Restriction map for
pFN21AE4205
(Linear) (Six-base) MAPSORT of: KIEE4205.seq Check: 6381 from: 1 to: 2169
KIEE4205.orf
With 182 enzymes: SgfI *
March 22, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 121 766 2169
Size: 121 645 1403
Fragments arranged by size:
1403 645 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1144 1321 1608 2155 2169
Size: 996 148 177 287 547 14
Fragments arranged by size:
996 547 287 177 148 14
AfeI AGC'GCT
Cuts at: 0 649 748 2169
Size: 649 99 1421
Fragments arranged by size:
1421 649 99
AflIII A'CryG_T
Cuts at: 0 1870 2024 2169
Size: 1870 154 145
Fragments arranged by size:
1870 154 145
AhdI GACnn_n'nnGTC
Cuts at: 0 1743 2169
Size: 1743 426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 622 2169
Size: 469 153 1547
Fragments arranged by size:
1547 469 153
AlwNI CAG_nnn'CTG
Cuts at: 0 1836 2169
Size: 1836 333
ApaI G_GGCC'C
Cuts at: 0 704 1481 2169
Size: 704 777 688
Fragments arranged by size:
777 704 688
ApaLI G'TGCA_C
Cuts at: 0 1528 2169
Size: 1528 641
ApoI r'AATT_y
Cuts at: 0 1069 1906 1964 2169
Size: 1069 837 58 205
Fragments arranged by size:
1069 837 205 58
AvaI C'yCGr_G
Cuts at: 0 616 2169
Size: 616 1553
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 365 398 2169
Size: 365 33 1771
Fragments arranged by size:
1771 365 33
BanII G_rGCy'C
Cuts at: 0 181 704 1466 1481 1677 1955 2169
Size: 181 523 762 15 196 278 214
Fragments arranged by size:
762 523 278 214 196 181 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1810 1889 2169
Size: 1810 79 280
Fragments arranged by size:
1810 280 79
BglII A'GATC_T
Cuts at: 0 863 2169
Size: 863 1306
Bme1580I G_kGCm'C
Cuts at: 0 704 1427 1481 1532 2169
Size: 704 723 54 51 637
Fragments arranged by size:
723 704 637 54 51
BmgBI CAC'GTC
Cuts at: 0 825 1887 2169
Size: 825 1062 282
Fragments arranged by size:
1062 825 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 371 559 1671 2169
Size: 371 188 1112 498
Fragments arranged by size:
1112 498 371 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1678 1743 1751 2011 2140 2169
Size: 1678 65 8 260 129 29
Fragments arranged by size:
1678 260 129 65 29 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 772 1126 2169
Size: 658 114 354 1043
Fragments arranged by size:
1043 658 354 114
BsaAI yAC'GTr
Cuts at: 0 1422 2025 2169
Size: 1422 603 144
Fragments arranged by size:
1422 603 144
BsaBI GATnn'nnATC
Cuts at: 0 868 2169
Size: 868 1301
BsaHI Gr'CG_yC
Cuts at: 0 636 983 2169
Size: 636 347 1186
Fragments arranged by size:
1186 636 347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 973 1003 1914 1944 2169
Size: 679 30 264 30 911 30 225
Fragments arranged by size:
911 679 264 225 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 684 1485 1599 1919 2169
Size: 330 354 801 114 320 250
Fragments arranged by size:
801 354 330 320 250 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 147 222 445 465 613 912 1390
Size: 93 54 75 223 20 148 299 478
Cuts at: 1390 1981 2169
Size: 591 188
Fragments arranged by size:
591 478 299 223 188 148 93 75
54 20
BsiEI CG_ry'CG
Cuts at: 0 576 2169
Size: 576 1593
BsiHKAI G_wGCw'C
Cuts at: 0 665 1466 1532 1955 2169
Size: 665 801 66 423 214
Fragments arranged by size:
801 665 423 214 66
BsmI GAATG_Cn'
Cuts at: 0 1154 1321 2169
Size: 1154 167 848
Fragments arranged by size:
1154 848 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 643 2169
Size: 643 1526
Bsp1286I G_dGCh'C
Cuts at: 0 181 665 704 1427 1466 1481 1532 1677
Size: 181 484 39 723 39 15 51 145
Cuts at: 1677 1955 2169
Size: 278 214
Fragments arranged by size:
723 484 278 214 181 145 51 39
39 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 121 635 766 2169
Size: 121 514 131 1403
Fragments arranged by size:
1403 514 131 121
BsrBI CCG'CTC
Cuts at: 0 102 141 578 692 2169
Size: 102 39 437 114 1477
Fragments arranged by size:
1477 437 114 102 39
BsrDI GCAATG_nn'
Cuts at: 0 915 2071 2169
Size: 915 1156 98
Fragments arranged by size:
1156 915 98
BsrFI r'CCGG_y
Cuts at: 0 449 2169
Size: 449 1720
BsrGI T'GTAC_A
Cuts at: 0 34 1749 2169
Size: 34 1715 420
Fragments arranged by size:
1715 420 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 588 758 782 935 2169
Size: 588 170 24 153 1234
Fragments arranged by size:
1234 588 170 153 24
BstEII G'GTnAC_C
Cuts at: 0 534 2169
Size: 534 1635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1344 2169
Size: 1344 825
BstYI r'GATC_y
Cuts at: 0 863 1340 2169
Size: 863 477 829
Fragments arranged by size:
863 829 477
Bsu36I CC'TnA_GG
Cuts at: 0 322 1497 2169
Size: 322 1175 672
Fragments arranged by size:
1175 672 322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 1005 2169
Size: 720 285 1164
Fragments arranged by size:
1164 720 285
BtsI GCAGTG_nn'
Cuts at: 0 159 582 2169
Size: 159 423 1587
Fragments arranged by size:
1587 423 159
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 415 450 2169
Size: 415 35 1719
Fragments arranged by size:
1719 415 35
DraI TTT'AAA
Cuts at: 0 1095 2031 2169
Size: 1095 936 138
Fragments arranged by size:
1095 936 138
DraIII CAC_nnn'GTG
Cuts at: 0 420 2169
Size: 420 1749
EaeI y'GGCC_r
Cuts at: 0 809 957 1311 2169
Size: 809 148 354 858
Fragments arranged by size:
858 809 354 148
EarI CTCTTCn'nnn_
Cuts at: 0 1015 1471 2169
Size: 1015 456 698
Fragments arranged by size:
1015 698 456
EcoICRI GAG'CTC
Cuts at: 0 1464 1953 2169
Size: 1464 489 216
Fragments arranged by size:
1464 489 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1144 1321 1608 1678 1743 1751 2011
Size: 996 148 177 287 70 65 8 260
Cuts at: 2011 2140 2155 2169
Size: 129 15 14
Fragments arranged by size:
996 287 260 177 148 129 70 65
15 14 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 712 2169
Size: 712 1457
EcoO109I rG'GnC_Cy
Cuts at: 0 1477 2127 2169
Size: 1477 650 42
Fragments arranged by size:
1477 650 42
EcoRI G'AATT_C
Cuts at: 0 1069 1906 2169
Size: 1069 837 263
Fragments arranged by size:
1069 837 263
EcoRV GAT'ATC
Cuts at: 0 2076 2169
Size: 2076 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 699 2169
Size: 105 594 1470
Fragments arranged by size:
1470 594 105
FspI TGC'GCA
Cuts at: 0 358 1150 2169
Size: 358 792 1019
Fragments arranged by size:
1019 792 358
HaeII r_GCGC'y
Cuts at: 0 651 684 750 845 1019 2169
Size: 651 33 66 95 174 1150
Fragments arranged by size:
1150 651 174 95 66 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 679 711 973 1005 1182 1214
Size: 36 32 611 32 262 32 177 32
Cuts at: 1214 1257 1289 1729 1761 1876 1908 2169
Size: 43 32 440 32 115 32 261
Fragments arranged by size:
611 440 262 261 177 115 43 36
32 32 32 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 432 1817 2169
Size: 432 1385 352
Fragments arranged by size:
1385 432 352
MscI TGG'CCA
Cuts at: 0 811 959 1313 2169
Size: 811 148 354 856
Fragments arranged by size:
856 811 354 148
MslI CAynn'nnrTG
Cuts at: 0 803 1255 2169
Size: 803 452 914
Fragments arranged by size:
914 803 452
MspA1I CmG'CkG
Cuts at: 0 281 516 612 2169
Size: 281 235 96 1557
Fragments arranged by size:
1557 281 235 96
NaeI GCC'GGC
Cuts at: 0 451 2169
Size: 451 1718
NgoMIV G'CCGG_C
Cuts at: 0 449 2169
Size: 449 1720
NspI r_CATG'y
Cuts at: 0 591 657 1874 2169
Size: 591 66 1217 295
Fragments arranged by size:
1217 591 295 66
PciI A'CATG_T
Cuts at: 0 1870 2169
Size: 1870 299
PflMI CCAn_nnn'nTGG
Cuts at: 0 1836 2169
Size: 1836 333
PspOMI G'GGCC_C
Cuts at: 0 700 1477 2169
Size: 700 777 692
Fragments arranged by size:
777 700 692
PstI C_TGCA'G
Cuts at: 0 521 1331 2169
Size: 521 810 838
Fragments arranged by size:
838 810 521
PvuII CAG'CTG
Cuts at: 0 516 612 2169
Size: 516 96 1557
Fragments arranged by size:
1557 516 96
SacI G_AGCT'C
Cuts at: 0 1466 1955 2169
Size: 1466 489 214
Fragments arranged by size:
1466 489 214
SapI GCTCTTCn'nnn_
Cuts at: 0 1471 2169
Size: 1471 698
ScaI AGT'ACT
Cuts at: 0 901 2169
Size: 901 1268
SexAI A'CCwGG_T
Cuts at: 0 416 2169
Size: 416 1753
SfcI C'TryA_G
Cuts at: 0 517 594 1327 1380 2169
Size: 517 77 733 53 789
Fragments arranged by size:
789 733 517 77 53
SmlI C'TyrA_G
Cuts at: 0 673 787 1141 2169
Size: 673 114 354 1028
Fragments arranged by size:
1028 673 354 114
SphI G_CATG'C
Cuts at: 0 591 2169
Size: 591 1578
StuI AGG'CCT
Cuts at: 0 793 1386 1663 2169
Size: 793 593 277 506
Fragments arranged by size:
793 593 506 277
StyI C'CwwG_G
Cuts at: 0 1289 1427 1646 1856 2169
Size: 1289 138 219 210 313
Fragments arranged by size:
1289 313 219 210 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 893 1585 2169
Size: 893 692 584
Fragments arranged by size:
893 692 584
TatI w'GTAC_w
Cuts at: 0 34 899 1749 2169
Size: 34 865 850 420
Fragments arranged by size:
865 850 420 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 897 2169
Size: 865 32 1272
Fragments arranged by size:
1272 865 32
Tth111I GACn'n_nGTC
Cuts at: 0 823 2169
Size: 823 1346
XbaI T'CTAG_A
Cuts at: 0 1200 2169
Size: 1200 969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 35 414 1344 1653 2169
Size: 35 379 930 309 516
Fragments arranged by size:
930 516 379 309 35
XmnI GAAnn'nnTTC
Cuts at: 0 547 2164 2169
Size: 547 1617 5
Fragments arranged by size:
1617 547 5
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanII BbsI BglII Bme1580I
BmgBI BmrI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgZI BtsI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I MfeI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AloI
AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmtI BplI
Bpu10I BsaI BsaWI BseYI BsiWI BspEI BspHI BssHII
BssSI BstBI BstZ17I BtgI ClaI DrdI EagI EciI
FseI FspAI HincII HindIII HpaI KasI KpnI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XhoI XmaI ZraI