Restriction map for
pFN21AE4164
(Linear) (Six-base) MAPSORT of: KIEE4164.seq Check: 65 from: 1 to: 1824
KIEE4164.orf
With 182 enzymes: SgfI *
March 22, 2012 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1609 1824
Size: 1609 215
AccI GT'mk_AC
Cuts at: 0 1700 1824
Size: 1700 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 724 1824
Size: 638 86 1100
Fragments arranged by size:
1100 638 86
AflII C'TTAA_G
Cuts at: 0 102 1824
Size: 102 1722
AflIII A'CryG_T
Cuts at: 0 34 1824
Size: 34 1790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1356 1824
Size: 1356 468
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1824
Size: 736 32 1056
Fragments arranged by size:
1056 736 32
ApoI r'AATT_y
Cuts at: 0 44 117 567 1643 1759 1824
Size: 44 73 450 1076 116 65
Fragments arranged by size:
1076 450 116 73 65 44
AseI AT'TA_AT
Cuts at: 0 434 1489 1824
Size: 434 1055 335
Fragments arranged by size:
1055 434 335
AvaI C'yCGr_G
Cuts at: 0 1232 1824
Size: 1232 592
BamHI G'GATC_C
Cuts at: 0 1228 1824
Size: 1228 596
BclI T'GATC_A
Cuts at: 0 498 1098 1437 1824
Size: 498 600 339 387
Fragments arranged by size:
600 498 387 339
BfrBI ATG'CAT
Cuts at: 0 505 1173 1824
Size: 505 668 651
Fragments arranged by size:
668 651 505
BsaI GGTCTCn'nnnn_
Cuts at: 0 1790 1824
Size: 1790 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 1824
Size: 745 1079
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1714 1824
Size: 1625 89 110
Fragments arranged by size:
1625 110 89
BspHI T'CATG_A
Cuts at: 0 1086 1824
Size: 1086 738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1609 1686 1824
Size: 1609 77 138
Fragments arranged by size:
1609 138 77
BsrGI T'GTAC_A
Cuts at: 0 516 1513 1824
Size: 516 997 311
Fragments arranged by size:
997 516 311
BstYI r'GATC_y
Cuts at: 0 988 1228 1591 1824
Size: 988 240 363 233
Fragments arranged by size:
988 363 240 233
BtgI C'CryG_G
Cuts at: 0 1801 1824
Size: 1801 23
BtsI GCAGTG_nn'
Cuts at: 0 213 417 1824
Size: 213 204 1407
Fragments arranged by size:
1407 213 204
DraI TTT'AAA
Cuts at: 0 543 1419 1824
Size: 543 876 405
Fragments arranged by size:
876 543 405
EarI CTCTTCn'nnn_
Cuts at: 0 755 844 1824
Size: 755 89 980
Fragments arranged by size:
980 755 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 724 1824
Size: 638 86 1100
Fragments arranged by size:
1100 638 86
EcoRI G'AATT_C
Cuts at: 0 44 1824
Size: 44 1780
EcoRV GAT'ATC
Cuts at: 0 1292 1824
Size: 1292 532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 661 1824
Size: 661 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 83 115 702 734 1190 1222 1246 1278
Size: 83 32 587 32 456 32 24 32
Cuts at: 1278 1429 1461 1824
Size: 151 32 363
Fragments arranged by size:
587 456 363 151 83 32 32 32
32 32 24
HindIII A'AGCT_T
Cuts at: 0 404 1824
Size: 404 1420
MfeI C'AATT_G
Cuts at: 0 444 1625 1824
Size: 444 1181 199
Fragments arranged by size:
1181 444 199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1824
Size: 631 1193
MslI CAynn'nnrTG
Cuts at: 0 188 799 1824
Size: 188 611 1025
Fragments arranged by size:
1025 611 188
NcoI C'CATG_G
Cuts at: 0 1801 1824
Size: 1801 23
NdeI CA'TA_TG
Cuts at: 0 12 1824
Size: 12 1812
NsiI A_TGCA'T
Cuts at: 0 507 1175 1824
Size: 507 668 649
Fragments arranged by size:
668 649 507
NspI r_CATG'y
Cuts at: 0 38 206 1343 1824
Size: 38 168 1137 481
Fragments arranged by size:
1137 481 168 38
PacI TTA_AT'TAA
Cuts at: 0 1493 1824
Size: 1493 331
PciI A'CATG_T
Cuts at: 0 34 1824
Size: 34 1790
PflMI CCAn_nnn'nTGG
Cuts at: 0 1286 1824
Size: 1286 538
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1343 1375 1824
Size: 1158 32 153 32 449
Fragments arranged by size:
1158 449 153 32 32
ScaI AGT'ACT
Cuts at: 0 578 1824
Size: 578 1246
SexAI A'CCwGG_T
Cuts at: 0 1105 1824
Size: 1105 719
SfcI C'TryA_G
Cuts at: 0 112 580 1824
Size: 112 468 1244
Fragments arranged by size:
1244 468 112
SmaI CCC'GGG
Cuts at: 0 1234 1824
Size: 1234 590
SmlI C'TyrA_G
Cuts at: 0 102 1824
Size: 102 1722
SpeI A'CTAG_T
Cuts at: 0 1452 1824
Size: 1452 372
SphI G_CATG'C
Cuts at: 0 206 1824
Size: 206 1618
SspI AAT'ATT
Cuts at: 0 1019 1824
Size: 1019 805
StyI C'CwwG_G
Cuts at: 0 287 1801 1824
Size: 287 1514 23
Fragments arranged by size:
1514 287 23
SwaI ATTT'AAAT
Cuts at: 0 543 1824
Size: 543 1281
TatI w'GTAC_w
Cuts at: 0 108 516 576 1513 1824
Size: 108 408 60 937 311
Fragments arranged by size:
937 408 311 108 60
XmaI C'CCGG_G
Cuts at: 0 1232 1824
Size: 1232 592
XmnI GAAnn'nnTTC
Cuts at: 0 1018 1824
Size: 1018 806
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AloI ApoI
AseI AvaI BamHI BclI BfrBI BsaI BseRI BsgI
BspHI BspMI BsrGI BstYI BtgI BtsI DraI EarI
Eco57MI EcoRI EcoRV FalI Hin4I HindIII MfeI MmeI
MslI NcoI NdeI NsiI NspI PacI PciI PflMI
PsrI ScaI SexAI SfcI SmaI SmlI SpeI SphI
SspI StyI SwaI TatI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlwNI
ApaI ApaLI AscI AsiSI AvrII BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SnaBI SrfI StuI TaqII
TstI Tth111I XbaI XcmI XhoI ZraI