Restriction map for
pFN21AE4172
(Linear) (Six-base) MAPSORT of: KIEE4172.seq Check: 7661 from: 1 to: 1899
KIEE4172.orf
With 182 enzymes: SgfI *
July 3, 2012 18:43 ..
AccI GT'mk_AC
Cuts at: 0 1032 1899
Size: 1032 867
AflIII A'CryG_T
Cuts at: 0 384 809 1899
Size: 384 425 1090
Fragments arranged by size:
1090 425 384
AlwNI CAG_nnn'CTG
Cuts at: 0 78 1899
Size: 78 1821
ApaI G_GGCC'C
Cuts at: 0 115 1899
Size: 115 1784
ApoI r'AATT_y
Cuts at: 0 256 628 1188 1316 1434 1606 1899
Size: 256 372 560 128 118 172 293
Fragments arranged by size:
560 372 293 256 172 128 118
BanI G'GyrC_C
Cuts at: 0 62 1899
Size: 62 1837
BanII G_rGCy'C
Cuts at: 0 115 1899
Size: 115 1784
BbsI GAAGACnn'nnnn_
Cuts at: 0 42 1675 1899
Size: 42 1633 224
Fragments arranged by size:
1633 224 42
BbvCI CC'TCA_GC
Cuts at: 0 130 1899
Size: 130 1769
BciVI GTATCCnnnnn_n'
Cuts at: 0 329 1899
Size: 329 1570
BfrBI ATG'CAT
Cuts at: 0 527 755 1595 1899
Size: 527 228 840 304
Fragments arranged by size:
840 527 304 228
BglI GCCn_nnn'nGGC
Cuts at: 0 119 1899
Size: 119 1780
BglII A'GATC_T
Cuts at: 0 533 1266 1899
Size: 533 733 633
Fragments arranged by size:
733 633 533
BlpI GC'TnA_GC
Cuts at: 0 55 1899
Size: 55 1844
Bme1580I G_kGCm'C
Cuts at: 0 115 1899
Size: 115 1784
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 1899
Size: 304 1595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1899
Size: 1001 898
Bpu10I CC'TnA_GC
Cuts at: 0 130 1899
Size: 130 1769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1899
Size: 63 1836
BsaBI GATnn'nnATC
Cuts at: 0 1730 1899
Size: 1730 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 965 995 1899
Size: 965 30 904
Fragments arranged by size:
965 904 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 154 1899
Size: 151 3 1745
Fragments arranged by size:
1745 151 3
BseYI C'CCAG_C
Cuts at: 0 239 1899
Size: 239 1660
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1899
Size: 609 1290
BsiHKAI G_wGCw'C
Cuts at: 0 1753 1899
Size: 1753 146
BsmI GAATG_Cn'
Cuts at: 0 757 1899
Size: 757 1142
Bsp1286I G_dGCh'C
Cuts at: 0 115 1753 1899
Size: 115 1638 146
Fragments arranged by size:
1638 146 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 1899
Size: 482 1417
BsrDI GCAATG_nn'
Cuts at: 0 1586 1899
Size: 1586 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1142 1899
Size: 1142 757
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1581 1899
Size: 1581 318
BstYI r'GATC_y
Cuts at: 0 533 1266 1899
Size: 533 733 633
Fragments arranged by size:
733 633 533
BtsI GCAGTG_nn'
Cuts at: 0 973 1202 1534 1574 1899
Size: 973 229 332 40 325
Fragments arranged by size:
973 332 325 229 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1899
Size: 1001 898
EcoNI CCTnn'n_nnAGG
Cuts at: 0 27 489 1899
Size: 27 462 1410
Fragments arranged by size:
1410 462 27
EcoO109I rG'GnC_Cy
Cuts at: 0 112 1899
Size: 112 1787
EcoRI G'AATT_C
Cuts at: 0 628 1899
Size: 628 1271
EcoRV GAT'ATC
Cuts at: 0 435 1899
Size: 435 1464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 475 1899
Size: 475 1424
HaeII r_GCGC'y
Cuts at: 0 79 1899
Size: 79 1820
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1899
Size: 424 32 1443
Fragments arranged by size:
1443 424 32
HincII GTy'rAC
Cuts at: 0 1063 1899
Size: 1063 836
HindIII A'AGCT_T
Cuts at: 0 262 472 1899
Size: 262 210 1427
Fragments arranged by size:
1427 262 210
MfeI C'AATT_G
Cuts at: 0 722 1743 1894 1899
Size: 722 1021 151 5
Fragments arranged by size:
1021 722 151 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1899
Size: 1008 891
MslI CAynn'nnrTG
Cuts at: 0 568 1579 1809 1899
Size: 568 1011 230 90
Fragments arranged by size:
1011 568 230 90
MspA1I CmG'CkG
Cuts at: 0 59 1899
Size: 59 1840
NsiI A_TGCA'T
Cuts at: 0 529 757 1597 1899
Size: 529 228 840 302
Fragments arranged by size:
840 529 302 228
NspI r_CATG'y
Cuts at: 0 388 1899
Size: 388 1511
PciI A'CATG_T
Cuts at: 0 384 1899
Size: 384 1515
PsiI TTA'TAA
Cuts at: 0 740 1460 1899
Size: 740 720 439
Fragments arranged by size:
740 720 439
PspOMI G'GGCC_C
Cuts at: 0 111 1899
Size: 111 1788
PstI C_TGCA'G
Cuts at: 0 660 1899
Size: 660 1239
PvuII CAG'CTG
Cuts at: 0 59 1899
Size: 59 1840
ScaI AGT'ACT
Cuts at: 0 1863 1899
Size: 1863 36
SfcI C'TryA_G
Cuts at: 0 656 1899
Size: 656 1243
SmlI C'TyrA_G
Cuts at: 0 42 1899
Size: 42 1857
SpeI A'CTAG_T
Cuts at: 0 648 1615 1899
Size: 648 967 284
Fragments arranged by size:
967 648 284
SspI AAT'ATT
Cuts at: 0 1563 1899
Size: 1563 336
StuI AGG'CCT
Cuts at: 0 22 1899
Size: 22 1877
StyI C'CwwG_G
Cuts at: 0 268 687 1899
Size: 268 419 1212
Fragments arranged by size:
1212 419 268
TatI w'GTAC_w
Cuts at: 0 553 1084 1861 1899
Size: 553 531 777 38
Fragments arranged by size:
777 553 531 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 965 997 1899
Size: 965 32 902
Fragments arranged by size:
965 902 32
XmnI GAAnn'nnTTC
Cuts at: 0 256 1889 1899
Size: 256 1633 10
Fragments arranged by size:
1633 256 10
Enzymes that do cut:
AccI AflIII AlwNI ApaI ApoI BanI BanII BbsI
BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI
BtsI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII MfeI MmeI MslI MspA1I NsiI
NspI PciI PsiI PspOMI PstI PvuII ScaI SfcI
SmlI SpeI SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BclI BmgBI
BmtI BplI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
FseI FspI FspAI HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI