Restriction map for
pFN21AE4204
(Linear) (Six-base) MAPSORT of: KIEE4204.seq Check: 8860 from: 1 to: 2133
KIEE4204.orf
With 182 enzymes: SgfI *
March 22, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 506 705 1647 2133
Size: 506 199 942 486
Fragments arranged by size:
942 506 486 199
AccI GT'mk_AC
Cuts at: 0 596 1388 2133
Size: 596 792 745
Fragments arranged by size:
792 745 596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 892 1499 1743 2133
Size: 131 761 607 244 390
Fragments arranged by size:
761 607 390 244 131
AgeI A'CCGG_T
Cuts at: 0 2006 2133
Size: 2006 127
AleI CACnn'nnGTG
Cuts at: 0 1015 2133
Size: 1015 1118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 2133
Size: 961 1172
AlwNI CAG_nnn'CTG
Cuts at: 0 745 1291 2133
Size: 745 546 842
Fragments arranged by size:
842 745 546
ApaLI G'TGCA_C
Cuts at: 0 565 2133
Size: 565 1568
BamHI G'GATC_C
Cuts at: 0 188 1887 2133
Size: 188 1699 246
Fragments arranged by size:
1699 246 188
BanI G'GyrC_C
Cuts at: 0 1002 1946 2133
Size: 1002 944 187
Fragments arranged by size:
1002 944 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 1430 1505 2133
Size: 1430 75 628
Fragments arranged by size:
1430 628 75
BbvCI CC'TCA_GC
Cuts at: 0 1712 2133
Size: 1712 421
BclI T'GATC_A
Cuts at: 0 1341 2133
Size: 1341 792
BglI GCCn_nnn'nGGC
Cuts at: 0 1980 2133
Size: 1980 153
BlpI GC'TnA_GC
Cuts at: 0 1583 2133
Size: 1583 550
Bme1580I G_kGCm'C
Cuts at: 0 569 2133
Size: 569 1564
BmrI ACTGGGnnnn_n'
Cuts at: 0 1423 1479 2067 2133
Size: 1423 56 588 66
Fragments arranged by size:
1423 588 66 56
BmtI G_CTAG'C
Cuts at: 0 122 667 671 2133
Size: 122 545 4 1462
Fragments arranged by size:
1462 545 122 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 1696 2133
Size: 10 1686 437
Fragments arranged by size:
1686 437 10
Bpu10I CC'TnA_GC
Cuts at: 0 530 1712 2133
Size: 530 1182 421
Fragments arranged by size:
1182 530 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 891 1654 2133
Size: 820 71 763 479
Fragments arranged by size:
820 763 479 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 1672 2015 2133
Size: 1672 343 118
Fragments arranged by size:
1672 343 118
BsaHI Gr'CG_yC
Cuts at: 0 992 2133
Size: 992 1141
BsaWI w'CCGG_w
Cuts at: 0 2006 2133
Size: 2006 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 1669 1699 2133
Size: 850 30 789 30 434
Fragments arranged by size:
850 789 434 30 30
BseYI C'CCAG_C
Cuts at: 0 601 1543 1859 2037 2133
Size: 601 942 316 178 96
Fragments arranged by size:
942 601 316 178 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 669 1178 2133
Size: 231 438 509 955
Fragments arranged by size:
955 509 438 231
BsiHKAI G_wGCw'C
Cuts at: 0 41 557 569 660 2133
Size: 41 516 12 91 1473
Fragments arranged by size:
1473 516 91 41 12
BsmI GAATG_Cn'
Cuts at: 0 273 361 2133
Size: 273 88 1772
Fragments arranged by size:
1772 273 88
Bsp1286I G_dGCh'C
Cuts at: 0 41 557 569 660 2133
Size: 41 516 12 91 1473
Fragments arranged by size:
1473 516 91 41 12
BspHI T'CATG_A
Cuts at: 0 2046 2133
Size: 2046 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 705 1647 1810 2133
Size: 506 199 942 163 323
Fragments arranged by size:
942 506 323 199 163
BsrDI GCAATG_nn'
Cuts at: 0 1123 1969 2133
Size: 1123 846 164
Fragments arranged by size:
1123 846 164
BsrFI r'CCGG_y
Cuts at: 0 2006 2133
Size: 2006 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 277 367 1901 2133
Size: 277 90 1534 232
Fragments arranged by size:
1534 277 232 90
BstEII G'GTnAC_C
Cuts at: 0 1238 2133
Size: 1238 895
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1991 2133
Size: 1991 142
BstYI r'GATC_y
Cuts at: 0 188 397 465 1869 1887 2133
Size: 188 209 68 1404 18 246
Fragments arranged by size:
1404 246 209 188 68 18
Bsu36I CC'TnA_GG
Cuts at: 0 1079 2133
Size: 1079 1054
BtsI GCAGTG_nn'
Cuts at: 0 746 2133
Size: 746 1387
DraI TTT'AAA
Cuts at: 0 1412 1689 2133
Size: 1412 277 444
Fragments arranged by size:
1412 444 277
DraIII CAC_nnn'GTG
Cuts at: 0 573 2133
Size: 573 1560
EaeI y'GGCC_r
Cuts at: 0 717 2133
Size: 717 1416
EarI CTCTTCn'nnn_
Cuts at: 0 220 1611 2117 2133
Size: 220 1391 506 16
Fragments arranged by size:
1391 506 220 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 131 892 1499 1696 1743 2133
Size: 10 121 761 607 197 47 390
Fragments arranged by size:
761 607 390 197 121 47 10
EcoRV GAT'ATC
Cuts at: 0 318 2133
Size: 318 1815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 328 2133
Size: 328 1805
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 180 212 1303 1335 1543 1575 2133
Size: 180 32 1091 32 208 32 558
Fragments arranged by size:
1091 558 208 180 32 32 32
HincII GTy'rAC
Cuts at: 0 510 2133
Size: 510 1623
HpaI GTT'AAC
Cuts at: 0 510 2133
Size: 510 1623
MfeI C'AATT_G
Cuts at: 0 701 2133
Size: 701 1432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1627 2133
Size: 836 791 506
Fragments arranged by size:
836 791 506
MscI TGG'CCA
Cuts at: 0 719 2133
Size: 719 1414
MslI CAynn'nnrTG
Cuts at: 0 1015 2133
Size: 1015 1118
NheI G'CTAG_C
Cuts at: 0 118 663 667 2133
Size: 118 545 4 1466
Fragments arranged by size:
1466 545 118 4
NspI r_CATG'y
Cuts at: 0 113 545 2133
Size: 113 432 1588
Fragments arranged by size:
1588 432 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 1746 2133
Size: 1746 387
PfoI T'CCnGG_A
Cuts at: 0 1673 2133
Size: 1673 460
PstI C_TGCA'G
Cuts at: 0 741 2133
Size: 741 1392
ScaI AGT'ACT
Cuts at: 0 1831 2133
Size: 1831 302
SexAI A'CCwGG_T
Cuts at: 0 1458 1681 2133
Size: 1458 223 452
Fragments arranged by size:
1458 452 223
SfcI C'TryA_G
Cuts at: 0 233 737 1878 2133
Size: 233 504 1141 255
Fragments arranged by size:
1141 504 255 233
SmlI C'TyrA_G
Cuts at: 0 799 906 1633 2133
Size: 799 107 727 500
Fragments arranged by size:
799 727 500 107
StuI AGG'CCT
Cuts at: 0 609 1093 2133
Size: 609 484 1040
Fragments arranged by size:
1040 609 484
StyI C'CwwG_G
Cuts at: 0 347 479 1382 2133
Size: 347 132 903 751
Fragments arranged by size:
903 751 347 132
TatI w'GTAC_w
Cuts at: 0 1618 1718 1829 2133
Size: 1618 100 111 304
Fragments arranged by size:
1618 304 111 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 2133
Size: 850 32 1251
Fragments arranged by size:
1251 850 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 317 486 892 1782 2133
Size: 317 169 406 890 351
Fragments arranged by size:
890 406 351 317 169
XmnI GAAnn'nnTTC
Cuts at: 0 2020 2133
Size: 2020 113
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlfI AlwNI ApaLI
BamHI BanI BbsI BbvCI BclI BglI BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtsI
DraI DraIII EaeI EarI Eco57MI EcoRV FalI Hin4I
HincII HpaI MfeI MmeI MscI MslI NheI NspI
PflMI PfoI PstI ScaI SexAI SfcI SmlI StuI
StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AloI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BcgI BciVI BfrBI BglII BmgBI BplI
BsaAI BsaBI BseRI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI ClaI
CspCI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII HindIII KasI KpnI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmaI ZraI