Restriction map for
pFN21AE4214
(Linear) (Six-base) MAPSORT of: KIEE4214.seq Check: 7827 from: 1 to: 2205
KIEE4214.orf
With 182 enzymes: SgfI *
March 22, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1613 1883 2205
Size: 1613 270 322
Fragments arranged by size:
1613 322 270
Acc65I G'GTAC_C
Cuts at: 0 297 2205
Size: 297 1908
AflIII A'CryG_T
Cuts at: 0 2195 2205
Size: 2195 10
AgeI A'CCGG_T
Cuts at: 0 2086 2205
Size: 2086 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1389 2205
Size: 1069 320 816
Fragments arranged by size:
1069 816 320
AlwNI CAG_nnn'CTG
Cuts at: 0 373 655 1119 1470 2205
Size: 373 282 464 351 735
Fragments arranged by size:
735 464 373 351 282
ApaI G_GGCC'C
Cuts at: 0 173 484 637 725 903 2205
Size: 173 311 153 88 178 1302
Fragments arranged by size:
1302 311 178 173 153 88
AvaI C'yCGr_G
Cuts at: 0 181 381 477 554 637 672 770 823
Size: 181 200 96 77 83 35 98 53
Cuts at: 823 1499 1541 1938 2205
Size: 676 42 397 267
Fragments arranged by size:
676 397 267 200 181 98 96 83
77 53 42 35
BanI G'GyrC_C
Cuts at: 0 297 491 530 661 774 1025 1045 1076
Size: 297 194 39 131 113 251 20 31
Cuts at: 1076 1236 1537 1698 2205
Size: 160 301 161 507
Fragments arranged by size:
507 301 297 251 194 161 160 131
113 39 31 20
BanII G_rGCy'C
Cuts at: 0 113 134 173 199 233 317 380 484
Size: 113 21 39 26 34 84 63 104
Cuts at: 484 595 637 673 725 903 1178 1550 2180
Size: 111 42 36 52 178 275 372 630
Cuts at: 2180 2205
Size: 25
Fragments arranged by size:
630 372 275 178 113 111 104 84
63 52 42 39 36 34 26 25
21
BbeI G_GCGC'C
Cuts at: 0 495 534 1702 2205
Size: 495 39 1168 503
Fragments arranged by size:
1168 503 495 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1059 2105 2205
Size: 359 700 1046 100
Fragments arranged by size:
1046 700 359 100
BbvCI CC'TCA_GC
Cuts at: 0 272 688 802 1552 2205
Size: 272 416 114 750 653
Fragments arranged by size:
750 653 416 272 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 596 2092 2126 2205
Size: 562 34 1496 34 79
Fragments arranged by size:
1496 562 79 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 41 384 548 1116 1707 1995 2205
Size: 41 343 164 568 591 288 210
Fragments arranged by size:
591 568 343 288 210 164 41
BglII A'GATC_T
Cuts at: 0 326 2205
Size: 326 1879
BlpI GC'TnA_GC
Cuts at: 0 399 1172 1317 2205
Size: 399 773 145 888
Fragments arranged by size:
888 773 399 145
Bme1580I G_kGCm'C
Cuts at: 0 173 484 637 664 725 777 903 1041
Size: 173 311 153 27 61 52 126 138
Cuts at: 1041 1048 1241 1540 1719 2205
Size: 7 193 299 179 486
Fragments arranged by size:
486 311 299 193 179 173 153 138
126 61 52 27 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 317 2205
Size: 227 90 1888
Fragments arranged by size:
1888 227 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 189 297 1567 2205
Size: 189 108 1270 638
Fragments arranged by size:
1270 638 189 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 159 247 350 982 988 1126 1507
Size: 104 55 88 103 632 6 138 381
Cuts at: 1507 1811 1983 2188 2205
Size: 304 172 205 17
Fragments arranged by size:
632 381 304 205 172 138 104 103
88 55 17 6
Bpu10I CC'TnA_GC
Cuts at: 0 272 374 538 688 802 1552 2205
Size: 272 102 164 150 114 750 653
Fragments arranged by size:
750 653 272 164 150 114 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1408 2205
Size: 1408 797
BsaI GGTCTCn'nnnn_
Cuts at: 0 826 2205
Size: 826 1379
BsaAI yAC'GTr
Cuts at: 0 2031 2205
Size: 2031 174
BsaHI Gr'CG_yC
Cuts at: 0 74 492 531 1699 2150 2205
Size: 74 418 39 1168 451 55
Fragments arranged by size:
1168 451 418 74 55 39
BsaWI w'CCGG_w
Cuts at: 0 705 2086 2205
Size: 705 1381 119
Fragments arranged by size:
1381 705 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1540 1608 1934 2077 2205
Size: 1540 68 326 143 128
Fragments arranged by size:
1540 326 143 128 68
BseYI C'CCAG_C
Cuts at: 0 93 98 112 252 714 718 1463 2205
Size: 93 5 14 140 462 4 745 742
Fragments arranged by size:
745 742 462 140 93 14 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 452 793 1385 1762 1766 1855 2205
Size: 185 267 341 592 377 4 89 350
Fragments arranged by size:
592 377 350 341 267 185 89 4
BsiEI CG_ry'CG
Cuts at: 0 785 1917 2205
Size: 785 1132 288
Fragments arranged by size:
1132 785 288
BsiHKAI G_wGCw'C
Cuts at: 0 2180 2205
Size: 2180 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1620 2205
Size: 1620 585
Bsp1286I G_dGCh'C
Cuts at: 0 113 134 173 199 233 317 380 484
Size: 113 21 39 26 34 84 63 104
Cuts at: 484 595 637 664 673 725 777 903 1041
Size: 111 42 27 9 52 52 126 138
Cuts at: 1041 1048 1178 1241 1540 1550 1719 2180 2205
Size: 7 130 63 299 10 169 461 25
Fragments arranged by size:
461 299 169 138 130 126 113 111
104 84 63 63 52 52 42 39
34 27 26 25 21 10 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1613 1793 1883 2205
Size: 224 1389 180 90 322
Fragments arranged by size:
1389 322 224 180 90
BsrBI CCG'CTC
Cuts at: 0 1215 1221 1356 2205
Size: 1215 6 135 849
Fragments arranged by size:
1215 849 135 6
BsrFI r'CCGG_y
Cuts at: 0 319 642 679 1028 1686 1954 2086 2205
Size: 319 323 37 349 658 268 132 119
Fragments arranged by size:
658 349 323 319 268 132 119 37
BssHII G'CGCG_C
Cuts at: 0 987 2205
Size: 987 1218
BssSI C'ACGA_G
Cuts at: 0 1712 2205
Size: 1712 493
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1785 2205
Size: 1785 420
BstBI TT'CG_AA
Cuts at: 0 2096 2205
Size: 2096 109
BstEII G'GTnAC_C
Cuts at: 0 682 2082 2205
Size: 682 1400 123
Fragments arranged by size:
1400 682 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1749 2205
Size: 1749 456
BstYI r'GATC_y
Cuts at: 0 326 1803 2163 2205
Size: 326 1477 360 42
Fragments arranged by size:
1477 360 326 42
Bsu36I CC'TnA_GG
Cuts at: 0 347 1112 2205
Size: 347 765 1093
Fragments arranged by size:
1093 765 347
BtgI C'CryG_G
Cuts at: 0 1061 1493 1911 2205
Size: 1061 432 418 294
Fragments arranged by size:
1061 432 418 294
BtsI GCAGTG_nn'
Cuts at: 0 913 1201 1331 1754 2046 2205
Size: 913 288 130 423 292 159
Fragments arranged by size:
913 423 292 288 159 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1637 1672 2205
Size: 1637 35 533
Fragments arranged by size:
1637 533 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1304 2065 2205
Size: 1304 761 140
Fragments arranged by size:
1304 761 140
EaeI y'GGCC_r
Cuts at: 0 2 87 782 835 1094 1230 1658 1798
Size: 2 85 695 53 259 136 428 140
Cuts at: 1798 2205
Size: 407
Fragments arranged by size:
695 428 407 259 140 136 85 53
2
EagI C'GGCC_G
Cuts at: 0 782 2205
Size: 782 1423
EcoICRI GAG'CTC
Cuts at: 0 2178 2205
Size: 2178 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 159 247 350 982 988 1126 1507
Size: 104 55 88 103 632 6 138 381
Cuts at: 1507 1811 1983 2188 2205
Size: 304 172 205 17
Fragments arranged by size:
632 381 304 205 172 138 104 103
88 55 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1381 1474 2205
Size: 1381 93 731
Fragments arranged by size:
1381 731 93
EcoO109I rG'GnC_Cy
Cuts at: 0 149 169 170 289 414 481 550 633
Size: 149 20 1 119 125 67 69 83
Cuts at: 633 634 652 722 760 861 899 1008 1386
Size: 1 18 70 38 101 38 109 378
Cuts at: 1386 1503 1924 2205
Size: 117 421 281
Fragments arranged by size:
421 378 281 149 125 119 117 109
101 83 70 69 67 38 38 20
18 1 1
EcoRV GAT'ATC
Cuts at: 0 1401 2205
Size: 1401 804
HaeII r_GCGC'y
Cuts at: 0 105 495 534 714 844 1278 1702 2205
Size: 105 390 39 180 130 434 424 503
Fragments arranged by size:
503 434 424 390 180 130 105 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 1993 2025 2205
Size: 685 32 1276 32 180
Fragments arranged by size:
1276 685 180 32 32
KasI G'GCGC_C
Cuts at: 0 491 530 1698 2205
Size: 491 39 1168 507
Fragments arranged by size:
1168 507 491 39
KpnI G_GTAC'C
Cuts at: 0 301 2205
Size: 301 1904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1124 2205
Size: 50 1074 1081
Fragments arranged by size:
1081 1074 50
MscI TGG'CCA
Cuts at: 0 1800 2205
Size: 1800 405
MslI CAynn'nnrTG
Cuts at: 0 505 1771 2205
Size: 505 1266 434
Fragments arranged by size:
1266 505 434
MspA1I CmG'CkG
Cuts at: 0 271 403 718 1092 1171 1321 1369 1556
Size: 271 132 315 374 79 150 48 187
Cuts at: 1556 1881 1913 2205
Size: 325 32 292
Fragments arranged by size:
374 325 315 292 271 187 150 132
79 48 32
NaeI GCC'GGC
Cuts at: 0 321 644 1688 1956 2205
Size: 321 323 1044 268 249
Fragments arranged by size:
1044 323 321 268 249
NarI GG'CG_CC
Cuts at: 0 492 531 1699 2205
Size: 492 39 1168 506
Fragments arranged by size:
1168 506 492 39
NcoI C'CATG_G
Cuts at: 0 1493 2205
Size: 1493 712
NgoMIV G'CCGG_C
Cuts at: 0 319 642 1686 1954 2205
Size: 319 323 1044 268 251
Fragments arranged by size:
1044 323 319 268 251
PasI CC'CwG_GG
Cuts at: 0 857 1041 2205
Size: 857 184 1164
Fragments arranged by size:
1164 857 184
PflMI CCAn_nnn'nTGG
Cuts at: 0 394 1092 2205
Size: 394 698 1113
Fragments arranged by size:
1113 698 394
PfoI T'CCnGG_A
Cuts at: 0 1595 2159 2205
Size: 1595 564 46
Fragments arranged by size:
1595 564 46
PmlI CAC'GTG
Cuts at: 0 2031 2205
Size: 2031 174
PpuMI rG'GwC_Cy
Cuts at: 0 149 760 861 1008 1386 1503 2205
Size: 149 611 101 147 378 117 702
Fragments arranged by size:
702 611 378 149 147 117 101
PspOMI G'GGCC_C
Cuts at: 0 169 480 633 721 899 2205
Size: 169 311 153 88 178 1306
Fragments arranged by size:
1306 311 178 169 153 88
PstI C_TGCA'G
Cuts at: 0 1793 2205
Size: 1793 412
PvuII CAG'CTG
Cuts at: 0 271 403 718 1092 1171 1556 2205
Size: 271 132 315 374 79 385 649
Fragments arranged by size:
649 385 374 315 271 132 79
SacI G_AGCT'C
Cuts at: 0 2180 2205
Size: 2180 25
SacII CC_GC'GG
Cuts at: 0 1914 2205
Size: 1914 291
SanDI GG'GwC_CC
Cuts at: 0 149 760 1008 1503 2205
Size: 149 611 248 495 702
Fragments arranged by size:
702 611 495 248 149
SfcI C'TryA_G
Cuts at: 0 1789 2205
Size: 1789 416
SfoI GGC'GCC
Cuts at: 0 493 532 1700 2205
Size: 493 39 1168 505
Fragments arranged by size:
1168 505 493 39
SgrAI Cr'CCGG_yG
Cuts at: 0 319 1954 2086 2205
Size: 319 1635 132 119
Fragments arranged by size:
1635 319 132 119
SmaI CCC'GGG
Cuts at: 0 383 479 556 639 772 1501 2205
Size: 383 96 77 83 133 729 704
Fragments arranged by size:
729 704 383 133 96 83 77
SmlI C'TyrA_G
Cuts at: 0 1423 2205
Size: 1423 782
SrfI GCCC'GGGC
Cuts at: 0 772 2205
Size: 772 1433
StuI AGG'CCT
Cuts at: 0 1710 1736 2205
Size: 1710 26 469
Fragments arranged by size:
1710 469 26
StyI C'CwwG_G
Cuts at: 0 77 426 1493 2205
Size: 77 349 1067 712
Fragments arranged by size:
1067 712 349 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 237 244 747 1477 2205
Size: 107 130 7 503 730 728
Fragments arranged by size:
730 728 503 130 107 7
Tth111I GACn'n_nGTC
Cuts at: 0 1140 2205
Size: 1140 1065
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1258 2205
Size: 1258 947
XmaI C'CCGG_G
Cuts at: 0 381 477 554 637 770 1499 2205
Size: 381 96 77 83 133 729 706
Fragments arranged by size:
729 706 381 133 96 83 77
XmnI GAAnn'nnTTC
Cuts at: 0 25 2205
Size: 25 2180
Enzymes that do cut:
AarI Acc65I AflIII AgeI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI
SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BmgBI BmtI BsaBI BsaXI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BstZ17I BtgZI
ClaI DraI DraIII EarI EciI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SspI SwaI TatI TstI XbaI XhoI ZraI