Restriction map for
pFN21AE4169
(Linear) (Six-base) MAPSORT of: KIEE4169.seq Check: 4211 from: 1 to: 1863
KIEE4169.orf
With 182 enzymes: SgfI *
March 22, 2012 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1801 1863
Size: 1801 62
AccI GT'mk_AC
Cuts at: 0 1391 1863
Size: 1391 472
Acc65I G'GTAC_C
Cuts at: 0 1669 1863
Size: 1669 194
AfeI AGC'GCT
Cuts at: 0 1279 1863
Size: 1279 584
AhdI GACnn_n'nnGTC
Cuts at: 0 1311 1863
Size: 1311 552
AleI CACnn'nnGTG
Cuts at: 0 484 1863
Size: 484 1379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 1863
Size: 961 902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 1709 1741 1863
Size: 565 32 1112 32 122
Fragments arranged by size:
1112 565 122 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 255 1863
Size: 255 1608
AvaI C'yCGr_G
Cuts at: 0 742 1863
Size: 742 1121
BamHI G'GATC_C
Cuts at: 0 911 1863
Size: 911 952
BanI G'GyrC_C
Cuts at: 0 1479 1611 1669 1813 1863
Size: 1479 132 58 144 50
Fragments arranged by size:
1479 144 132 58 50
BanII G_rGCy'C
Cuts at: 0 68 530 1739 1863
Size: 68 462 1209 124
Fragments arranged by size:
1209 462 124 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 399 651 1074 1118 1863
Size: 399 252 423 44 745
Fragments arranged by size:
745 423 399 252 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1863
Size: 78 34 1751
Fragments arranged by size:
1751 78 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 910 1863
Size: 910 953
BclI T'GATC_A
Cuts at: 0 965 1863
Size: 965 898
BglI GCCn_nnn'nGGC
Cuts at: 0 844 1214 1863
Size: 844 370 649
Fragments arranged by size:
844 649 370
BlpI GC'TnA_GC
Cuts at: 0 632 1863
Size: 632 1231
Bme1580I G_kGCm'C
Cuts at: 0 738 859 1614 1863
Size: 738 121 755 249
Fragments arranged by size:
755 738 249 121
BmgBI CAC'GTC
Cuts at: 0 240 1053 1863
Size: 240 813 810
Fragments arranged by size:
813 810 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1246 1659 1863
Size: 1246 413 204
Fragments arranged by size:
1246 413 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 528 1112 1723 1736 1770 1863
Size: 241 287 584 611 13 34 93
Fragments arranged by size:
611 584 287 241 93 34 13
BsaAI yAC'GTr
Cuts at: 0 1245 1350 1395 1863
Size: 1245 105 45 468
Fragments arranged by size:
1245 468 105 45
BsaHI Gr'CG_yC
Cuts at: 0 617 1863
Size: 617 1246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 214 244 565 595 1696 1709
Size: 19 30 165 30 321 30 1101 13
Cuts at: 1709 1726 1739 1863
Size: 17 13 124
Fragments arranged by size:
1101 321 165 124 30 30 30 19
17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 238 586 892 901 988 1777 1780
Size: 40 198 348 306 9 87 789 3
Cuts at: 1780 1863
Size: 83
Fragments arranged by size:
789 348 306 198 87 83 40 9
3
BseYI C'CCAG_C
Cuts at: 0 6 737 1176 1546 1863
Size: 6 731 439 370 317
Fragments arranged by size:
731 439 370 317 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 241 322 781 1863
Size: 199 42 81 459 1082
Fragments arranged by size:
1082 459 199 81 42
BsiEI CG_ry'CG
Cuts at: 0 521 1863
Size: 521 1342
BsiHKAI G_wGCw'C
Cuts at: 0 530 1373 1739 1863
Size: 530 843 366 124
Fragments arranged by size:
843 530 366 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 418 445 1699 1863
Size: 418 27 1254 164
Fragments arranged by size:
1254 418 164 27
Bsp1286I G_dGCh'C
Cuts at: 0 68 530 738 859 1373 1614 1739 1863
Size: 68 462 208 121 514 241 125 124
Fragments arranged by size:
514 462 241 208 125 124 121 68
BspHI T'CATG_A
Cuts at: 0 962 1863
Size: 962 901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1801 1863
Size: 1801 62
BsrBI CCG'CTC
Cuts at: 0 1499 1863
Size: 1499 364
BsrGI T'GTAC_A
Cuts at: 0 266 1586 1863
Size: 266 1320 277
Fragments arranged by size:
1320 277 266
BssHII G'CGCG_C
Cuts at: 0 178 201 434 490 642 644 690 1863
Size: 178 23 233 56 152 2 46 1173
Fragments arranged by size:
1173 233 178 152 56 46 23 2
BssSI C'ACGA_G
Cuts at: 0 727 781 1863
Size: 727 54 1082
Fragments arranged by size:
1082 727 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1813 1863
Size: 1813 50
BstEII G'GTnAC_C
Cuts at: 0 1062 1551 1863
Size: 1062 489 312
Fragments arranged by size:
1062 489 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 1245 1863
Size: 399 846 618
Fragments arranged by size:
846 618 399
BstYI r'GATC_y
Cuts at: 0 335 911 951 1863
Size: 335 576 40 912
Fragments arranged by size:
912 576 335 40
BtgI C'CryG_G
Cuts at: 0 84 317 446 1863
Size: 84 233 129 1417
Fragments arranged by size:
1417 233 129 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 124 516 1863
Size: 124 392 1347
Fragments arranged by size:
1347 392 124
BtsI GCAGTG_nn'
Cuts at: 0 584 1419 1863
Size: 584 835 444
Fragments arranged by size:
835 584 444
DraIII CAC_nnn'GTG
Cuts at: 0 1368 1863
Size: 1368 495
EaeI y'GGCC_r
Cuts at: 0 152 401 518 1359 1527 1863
Size: 152 249 117 841 168 336
Fragments arranged by size:
841 336 249 168 152 117
EagI C'GGCC_G
Cuts at: 0 518 1863
Size: 518 1345
EarI CTCTTCn'nnn_
Cuts at: 0 1105 1863
Size: 1105 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1863
Size: 37 1826
EcoICRI GAG'CTC
Cuts at: 0 528 1737 1863
Size: 528 1209 126
Fragments arranged by size:
1209 528 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 528 1112 1723 1736 1770 1863
Size: 241 287 584 611 13 34 93
Fragments arranged by size:
611 584 287 241 93 34 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1863
Size: 223 1640
EcoO109I rG'GnC_Cy
Cuts at: 0 1038 1125 1863
Size: 1038 87 738
Fragments arranged by size:
1038 738 87
FspI TGC'GCA
Cuts at: 0 754 1863
Size: 754 1109
HaeII r_GCGC'y
Cuts at: 0 7 381 639 720 983 1281 1863
Size: 7 374 258 81 263 298 582
Fragments arranged by size:
582 374 298 263 258 81 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 1297 1329 1696 1728 1863
Size: 214 32 1051 32 367 32 135
Fragments arranged by size:
1051 367 214 135 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1673 1863
Size: 1673 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1055 1863
Size: 416 639 808
Fragments arranged by size:
808 639 416
MscI TGG'CCA
Cuts at: 0 1361 1863
Size: 1361 502
MslI CAynn'nnrTG
Cuts at: 0 358 484 961 1243 1320 1537 1863
Size: 358 126 477 282 77 217 326
Fragments arranged by size:
477 358 326 282 217 126 77
MspA1I CmG'CkG
Cuts at: 0 33 319 1191 1205 1341 1546 1863
Size: 33 286 872 14 136 205 317
Fragments arranged by size:
872 317 286 205 136 33 14
NcoI C'CATG_G
Cuts at: 0 84 1863
Size: 84 1779
NspI r_CATG'y
Cuts at: 0 78 942 1863
Size: 78 864 921
Fragments arranged by size:
921 864 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1863
Size: 144 1719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 565 597 1709 1741 1863
Size: 112 32 421 32 1112 32 122
Fragments arranged by size:
1112 421 122 112 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1125 1863
Size: 1038 87 738
Fragments arranged by size:
1038 738 87
PstI C_TGCA'G
Cuts at: 0 163 374 950 1151 1863
Size: 163 211 576 201 712
Fragments arranged by size:
712 576 211 201 163
PvuII CAG'CTG
Cuts at: 0 1546 1863
Size: 1546 317
SacI G_AGCT'C
Cuts at: 0 530 1739 1863
Size: 530 1209 124
Fragments arranged by size:
1209 530 124
SacII CC_GC'GG
Cuts at: 0 320 1863
Size: 320 1543
SbfI CC_TGCA'GG
Cuts at: 0 950 1863
Size: 950 913
SexAI A'CCwGG_T
Cuts at: 0 1034 1076 1863
Size: 1034 42 787
Fragments arranged by size:
1034 787 42
SfcI C'TryA_G
Cuts at: 0 159 370 946 1147 1863
Size: 159 211 576 201 716
Fragments arranged by size:
716 576 211 201 159
SnaBI TAC'GTA
Cuts at: 0 1395 1863
Size: 1395 468
StuI AGG'CCT
Cuts at: 0 1097 1624 1863
Size: 1097 527 239
Fragments arranged by size:
1097 527 239
StyI C'CwwG_G
Cuts at: 0 84 839 1067 1863
Size: 84 755 228 796
Fragments arranged by size:
796 755 228 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1281 1497 1863
Size: 1281 216 366
Fragments arranged by size:
1281 366 216
TatI w'GTAC_w
Cuts at: 0 266 1586 1863
Size: 266 1320 277
Fragments arranged by size:
1320 277 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 1156 1188 1863
Size: 314 32 810 32 675
Fragments arranged by size:
810 675 314 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1291 1387 1710 1863
Size: 1291 96 323 153
Fragments arranged by size:
1291 323 153 96
Enzymes that do cut:
AarI AccI Acc65I AfeI AhdI AleI AlfI AloI
AlwNI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI
NspI PflMI PpiI PpuMI PstI PvuII SacI SacII
SbfI SexAI SfcI SnaBI StuI StyI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AatII AclI AcuI AflII AflIII AgeI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaBI
BsaWI BsiWI BsmI BspEI BsrDI BsrFI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI
XmnI ZraI