Restriction map for
pFN21AE4201
(Linear) (Six-base) MAPSORT of: KIEE4201.seq Check: 237 from: 1 to: 2121
KIEE4201.orf
With 182 enzymes: SgfI *
March 22, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1759 2121
Size: 1759 362
AccI GT'mk_AC
Cuts at: 0 1145 2121
Size: 1145 976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1820 2121
Size: 1820 301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 2121
Size: 505 1616
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 862 894 2121
Size: 862 32 1227
Fragments arranged by size:
1227 862 32
AlwNI CAG_nnn'CTG
Cuts at: 0 469 2007 2121
Size: 469 1538 114
Fragments arranged by size:
1538 469 114
ApaLI G'TGCA_C
Cuts at: 0 1282 2121
Size: 1282 839
AvaI C'yCGr_G
Cuts at: 0 49 734 1251 2121
Size: 49 685 517 870
Fragments arranged by size:
870 685 517 49
BamHI G'GATC_C
Cuts at: 0 116 252 2121
Size: 116 136 1869
Fragments arranged by size:
1869 136 116
BanI G'GyrC_C
Cuts at: 0 861 1198 1539 1967 2121
Size: 861 337 341 428 154
Fragments arranged by size:
861 428 341 337 154
BanII G_rGCy'C
Cuts at: 0 308 813 1103 1252 1657 1942 2121
Size: 308 505 290 149 405 285 179
Fragments arranged by size:
505 405 308 290 285 179 149
BbeI G_GCGC'C
Cuts at: 0 1971 2121
Size: 1971 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 133 204 2121
Size: 133 71 1917
Fragments arranged by size:
1917 133 71
BbvCI CC'TCA_GC
Cuts at: 0 443 1259 1958 2121
Size: 443 816 699 163
Fragments arranged by size:
816 699 443 163
BglI GCCn_nnn'nGGC
Cuts at: 0 1828 2121
Size: 1828 293
Bme1580I G_kGCm'C
Cuts at: 0 1286 1544 2121
Size: 1286 258 577
Fragments arranged by size:
1286 577 258
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 288 400 627 1538 1589 2121
Size: 29 259 112 227 911 51 532
Fragments arranged by size:
911 532 259 227 112 51 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1465 1663 1890 2046 2121
Size: 120 1345 198 227 156 75
Fragments arranged by size:
1345 227 198 156 120 75
Bpu10I CC'TnA_GC
Cuts at: 0 443 1206 1259 1958 2121
Size: 443 763 53 699 163
Fragments arranged by size:
763 699 443 163 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 2074 2121
Size: 169 1905 47
Fragments arranged by size:
1905 169 47
BsaAI yAC'GTr
Cuts at: 0 1044 1356 2121
Size: 1044 312 765
Fragments arranged by size:
1044 765 312
BsaBI GATnn'nnATC
Cuts at: 0 1186 2121
Size: 1186 935
BsaHI Gr'CG_yC
Cuts at: 0 1968 2121
Size: 1968 153
BsaWI w'CCGG_w
Cuts at: 0 178 2121
Size: 178 1943
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 1015 1045 1888 1918 2121
Size: 862 30 123 30 843 30 203
Fragments arranged by size:
862 843 203 123 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 302 355 586 1000 2121
Size: 271 31 53 231 414 1121
Fragments arranged by size:
1121 414 271 231 53 31
BseYI C'CCAG_C
Cuts at: 0 519 1113 1400 1861 2121
Size: 519 594 287 461 260
Fragments arranged by size:
594 519 461 287 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1072 1249 2121
Size: 991 81 177 872
Fragments arranged by size:
991 872 177 81
BsiHKAI G_wGCw'C
Cuts at: 0 1103 1286 1597 2121
Size: 1103 183 311 524
Fragments arranged by size:
1103 524 311 183
BsmI GAATG_Cn'
Cuts at: 0 533 942 2121
Size: 533 409 1179
Fragments arranged by size:
1179 533 409
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1573 2121
Size: 1573 548
Bsp1286I G_dGCh'C
Cuts at: 0 308 813 1103 1252 1286 1544 1597 1657
Size: 308 505 290 149 34 258 53 60
Cuts at: 1657 1942 2121
Size: 285 179
Fragments arranged by size:
505 308 290 285 258 179 149 60
53 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 948 1697 1759 2121
Size: 395 553 749 62 362
Fragments arranged by size:
749 553 395 362 62
BsrBI CCG'CTC
Cuts at: 0 149 1506 2121
Size: 149 1357 615
Fragments arranged by size:
1357 615 149
BsrDI GCAATG_nn'
Cuts at: 0 513 899 1488 2121
Size: 513 386 589 633
Fragments arranged by size:
633 589 513 386
BsrFI r'CCGG_y
Cuts at: 0 422 787 1827 2121
Size: 422 365 1040 294
Fragments arranged by size:
1040 422 365 294
BstEII G'GTnAC_C
Cuts at: 0 382 1128 2121
Size: 382 746 993
Fragments arranged by size:
993 746 382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 301 1743 2054 2121
Size: 249 52 1442 311 67
Fragments arranged by size:
1442 311 249 67 52
BstYI r'GATC_y
Cuts at: 0 116 252 797 1433 2121
Size: 116 136 545 636 688
Fragments arranged by size:
688 636 545 136 116
Bsu36I CC'TnA_GG
Cuts at: 0 558 569 2121
Size: 558 11 1552
Fragments arranged by size:
1552 558 11
BtgI C'CryG_G
Cuts at: 0 267 1383 1607 1742 1932 1971 2053 2121
Size: 267 1116 224 135 190 39 82 68
Fragments arranged by size:
1116 267 224 190 135 82 68 39
BtsI GCAGTG_nn'
Cuts at: 0 1479 2121
Size: 1479 642
ClaI AT'CG_AT
Cuts at: 0 351 2121
Size: 351 1770
DrdI GACnn_nn'nnGTC
Cuts at: 0 208 1090 2121
Size: 208 882 1031
Fragments arranged by size:
1031 882 208
EaeI y'GGCC_r
Cuts at: 0 460 1698 1718 1755 1926 2035 2121
Size: 460 1238 20 37 171 109 86
Fragments arranged by size:
1238 460 171 109 86 37 20
EarI CTCTTCn'nnn_
Cuts at: 0 379 1270 1564 2121
Size: 379 891 294 557
Fragments arranged by size:
891 557 379 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 432 835 2121
Size: 432 403 1286
Fragments arranged by size:
1286 432 403
EcoICRI GAG'CTC
Cuts at: 0 1101 2121
Size: 1101 1020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1465 1663 1820 1890 2046 2121
Size: 120 1345 198 157 70 156 75
Fragments arranged by size:
1345 198 157 156 120 75 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1756 2121
Size: 1756 365
EcoO109I rG'GnC_Cy
Cuts at: 0 30 43 201 439 1110 1255 1728 1761
Size: 30 13 158 238 671 145 473 33
Cuts at: 1761 2121
Size: 360
Fragments arranged by size:
671 473 360 238 158 145 33 30
13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 2121
Size: 1080 1041
FspI TGC'GCA
Cuts at: 0 238 1069 2121
Size: 238 831 1052
Fragments arranged by size:
1052 831 238
HaeII r_GCGC'y
Cuts at: 0 727 1686 1971 2121
Size: 727 959 285 150
Fragments arranged by size:
959 727 285 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 710 742 1425 1457 2121
Size: 710 32 683 32 664
Fragments arranged by size:
710 683 664 32 32
KasI G'GCGC_C
Cuts at: 0 1967 2121
Size: 1967 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 326 498 1295 2055 2121
Size: 230 96 172 797 760 66
Fragments arranged by size:
797 760 230 172 96 66
MscI TGG'CCA
Cuts at: 0 1700 1720 1928 2121
Size: 1700 20 208 193
Fragments arranged by size:
1700 208 193 20
MslI CAynn'nnrTG
Cuts at: 0 68 928 1741 2121
Size: 68 860 813 380
Fragments arranged by size:
860 813 380 68
MspA1I CmG'CkG
Cuts at: 0 10 678 1117 1385 1587 1803 2066 2121
Size: 10 668 439 268 202 216 263 55
Fragments arranged by size:
668 439 268 263 216 202 55 10
NaeI GCC'GGC
Cuts at: 0 1829 2121
Size: 1829 292
NarI GG'CG_CC
Cuts at: 0 1968 2121
Size: 1968 153
NcoI C'CATG_G
Cuts at: 0 1742 2121
Size: 1742 379
NgoMIV G'CCGG_C
Cuts at: 0 1827 2121
Size: 1827 294
PasI CC'CwG_GG
Cuts at: 0 257 2121
Size: 257 1864
PflMI CCAn_nnn'nTGG
Cuts at: 0 953 1536 2007 2121
Size: 953 583 471 114
Fragments arranged by size:
953 583 471 114
PfoI T'CCnGG_A
Cuts at: 0 962 2121
Size: 962 1159
PmlI CAC'GTG
Cuts at: 0 1356 2121
Size: 1356 765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 2121
Size: 862 32 1227
Fragments arranged by size:
1227 862 32
PpuMI rG'GwC_Cy
Cuts at: 0 30 201 1110 1728 2121
Size: 30 171 909 618 393
Fragments arranged by size:
909 618 393 171 30
PstI C_TGCA'G
Cuts at: 0 21 332 818 1474 1770 2121
Size: 21 311 486 656 296 351
Fragments arranged by size:
656 486 351 311 296 21
PvuII CAG'CTG
Cuts at: 0 678 1803 2121
Size: 678 1125 318
Fragments arranged by size:
1125 678 318
SacI G_AGCT'C
Cuts at: 0 1103 2121
Size: 1103 1018
SacII CC_GC'GG
Cuts at: 0 1386 2121
Size: 1386 735
SanDI GG'GwC_CC
Cuts at: 0 30 201 1110 2121
Size: 30 171 909 1011
Fragments arranged by size:
1011 909 171 30
SapI GCTCTTCn'nnn_
Cuts at: 0 1270 1564 2121
Size: 1270 294 557
Fragments arranged by size:
1270 557 294
SbfI CC_TGCA'GG
Cuts at: 0 1770 2121
Size: 1770 351
SfcI C'TryA_G
Cuts at: 0 17 206 328 648 814 1470 1766 2121
Size: 17 189 122 320 166 656 296 355
Fragments arranged by size:
656 355 320 296 189 166 122 17
SfoI GGC'GCC
Cuts at: 0 1969 2121
Size: 1969 152
SmaI CCC'GGG
Cuts at: 0 51 736 2121
Size: 51 685 1385
Fragments arranged by size:
1385 685 51
StuI AGG'CCT
Cuts at: 0 874 2121
Size: 874 1247
StyI C'CwwG_G
Cuts at: 0 501 1742 2121
Size: 501 1241 379
Fragments arranged by size:
1241 501 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 745 1414 1635 2055 2121
Size: 745 669 221 420 66
Fragments arranged by size:
745 669 420 221 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 386 418 1888 1920 2121
Size: 386 32 1470 32 201
Fragments arranged by size:
1470 386 201 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1365 2121
Size: 1365 756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 696 717 1041 1713 2121
Size: 696 21 324 672 408
Fragments arranged by size:
696 672 408 324 21
XmaI C'CCGG_G
Cuts at: 0 49 734 2121
Size: 49 685 1387
Fragments arranged by size:
1387 685 49
XmnI GAAnn'nnTTC
Cuts at: 0 59 2121
Size: 59 2062
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PmlI PpiI PpuMI PstI PvuII
SacI SacII SanDI SapI SbfI SfcI SfoI SmaI
StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsiEI BsiWI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI CspCI DraI DraIII
EagI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XhoI ZraI