Restriction map for
pFN21AE4170
(Linear) (Six-base) MAPSORT of: KIEE4170.seq Check: 2919 from: 1 to: 1863
KIEE4170.orf
With 182 enzymes: SgfI *
July 3, 2012 18:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1129 1342 1863
Size: 524 605 213 521
Fragments arranged by size:
605 524 521 213
AflII C'TTAA_G
Cuts at: 0 1728 1863
Size: 1728 135
AflIII A'CryG_T
Cuts at: 0 420 1863
Size: 420 1443
AgeI A'CCGG_T
Cuts at: 0 6 1863
Size: 6 1857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 933 1863
Size: 933 930
AlwNI CAG_nnn'CTG
Cuts at: 0 1689 1863
Size: 1689 174
ApoI r'AATT_y
Cuts at: 0 228 458 594 1012 1863
Size: 228 230 136 418 851
Fragments arranged by size:
851 418 230 228 136
AvaI C'yCGr_G
Cuts at: 0 811 1863
Size: 811 1052
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 388 421 1863
Size: 388 33 1442
Fragments arranged by size:
1442 388 33
BamHI G'GATC_C
Cuts at: 0 1120 1863
Size: 1120 743
BanI G'GyrC_C
Cuts at: 0 59 1541 1863
Size: 59 1482 322
Fragments arranged by size:
1482 322 59
BbeI G_GCGC'C
Cuts at: 0 63 1863
Size: 63 1800
BbsI GAAGACnn'nnnn_
Cuts at: 0 1400 1863
Size: 1400 463
BciVI GTATCCnnnnn_n'
Cuts at: 0 416 1863
Size: 416 1447
BglII A'GATC_T
Cuts at: 0 1630 1755 1863
Size: 1630 125 108
Fragments arranged by size:
1630 125 108
BlpI GC'TnA_GC
Cuts at: 0 1330 1863
Size: 1330 533
Bme1580I G_kGCm'C
Cuts at: 0 1546 1863
Size: 1546 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 993 1863
Size: 993 870
BmtI G_CTAG'C
Cuts at: 0 1415 1863
Size: 1415 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1540 1863
Size: 1540 323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1232 1863
Size: 691 541 631
Fragments arranged by size:
691 631 541
BsaI GGTCTCn'nnnn_
Cuts at: 0 528 1863
Size: 528 1335
BsaHI Gr'CG_yC
Cuts at: 0 60 349 1863
Size: 60 289 1514
Fragments arranged by size:
1514 289 60
BsaWI w'CCGG_w
Cuts at: 0 6 1442 1863
Size: 6 1436 421
Fragments arranged by size:
1436 421 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1577 1607 1863
Size: 1577 30 256
Fragments arranged by size:
1577 256 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 1215 1537 1863
Size: 98 1117 322 326
Fragments arranged by size:
1117 326 322 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1863
Size: 1014 849
BsmI GAATG_Cn'
Cuts at: 0 555 1824 1863
Size: 555 1269 39
Fragments arranged by size:
1269 555 39
Bsp1286I G_dGCh'C
Cuts at: 0 1546 1863
Size: 1546 317
BspEI T'CCGG_A
Cuts at: 0 1442 1863
Size: 1442 421
BspHI T'CATG_A
Cuts at: 0 1385 1863
Size: 1385 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1091 1863
Size: 1091 772
BsrDI GCAATG_nn'
Cuts at: 0 936 1262 1863
Size: 936 326 601
Fragments arranged by size:
936 601 326
BsrFI r'CCGG_y
Cuts at: 0 6 1863
Size: 6 1857
BsrGI T'GTAC_A
Cuts at: 0 417 1863
Size: 417 1446
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1568 1863
Size: 1568 295
BstBI TT'CG_AA
Cuts at: 0 291 1863
Size: 291 1572
BstYI r'GATC_y
Cuts at: 0 1120 1452 1630 1755 1863
Size: 1120 332 178 125 108
Fragments arranged by size:
1120 332 178 125 108
BtsI GCAGTG_nn'
Cuts at: 0 807 1324 1863
Size: 807 517 539
Fragments arranged by size:
807 539 517
DraIII CAC_nnn'GTG
Cuts at: 0 1568 1863
Size: 1568 295
EarI CTCTTCn'nnn_
Cuts at: 0 641 1863
Size: 641 1222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1129 1342 1540 1863
Size: 524 605 213 198 323
Fragments arranged by size:
605 524 323 213 198
EcoRI G'AATT_C
Cuts at: 0 458 1863
Size: 458 1405
HaeII r_GCGC'y
Cuts at: 0 63 1863
Size: 63 1800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 861 893 1064 1096 1280 1312
Size: 120 32 709 32 171 32 184 32
Cuts at: 1312 1437 1469 1863
Size: 125 32 394
Fragments arranged by size:
709 394 184 171 125 120 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 23 1863
Size: 23 1840
HindIII A'AGCT_T
Cuts at: 0 1250 1824 1863
Size: 1250 574 39
Fragments arranged by size:
1250 574 39
HpaI GTT'AAC
Cuts at: 0 23 1863
Size: 23 1840
KasI G'GCGC_C
Cuts at: 0 59 1863
Size: 59 1804
MslI CAynn'nnrTG
Cuts at: 0 941 1863
Size: 941 922
MspA1I CmG'CkG
Cuts at: 0 283 415 502 867 1689 1863
Size: 283 132 87 365 822 174
Fragments arranged by size:
822 365 283 174 132 87
NarI GG'CG_CC
Cuts at: 0 60 1863
Size: 60 1803
NheI G'CTAG_C
Cuts at: 0 1411 1863
Size: 1411 452
NspI r_CATG'y
Cuts at: 0 424 1667 1863
Size: 424 1243 196
Fragments arranged by size:
1243 424 196
PciI A'CATG_T
Cuts at: 0 420 1863
Size: 420 1443
PflMI CCAn_nnn'nTGG
Cuts at: 0 262 829 1863
Size: 262 567 1034
Fragments arranged by size:
1034 567 262
PfoI T'CCnGG_A
Cuts at: 0 441 1739 1863
Size: 441 1298 124
Fragments arranged by size:
1298 441 124
PstI C_TGCA'G
Cuts at: 0 1556 1863
Size: 1556 307
PvuII CAG'CTG
Cuts at: 0 283 415 502 867 1689 1863
Size: 283 132 87 365 822 174
Fragments arranged by size:
822 365 283 174 132 87
SexAI A'CCwGG_T
Cuts at: 0 1675 1863
Size: 1675 188
SfcI C'TryA_G
Cuts at: 0 110 410 1018 1207 1552 1863
Size: 110 300 608 189 345 311
Fragments arranged by size:
608 345 311 300 189 110
SfoI GGC'GCC
Cuts at: 0 61 1863
Size: 61 1802
SmlI C'TyrA_G
Cuts at: 0 706 1247 1728 1863
Size: 706 541 481 135
Fragments arranged by size:
706 541 481 135
SspI AAT'ATT
Cuts at: 0 646 1473 1863
Size: 646 827 390
Fragments arranged by size:
827 646 390
StuI AGG'CCT
Cuts at: 0 1213 1863
Size: 1213 650
StyI C'CwwG_G
Cuts at: 0 1533 1863
Size: 1533 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 1496 1863
Size: 115 1381 367
Fragments arranged by size:
1381 367 115
TatI w'GTAC_w
Cuts at: 0 417 1863
Size: 417 1446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1577 1609 1863
Size: 1577 32 254
Fragments arranged by size:
1577 254 32
XbaI T'CTAG_A
Cuts at: 0 426 1863
Size: 426 1437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1863
Size: 171 1692
Enzymes that do cut:
AcuI AflII AflIII AgeI AlfI AlwNI ApoI AvaI
BaeI BamHI BanI BbeI BbsI BciVI BglII BlpI
Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BsgI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstYI BtsI DraIII
EarI Eco57MI EcoRI HaeII Hin4I HincII HindIII HpaI
KasI MslI MspA1I NarI NheI NspI PciI PflMI
PfoI PstI PvuII SexAI SfcI SfoI SmlI SspI
StuI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BanII
BbvCI BcgI BclI BfrBI BglI BmgBI BplI Bpu10I
BsaAI BsaBI BseYI BsiEI BsiHKAI BsiWI BsmBI BsrBI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XhoI XmaI XmnI ZraI