at: 0 179 584 779 834 851 932 2413 2504
Size: 179 405 195 55 17 81 1481 91
Cuts at: 2504 2625
Size: 121
Fragments arranged by size:
1481 405 195 179 121 91 81 55
17
BmrI ACTGGGnnnn_n'
Cuts at: 0 818 2625
Size: 818 1807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 411 2625
Size: 411 2214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1174 1186 1243 1983 2227 2498 2625
Size: 1138 36 12 57 740 244 271 127
Fragments arranged by size:
1138 740 271 244 127 57 36 12
Bpu10I CC'TnA_GC
Cuts at: 0 139 620 689 1417 1478 1748 2625
Size: 139 481 69 728 61 270 877
Fragments arranged by size:
877 728 481 270 139 69 61
BsaAI yAC'GTr
Cuts at: 0 576 2097 2199 2270 2625
Size: 576 1521 102 71 355
Fragments arranged by size:
1521 576 355 102 71
BsaHI Gr'CG_yC
Cuts at: 0 352 1148 1711 2237 2625
Size: 352 796 563 526 388
Fragments arranged by size:
796 563 526 388 352
BsaWI w'CCGG_w
Cuts at: 0 338 2625
Size: 338 2287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 270 300 1168 1198 1474 1504 1981 2011
Size: 270 30 868 30 276 30 477 30
Cuts at: 2011 2471 2501 2625
Size: 460 30 124
Fragments arranged by size:
868 477 460 276 270 124 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1423 1495 2545 2625
Size: 1423 72 1050 80
Fragments arranged by size:
1423 1050 80 72
BseYI C'CCAG_C
Cuts at: 0 44 500 584 803 1588 1793 2508 2625
Size: 44 456 84 219 785 205 715 117
Fragments arranged by size:
785 715 456 219 205 117 84 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 1362 1919 2625
Size: 201 1161 557 706
Fragments arranged by size:
1161 706 557 201
BsiEI CG_ry'CG
Cuts at: 0 1107 1726 2625
Size: 1107 619 899
Fragments arranged by size:
1107 899 619
BsiHKAI G_wGCw'C
Cuts at: 0 1124 1352 1556 2625
Size: 1124 228 204 1069
Fragments arranged by size:
1124 1069 228 204
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1455 2001 2625
Size: 1455 546 624
Fragments arranged by size:
1455 624 546
Bsp1286I G_dGCh'C
Cuts at: 0 179 584 779 834 851 932 1124 1186
Size: 179 405 195 55 17 81 192 62
Cuts at: 1186 1352 1556 2413 2504 2534 2625
Size: 166 204 857 91 30 91
Fragments arranged by size:
857 405 204 195 192 179 166 91
91 81 62 55 30 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 717 1145 1604 1729 1825 2625
Size: 717 428 459 125 96 800
Fragments arranged by size:
800 717 459 428 125 96
BsrFI r'CCGG_y
Cuts at: 0 115 223 2233 2625
Size: 115 108 2010 392
Fragments arranged by size:
2010 392 115 108
BssSI C'ACGA_G
Cuts at: 0 250 2625
Size: 250 2375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 885 1265 2625
Size: 885 380 1360
Fragments arranged by size:
1360 885 380
BstBI TT'CG_AA
Cuts at: 0 23 2625
Size: 23 2602
BstEII G'GTnAC_C
Cuts at: 0 273 441 552 2625
Size: 273 168 111 2073
Fragments arranged by size:
2073 273 168 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 753 900 2625
Size: 252 501 147 1725
Fragments arranged by size:
1725 501 252 147
BstYI r'GATC_y
Cuts at: 0 1382 1412 2192 2625
Size: 1382 30 780 433
Fragments arranged by size:
1382 780 433 30
BtgI C'CryG_G
Cuts at: 0 170 436 446 870 1322 1562 2625
Size: 170 266 10 424 452 240 1063
Fragments arranged by size:
1063 452 424 266 240 170 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1425 2625
Size: 1425 1200
BtsI GCAGTG_nn'
Cuts at: 0 105 562 709 1216 1345 2448 2499 2625
Size: 105 457 147 507 129 1103 51 126
Fragments arranged by size:
1103 507 457 147 129 126 105 51
DraIII CAC_nnn'GTG
Cuts at: 0 2180 2625
Size: 2180 445
EaeI y'GGCC_r
Cuts at: 0 254 452 698 762 1095 1104 1202 1229
Size: 254 198 246 64 333 9 98 27
Cuts at: 1229 1295 1319 1325 1361 1397 1559 1723 1808
Size: 66 24 6 36 36 162 164 85
Cuts at: 1808 2625
Size: 817
Fragments arranged by size:
817 333 254 246 198 164 162 98
85 66 64 36 36 27 24 9
6
EagI C'GGCC_G
Cuts at: 0 1104 1723 2625
Size: 1104 619 902
Fragments arranged by size:
1104 902 619
EarI CTCTTCn'nnn_
Cuts at: 0 1613 2625
Size: 1613 1012
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 801 1558 2625
Size: 801 757 1067
Fragments arranged by size:
1067 801 757
EcoICRI GAG'CTC
Cuts at: 0 1122 1350 1554 2625
Size: 1122 228 204 1071
Fragments arranged by size:
1122 1071 228 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 1138 1174 1186 1243 1869 1983 2227
Size: 498 640 36 12 57 626 114 244
Cuts at: 2227 2498 2625
Size: 271 127
Fragments arranged by size:
640 626 498 271 244 127 114 57
36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2390 2625
Size: 2390 235
EcoO109I rG'GnC_Cy
Cuts at: 0 537 580 581 672 742 775 776 848
Size: 537 43 1 91 70 33 1 72
Cuts at: 848 1065 1521 1650 2500 2625
Size: 217 456 129 850 125
Fragments arranged by size:
850 537 456 217 129 125 91 72
70 43 33 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1452 2625
Size: 1452 1173
HaeII r_GCGC'y
Cuts at: 0 1800 2625
Size: 1800 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 405 437 2471 2503 2625
Size: 298 32 75 32 2034 32 122
Fragments arranged by size:
2034 298 122 75 32 32 32
HincII GTy'rAC
Cuts at: 0 69 657 1611 2625
Size: 69 588 954 1014
Fragments arranged by size:
1014 954 588 69
KpnI G_GTAC'C
Cuts at: 0 1848 2625
Size: 1848 777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 2625
Size: 1478 1147
MscI TGG'CCA
Cuts at: 0 256 1321 1363 2625
Size: 256 1065 42 1262
Fragments arranged by size:
1262 1065 256 42
MslI CAynn'nnrTG
Cuts at: 0 488 550 751 898 1031 2625
Size: 488 62 201 147 133 1594
Fragments arranged by size:
1594 488 201 147 133 62
MspA1I CmG'CkG
Cuts at: 0 165 594 751 1094 1290 2018 2317 2625
Size: 165 429 157 343 196 728 299 308
Fragments arranged by size:
728 429 343 308 299 196 165 157
NaeI GCC'GGC
Cuts at: 0 2235 2625
Size: 2235 390
NcoI C'CATG_G
Cuts at: 0 436 1322 2625
Size: 436 886 1303
Fragments arranged by size:
1303 886 436
NdeI CA'TA_TG
Cuts at: 0 1679 2625
Size: 1679 946
NgoMIV G'CCGG_C
Cuts at: 0 2233 2625
Size: 2233 392
NspI r_CATG'y
Cuts at: 0 663 1971 2568 2625
Size: 663 1308 597 57
Fragments arranged by size:
1308 663 597 57
PasI CC'CwG_GG
Cuts at: 0 318 374 2625
Size: 318 56 2251
Fragments arranged by size:
2251 318 56
PciI A'CATG_T
Cuts at: 0 1967 2625
Size: 1967 658
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 374 2126 2270 2546 2625
Size: 324 50 1752 144 276 79
Fragments arranged by size:
1752 324 276 144 79 50
PfoI T'CCnGG_A
Cuts at: 0 1151 2625
Size: 1151 1474
PmlI CAC'GTG
Cuts at: 0 576 2097 2270 2625
Size: 576 1521 173 355
Fragments arranged by size:
1521 576 355 173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1981 2013 2625
Size: 1981 32 612
Fragments arranged by size:
1981 612 32
PpuMI rG'GwC_Cy
Cuts at: 0 742 1065 1521 1650 2625
Size: 742 323 456 129 975
Fragments arranged by size:
975 742 456 323 129
PspOMI G'GGCC_C
Cuts at: 0 580 775 847 2409 2500 2625
Size: 580 195 72 1562 91 125
Fragments arranged by size:
1562 580 195 125 91 72
PstI C_TGCA'G
Cuts at: 0 100 881 1051 1142 1382 1740 2172 2443
Size: 100 781 170 91 240 358 432 271
Cuts at: 2443 2625
Size: 182
Fragments arranged by size:
781 432 358 271 240 182 170 100
91
PvuII CAG'CTG
Cuts at: 0 1290 2625
Size: 1290 1335
SacI G_AGCT'C
Cuts at: 0 1124 1352 1556 2625
Size: 1124 228 204 1069
Fragments arranged by size:
1124 1069 228 204
SapI GCTCTTCn'nnn_
Cuts at: 0 1613 2625
Size: 1613 1012
SbfI CC_TGCA'GG
Cuts at: 0 1142 2625
Size: 1142 1483
SexAI A'CCwGG_T
Cuts at: 0 1652 2625
Size: 1652 973
SfcI C'TryA_G
Cuts at: 0 96 200 877 1047 1138 1378 1736 2168
Size: 96 104 677 170 91 240 358 432
Cuts at: 2168 2439 2625
Size: 271 186
Fragments arranged by size:
677 432 358 271 240 186 170 104
96 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1103 2625
Size: 1103 1522
SmaI CCC'GGG
Cuts at: 0 926 2625
Size: 926 1699
SmlI C'TyrA_G
Cuts at: 0 1279 2625
Size: 1279 1346
StuI AGG'CCT
Cuts at: 0 1369 2524 2625
Size: 1369 1155 101
Fragments arranged by size:
1369 1155 101
StyI C'CwwG_G
Cuts at: 0 281 436 1322 1364 1427 1472 1850 2150
Size: 281 155 886 42 63 45 378 300
Cuts at: 2150 2625
Size: 475
Fragments arranged by size:
886 475 378 300 281 155 63 45
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 429 2625
Size: 429 2196
TatI w'GTAC_w
Cuts at: 0 2157 2625
Size: 2157 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 2060 2092 2625
Size: 270 32 1758 32 533
Fragments arranged by size:
1758 533 270 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 1305 2625
Size: 1218 87 1320
Fragments arranged by size:
1320 1218 87
XhoI C'TCGA_G
Cuts at: 0 1279 2625
Size: 1279 1346
XmaI C'CCGG_G
Cuts at: 0 924 2625
Size: 924 1701
ZraI GAC'GTC
Cuts at: 0 1149 2625
Size: 1149 1476
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AloI AlwNI ApaI AvaI BanI BanII BbvCI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SapI SbfI SexAI SfcI SfiI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AlfI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BfrBI BmgBI BmtI BpuEI BsaI BsaBI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NarI NheI
NotI NruI NsiI PacI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmnI