Restriction map for
pFN21AE4151
(Linear) (Six-base) MAPSORT of: KIEE4151.seq Check: 2713 from: 1 to: 1758
KIEE4151.orf
With 182 enzymes: SgfI *
July 3, 2012 18:40 ..
AccI GT'mk_AC
Cuts at: 0 1013 1758
Size: 1013 745
Acc65I G'GTAC_C
Cuts at: 0 803 1758
Size: 803 955
AclI AA'CG_TT
Cuts at: 0 124 1758
Size: 124 1634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1758
Size: 542 1216
AfeI AGC'GCT
Cuts at: 0 1258 1758
Size: 1258 500
AflII C'TTAA_G
Cuts at: 0 726 778 1758
Size: 726 52 980
Fragments arranged by size:
980 726 52
AlwNI CAG_nnn'CTG
Cuts at: 0 10 1758
Size: 10 1748
ApoI r'AATT_y
Cuts at: 0 298 317 541 720 1148 1758
Size: 298 19 224 179 428 610
Fragments arranged by size:
610 428 298 224 179 19
AvaI C'yCGr_G
Cuts at: 0 59 1112 1758
Size: 59 1053 646
Fragments arranged by size:
1053 646 59
BanI G'GyrC_C
Cuts at: 0 803 1758
Size: 803 955
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 1758
Size: 43 1715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1040 1074 1758
Size: 1040 34 684
Fragments arranged by size:
1040 684 34
BmgBI CAC'GTC
Cuts at: 0 135 1758
Size: 135 1623
BmrI ACTGGGnnnn_n'
Cuts at: 0 1105 1758
Size: 1105 653
BmtI G_CTAG'C
Cuts at: 0 457 1758
Size: 457 1301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1181 1758
Size: 803 378 577
Fragments arranged by size:
803 577 378
BsaI GGTCTCn'nnnn_
Cuts at: 0 1536 1738 1758
Size: 1536 202 20
Fragments arranged by size:
1536 202 20
BsaHI Gr'CG_yC
Cuts at: 0 95 1758
Size: 95 1663
BseYI C'CCAG_C
Cuts at: 0 1240 1253 1758
Size: 1240 13 505
Fragments arranged by size:
1240 505 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1589 1758
Size: 1589 169
BsmI GAATG_Cn'
Cuts at: 0 778 1758
Size: 778 980
BspHI T'CATG_A
Cuts at: 0 98 1758
Size: 98 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 174 1758
Size: 174 1584
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1135 1758
Size: 1135 623
BstBI TT'CG_AA
Cuts at: 0 1090 1758
Size: 1090 668
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1544 1758
Size: 1544 214
BstZ17I GTA'TAC
Cuts at: 0 1014 1758
Size: 1014 744
BtgI C'CryG_G
Cuts at: 0 1266 1543 1758
Size: 1266 277 215
Fragments arranged by size:
1266 277 215
EarI CTCTTCn'nnn_
Cuts at: 0 16 972 1736 1758
Size: 16 956 764 22
Fragments arranged by size:
956 764 22 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 803 1181 1758
Size: 542 261 378 577
Fragments arranged by size:
577 542 378 261
EcoO109I rG'GnC_Cy
Cuts at: 0 1099 1758
Size: 1099 659
EcoRV GAT'ATC
Cuts at: 0 684 1758
Size: 684 1074
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 741 1758
Size: 741 1017
HaeII r_GCGC'y
Cuts at: 0 1260 1758
Size: 1260 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 792 824 1619 1651 1758
Size: 153 32 607 32 795 32 107
Fragments arranged by size:
795 607 153 107 32 32 32
KpnI G_GTAC'C
Cuts at: 0 807 1758
Size: 807 951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1129 1758
Size: 332 797 629
Fragments arranged by size:
797 629 332
MspA1I CmG'CkG
Cuts at: 0 959 1758
Size: 959 799
NcoI C'CATG_G
Cuts at: 0 1266 1543 1758
Size: 1266 277 215
Fragments arranged by size:
1266 277 215
NheI G'CTAG_C
Cuts at: 0 453 1758
Size: 453 1305
NspI r_CATG'y
Cuts at: 0 111 231 992 1133 1248 1758
Size: 111 120 761 141 115 510
Fragments arranged by size:
761 510 141 120 115 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 1272 1758
Size: 1272 486
PfoI T'CCnGG_A
Cuts at: 0 149 1758
Size: 149 1609
PpuMI rG'GwC_Cy
Cuts at: 0 1099 1758
Size: 1099 659
PstI C_TGCA'G
Cuts at: 0 192 495 633 1140 1264 1758
Size: 192 303 138 507 124 494
Fragments arranged by size:
507 494 303 192 138 124
SapI GCTCTTCn'nnn_
Cuts at: 0 16 1758
Size: 16 1742
SfcI C'TryA_G
Cuts at: 0 188 491 629 1136 1260 1583 1758
Size: 188 303 138 507 124 323 175
Fragments arranged by size:
507 323 303 188 175 138 124
SmlI C'TyrA_G
Cuts at: 0 726 778 1112 1758
Size: 726 52 334 646
Fragments arranged by size:
726 646 334 52
SphI G_CATG'C
Cuts at: 0 992 1248 1758
Size: 992 256 510
Fragments arranged by size:
992 510 256
SspI AAT'ATT
Cuts at: 0 1498 1758
Size: 1498 260
StyI C'CwwG_G
Cuts at: 0 1266 1543 1758
Size: 1266 277 215
Fragments arranged by size:
1266 277 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 842 1447 1758
Size: 55 787 605 311
Fragments arranged by size:
787 605 311 55
XbaI T'CTAG_A
Cuts at: 0 465 1758
Size: 465 1293
XhoI C'TCGA_G
Cuts at: 0 1112 1758
Size: 1112 646
XmnI GAAnn'nnTTC
Cuts at: 0 545 1758
Size: 545 1213
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AlwNI ApoI
AvaI BanI BbsI BcgI BmgBI BmrI BmtI BpmI
BsaI BsaHI BseYI BsgI BsmI BspHI BspMI BstAPI
BstBI BstXI BstZ17I BtgI EarI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I KpnI MmeI MspA1I NcoI NheI
NspI PflMI PfoI PpuMI PstI SapI SfcI SmlI
SphI SspI StyI TaqII XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AflIII AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TatI
TstI Tth111I XcmI XmaI ZraI