Restriction map for
pFN21AE4210
(Linear) (Six-base) MAPSORT of: KIEE4210.seq Check: 4287 from: 1 to: 2190
KIEE4210.orf
With 182 enzymes: SgfI *
March 22, 2012 17:35 ..
AccI GT'mk_AC
Cuts at: 0 2027 2190
Size: 2027 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 625 2190
Size: 91 534 1565
Fragments arranged by size:
1565 534 91
AlwNI CAG_nnn'CTG
Cuts at: 0 90 389 816 2190
Size: 90 299 427 1374
Fragments arranged by size:
1374 427 299 90
ApaI G_GGCC'C
Cuts at: 0 1276 1438 2190
Size: 1276 162 752
Fragments arranged by size:
1276 752 162
ApoI r'AATT_y
Cuts at: 0 781 1279 2143 2190
Size: 781 498 864 47
Fragments arranged by size:
864 781 498 47
AvaI C'yCGr_G
Cuts at: 0 442 1689 2040 2127 2190
Size: 442 1247 351 87 63
Fragments arranged by size:
1247 442 351 87 63
BamHI G'GATC_C
Cuts at: 0 623 2190
Size: 623 1567
BanI G'GyrC_C
Cuts at: 0 147 361 2190
Size: 147 214 1829
Fragments arranged by size:
1829 214 147
BanII G_rGCy'C
Cuts at: 0 853 1276 1403 1438 1697 1720 2056 2190
Size: 853 423 127 35 259 23 336 134
Fragments arranged by size:
853 423 336 259 134 127 35 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 613 2190
Size: 613 1577
BbvCI CC'TCA_GC
Cuts at: 0 415 1579 2190
Size: 415 1164 611
Fragments arranged by size:
1164 611 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 879 913 2190
Size: 879 34 1277
Fragments arranged by size:
1277 879 34
BlpI GC'TnA_GC
Cuts at: 0 2161 2190
Size: 2161 29
Bme1580I G_kGCm'C
Cuts at: 0 364 1229 1276 1438 1746 2190
Size: 364 865 47 162 308 444
Fragments arranged by size:
865 444 364 308 162 47
BmgBI CAC'GTC
Cuts at: 0 933 2190
Size: 933 1257
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 1244 1441 2190
Size: 647 597 197 749
Fragments arranged by size:
749 647 597 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 838 1030 1375 1501 1619 1823 1899
Size: 208 630 192 345 126 118 204 76
Cuts at: 1899 2190
Size: 291
Fragments arranged by size:
630 345 291 208 204 192 126 118
76
Bpu10I CC'TnA_GC
Cuts at: 0 415 1579 2190
Size: 415 1164 611
Fragments arranged by size:
1164 611 415
BsaI GGTCTCn'nnnn_
Cuts at: 0 1162 1177 1206 1519 1810 2190
Size: 1162 15 29 313 291 380
Fragments arranged by size:
1162 380 313 291 29 15
BsaHI Gr'CG_yC
Cuts at: 0 1034 2190
Size: 1034 1156
BsaWI w'CCGG_w
Cuts at: 0 470 1361 2190
Size: 470 891 829
Fragments arranged by size:
891 829 470
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 135 165 979 1009 1435 1465 1795 1825
Size: 135 30 814 30 426 30 330 30
Cuts at: 1825 2190
Size: 365
Fragments arranged by size:
814 426 365 330 135 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 128 409 412 835 838 859 935 1456
Size: 128 281 3 423 3 21 76 521
Cuts at: 1456 1785 1899 1902 1978 2190
Size: 329 114 3 76 212
Fragments arranged by size:
521 423 329 281 212 128 114 76
76 21 3 3 3
BseYI C'CCAG_C
Cuts at: 0 265 375 852 879 1037 1425 1831 2056
Size: 265 110 477 27 158 388 406 225
Cuts at: 2056 2190
Size: 134
Fragments arranged by size:
477 406 388 265 225 158 134 110
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1693 2190
Size: 1180 513 497
Fragments arranged by size:
1180 513 497
BsiEI CG_ry'CG
Cuts at: 0 127 1893 2190
Size: 127 1766 297
Fragments arranged by size:
1766 297 127
BsiHKAI G_wGCw'C
Cuts at: 0 1403 2190
Size: 1403 787
BsiWI C'GTAC_G
Cuts at: 0 1506 2190
Size: 1506 684
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1025 2190
Size: 1025 1165
Bsp1286I G_dGCh'C
Cuts at: 0 364 853 1229 1276 1403 1438 1697 1720
Size: 364 489 376 47 127 35 259 23
Cuts at: 1720 1746 2056 2190
Size: 26 310 134
Fragments arranged by size:
489 376 364 310 259 134 127 47
35 26 23
BspHI T'CATG_A
Cuts at: 0 1871 2087 2190
Size: 1871 216 103
Fragments arranged by size:
1871 216 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 884 1152 2190
Size: 884 268 1038
Fragments arranged by size:
1038 884 268
BsrBI CCG'CTC
Cuts at: 0 129 621 1907 2190
Size: 129 492 1286 283
Fragments arranged by size:
1286 492 283 129
BsrFI r'CCGG_y
Cuts at: 0 122 514 2190
Size: 122 392 1676
Fragments arranged by size:
1676 392 122
BsrGI T'GTAC_A
Cuts at: 0 965 2190
Size: 965 1225
BstEII G'GTnAC_C
Cuts at: 0 1611 2190
Size: 1611 579
BstYI r'GATC_y
Cuts at: 0 623 2190
Size: 623 1567
Bsu36I CC'TnA_GG
Cuts at: 0 305 592 2190
Size: 305 287 1598
Fragments arranged by size:
1598 305 287
BtgI C'CryG_G
Cuts at: 0 939 1467 1520 1529 1958 2190
Size: 939 528 53 9 429 232
Fragments arranged by size:
939 528 429 232 53 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 356 2190
Size: 356 1834
BtsI GCAGTG_nn'
Cuts at: 0 24 698 895 1208 1937 2190
Size: 24 674 197 313 729 253
Fragments arranged by size:
729 674 313 253 197 24
DraIII CAC_nnn'GTG
Cuts at: 0 1750 2190
Size: 1750 440
EaeI y'GGCC_r
Cuts at: 0 6 92 124 1079 1539 1890 2190
Size: 6 86 32 955 460 351 300
Fragments arranged by size:
955 460 351 300 86 32 6
EagI C'GGCC_G
Cuts at: 0 124 1890 2190
Size: 124 1766 300
Fragments arranged by size:
1766 300 124
EarI CTCTTCn'nnn_
Cuts at: 0 209 398 1702 2190
Size: 209 189 1304 488
Fragments arranged by size:
1304 488 209 189
EcoICRI GAG'CTC
Cuts at: 0 1401 2190
Size: 1401 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 208 625 838 1030 1375 1501 1619
Size: 91 117 417 213 192 345 126 118
Cuts at: 1619 1823 1899 2190
Size: 204 76 291
Fragments arranged by size:
417 345 291 213 204 192 126 118
117 91 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 471 487 820 2190
Size: 471 16 333 1370
Fragments arranged by size:
1370 471 333 16
EcoO109I rG'GnC_Cy
Cuts at: 0 229 345 464 1272 2190
Size: 229 116 119 808 918
Fragments arranged by size:
918 808 229 119 116
EcoRI G'AATT_C
Cuts at: 0 1279 2190
Size: 1279 911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 885 1641 2190
Size: 885 756 549
Fragments arranged by size:
885 756 549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 693 725 1174 1206 1744 1776 1795 1807
Size: 693 32 449 32 538 32 19 12
Cuts at: 1807 1827 1839 2082 2114 2190
Size: 20 12 243 32 76
Fragments arranged by size:
693 538 449 243 76 32 32 32
32 20 19 12 12
HincII GTy'rAC
Cuts at: 0 768 2190
Size: 768 1422
HindIII A'AGCT_T
Cuts at: 0 208 646 2190
Size: 208 438 1544
Fragments arranged by size:
1544 438 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1316 2190
Size: 149 1167 874
Fragments arranged by size:
1167 874 149
MscI TGG'CCA
Cuts at: 0 94 1081 2190
Size: 94 987 1109
Fragments arranged by size:
1109 987 94
MslI CAynn'nnrTG
Cuts at: 0 1156 2190
Size: 1156 1034
MspA1I CmG'CkG
Cuts at: 0 1583 2071 2190
Size: 1583 488 119
Fragments arranged by size:
1583 488 119
NcoI C'CATG_G
Cuts at: 0 1520 1529 1958 2190
Size: 1520 9 429 232
Fragments arranged by size:
1520 429 232 9
NspI r_CATG'y
Cuts at: 0 1147 2190
Size: 1147 1043
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 1521 1786 2190
Size: 325 1196 265 404
Fragments arranged by size:
1196 404 325 265
PfoI T'CCnGG_A
Cuts at: 0 977 1379 1478 2190
Size: 977 402 99 712
Fragments arranged by size:
977 712 402 99
PpuMI rG'GwC_Cy
Cuts at: 0 229 345 2190
Size: 229 116 1845
Fragments arranged by size:
1845 229 116
PspOMI G'GGCC_C
Cuts at: 0 1272 1434 2190
Size: 1272 162 756
Fragments arranged by size:
1272 756 162
PspXI vC'TCGA_Gb
Cuts at: 0 442 2190
Size: 442 1748
PstI C_TGCA'G
Cuts at: 0 902 1944 2190
Size: 902 1042 246
Fragments arranged by size:
1042 902 246
PvuII CAG'CTG
Cuts at: 0 2071 2190
Size: 2071 119
SacI G_AGCT'C
Cuts at: 0 1403 2190
Size: 1403 787
SanDI GG'GwC_CC
Cuts at: 0 229 2190
Size: 229 1961
SapI GCTCTTCn'nnn_
Cuts at: 0 209 1702 2190
Size: 209 1493 488
Fragments arranged by size:
1493 488 209
SfcI C'TryA_G
Cuts at: 0 898 1940 2028 2190
Size: 898 1042 88 162
Fragments arranged by size:
1042 898 162 88
SmlI C'TyrA_G
Cuts at: 0 442 2190
Size: 442 1748
StuI AGG'CCT
Cuts at: 0 730 926 2190
Size: 730 196 1264
Fragments arranged by size:
1264 730 196
StyI C'CwwG_G
Cuts at: 0 629 725 1520 1529 1958 2190
Size: 629 96 795 9 429 232
Fragments arranged by size:
795 629 429 232 96 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1785 2190
Size: 1785 405
TatI w'GTAC_w
Cuts at: 0 965 2190
Size: 965 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3 35 135 167 1221 1253 2190
Size: 3 32 100 32 1054 32 937
Fragments arranged by size:
1054 937 100 32 32 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 813 1428 1527 2173 2190
Size: 813 615 99 646 17
Fragments arranged by size:
813 646 615 99 17
XhoI C'TCGA_G
Cuts at: 0 442 2190
Size: 442 1748
XmnI GAAnn'nnTTC
Cuts at: 0 1867 2190
Size: 1867 323
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrGI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NspI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI SapI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BclI BfrBI BglI BglII
BmtI BplI BpuEI BsaAI BsaBI BsmI BspEI BsrDI
BssHII BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI
DraI DrdI EciI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmaI ZraI