Restriction map for
pFN21AE4167
(Linear) (Six-base) MAPSORT of: KIEE4167.seq Check: 5444 from: 1 to: 1857
KIEE4167.orf
With 182 enzymes: SgfI *
July 3, 2012 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1394 1857
Size: 1394 463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1857
Size: 1093 764
AflIII A'CryG_T
Cuts at: 0 362 935 1175 1857
Size: 362 573 240 682
Fragments arranged by size:
682 573 362 240
AleI CACnn'nnGTG
Cuts at: 0 50 1330 1857
Size: 50 1280 527
Fragments arranged by size:
1280 527 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1584 1857
Size: 1584 273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 1857
Size: 1633 32 192
Fragments arranged by size:
1633 192 32
ApaI G_GGCC'C
Cuts at: 0 1484 1582 1857
Size: 1484 98 275
Fragments arranged by size:
1484 275 98
ApaLI G'TGCA_C
Cuts at: 0 1179 1288 1857
Size: 1179 109 569
Fragments arranged by size:
1179 569 109
AvaI C'yCGr_G
Cuts at: 0 1575 1857
Size: 1575 282
BanI G'GyrC_C
Cuts at: 0 196 793 994 1618 1857
Size: 196 597 201 624 239
Fragments arranged by size:
624 597 239 201 196
BanII G_rGCy'C
Cuts at: 0 23 1484 1582 1814 1857
Size: 23 1461 98 232 43
Fragments arranged by size:
1461 232 98 43 23
BbeI G_GCGC'C
Cuts at: 0 1622 1857
Size: 1622 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 976 1010 1857
Size: 115 34 827 34 847
Fragments arranged by size:
847 827 115 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 957 1553 1577 1795 1799 1857
Size: 957 596 24 218 4 58
Fragments arranged by size:
957 596 218 58 24 4
Bme1580I G_kGCm'C
Cuts at: 0 1099 1183 1292 1484 1582 1857
Size: 1099 84 109 192 98 275
Fragments arranged by size:
1099 275 192 109 98 84
BmgBI CAC'GTC
Cuts at: 0 105 1857
Size: 105 1752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 361 391 1660 1711 1857
Size: 286 75 30 1269 51 146
Fragments arranged by size:
1269 286 146 75 51 30
Bpu10I CC'TnA_GC
Cuts at: 0 691 812 1624 1857
Size: 691 121 812 233
Fragments arranged by size:
812 691 233 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1857
Size: 1288 569
BsaI GGTCTCn'nnnn_
Cuts at: 0 726 1857
Size: 726 1131
BsaAI yAC'GTr
Cuts at: 0 1830 1857
Size: 1830 27
BsaHI Gr'CG_yC
Cuts at: 0 1619 1857
Size: 1619 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 334 364 688 718 1633 1663
Size: 115 30 189 30 324 30 915 30
Cuts at: 1663 1819 1849 1857
Size: 156 30 8
Fragments arranged by size:
915 324 189 156 115 30 30 30
30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 466 478 481 484 493 496 886
Size: 418 48 12 3 3 9 3 390
Cuts at: 886 889 898 1669 1857
Size: 3 9 771 188
Fragments arranged by size:
771 418 390 188 48 12 9 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 803 1530 1582 1857
Size: 803 727 52 275
Fragments arranged by size:
803 727 275 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 139 1372 1857
Size: 84 55 1233 485
Fragments arranged by size:
1233 485 84 55
BsiEI CG_ry'CG
Cuts at: 0 1440 1857
Size: 1440 417
BsiHKAI G_wGCw'C
Cuts at: 0 23 110 1183 1292 1857
Size: 23 87 1073 109 565
Fragments arranged by size:
1073 565 109 87 23
BsmI GAATG_Cn'
Cuts at: 0 29 1857
Size: 29 1828
Bsp1286I G_dGCh'C
Cuts at: 0 23 110 1099 1183 1292 1484 1582 1814
Size: 23 87 989 84 109 192 98 232
Cuts at: 1814 1857
Size: 43
Fragments arranged by size:
989 232 192 109 98 87 84 43
23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 956 1394 1857
Size: 956 438 463
Fragments arranged by size:
956 463 438
BsrBI CCG'CTC
Cuts at: 0 146 1251 1644 1857
Size: 146 1105 393 213
Fragments arranged by size:
1105 393 213 146
BsrDI GCAATG_nn'
Cuts at: 0 312 1857
Size: 312 1545
BsrFI r'CCGG_y
Cuts at: 0 193 1789 1798 1816 1857
Size: 193 1596 9 18 41
Fragments arranged by size:
1596 193 41 18 9
BssHII G'CGCG_C
Cuts at: 0 1680 1682 1857
Size: 1680 2 175
Fragments arranged by size:
1680 175 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1187 1271 1857
Size: 1187 84 586
Fragments arranged by size:
1187 586 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 776 1026 1857
Size: 339 437 250 831
Fragments arranged by size:
831 437 339 250
BstYI r'GATC_y
Cuts at: 0 344 840 1857
Size: 344 496 1017
Fragments arranged by size:
1017 496 344
Bsu36I CC'TnA_GG
Cuts at: 0 245 1857
Size: 245 1612
BtgI C'CryG_G
Cuts at: 0 821 1138 1857
Size: 821 317 719
Fragments arranged by size:
821 719 317
BtsI GCAGTG_nn'
Cuts at: 0 57 1099 1269 1857
Size: 57 1042 170 588
Fragments arranged by size:
1042 588 170 57
EaeI y'GGCC_r
Cuts at: 0 2 648 778 1437 1545 1733 1796 1857
Size: 2 646 130 659 108 188 63 61
Fragments arranged by size:
659 646 188 130 108 63 61 2
EagI C'GGCC_G
Cuts at: 0 1437 1857
Size: 1437 420
EarI CTCTTCn'nnn_
Cuts at: 0 455 470 1042 1055 1480 1857
Size: 455 15 572 13 425 377
Fragments arranged by size:
572 455 425 377 15 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 994 1857
Size: 994 863
EcoICRI GAG'CTC
Cuts at: 0 21 1857
Size: 21 1836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 361 391 1093 1660 1711 1857
Size: 286 75 30 702 567 51 146
Fragments arranged by size:
702 567 286 146 75 51 30
EcoO109I rG'GnC_Cy
Cuts at: 0 57 958 967 1151 1579 1713 1857
Size: 57 901 9 184 428 134 144
Fragments arranged by size:
901 428 184 144 134 57 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1527 1857
Size: 1527 330
FseI GG_CCGG'CC
Cuts at: 0 1802 1857
Size: 1802 55
FspI TGC'GCA
Cuts at: 0 1318 1857
Size: 1318 539
FspAI rTGC'GCAy
Cuts at: 0 1318 1857
Size: 1318 539
HaeII r_GCGC'y
Cuts at: 0 1407 1612 1622 1857
Size: 1407 205 10 235
Fragments arranged by size:
1407 235 205 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 1857
Size: 564 32 1261
Fragments arranged by size:
1261 564 32
HindIII A'AGCT_T
Cuts at: 0 181 1857
Size: 181 1676
KasI G'GCGC_C
Cuts at: 0 1618 1857
Size: 1618 239
MscI TGG'CCA
Cuts at: 0 4 650 780 1857
Size: 4 646 130 1077
Fragments arranged by size:
1077 646 130 4
MslI CAynn'nnrTG
Cuts at: 0 50 1176 1330 1857
Size: 50 1126 154 527
Fragments arranged by size:
1126 527 154 50
MspA1I CmG'CkG
Cuts at: 0 301 551 807 1140 1564 1689 1770 1857
Size: 301 250 256 333 424 125 81 87
Fragments arranged by size:
424 333 301 256 250 125 87 81
NaeI GCC'GGC
Cuts at: 0 195 1791 1800 1818 1857
Size: 195 1596 9 18 39
Fragments arranged by size:
1596 195 39 18 9
NarI GG'CG_CC
Cuts at: 0 1619 1857
Size: 1619 238
NcoI C'CATG_G
Cuts at: 0 821 1857
Size: 821 1036
NgoMIV G'CCGG_C
Cuts at: 0 193 1789 1798 1816 1857
Size: 193 1596 9 18 41
Fragments arranged by size:
1596 193 41 18 9
NotI GC'GGCC_GC
Cuts at: 0 1437 1857
Size: 1437 420
NspI r_CATG'y
Cuts at: 0 939 1179 1233 1317 1857
Size: 939 240 54 84 540
Fragments arranged by size:
939 540 240 84 54
PasI CC'CwG_GG
Cuts at: 0 611 1807 1857
Size: 611 1196 50
Fragments arranged by size:
1196 611 50
PciI A'CATG_T
Cuts at: 0 935 1175 1857
Size: 935 240 682
Fragments arranged by size:
935 682 240
PfoI T'CCnGG_A
Cuts at: 0 263 665 1857
Size: 263 402 1192
Fragments arranged by size:
1192 402 263
PmlI CAC'GTG
Cuts at: 0 1830 1857
Size: 1830 27
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 1857
Size: 1633 32 192
Fragments arranged by size:
1633 192 32
PpuMI rG'GwC_Cy
Cuts at: 0 57 967 1151 1857
Size: 57 910 184 706
Fragments arranged by size:
910 706 184 57
PspOMI G'GGCC_C
Cuts at: 0 1480 1578 1857
Size: 1480 98 279
Fragments arranged by size:
1480 279 98
PstI C_TGCA'G
Cuts at: 0 166 1857
Size: 166 1691
PvuII CAG'CTG
Cuts at: 0 551 807 1564 1689 1857
Size: 551 256 757 125 168
Fragments arranged by size:
757 551 256 168 125
SacI G_AGCT'C
Cuts at: 0 23 1857
Size: 23 1834
SacII CC_GC'GG
Cuts at: 0 1141 1857
Size: 1141 716
SanDI GG'GwC_CC
Cuts at: 0 967 1857
Size: 967 890
SapI GCTCTTCn'nnn_
Cuts at: 0 1055 1480 1857
Size: 1055 425 377
Fragments arranged by size:
1055 425 377
ScaI AGT'ACT
Cuts at: 0 172 1857
Size: 172 1685
SfcI C'TryA_G
Cuts at: 0 162 1857
Size: 162 1695
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1799 1857
Size: 1799 58
SfoI GGC'GCC
Cuts at: 0 1620 1857
Size: 1620 237
SmaI CCC'GGG
Cuts at: 0 1577 1857
Size: 1577 280
SmlI C'TyrA_G
Cuts at: 0 1303 1857
Size: 1303 554
StuI AGG'CCT
Cuts at: 0 595 1034 1084 1857
Size: 595 439 50 773
Fragments arranged by size:
773 595 439 50
StyI C'CwwG_G
Cuts at: 0 710 821 1079 1613 1780 1857
Size: 710 111 258 534 167 77
Fragments arranged by size:
710 534 258 167 111 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1036 1857
Size: 1036 821
TatI w'GTAC_w
Cuts at: 0 170 1857
Size: 170 1687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 1819 1851 1857
Size: 334 32 1453 32 6
Fragments arranged by size:
1453 334 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 0 235 1857
Size: 235 1622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1599 1857
Size: 1599 258
XmaI C'CCGG_G
Cuts at: 0 1575 1857
Size: 1575 282
XmnI GAAnn'nnTTC
Cuts at: 0 184 1672 1857
Size: 184 1488 185
Fragments arranged by size:
1488 185 184
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI ApaI ApaLI
AvaI BanI BanII BbeI BcgI BglI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI FseI FspI FspAI HaeII Hin4I HindIII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI ScaI SfcI
SfiI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BsaBI BsaWI BsiWI BsmBI BspEI BspHI
BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoNI EcoRI EcoRV HincII HpaI
KpnI MfeI MluI MmeI NdeI NheI NruI NsiI
PacI PflMI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SbfI SexAI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI ZraI