Restriction map for
pFN21AE4154
(Linear) (Six-base) MAPSORT of: KIEE4154.seq Check: 9918 from: 1 to: 1767
KIEE4154.orf
With 182 enzymes: SgfI *
March 22, 2012 17:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 56 843 1767
Size: 56 787 924
Fragments arranged by size:
924 787 56
AatII G_ACGT'C
Cuts at: 0 98 199 907 1767
Size: 98 101 708 860
Fragments arranged by size:
860 708 101 98
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1767
Size: 1168 599
AleI CACnn'nnGTG
Cuts at: 0 1096 1767
Size: 1096 671
ApaI G_GGCC'C
Cuts at: 0 1051 1209 1535 1598 1767
Size: 1051 158 326 63 169
Fragments arranged by size:
1051 326 169 158 63
ApaLI G'TGCA_C
Cuts at: 0 1500 1767
Size: 1500 267
BanI G'GyrC_C
Cuts at: 0 44 439 885 1487 1767
Size: 44 395 446 602 280
Fragments arranged by size:
602 446 395 280 44
BanII G_rGCy'C
Cuts at: 0 62 608 998 1051 1209 1535 1567 1598
Size: 62 546 390 53 158 326 32 31
Cuts at: 1598 1767
Size: 169
Fragments arranged by size:
546 390 326 169 158 62 53 32
31
BbeI G_GCGC'C
Cuts at: 0 443 1491 1767
Size: 443 1048 276
Fragments arranged by size:
1048 443 276
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 199 1437 1712 1767
Size: 114 85 1238 275 55
Fragments arranged by size:
1238 275 114 85 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 1016 1767
Size: 982 34 751
Fragments arranged by size:
982 751 34
BclI T'GATC_A
Cuts at: 0 1238 1767
Size: 1238 529
BglI GCCn_nnn'nGGC
Cuts at: 0 484 648 1767
Size: 484 164 1119
Fragments arranged by size:
1119 484 164
BglII A'GATC_T
Cuts at: 0 1346 1767
Size: 1346 421
Bme1580I G_kGCm'C
Cuts at: 0 1051 1209 1504 1535 1598 1767
Size: 1051 158 295 31 63 169
Fragments arranged by size:
1051 295 169 158 63 31
BmgBI CAC'GTC
Cuts at: 0 660 1158 1767
Size: 660 498 609
Fragments arranged by size:
660 609 498
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1725 1767
Size: 1725 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1467 1553 1624 1767
Size: 674 793 86 71 143
Fragments arranged by size:
793 674 143 86 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1767
Size: 1282 485
BsaI GGTCTCn'nnnn_
Cuts at: 0 823 1024 1767
Size: 823 201 743
Fragments arranged by size:
823 743 201
BsaHI Gr'CG_yC
Cuts at: 0 83 95 196 407 440 904 1076 1488
Size: 83 12 101 211 33 464 172 412
Cuts at: 1488 1767
Size: 279
Fragments arranged by size:
464 412 279 211 172 101 83 33
12
BsaWI w'CCGG_w
Cuts at: 0 541 1767
Size: 541 1226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 507 995 1027 1043 1651 1767
Size: 507 488 32 16 608 116
Fragments arranged by size:
608 507 488 116 32 16
BseYI C'CCAG_C
Cuts at: 0 147 1161 1641 1767
Size: 147 1014 480 126
Fragments arranged by size:
1014 480 147 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 767 1455 1767
Size: 208 559 688 312
Fragments arranged by size:
688 559 312 208
BsiEI CG_ry'CG
Cuts at: 0 479 1720 1767
Size: 479 1241 47
Fragments arranged by size:
1241 479 47
BsiHKAI G_wGCw'C
Cuts at: 0 62 998 1379 1504 1767
Size: 62 936 381 125 263
Fragments arranged by size:
936 381 263 125 62
BsmBI CGTCTCn'nnnn_
Cuts at: 0 650 710 1767
Size: 650 60 1057
Fragments arranged by size:
1057 650 60
Bsp1286I G_dGCh'C
Cuts at: 0 62 608 998 1051 1209 1379 1504 1535
Size: 62 546 390 53 158 170 125 31
Cuts at: 1535 1567 1598 1767
Size: 32 31 169
Fragments arranged by size:
546 390 170 169 158 125 62 53
32 31 31
BspEI T'CCGG_A
Cuts at: 0 541 1767
Size: 541 1226
BspHI T'CATG_A
Cuts at: 0 1121 1767
Size: 1121 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 684 843 1115 1202 1767
Size: 56 628 159 272 87 565
Fragments arranged by size:
628 565 272 159 87 56
BsrBI CCG'CTC
Cuts at: 0 1329 1767
Size: 1329 438
BsrFI r'CCGG_y
Cuts at: 0 41 1767
Size: 41 1726
BssHII G'CGCG_C
Cuts at: 0 1110 1767
Size: 1110 657
BssSI C'ACGA_G
Cuts at: 0 1622 1767
Size: 1622 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 1433 1767
Size: 51 1382 334
Fragments arranged by size:
1382 334 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1044 1098 1767
Size: 1044 54 669
Fragments arranged by size:
1044 669 54
BstYI r'GATC_y
Cuts at: 0 1346 1767
Size: 1346 421
Bsu36I CC'TnA_GG
Cuts at: 0 1613 1670 1767
Size: 1613 57 97
Fragments arranged by size:
1613 97 57
BtgI C'CryG_G
Cuts at: 0 209 505 797 1094 1491 1518 1542 1714
Size: 209 296 292 297 397 27 24 172
Cuts at: 1714 1767
Size: 53
Fragments arranged by size:
397 297 296 292 209 172 53 27
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 1767
Size: 540 1227
DraIII CAC_nnn'GTG
Cuts at: 0 1497 1767
Size: 1497 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 949 1767
Size: 949 818
EaeI y'GGCC_r
Cuts at: 0 476 485 551 913 1100 1277 1515 1526
Size: 476 9 66 362 187 177 238 11
Cuts at: 1526 1717 1767
Size: 191 50
Fragments arranged by size:
476 362 238 191 187 177 66 50
11 9
EagI C'GGCC_G
Cuts at: 0 476 1717 1767
Size: 476 1241 50
Fragments arranged by size:
1241 476 50
EarI CTCTTCn'nnn_
Cuts at: 0 683 1767
Size: 683 1084
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1443 1565 1648 1702 1767
Size: 1443 122 83 54 65
Fragments arranged by size:
1443 122 83 65 54
EcoICRI GAG'CTC
Cuts at: 0 60 996 1767
Size: 60 936 771
Fragments arranged by size:
936 771 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1168 1467 1553 1624 1767
Size: 674 494 299 86 71 143
Fragments arranged by size:
674 494 299 143 86 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1084 1767
Size: 1084 683
EcoO109I rG'GnC_Cy
Cuts at: 0 1048 1205 1532 1595 1608 1617 1659 1738
Size: 1048 157 327 63 13 9 42 79
Cuts at: 1738 1767
Size: 29
Fragments arranged by size:
1048 327 157 79 63 42 29 13
9
FspI TGC'GCA
Cuts at: 0 679 1767
Size: 679 1088
HaeII r_GCGC'y
Cuts at: 0 289 385 443 1120 1389 1491 1579 1767
Size: 289 96 58 677 269 102 88 188
Fragments arranged by size:
677 289 269 188 102 96 88 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 1159 1191 1767
Size: 301 32 826 32 576
Fragments arranged by size:
826 576 301 32 32
KasI G'GCGC_C
Cuts at: 0 439 1487 1767
Size: 439 1048 280
Fragments arranged by size:
1048 439 280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 383 995 1767
Size: 179 204 612 772
Fragments arranged by size:
772 612 204 179
MscI TGG'CCA
Cuts at: 0 1102 1767
Size: 1102 665
MslI CAynn'nnrTG
Cuts at: 0 310 1096 1767
Size: 310 786 671
Fragments arranged by size:
786 671 310
MspA1I CmG'CkG
Cuts at: 0 563 1096 1520 1716 1767
Size: 563 533 424 196 51
Fragments arranged by size:
563 533 424 196 51
NarI GG'CG_CC
Cuts at: 0 440 1488 1767
Size: 440 1048 279
Fragments arranged by size:
1048 440 279
NcoI C'CATG_G
Cuts at: 0 1542 1767
Size: 1542 225
NotI GC'GGCC_GC
Cuts at: 0 1717 1767
Size: 1717 50
NspI r_CATG'y
Cuts at: 0 1311 1767
Size: 1311 456
PasI CC'CwG_GG
Cuts at: 0 240 1767
Size: 240 1527
PflMI CCAn_nnn'nTGG
Cuts at: 0 1395 1767
Size: 1395 372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 1564 1596 1767
Size: 748 32 784 32 171
Fragments arranged by size:
784 748 171 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1608 1617 1767
Size: 1608 9 150
Fragments arranged by size:
1608 150 9
PspOMI G'GGCC_C
Cuts at: 0 1047 1205 1531 1594 1767
Size: 1047 158 326 63 173
Fragments arranged by size:
1047 326 173 158 63
PstI C_TGCA'G
Cuts at: 0 338 1270 1767
Size: 338 932 497
Fragments arranged by size:
932 497 338
PvuII CAG'CTG
Cuts at: 0 563 1767
Size: 563 1204
SacI G_AGCT'C
Cuts at: 0 62 998 1767
Size: 62 936 769
Fragments arranged by size:
936 769 62
SacII CC_GC'GG
Cuts at: 0 1097 1521 1717 1767
Size: 1097 424 196 50
Fragments arranged by size:
1097 424 196 50
SapI GCTCTTCn'nnn_
Cuts at: 0 683 1767
Size: 683 1084
SfcI C'TryA_G
Cuts at: 0 334 1266 1767
Size: 334 932 501
Fragments arranged by size:
932 501 334
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 484 1767
Size: 484 1283
SfoI GGC'GCC
Cuts at: 0 441 1489 1767
Size: 441 1048 278
Fragments arranged by size:
1048 441 278
SmlI C'TyrA_G
Cuts at: 0 1297 1767
Size: 1297 470
StyI C'CwwG_G
Cuts at: 0 608 737 1542 1767
Size: 608 129 805 225
Fragments arranged by size:
805 608 225 129
TatI w'GTAC_w
Cuts at: 0 269 750 1767
Size: 269 481 1017
Fragments arranged by size:
1017 481 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 1767
Size: 1201 32 534
Fragments arranged by size:
1201 534 32
Tth111I GACn'n_nGTC
Cuts at: 0 229 1767
Size: 229 1538
XmnI GAAnn'nnTTC
Cuts at: 0 760 1228 1249 1360 1767
Size: 760 468 21 111 407
Fragments arranged by size:
760 468 407 111 21
ZraI GAC'GTC
Cuts at: 0 96 197 905 1767
Size: 96 101 708 862
Fragments arranged by size:
862 708 101 96
Enzymes that do cut:
AarI AatII AcuI AleI ApaI ApaLI BanI BanII
BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI
BplI BpmI BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NarI NcoI NotI NspI
PasI PflMI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SapI SfcI SfiI SfoI SmlI StyI TatI
TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BciVI BfrBI BlpI BmrI
BmtI Bpu10I BsaAI BsaBI BsaXI BsiWI BsmI BsrDI
BsrGI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI
NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XcmI XhoI XmaI