Restriction map for
pFN21AE4182
(Linear) (Six-base) MAPSORT of: KIEE4182.seq Check: 8785 from: 1 to: 2181
KIEE4182.orf
With 182 enzymes: SgfI *
March 22, 2012 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 849 2181
Size: 849 1332
AccI GT'mk_AC
Cuts at: 0 198 431 1795 1873 2181
Size: 198 233 1364 78 308
Fragments arranged by size:
1364 308 233 198 78
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 975 1136 1352 1756 1954 2181
Size: 700 275 161 216 404 198 227
Fragments arranged by size:
700 404 275 227 216 198 161
AflII C'TTAA_G
Cuts at: 0 949 2181
Size: 949 1232
AflIII A'CryG_T
Cuts at: 0 112 1753 2181
Size: 112 1641 428
Fragments arranged by size:
1641 428 112
AlwNI CAG_nnn'CTG
Cuts at: 0 1527 2181
Size: 1527 654
ApoI r'AATT_y
Cuts at: 0 167 453 648 884 943 2181
Size: 167 286 195 236 59 1238
Fragments arranged by size:
1238 286 236 195 167 59
AvaI C'yCGr_G
Cuts at: 0 1637 2181
Size: 1637 544
AvrII C'CTAG_G
Cuts at: 0 1090 2181
Size: 1090 1091
BanII G_rGCy'C
Cuts at: 0 89 1198 2181
Size: 89 1109 983
Fragments arranged by size:
1109 983 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 2181
Size: 403 1778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1424 1458 2181
Size: 1424 34 723
Fragments arranged by size:
1424 723 34
BfrBI ATG'CAT
Cuts at: 0 856 2181
Size: 856 1325
BglI GCCn_nnn'nGGC
Cuts at: 0 1717 2181
Size: 1717 464
BglII A'GATC_T
Cuts at: 0 1609 2181
Size: 1609 572
BmrI ACTGGGnnnn_n'
Cuts at: 0 1796 1839 2181
Size: 1796 43 342
Fragments arranged by size:
1796 342 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 2181
Size: 417 1764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 1291 1295 1789 2181
Size: 858 433 4 494 392
Fragments arranged by size:
858 494 433 392 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1309 2181
Size: 1309 872
BsaAI yAC'GTr
Cuts at: 0 1756 2181
Size: 1756 425
BsaBI GATnn'nnATC
Cuts at: 0 924 2181
Size: 924 1257
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 749 1004 2139 2181
Size: 749 255 1135 42
Fragments arranged by size:
1135 749 255 42
BseYI C'CCAG_C
Cuts at: 0 965 2181
Size: 965 1216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1418 2138 2181
Size: 767 651 720 43
Fragments arranged by size:
767 720 651 43
BsiHKAI G_wGCw'C
Cuts at: 0 89 1729 1753 2181
Size: 89 1640 24 428
Fragments arranged by size:
1640 428 89 24
BsmI GAATG_Cn'
Cuts at: 0 858 2047 2181
Size: 858 1189 134
Fragments arranged by size:
1189 858 134
Bsp1286I G_dGCh'C
Cuts at: 0 89 1198 1729 1753 2181
Size: 89 1109 531 24 428
Fragments arranged by size:
1109 531 428 89 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 849 2181
Size: 849 1332
BssSI C'ACGA_G
Cuts at: 0 2137 2181
Size: 2137 44
BstBI TT'CG_AA
Cuts at: 0 171 2181
Size: 171 2010
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1885 2181
Size: 1885 296
BstYI r'GATC_y
Cuts at: 0 1609 2181
Size: 1609 572
BstZ17I GTA'TAC
Cuts at: 0 432 2181
Size: 432 1749
BtgI C'CryG_G
Cuts at: 0 80 1878 2181
Size: 80 1798 303
Fragments arranged by size:
1798 303 80
BtsI GCAGTG_nn'
Cuts at: 0 779 2181
Size: 779 1402
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2132 2167 2181
Size: 2132 35 14
Fragments arranged by size:
2132 35 14
EaeI y'GGCC_r
Cuts at: 0 1718 2181
Size: 1718 463
EarI CTCTTCn'nnn_
Cuts at: 0 94 318 513 1427 1973 2181
Size: 94 224 195 914 546 208
Fragments arranged by size:
914 546 224 208 195 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 320 851 2181
Size: 320 531 1330
Fragments arranged by size:
1330 531 320
EcoICRI GAG'CTC
Cuts at: 0 87 2181
Size: 87 2094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 858 975 1136 1291 1295 1352 1756
Size: 700 158 117 161 155 4 57 404
Cuts at: 1756 1789 1954 2181
Size: 33 165 227
Fragments arranged by size:
700 404 227 165 161 158 155 117
57 33 4
EcoO109I rG'GnC_Cy
Cuts at: 0 810 2181
Size: 810 1371
EcoRV GAT'ATC
Cuts at: 0 1644 2181
Size: 1644 537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1984 2181
Size: 1984 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 726 758 1462 1494 2181
Size: 371 32 323 32 704 32 687
Fragments arranged by size:
704 687 371 323 32 32 32
HincII GTy'rAC
Cuts at: 0 199 2181
Size: 199 1982
HindIII A'AGCT_T
Cuts at: 0 2167 2181
Size: 2167 14
MfeI C'AATT_G
Cuts at: 0 448 2181
Size: 448 1733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1866 2181
Size: 1866 315
MslI CAynn'nnrTG
Cuts at: 0 71 533 1651 2078 2181
Size: 71 462 1118 427 103
Fragments arranged by size:
1118 462 427 103 71
MspA1I CmG'CkG
Cuts at: 0 1241 2181
Size: 1241 940
NcoI C'CATG_G
Cuts at: 0 80 1878 2181
Size: 80 1798 303
Fragments arranged by size:
1798 303 80
NsiI A_TGCA'T
Cuts at: 0 858 2181
Size: 858 1323
NspI r_CATG'y
Cuts at: 0 116 538 1333 2181
Size: 116 422 795 848
Fragments arranged by size:
848 795 422 116
PciI A'CATG_T
Cuts at: 0 112 2181
Size: 112 2069
PfoI T'CCnGG_A
Cuts at: 0 874 2181
Size: 874 1307
PmlI CAC'GTG
Cuts at: 0 1756 2181
Size: 1756 425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 2181
Size: 558 32 1591
Fragments arranged by size:
1591 558 32
PpuMI rG'GwC_Cy
Cuts at: 0 810 2181
Size: 810 1371
PshAI GACnn'nnGTC
Cuts at: 0 1413 2181
Size: 1413 768
PstI C_TGCA'G
Cuts at: 0 409 468 2181
Size: 409 59 1713
Fragments arranged by size:
1713 409 59
SacI G_AGCT'C
Cuts at: 0 89 2181
Size: 89 2092
SalI G'TCGA_C
Cuts at: 0 197 2181
Size: 197 1984
SapI GCTCTTCn'nnn_
Cuts at: 0 94 2181
Size: 94 2087
ScaI AGT'ACT
Cuts at: 0 364 2181
Size: 364 1817
SfcI C'TryA_G
Cuts at: 0 57 332 405 464 752 1504 2181
Size: 57 275 73 59 288 752 677
Fragments arranged by size:
752 677 288 275 73 59 57
SmlI C'TyrA_G
Cuts at: 0 949 1324 2181
Size: 949 375 857
Fragments arranged by size:
949 857 375
StyI C'CwwG_G
Cuts at: 0 80 1090 1878 2181
Size: 80 1010 788 303
Fragments arranged by size:
1010 788 303 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 761 2181
Size: 761 1420
TatI w'GTAC_w
Cuts at: 0 362 1933 2181
Size: 362 1571 248
Fragments arranged by size:
1571 362 248
XmnI GAAnn'nnTTC
Cuts at: 0 185 2181
Size: 185 1996
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlwNI ApoI AvaI
AvrII BanII BbsI BcgI BfrBI BglI BglII BmrI
BplI BpmI BpuEI BsaAI BsaBI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BssSI BstBI BstXI BstYI
BstZ17I BtgI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI
MmeI MslI MspA1I NcoI NsiI NspI PciI PfoI
PmlI PpiI PpuMI PshAI PstI SacI SalI SapI
ScaI SfcI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BbvCI BciVI BclI BlpI Bme1580I BmgBI
BmtI Bpu10I BsaI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstEII Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EcoNI EcoRI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI