Restriction map for
pFN21AE4202
(Linear) (Six-base) MAPSORT of: KIEE4202.seq Check: 8486 from: 1 to: 2124
KIEE4202.orf
With 182 enzymes: SgfI *
March 22, 2012 17:34 ..
AccI GT'mk_AC
Cuts at: 0 1511 1535 2124
Size: 1511 24 589
Fragments arranged by size:
1511 589 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 685 806 854 1568 2124
Size: 37 648 121 48 714 556
Fragments arranged by size:
714 648 556 121 48 37
AflIII A'CryG_T
Cuts at: 0 479 638 912 1223 1302 2124
Size: 479 159 274 311 79 822
Fragments arranged by size:
822 479 311 274 159 79
AgeI A'CCGG_T
Cuts at: 0 1502 1988 2124
Size: 1502 486 136
Fragments arranged by size:
1502 486 136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1827 2124
Size: 1827 297
AlwNI CAG_nnn'CTG
Cuts at: 0 60 2124
Size: 60 2064
ApaI G_GGCC'C
Cuts at: 0 196 330 446 2124
Size: 196 134 116 1678
Fragments arranged by size:
1678 196 134 116
ApaLI G'TGCA_C
Cuts at: 0 517 2124
Size: 517 1607
ApoI r'AATT_y
Cuts at: 0 568 580 690 718 1284 2124
Size: 568 12 110 28 566 840
Fragments arranged by size:
840 568 566 110 28 12
AvaI C'yCGr_G
Cuts at: 0 378 402 2124
Size: 378 24 1722
Fragments arranged by size:
1722 378 24
BamHI G'GATC_C
Cuts at: 0 1979 2124
Size: 1979 145
BanI G'GyrC_C
Cuts at: 0 263 1458 2124
Size: 263 1195 666
Fragments arranged by size:
1195 666 263
BanII G_rGCy'C
Cuts at: 0 196 330 433 446 2124
Size: 196 134 103 13 1678
Fragments arranged by size:
1678 196 134 103 13
BbeI G_GCGC'C
Cuts at: 0 267 2124
Size: 267 1857
BbsI GAAGACnn'nnnn_
Cuts at: 0 1505 1824 2124
Size: 1505 319 300
Fragments arranged by size:
1505 319 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1180 1214 2124
Size: 1180 34 910
Fragments arranged by size:
1180 910 34
BclI T'GATC_A
Cuts at: 0 1079 2124
Size: 1079 1045
BglI GCCn_nnn'nGGC
Cuts at: 0 97 2124
Size: 97 2027
BlpI GC'TnA_GC
Cuts at: 0 630 2124
Size: 630 1494
Bme1580I G_kGCm'C
Cuts at: 0 196 330 446 521 2124
Size: 196 134 116 75 1603
Fragments arranged by size:
1603 196 134 116 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 417 601 1004 2124
Size: 237 180 184 403 1120
Fragments arranged by size:
1120 403 237 184 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 329 550 1763 2124
Size: 82 247 221 1213 361
Fragments arranged by size:
1213 361 247 221 82
Bpu10I CC'TnA_GC
Cuts at: 0 649 874 1427 1789 2124
Size: 649 225 553 362 335
Fragments arranged by size:
649 553 362 335 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 2124
Size: 571 1553
BsaAI yAC'GTr
Cuts at: 0 1224 1974 2124
Size: 1224 750 150
Fragments arranged by size:
1224 750 150
BsaHI Gr'CG_yC
Cuts at: 0 264 2124
Size: 264 1860
BsaWI w'CCGG_w
Cuts at: 0 1502 1988 2124
Size: 1502 486 136
Fragments arranged by size:
1502 486 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1237 1267 2124
Size: 1237 30 857
Fragments arranged by size:
1237 857 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 2124
Size: 237 1887
BsiEI CG_ry'CG
Cuts at: 0 38 86 2124
Size: 38 48 2038
Fragments arranged by size:
2038 48 38
BsiHKAI G_wGCw'C
Cuts at: 0 433 521 2124
Size: 433 88 1603
Fragments arranged by size:
1603 433 88
BsmI GAATG_Cn'
Cuts at: 0 1329 2124
Size: 1329 795
BsmBI CGTCTCn'nnnn_
Cuts at: 0 73 2124
Size: 73 2051
Bsp1286I G_dGCh'C
Cuts at: 0 196 330 433 446 521 2124
Size: 196 134 103 13 75 1603
Fragments arranged by size:
1603 196 134 103 75 13
BsrBI CCG'CTC
Cuts at: 0 179 335 401 2124
Size: 179 156 66 1723
Fragments arranged by size:
1723 179 156 66
BsrDI GCAATG_nn'
Cuts at: 0 173 1514 2124
Size: 173 1341 610
Fragments arranged by size:
1341 610 173
BsrFI r'CCGG_y
Cuts at: 0 91 1502 1988 2124
Size: 91 1411 486 136
Fragments arranged by size:
1411 486 136 91
BsrGI T'GTAC_A
Cuts at: 0 482 641 2124
Size: 482 159 1483
Fragments arranged by size:
1483 482 159
BssSI C'ACGA_G
Cuts at: 0 520 2124
Size: 520 1604
BstAPI GCAn_nnn'nTGC
Cuts at: 0 525 2124
Size: 525 1599
BstYI r'GATC_y
Cuts at: 0 158 1760 1979 2124
Size: 158 1602 219 145
Fragments arranged by size:
1602 219 158 145
BstZ17I GTA'TAC
Cuts at: 0 1512 2124
Size: 1512 612
BtgI C'CryG_G
Cuts at: 0 38 2124
Size: 38 2086
BtsI GCAGTG_nn'
Cuts at: 0 20 225 980 1961 2124
Size: 20 205 755 981 163
Fragments arranged by size:
981 755 205 163 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 596 631 2124
Size: 596 35 1493
Fragments arranged by size:
1493 596 35
DraI TTT'AAA
Cuts at: 0 563 867 1404 2124
Size: 563 304 537 720
Fragments arranged by size:
720 563 537 304
DraIII CAC_nnn'GTG
Cuts at: 0 525 702 2124
Size: 525 177 1422
Fragments arranged by size:
1422 525 177
EaeI y'GGCC_r
Cuts at: 0 35 83 107 536 2124
Size: 35 48 24 429 1588
Fragments arranged by size:
1588 429 48 35 24
EagI C'GGCC_G
Cuts at: 0 35 83 2124
Size: 35 48 2041
Fragments arranged by size:
2041 48 35
EarI CTCTTCn'nnn_
Cuts at: 0 56 280 782 830 1715 1909 2124
Size: 56 224 502 48 885 194 215
Fragments arranged by size:
885 502 224 215 194 56 48
EcoICRI GAG'CTC
Cuts at: 0 431 2124
Size: 431 1693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 82 329 550 685 806 854 1568
Size: 37 45 247 221 135 121 48 714
Cuts at: 1568 1763 2124
Size: 195 361
Fragments arranged by size:
714 361 247 221 195 135 121 48
45 37
EcoO109I rG'GnC_Cy
Cuts at: 0 270 442 2124
Size: 270 172 1682
Fragments arranged by size:
1682 270 172
FspI TGC'GCA
Cuts at: 0 421 2124
Size: 421 1703
HaeII r_GCGC'y
Cuts at: 0 267 2124
Size: 267 1857
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1597 1629 2061 2093 2124
Size: 1597 32 432 32 31
Fragments arranged by size:
1597 432 32 32 31
HindIII A'AGCT_T
Cuts at: 0 1770 2124
Size: 1770 354
KasI G'GCGC_C
Cuts at: 0 263 2124
Size: 263 1861
MslI CAynn'nnrTG
Cuts at: 0 1075 1190 2124
Size: 1075 115 934
Fragments arranged by size:
1075 934 115
MspA1I CmG'CkG
Cuts at: 0 40 128 261 2124
Size: 40 88 133 1863
Fragments arranged by size:
1863 133 88 40
NaeI GCC'GGC
Cuts at: 0 93 2124
Size: 93 2031
NarI GG'CG_CC
Cuts at: 0 264 2124
Size: 264 1860
NgoMIV G'CCGG_C
Cuts at: 0 91 2124
Size: 91 2033
NotI GC'GGCC_GC
Cuts at: 0 35 2124
Size: 35 2089
NspI r_CATG'y
Cuts at: 0 483 642 916 988 1022 1201 1306 2124
Size: 483 159 274 72 34 179 105 818
Fragments arranged by size:
818 483 274 179 159 105 72 34
PciI A'CATG_T
Cuts at: 0 479 638 912 1302 2124
Size: 479 159 274 390 822
Fragments arranged by size:
822 479 390 274 159
PmlI CAC'GTG
Cuts at: 0 1974 2124
Size: 1974 150
PsiI TTA'TAA
Cuts at: 0 1421 2124
Size: 1421 703
PspOMI G'GGCC_C
Cuts at: 0 192 326 442 2124
Size: 192 134 116 1682
Fragments arranged by size:
1682 192 134 116
SacI G_AGCT'C
Cuts at: 0 433 2124
Size: 433 1691
SacII CC_GC'GG
Cuts at: 0 41 2124
Size: 41 2083
ScaI AGT'ACT
Cuts at: 0 361 2124
Size: 361 1763
SfcI C'TryA_G
Cuts at: 0 1265 1365 1532 2124
Size: 1265 100 167 592
Fragments arranged by size:
1265 592 167 100
SfoI GGC'GCC
Cuts at: 0 265 2124
Size: 265 1859
SmlI C'TyrA_G
Cuts at: 0 586 2124
Size: 586 1538
TatI w'GTAC_w
Cuts at: 0 359 476 482 641 2124
Size: 359 117 6 159 1483
Fragments arranged by size:
1483 359 159 117 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1842 1874 2124
Size: 199 32 1611 32 250
Fragments arranged by size:
1611 250 199 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 791 1329 2124
Size: 791 538 795
Fragments arranged by size:
795 791 538
Enzymes that do cut:
AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BclI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
HindIII KasI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PmlI PsiI PspOMI SacI SacII ScaI
SfcI SfoI SmlI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BfrBI BglII BmgBI BmrI BmtI BsaI BsaBI BseRI
BseYI BsiWI BspEI BspHI BspMI BssHII BstBI BstEII
BstXI Bsu36I BtgZI ClaI DrdI EciI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NruI NsiI
PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI