Restriction map for
pFN21AE4219
(Linear) (Six-base) MAPSORT of: KIEE4219.seq Check: 6541 from: 1 to: 2154
KIEE4219.orf
With 182 enzymes: SgfI *
July 3, 2012 18:47 ..
AccI GT'mk_AC
Cuts at: 0 98 2154
Size: 98 2056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 1764 2154
Size: 1501 263 390
Fragments arranged by size:
1501 390 263
AhdI GACnn_n'nnGTC
Cuts at: 0 504 2154
Size: 504 1650
AleI CACnn'nnGTG
Cuts at: 0 451 2154
Size: 451 1703
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 2154
Size: 223 1931
AlwNI CAG_nnn'CTG
Cuts at: 0 27 294 2154
Size: 27 267 1860
Fragments arranged by size:
1860 267 27
ApaI G_GGCC'C
Cuts at: 0 109 136 2154
Size: 109 27 2018
Fragments arranged by size:
2018 109 27
ApoI r'AATT_y
Cuts at: 0 1462 2154
Size: 1462 692
AvaI C'yCGr_G
Cuts at: 0 236 259 2154
Size: 236 23 1895
Fragments arranged by size:
1895 236 23
BanI G'GyrC_C
Cuts at: 0 363 369 400 2154
Size: 363 6 31 1754
Fragments arranged by size:
1754 363 31 6
BanII G_rGCy'C
Cuts at: 0 109 136 636 950 1454 1877 2067 2154
Size: 109 27 500 314 504 423 190 87
Fragments arranged by size:
504 500 423 314 190 109 87 27
BbeI G_GCGC'C
Cuts at: 0 367 373 2154
Size: 367 6 1781
Fragments arranged by size:
1781 367 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1847 2154
Size: 1847 307
BciVI GTATCCnnnnn_n'
Cuts at: 0 1946 2154
Size: 1946 208
BclI T'GATC_A
Cuts at: 0 1212 2154
Size: 1212 942
BfrBI ATG'CAT
Cuts at: 0 1634 2154
Size: 1634 520
BglI GCCn_nnn'nGGC
Cuts at: 0 433 640 698 2154
Size: 433 207 58 1456
Fragments arranged by size:
1456 433 207 58
BlpI GC'TnA_GC
Cuts at: 0 28 302 685 1871 2066 2154
Size: 28 274 383 1186 195 88
Fragments arranged by size:
1186 383 274 195 88 28
Bme1580I G_kGCm'C
Cuts at: 0 109 136 2154
Size: 109 27 2018
Fragments arranged by size:
2018 109 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 2154
Size: 626 1528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2086 2154
Size: 2086 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 964 2154
Size: 331 633 1190
Fragments arranged by size:
1190 633 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 2154
Size: 775 1379
BsaI GGTCTCn'nnnn_
Cuts at: 0 854 2154
Size: 854 1300
BsaHI Gr'CG_yC
Cuts at: 0 224 232 364 370 1988 2154
Size: 224 8 132 6 1618 166
Fragments arranged by size:
1618 224 166 132 8 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1060 1090 1664 1694 2154
Size: 1060 30 574 30 460
Fragments arranged by size:
1060 574 460 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1081 1084 1462 2154
Size: 685 396 3 378 692
Fragments arranged by size:
692 685 396 378 3
BseYI C'CCAG_C
Cuts at: 0 643 717 1011 2016 2154
Size: 643 74 294 1005 138
Fragments arranged by size:
1005 643 294 138 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1576 2154
Size: 1576 578
BsiEI CG_ry'CG
Cuts at: 0 567 2154
Size: 567 1587
BsiHKAI G_wGCw'C
Cuts at: 0 686 950 1454 1790 1877 2067 2154
Size: 686 264 504 336 87 190 87
Fragments arranged by size:
686 504 336 264 190 87 87
BsmI GAATG_Cn'
Cuts at: 0 888 1279 2154
Size: 888 391 875
Fragments arranged by size:
888 875 391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 2154
Size: 412 1742
Bsp1286I G_dGCh'C
Cuts at: 0 109 136 636 686 950 1454 1790 1877
Size: 109 27 500 50 264 504 336 87
Cuts at: 1877 2067 2154
Size: 190 87
Fragments arranged by size:
504 500 336 264 190 109 87 87
50 27
BspHI T'CATG_A
Cuts at: 0 876 2154
Size: 876 1278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1236 2154
Size: 1236 918
BsrBI CCG'CTC
Cuts at: 0 285 1748 2154
Size: 285 1463 406
Fragments arranged by size:
1463 406 285
BsrDI GCAATG_nn'
Cuts at: 0 1143 2090 2154
Size: 1143 947 64
Fragments arranged by size:
1143 947 64
BsrFI r'CCGG_y
Cuts at: 0 366 566 2154
Size: 366 200 1588
Fragments arranged by size:
1588 366 200
BssSI C'ACGA_G
Cuts at: 0 742 1647 2154
Size: 742 905 507
Fragments arranged by size:
905 742 507
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 2154
Size: 321 1833
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1807 2154
Size: 1807 347
BstYI r'GATC_y
Cuts at: 0 1200 1674 2154
Size: 1200 474 480
Fragments arranged by size:
1200 480 474
BtgI C'CryG_G
Cuts at: 0 404 431 2154
Size: 404 27 1723
Fragments arranged by size:
1723 404 27
BtsI GCAGTG_nn'
Cuts at: 0 389 784 886 2154
Size: 389 395 102 1268
Fragments arranged by size:
1268 395 389 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 511 546 2154
Size: 511 35 1608
Fragments arranged by size:
1608 511 35
EaeI y'GGCC_r
Cuts at: 0 443 519 611 1743 2154
Size: 443 76 92 1132 411
Fragments arranged by size:
1132 443 411 92 76
EarI CTCTTCn'nnn_
Cuts at: 0 410 1058 1499 2154
Size: 410 648 441 655
Fragments arranged by size:
655 648 441 410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 349 427 588 907 2154
Size: 85 264 78 161 319 1247
Fragments arranged by size:
1247 319 264 161 85 78
EcoICRI GAG'CTC
Cuts at: 0 948 1452 1875 2065 2154
Size: 948 504 423 190 89
Fragments arranged by size:
948 504 423 190 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 964 1501 1764 2154
Size: 331 633 537 263 390
Fragments arranged by size:
633 537 390 331 263
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1682 2154
Size: 1682 472
EcoO109I rG'GnC_Cy
Cuts at: 0 106 132 133 182 2090 2154
Size: 106 26 1 49 1908 64
Fragments arranged by size:
1908 106 64 49 26 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1414 1794 2154
Size: 1414 380 360
Fragments arranged by size:
1414 380 360
HaeII r_GCGC'y
Cuts at: 0 361 367 373 573 771 1779 2154
Size: 361 6 6 200 198 1008 375
Fragments arranged by size:
1008 375 361 200 198 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 1072 1104 1664 1696 1732 1764
Size: 490 32 550 32 560 32 36 32
Cuts at: 1764 1944 1976 2154
Size: 180 32 178
Fragments arranged by size:
560 550 490 180 178 36 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 675 1758 2154
Size: 675 1083 396
Fragments arranged by size:
1083 675 396
HindIII A'AGCT_T
Cuts at: 0 1495 2154
Size: 1495 659
KasI G'GCGC_C
Cuts at: 0 363 369 2154
Size: 363 6 1785
Fragments arranged by size:
1785 363 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 2154
Size: 1058 1096
MscI TGG'CCA
Cuts at: 0 521 613 2154
Size: 521 92 1541
Fragments arranged by size:
1541 521 92
MslI CAynn'nnrTG
Cuts at: 0 451 881 1892 2154
Size: 451 430 1011 262
Fragments arranged by size:
1011 451 430 262
MspA1I CmG'CkG
Cuts at: 0 88 294 309 318 433 666 689 1803
Size: 88 206 15 9 115 233 23 1114
Cuts at: 1803 2154
Size: 351
Fragments arranged by size:
1114 351 233 206 115 88 23 15
9
NaeI GCC'GGC
Cuts at: 0 368 2154
Size: 368 1786
NarI GG'CG_CC
Cuts at: 0 364 370 2154
Size: 364 6 1784
Fragments arranged by size:
1784 364 6
NgoMIV G'CCGG_C
Cuts at: 0 366 2154
Size: 366 1788
NsiI A_TGCA'T
Cuts at: 0 1636 2154
Size: 1636 518
NspI r_CATG'y
Cuts at: 0 911 1665 2154
Size: 911 754 489
Fragments arranged by size:
911 754 489
PpuMI rG'GwC_Cy
Cuts at: 0 182 2090 2154
Size: 182 1908 64
Fragments arranged by size:
1908 182 64
PspOMI G'GGCC_C
Cuts at: 0 105 132 2154
Size: 105 27 2022
Fragments arranged by size:
2022 105 27
PspXI vC'TCGA_Gb
Cuts at: 0 259 2154
Size: 259 1895
PstI C_TGCA'G
Cuts at: 0 129 2154
Size: 129 2025
PvuII CAG'CTG
Cuts at: 0 294 309 318 2154
Size: 294 15 9 1836
Fragments arranged by size:
1836 294 15 9
SacI G_AGCT'C
Cuts at: 0 950 1454 1877 2067 2154
Size: 950 504 423 190 87
Fragments arranged by size:
950 504 423 190 87
SacII CC_GC'GG
Cuts at: 0 434 2154
Size: 434 1720
SfcI C'TryA_G
Cuts at: 0 125 2154
Size: 125 2029
SfoI GGC'GCC
Cuts at: 0 365 371 2154
Size: 365 6 1783
Fragments arranged by size:
1783 365 6
SgrAI Cr'CCGG_yG
Cuts at: 0 366 2154
Size: 366 1788
SmaI CCC'GGG
Cuts at: 0 238 2154
Size: 238 1916
SmlI C'TyrA_G
Cuts at: 0 259 790 2154
Size: 259 531 1364
Fragments arranged by size:
1364 531 259
StyI C'CwwG_G
Cuts at: 0 227 866 1668 2051 2154
Size: 227 639 802 383 103
Fragments arranged by size:
802 639 383 227 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 128 836 2154
Size: 128 708 1318
Fragments arranged by size:
1318 708 128
TatI w'GTAC_w
Cuts at: 0 794 1558 2154
Size: 794 764 596
Fragments arranged by size:
794 764 596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2024 2154
Size: 2024 130
XhoI C'TCGA_G
Cuts at: 0 259 2154
Size: 259 1895
XmaI C'CCGG_G
Cuts at: 0 236 2154
Size: 236 1918
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BtgI BtsI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SfcI SfoI SgrAI SmaI SmlI
StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BglII BmgBI BmtI Bpu10I BsaAI BsaBI
BsaWI BsiWI BspEI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XmnI ZraI