Restriction map for
pFN21AE4221
(Linear) (Six-base) MAPSORT of: KIEE4221.seq Check: 6241 from: 1 to: 2301
KIEE4221.orf
With 182 enzymes: SgfI *
March 22, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 0 1967 2301
Size: 1967 334
AccI GT'mk_AC
Cuts at: 0 562 2301
Size: 562 1739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 2301
Size: 921 1380
AfeI AGC'GCT
Cuts at: 0 1871 2301
Size: 1871 430
AflIII A'CryG_T
Cuts at: 0 1040 2301
Size: 1040 1261
AhdI GACnn_n'nnGTC
Cuts at: 0 569 1032 2301
Size: 569 463 1269
Fragments arranged by size:
1269 569 463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1087 2301
Size: 1087 1214
AlwNI CAG_nnn'CTG
Cuts at: 0 162 1779 1980 2301
Size: 162 1617 201 321
Fragments arranged by size:
1617 321 201 162
ApaI G_GGCC'C
Cuts at: 0 1899 2266 2301
Size: 1899 367 35
Fragments arranged by size:
1899 367 35
ApaLI G'TGCA_C
Cuts at: 0 136 2172 2301
Size: 136 2036 129
Fragments arranged by size:
2036 136 129
AvaI C'yCGr_G
Cuts at: 0 298 332 610 1162 1420 1700 1858 2301
Size: 298 34 278 552 258 280 158 443
Fragments arranged by size:
552 443 298 280 278 258 158 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1714 1747 2301
Size: 1714 33 554
Fragments arranged by size:
1714 554 33
BamHI G'GATC_C
Cuts at: 0 1544 2093 2301
Size: 1544 549 208
Fragments arranged by size:
1544 549 208
BanI G'GyrC_C
Cuts at: 0 321 514 1611 2301
Size: 321 193 1097 690
Fragments arranged by size:
1097 690 321 193
BanII G_rGCy'C
Cuts at: 0 307 355 1899 2042 2266 2301
Size: 307 48 1544 143 224 35
Fragments arranged by size:
1544 307 224 143 48 35
BbeI G_GCGC'C
Cuts at: 0 1615 2301
Size: 1615 686
BbsI GAAGACnn'nnnn_
Cuts at: 0 1520 2301
Size: 1520 781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 369 403 2046 2080 2301
Size: 369 34 1643 34 221
Fragments arranged by size:
1643 369 221 34 34
BfrBI ATG'CAT
Cuts at: 0 1112 2301
Size: 1112 1189
BglI GCCn_nnn'nGGC
Cuts at: 0 150 335 1894 1945 2301
Size: 150 185 1559 51 356
Fragments arranged by size:
1559 356 185 150 51
Bme1580I G_kGCm'C
Cuts at: 0 140 519 1899 2176 2218 2266 2301
Size: 140 379 1380 277 42 48 35
Fragments arranged by size:
1380 379 277 140 48 42 35
BmgBI CAC'GTC
Cuts at: 0 1041 1340 1473 2301
Size: 1041 299 133 828
Fragments arranged by size:
1041 828 299 133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 244 648 2301
Size: 244 404 1653
Fragments arranged by size:
1653 404 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 2301
Size: 895 1406
Bpu10I CC'TnA_GC
Cuts at: 0 354 2301
Size: 354 1947
BsaI GGTCTCn'nnnn_
Cuts at: 0 1788 2301
Size: 1788 513
BsaAI yAC'GTr
Cuts at: 0 1761 2301
Size: 1761 540
BsaHI Gr'CG_yC
Cuts at: 0 797 1190 1612 1964 2272 2301
Size: 797 393 422 352 308 29
Fragments arranged by size:
797 422 393 352 308 29
BsaWI w'CCGG_w
Cuts at: 0 1478 2048 2301
Size: 1478 570 253
Fragments arranged by size:
1478 570 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 682 712 904 934 1159 1189
Size: 358 30 294 30 192 30 225 30
Cuts at: 1189 1225 1255 2137 2167 2301
Size: 36 30 882 30 134
Fragments arranged by size:
882 358 294 225 192 134 36 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 289 496 604 637 690 1180 1249
Size: 21 268 207 108 33 53 490 69
Cuts at: 1249 1252 1360 1438 1554 2073 2301
Size: 3 108 78 116 519 228
Fragments arranged by size:
519 490 268 228 207 116 108 108
78 69 53 33 21 3
BseYI C'CCAG_C
Cuts at: 0 162 216 518 845 1221 1788 1892 2301
Size: 162 54 302 327 376 567 104 409
Fragments arranged by size:
567 409 376 327 302 162 104 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1047 1471 1987 2092 2301
Size: 936 111 424 516 105 209
Fragments arranged by size:
936 516 424 209 111 105
BsiHKAI G_wGCw'C
Cuts at: 0 140 173 307 355 1430 2042 2176 2301
Size: 140 33 134 48 1075 612 134 125
Fragments arranged by size:
1075 612 140 134 134 125 48 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1147 1197 2301
Size: 1147 50 1104
Fragments arranged by size:
1147 1104 50
Bsp1286I G_dGCh'C
Cuts at: 0 140 173 307 355 519 1430 1899 2042
Size: 140 33 134 48 164 911 469 143
Cuts at: 2042 2176 2218 2266 2301
Size: 134 42 48 35
Fragments arranged by size:
911 469 164 143 140 134 134 48
48 42 35 33
BspEI T'CCGG_A
Cuts at: 0 1478 2048 2301
Size: 1478 570 253
Fragments arranged by size:
1478 570 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1080 1742 2301
Size: 1080 662 559
Fragments arranged by size:
1080 662 559
BsrBI CCG'CTC
Cuts at: 0 1350 2301
Size: 1350 951
BsrFI r'CCGG_y
Cuts at: 0 338 799 2176 2301
Size: 338 461 1377 125
Fragments arranged by size:
1377 461 338 125
BsrGI T'GTAC_A
Cuts at: 0 194 1175 1565 2301
Size: 194 981 390 736
Fragments arranged by size:
981 736 390 194
BssSI C'ACGA_G
Cuts at: 0 1330 2301
Size: 1330 971
BstEII G'GTnAC_C
Cuts at: 0 181 2301
Size: 181 2120
BstYI r'GATC_y
Cuts at: 0 554 1391 1544 1877 2093 2301
Size: 554 837 153 333 216 208
Fragments arranged by size:
837 554 333 216 208 153
Bsu36I CC'TnA_GG
Cuts at: 0 2245 2301
Size: 2245 56
BtgI C'CryG_G
Cuts at: 0 604 747 1014 1290 1985 2162 2301
Size: 604 143 267 276 695 177 139
Fragments arranged by size:
695 604 276 267 177 143 139
BtsI GCAGTG_nn'
Cuts at: 0 1057 2301
Size: 1057 1244
DrdI GACnn_nn'nnGTC
Cuts at: 0 1090 2301
Size: 1090 1211
EaeI y'GGCC_r
Cuts at: 0 737 960 1622 1937 1946 1982 2018 2178
Size: 737 223 662 315 9 36 36 160
Cuts at: 2178 2301
Size: 123
Fragments arranged by size:
737 662 315 223 160 123 36 36
9
EarI CTCTTCn'nnn_
Cuts at: 0 1819 2301
Size: 1819 482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 1117 1185 2068 2301
Size: 916 201 68 883 233
Fragments arranged by size:
916 883 233 201 68
EcoICRI GAG'CTC
Cuts at: 0 305 353 2040 2301
Size: 305 48 1687 261
Fragments arranged by size:
1687 305 261 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 895 921 2301
Size: 895 26 1380
Fragments arranged by size:
1380 895 26
EcoO109I rG'GnC_Cy
Cuts at: 0 26 83 243 279 1629 2262 2301
Size: 26 57 160 36 1350 633 39
Fragments arranged by size:
1350 633 160 57 39 36 26
HaeII r_GCGC'y
Cuts at: 0 736 1360 1615 1873 2301
Size: 736 624 255 258 428
Fragments arranged by size:
736 624 428 258 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 555 587 670 682 702 714
Size: 95 32 428 32 83 12 20 12
Cuts at: 714 1018 1050 1324 1356 2137 2169 2301
Size: 304 32 274 32 781 32 132
Fragments arranged by size:
781 428 304 274 132 95 83 32
32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 563 2301
Size: 563 1738
KasI G'GCGC_C
Cuts at: 0 1611 2301
Size: 1611 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1322 1580 2301
Size: 818 504 258 721
Fragments arranged by size:
818 721 504 258
MscI TGG'CCA
Cuts at: 0 1939 1948 1984 2020 2301
Size: 1939 9 36 36 281
Fragments arranged by size:
1939 281 36 36 9
MslI CAynn'nnrTG
Cuts at: 0 424 1069 1214 1933 2026 2301
Size: 424 645 145 719 93 275
Fragments arranged by size:
719 645 424 275 145 93
MspA1I CmG'CkG
Cuts at: 0 74 80 606 749 861 959 966 1292
Size: 74 6 526 143 112 98 7 326
Cuts at: 1292 1826 1980 2150 2164 2301
Size: 534 154 170 14 137
Fragments arranged by size:
534 526 326 170 154 143 137 112
98 74 14 7 6
NaeI GCC'GGC
Cuts at: 0 340 801 2301
Size: 340 461 1500
Fragments arranged by size:
1500 461 340
NarI GG'CG_CC
Cuts at: 0 1612 2301
Size: 1612 689
NgoMIV G'CCGG_C
Cuts at: 0 338 799 2301
Size: 338 461 1502
Fragments arranged by size:
1502 461 338
NsiI A_TGCA'T
Cuts at: 0 1114 2301
Size: 1114 1187
NspI r_CATG'y
Cuts at: 0 456 918 1112 1116 1176 2142 2301
Size: 456 462 194 4 60 966 159
Fragments arranged by size:
966 462 456 194 159 60 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 1779 2301
Size: 1779 522
PfoI T'CCnGG_A
Cuts at: 0 95 550 812 1631 2301
Size: 95 455 262 819 670
Fragments arranged by size:
819 670 455 262 95
PmlI CAC'GTG
Cuts at: 0 1761 2301
Size: 1761 540
PpuMI rG'GwC_Cy
Cuts at: 0 83 243 1629 2301
Size: 83 160 1386 672
Fragments arranged by size:
1386 672 160 83
PspOMI G'GGCC_C
Cuts at: 0 1895 2262 2301
Size: 1895 367 39
Fragments arranged by size:
1895 367 39
PstI C_TGCA'G
Cuts at: 0 158 836 1370 1808 2104 2301
Size: 158 678 534 438 296 197
Fragments arranged by size:
678 534 438 296 197 158
PvuII CAG'CTG
Cuts at: 0 966 1980 2301
Size: 966 1014 321
Fragments arranged by size:
1014 966 321
SacI G_AGCT'C
Cuts at: 0 307 355 2042 2301
Size: 307 48 1687 259
Fragments arranged by size:
1687 307 259 48
SacII CC_GC'GG
Cuts at: 0 607 750 1293 2165 2301
Size: 607 143 543 872 136
Fragments arranged by size:
872 607 543 143 136
SalI G'TCGA_C
Cuts at: 0 561 2301
Size: 561 1740
SanDI GG'GwC_CC
Cuts at: 0 83 243 2301
Size: 83 160 2058
Fragments arranged by size:
2058 160 83
SapI GCTCTTCn'nnn_
Cuts at: 0 1819 2301
Size: 1819 482
ScaI AGT'ACT
Cuts at: 0 1384 2301
Size: 1384 917
SexAI A'CCwGG_T
Cuts at: 0 1604 1778 2301
Size: 1604 174 523
Fragments arranged by size:
1604 523 174
SfcI C'TryA_G
Cuts at: 0 154 832 1366 1804 2100 2301
Size: 154 678 534 438 296 201
Fragments arranged by size:
678 534 438 296 201 154
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1894 1945 2301
Size: 1894 51 356
Fragments arranged by size:
1894 356 51
SfoI GGC'GCC
Cuts at: 0 1613 2301
Size: 1613 688
SmaI CCC'GGG
Cuts at: 0 1702 2301
Size: 1702 599
SphI G_CATG'C
Cuts at: 0 456 1116 2301
Size: 456 660 1185
Fragments arranged by size:
1185 660 456
StuI AGG'CCT
Cuts at: 0 949 1888 2301
Size: 949 939 413
Fragments arranged by size:
949 939 413
StyI C'CwwG_G
Cuts at: 0 1590 2301
Size: 1590 711
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 191 2301
Size: 191 2110
TatI w'GTAC_w
Cuts at: 0 194 992 1175 1241 1382 1565 2301
Size: 194 798 183 66 141 183 736
Fragments arranged by size:
798 736 194 183 183 141 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 904 936 2301
Size: 358 32 514 32 1365
Fragments arranged by size:
1365 514 358 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1474 2301
Size: 1474 827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 963 2163 2301
Size: 231 732 1200 138
Fragments arranged by size:
1200 732 231 138
XmaI C'CCGG_G
Cuts at: 0 1700 2301
Size: 1700 601
ZraI GAC'GTC
Cuts at: 0 1965 2301
Size: 1965 336
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BfrBI BglI Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssSI BstEII BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SalI SanDI SapI ScaI
SexAI SfcI SfiI SfoI SmaI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BbvCI BciVI BclI BglII
BlpI BmrI BmtI BpuEI BsaBI BsiEI BsiWI BsmI
BspHI BsrDI BssHII BstAPI BstBI BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SbfI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmnI