(Linear) (Six-base) MAPSORT of: KIEE4230.seq Check: 1546 from: 1 to: 2424 KIEE4230.orf With 182 enzymes: SgfI * July 3, 2012 18:50 .. AccI GT'mk_AC Cuts at: 0 1460 2424 Size: 1460 964 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 18 598 893 911 1328 1466 2424 Size: 18 580 295 18 417 138 958 Fragments arranged by size: 958 580 417 295 138 18 18 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 521 2297 2424 Size: 521 1776 127 Fragments arranged by size: 1776 521 127 AlwNI CAG_nnn'CTG Cuts at: 0 51 1864 1909 2424 Size: 51 1813 45 515 Fragments arranged by size: 1813 515 51 45 ApaLI G'TGCA_C Cuts at: 0 1604 2424 Size: 1604 820 ApoI r'AATT_y Cuts at: 0 990 1344 1398 1612 2232 2370 2424 Size: 990 354 54 214 620 138 54 Fragments arranged by size: 990 620 354 214 138 54 54 AvaI C'yCGr_G Cuts at: 0 462 666 753 1129 2424 Size: 462 204 87 376 1295 Fragments arranged by size: 1295 462 376 204 87 BanI G'GyrC_C Cuts at: 0 513 1294 2116 2424 Size: 513 781 822 308 Fragments arranged by size: 822 781 513 308 BanII G_rGCy'C Cuts at: 0 1046 1198 1226 1668 2424 Size: 1046 152 28 442 756 Fragments arranged by size: 1046 756 442 152 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 827 2131 2424 Size: 827 1304 293 Fragments arranged by size: 1304 827 293 BciVI GTATCCnnnnn_n' Cuts at: 0 1306 2424 Size: 1306 1118 BfrBI ATG'CAT Cuts at: 0 1431 2424 Size: 1431 993 BglII A'GATC_T Cuts at: 0 1680 2109 2424 Size: 1680 429 315 Fragments arranged by size: 1680 429 315 Bme1580I G_kGCm'C Cuts at: 0 1608 2424 Size: 1608 816 BmrI ACTGGGnnnn_n' Cuts at: 0 879 1040 1588 2424 Size: 879 161 548 836 Fragments arranged by size: 879 836 548 161 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1527 1968 2424 Size: 1527 441 456 Fragments arranged by size: 1527 456 441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 362 1963 2041 2424 Size: 362 1601 78 383 Fragments arranged by size: 1601 383 362 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 719 1903 2424 Size: 719 1184 521 Fragments arranged by size: 1184 719 521 BsaI GGTCTCn'nnnn_ Cuts at: 0 113 656 1784 2424 Size: 113 543 1128 640 Fragments arranged by size: 1128 640 543 113 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1254 1284 2424 Size: 1254 30 1140 Fragments arranged by size: 1254 1140 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 235 444 1722 1960 1972 1975 2424 Size: 235 209 1278 238 12 3 449 Fragments arranged by size: 1278 449 238 235 209 12 3 BseYI C'CCAG_C Cuts at: 0 364 1105 2424 Size: 364 741 1319 Fragments arranged by size: 1319 741 364 BsiHKAI G_wGCw'C Cuts at: 0 675 1608 2424 Size: 675 933 816 Fragments arranged by size: 933 816 675 BsmI GAATG_Cn' Cuts at: 0 1431 2424 Size: 1431 993 BsmBI CGTCTCn'nnnn_ Cuts at: 0 93 2424 Size: 93 2331 Bsp1286I G_dGCh'C Cuts at: 0 675 1046 1198 1226 1608 1668 2424 Size: 675 371 152 28 382 60 756 Fragments arranged by size: 756 675 382 371 152 60 28 BspHI T'CATG_A Cuts at: 0 2291 2424 Size: 2291 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 182 2424 Size: 182 2242 BsrBI CCG'CTC Cuts at: 0 1095 2424 Size: 1095 1329 BsrDI GCAATG_nn' Cuts at: 0 1077 1082 2424 Size: 1077 5 1342 Fragments arranged by size: 1342 1077 5 BsrFI r'CCGG_y Cuts at: 0 516 2424 Size: 516 1908 BstAPI GCAn_nnn'nTGC Cuts at: 0 2151 2424 Size: 2151 273 BstYI r'GATC_y Cuts at: 0 175 1680 2109 2424 Size: 175 1505 429 315 Fragments arranged by size: 1505 429 315 175 Bsu36I CC'TnA_GG Cuts at: 0 427 974 1658 2424 Size: 427 547 684 766 Fragments arranged by size: 766 684 547 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 53 878 2424 Size: 53 825 1546 Fragments arranged by size: 1546 825 53 BtsI GCAGTG_nn' Cuts at: 0 505 1182 1820 2424 Size: 505 677 638 604 Fragments arranged by size: 677 638 604 505 DraI TTT'AAA Cuts at: 0 1331 2424 Size: 1331 1093 EarI CTCTTCn'nnn_ Cuts at: 0 111 574 849 869 2424 Size: 111 463 275 20 1555 Fragments arranged by size: 1555 463 275 111 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 18 362 598 893 911 1328 1466 1963 Size: 18 344 236 295 18 417 138 497 Cuts at: 1963 2041 2424 Size: 78 383 Fragments arranged by size: 497 417 383 344 295 236 138 78 18 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 706 1945 2424 Size: 706 1239 479 Fragments arranged by size: 1239 706 479 EcoRI G'AATT_C Cuts at: 0 1398 2232 2424 Size: 1398 834 192 Fragments arranged by size: 1398 834 192 EcoRV GAT'ATC Cuts at: 0 260 2424 Size: 260 2164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 425 457 573 605 1132 1164 Size: 289 32 104 32 116 32 527 32 Cuts at: 1164 1201 1233 2314 2346 2424 Size: 37 32 1081 32 78 Fragments arranged by size: 1081 527 289 116 104 78 37 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2160 2424 Size: 2160 264 MfeI C'AATT_G Cuts at: 0 293 2424 Size: 293 2131 MslI CAynn'nnrTG Cuts at: 0 270 1766 2267 2424 Size: 270 1496 501 157 Fragments arranged by size: 1496 501 270 157 NsiI A_TGCA'T Cuts at: 0 1433 2424 Size: 1433 991 NspI r_CATG'y Cuts at: 0 63 1771 2424 Size: 63 1708 653 Fragments arranged by size: 1708 653 63 PasI CC'CwG_GG Cuts at: 0 524 2424 Size: 524 1900 PfoI T'CCnGG_A Cuts at: 0 1940 2018 2424 Size: 1940 78 406 Fragments arranged by size: 1940 406 78 PshAI GACnn'nnGTC Cuts at: 0 1973 2424 Size: 1973 451 PstI C_TGCA'G Cuts at: 0 2051 2424 Size: 2051 373 SexAI A'CCwGG_T Cuts at: 0 2246 2309 2424 Size: 2246 63 115 Fragments arranged by size: 2246 115 63 SfcI C'TryA_G Cuts at: 0 779 1154 2047 2424 Size: 779 375 893 377 Fragments arranged by size: 893 779 377 375 SmaI CCC'GGG Cuts at: 0 1131 2424 Size: 1131 1293 SmlI C'TyrA_G Cuts at: 0 734 1918 2424 Size: 734 1184 506 Fragments arranged by size: 1184 734 506 StuI AGG'CCT Cuts at: 0 557 712 1290 2150 2424 Size: 557 155 578 860 274 Fragments arranged by size: 860 578 557 274 155 StyI C'CwwG_G Cuts at: 0 241 717 2424 Size: 241 476 1707 Fragments arranged by size: 1707 476 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 786 1312 2155 2424 Size: 786 526 843 269 Fragments arranged by size: 843 786 526 269 XmaI C'CCGG_G Cuts at: 0 1129 2424 Size: 1129 1295 XmnI GAAnn'nnTTC Cuts at: 0 31 2424 Size: 31 2393 Enzymes that do cut: AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BciVI BfrBI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstYI Bsu36I BtgZI BtsI DraI EarI Eco57MI EcoNI EcoRI EcoRV Hin4I HincII MfeI MslI NsiI NspI PasI PfoI PshAI PstI SexAI SfcI SmaI SmlI StuI StyI TaqII XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI