Restriction map for
pFN21AE4225
(Linear) (Six-base) MAPSORT of: KIEE4225.seq Check: 9872 from: 1 to: 2382
KIEE4225.orf
With 182 enzymes: SgfI *
July 3, 2012 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 67 927 2382
Size: 67 860 1455
Fragments arranged by size:
1455 860 67
AccI GT'mk_AC
Cuts at: 0 566 2382
Size: 566 1816
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1543 2116 2137 2382
Size: 1543 573 21 245
Fragments arranged by size:
1543 573 245 21
AflIII A'CryG_T
Cuts at: 0 254 2382
Size: 254 2128
AhdI GACnn_n'nnGTC
Cuts at: 0 156 1368 2382
Size: 156 1212 1014
Fragments arranged by size:
1212 1014 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2006 2382
Size: 2006 376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 403 435 481 513 895 927
Size: 181 32 190 32 46 32 382 32
Cuts at: 927 2382
Size: 1455
Fragments arranged by size:
1455 382 190 181 46 32 32 32
32
AlwNI CAG_nnn'CTG
Cuts at: 0 381 1716 1839 2373 2382
Size: 381 1335 123 534 9
Fragments arranged by size:
1335 534 381 123 9
ApaI G_GGCC'C
Cuts at: 0 1431 2125 2382
Size: 1431 694 257
Fragments arranged by size:
1431 694 257
AvaI C'yCGr_G
Cuts at: 0 715 869 2382
Size: 715 154 1513
Fragments arranged by size:
1513 715 154
BamHI G'GATC_C
Cuts at: 0 260 2382
Size: 260 2122
BanII G_rGCy'C
Cuts at: 0 10 22 692 716 722 1431 1595 2075
Size: 10 12 670 24 6 709 164 480
Cuts at: 2075 2125 2382
Size: 50 257
Fragments arranged by size:
709 670 480 257 164 50 24 12
10 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1367 2382
Size: 1367 1015
BbvCI CC'TCA_GC
Cuts at: 0 88 2382
Size: 88 2294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 453 487 2382
Size: 453 34 1895
Fragments arranged by size:
1895 453 34
BglI GCCn_nnn'nGGC
Cuts at: 0 49 1618 2382
Size: 49 1569 764
Fragments arranged by size:
1569 764 49
BlpI GC'TnA_GC
Cuts at: 0 1873 2161 2382
Size: 1873 288 221
Fragments arranged by size:
1873 288 221
Bme1580I G_kGCm'C
Cuts at: 0 1431 2125 2382
Size: 1431 694 257
Fragments arranged by size:
1431 694 257
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 1432 2382
Size: 978 454 950
Fragments arranged by size:
978 950 454
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 29 168 940 2343 2382
Size: 29 139 772 1403 39
Fragments arranged by size:
1403 772 139 39 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 163 265 922 1108 1399 1411 2020
Size: 124 39 102 657 186 291 12 609
Cuts at: 2020 2317 2382
Size: 297 65
Fragments arranged by size:
657 609 297 291 186 124 102 65
39 12
Bpu10I CC'TnA_GC
Cuts at: 0 88 235 2382
Size: 88 147 2147
Fragments arranged by size:
2147 147 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 415 1516 1777 2059 2382
Size: 307 108 1101 261 282 323
Fragments arranged by size:
1101 323 307 282 261 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 1375 2382
Size: 1375 1007
BsaAI yAC'GTr
Cuts at: 0 257 2382
Size: 257 2125
BsaBI GATnn'nnATC
Cuts at: 0 1138 2382
Size: 1138 1244
BsaHI Gr'CG_yC
Cuts at: 0 2339 2382
Size: 2339 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 181 211 241 271 403 433
Size: 31 30 120 30 30 30 132 30
Cuts at: 433 895 925 964 994 1081 1111 2382
Size: 462 30 39 30 87 30 1271
Fragments arranged by size:
1271 462 132 120 87 39 31 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 189 207 216 262 307 607 649
Size: 18 171 18 9 46 45 300 42
Cuts at: 649 652 1141 1243 1531 1936 2104 2224 2382
Size: 3 489 102 288 405 168 120 158
Fragments arranged by size:
489 405 300 288 171 168 158 120
102 46 45 42 18 18 9 3
BseYI C'CCAG_C
Cuts at: 0 700 2382
Size: 700 1682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1296 1829 2382
Size: 405 891 533 553
Fragments arranged by size:
891 553 533 405
BsiEI CG_ry'CG
Cuts at: 0 1289 1632 2305 2382
Size: 1289 343 673 77
Fragments arranged by size:
1289 673 343 77
BsiHKAI G_wGCw'C
Cuts at: 0 692 716 1315 1595 2075 2382
Size: 692 24 599 280 480 307
Fragments arranged by size:
692 599 480 307 280 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1405 1753 2382
Size: 1405 348 629
Fragments arranged by size:
1405 629 348
Bsp1286I G_dGCh'C
Cuts at: 0 10 22 692 716 722 1315 1431 1595
Size: 10 12 670 24 6 593 116 164
Cuts at: 1595 2075 2125 2382
Size: 480 50 257
Fragments arranged by size:
670 593 480 257 164 116 50 24
12 10 6
BspHI T'CATG_A
Cuts at: 0 545 2382
Size: 545 1837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 927 1304 2382
Size: 67 860 377 1078
Fragments arranged by size:
1078 860 377 67
BsrBI CCG'CTC
Cuts at: 0 2301 2382
Size: 2301 81
BsrDI GCAATG_nn'
Cuts at: 0 1988 2382
Size: 1988 394
BsrFI r'CCGG_y
Cuts at: 0 134 677 1163 2304 2382
Size: 134 543 486 1141 78
Fragments arranged by size:
1141 543 486 134 78
BssHII G'CGCG_C
Cuts at: 0 1809 2278 2382
Size: 1809 469 104
Fragments arranged by size:
1809 469 104
BssSI C'ACGA_G
Cuts at: 0 493 2382
Size: 493 1889
BstYI r'GATC_y
Cuts at: 0 260 2003 2382
Size: 260 1743 379
Fragments arranged by size:
1743 379 260
Bsu36I CC'TnA_GG
Cuts at: 0 632 2382
Size: 632 1750
BtgI C'CryG_G
Cuts at: 0 392 1623 1648 2382
Size: 392 1231 25 734
Fragments arranged by size:
1231 734 392 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 456 2039 2288 2382
Size: 456 1583 249 94
Fragments arranged by size:
1583 456 249 94
BtsI GCAGTG_nn'
Cuts at: 0 1279 2382
Size: 1279 1103
DrdI GACnn_nn'nnGTC
Cuts at: 0 403 1036 2382
Size: 403 633 1346
Fragments arranged by size:
1346 633 403
EaeI y'GGCC_r
Cuts at: 0 113 707 1286 1629 1718 1970 2302 2306
Size: 113 594 579 343 89 252 332 4
Cuts at: 2306 2351 2382
Size: 45 31
Fragments arranged by size:
594 579 343 332 252 113 89 45
31 4
EagI C'GGCC_G
Cuts at: 0 1286 1629 2302 2382
Size: 1286 343 673 80
Fragments arranged by size:
1286 673 343 80
EarI CTCTTCn'nnn_
Cuts at: 0 119 541 697 1075 1105 2382
Size: 119 422 156 378 30 1277
Fragments arranged by size:
1277 422 378 156 119 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 201 964 1240 1819 1966 2278 2382
Size: 201 763 276 579 147 312 104
Fragments arranged by size:
763 579 312 276 201 147 104
EcoICRI GAG'CTC
Cuts at: 0 690 714 1593 2073 2382
Size: 690 24 879 480 309
Fragments arranged by size:
879 690 480 309 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 163 265 922 1108 1399 1411 1543
Size: 124 39 102 657 186 291 12 132
Cuts at: 1543 2020 2116 2137 2317 2382
Size: 477 96 21 180 65
Fragments arranged by size:
657 477 291 186 180 132 124 102
96 65 39 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 523 934 1195 1300 1720 2382
Size: 247 276 411 261 105 420 662
Fragments arranged by size:
662 420 411 276 261 247 105
EcoO109I rG'GnC_Cy
Cuts at: 0 42 531 1293 1427 1428 1713 2121 2139
Size: 42 489 762 134 1 285 408 18
Cuts at: 2139 2382
Size: 243
Fragments arranged by size:
762 489 408 285 243 134 42 18
1
FseI GG_CCGG'CC
Cuts at: 0 2308 2382
Size: 2308 74
HaeII r_GCGC'y
Cuts at: 0 392 599 1531 1819 2237 2382
Size: 392 207 932 288 418 145
Fragments arranged by size:
932 418 392 288 207 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 31 48 63 142 174 964 996
Size: 16 15 17 15 79 32 790 32
Cuts at: 996 1354 1386 1680 1712 2173 2205 2382
Size: 358 32 294 32 461 32 177
Fragments arranged by size:
790 461 358 294 177 79 32 32
32 32 32 17 16 15 15
HindIII A'AGCT_T
Cuts at: 0 792 2382
Size: 792 1590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 2183 2382
Size: 254 1929 199
Fragments arranged by size:
1929 254 199
MscI TGG'CCA
Cuts at: 0 115 2382
Size: 115 2267
MslI CAynn'nnrTG
Cuts at: 0 397 625 805 886 2382
Size: 397 228 180 81 1496
Fragments arranged by size:
1496 397 228 180 81
MspA1I CmG'CkG
Cuts at: 0 963 1022 1179 1509 1584 1625 1634 2037
Size: 963 59 157 330 75 41 9 403
Cuts at: 2037 2145 2382
Size: 108 237
Fragments arranged by size:
963 403 330 237 157 108 75 59
41 9
NaeI GCC'GGC
Cuts at: 0 2306 2382
Size: 2306 76
NgoMIV G'CCGG_C
Cuts at: 0 2304 2382
Size: 2304 78
NotI GC'GGCC_GC
Cuts at: 0 1629 2382
Size: 1629 753
NspI r_CATG'y
Cuts at: 0 402 747 2382
Size: 402 345 1635
Fragments arranged by size:
1635 402 345
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 371 1554 1716 2373 2382
Size: 287 84 1183 162 657 9
Fragments arranged by size:
1183 657 287 162 84 9
PfoI T'CCnGG_A
Cuts at: 0 140 2382
Size: 140 2242
PmlI CAC'GTG
Cuts at: 0 257 2382
Size: 257 2125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 403 435 895 927 2382
Size: 181 32 190 32 460 32 1455
Fragments arranged by size:
1455 460 190 181 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 531 1293 2139 2382
Size: 531 762 846 243
Fragments arranged by size:
846 762 531 243
PspOMI G'GGCC_C
Cuts at: 0 1427 2121 2382
Size: 1427 694 261
Fragments arranged by size:
1427 694 261
PstI C_TGCA'G
Cuts at: 0 752 938 1301 1514 1583 1589 1676 1823
Size: 752 186 363 213 69 6 87 147
Cuts at: 1823 1835 1865 2246 2382
Size: 12 30 381 136
Fragments arranged by size:
752 381 363 213 186 147 136 87
69 30 12 6
PvuII CAG'CTG
Cuts at: 0 963 1022 1509 1584 2037 2382
Size: 963 59 487 75 453 345
Fragments arranged by size:
963 487 453 345 75 59
SacI G_AGCT'C
Cuts at: 0 692 716 1595 2075 2382
Size: 692 24 879 480 307
Fragments arranged by size:
879 692 480 307 24
SacII CC_GC'GG
Cuts at: 0 1626 2382
Size: 1626 756
SapI GCTCTTCn'nnn_
Cuts at: 0 119 697 1105 2382
Size: 119 578 408 1277
Fragments arranged by size:
1277 578 408 119
SbfI CC_TGCA'GG
Cuts at: 0 2246 2382
Size: 2246 136
ScaI AGT'ACT
Cuts at: 0 418 988 2382
Size: 418 570 1394
Fragments arranged by size:
1394 570 418
SexAI A'CCwGG_T
Cuts at: 0 1052 2382
Size: 1052 1330
SfcI C'TryA_G
Cuts at: 0 748 934 1297 1327 1510 1579 1585 1672
Size: 748 186 363 30 183 69 6 87
Cuts at: 1672 1819 1831 1861 2242 2361 2382
Size: 147 12 30 381 119 21
Fragments arranged by size:
748 381 363 186 183 147 119 87
69 30 30 21 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 1618 2382
Size: 49 1569 764
Fragments arranged by size:
1569 764 49
SmaI CCC'GGG
Cuts at: 0 871 2382
Size: 871 1511
SmlI C'TyrA_G
Cuts at: 0 322 430 1531 1756 2074 2382
Size: 322 108 1101 225 318 308
Fragments arranged by size:
1101 322 318 308 225 108
StuI AGG'CCT
Cuts at: 0 643 2382
Size: 643 1739
StyI C'CwwG_G
Cuts at: 0 2309 2382
Size: 2309 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1399 2382
Size: 790 609 983
Fragments arranged by size:
983 790 609
TatI w'GTAC_w
Cuts at: 0 416 740 986 1061 2060 2382
Size: 416 324 246 75 999 322
Fragments arranged by size:
999 416 324 322 246 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 769 801 2382
Size: 241 32 496 32 1581
Fragments arranged by size:
1581 496 241 32 32
XmaI C'CCGG_G
Cuts at: 0 869 2382
Size: 869 1513
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI AvaI BamHI BanII BbsI BbvCI BcgI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HindIII MmeI MscI MslI MspA1I
NaeI NgoMIV NotI NspI PflMI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI
ScaI SexAI SfcI SfiI SmaI SmlI StuI StyI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI
BciVI BclI BfrBI BglII BmgBI BmtI BsaWI BsiWI
BsmI BspEI BsrGI BstAPI BstBI BstEII BstXI BstZ17I
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NruI NsiI PacI PasI PciI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI