(Linear) (Six-base) MAPSORT of: KIEE4148.seq  Check: 4307  from: 1  to: 1749



KIEE4148.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:39  ..

AclI AA'CG_TT

Cuts at:      0    1081    1749 
   Size:       1081     668
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     929     974    1749 
   Size:        929      45     775

  Fragments arranged by size: 

                 929     775      45
 
AflII C'TTAA_G

Cuts at:      0      78     136    1749 
   Size:         78      58    1613

  Fragments arranged by size: 

                1613      78      58
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1709    1749 
   Size:       1709      40
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46    1369    1749 
   Size:         46    1323     380

  Fragments arranged by size: 

                1323     380      46
 
ApoI r'AATT_y

Cuts at:      0     687     811    1066    1632    1749 
   Size:        687     124     255     566     117

  Fragments arranged by size: 

                 687     566     255     124     117
 
AseI AT'TA_AT

Cuts at:      0     175    1749 
   Size:        175    1574
 
AvaI C'yCGr_G

Cuts at:      0    1145    1749 
   Size:       1145     604
 
BanII G_rGCy'C

Cuts at:      0    1133    1749 
   Size:       1133     616
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     827    1749 
   Size:        827     922
 
Bme1580I G_kGCm'C

Cuts at:      0     450    1749 
   Size:        450    1299
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     982    1749 
   Size:        982     767
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1216    1749 
   Size:       1216     533
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242    1715    1749 
   Size:        242    1473      34

  Fragments arranged by size: 

                1473     242      34
 
BsmI GAATG_Cn'

Cuts at:      0     593     662    1671    1749 
   Size:        593      69    1009      78

  Fragments arranged by size: 

                1009     593      78      69
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     926    1749 
   Size:        926     823
 
Bsp1286I G_dGCh'C

Cuts at:      0     450    1133    1749 
   Size:        450     683     616

  Fragments arranged by size: 

                 683     616     450
 
BsrDI GCAATG_nn'

Cuts at:      0    1676    1749 
   Size:       1676      73
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      46     265    1749 
   Size:         46     219    1484

  Fragments arranged by size: 

                1484     219      46
 
BstBI TT'CG_AA

Cuts at:      0     708    1749 
   Size:        708    1041
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1214    1749 
   Size:       1214     535
 
BtsI GCAGTG_nn'

Cuts at:      0      33     431    1749 
   Size:         33     398    1318

  Fragments arranged by size: 

                1318     398      33
 
ClaI AT'CG_AT

Cuts at:      0     635    1749 
   Size:        635    1114
 
DraI TTT'AAA

Cuts at:      0     195    1749 
   Size:        195    1554
 
DraIII CAC_nnn'GTG

Cuts at:      0     931    1749 
   Size:        931     818
 
EarI CTCTTCn'nnn_

Cuts at:      0    1214    1609    1749 
   Size:       1214     395     140

  Fragments arranged by size: 

                1214     395     140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     929     974    1749 
   Size:        929      45     775

  Fragments arranged by size: 

                 929     775      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     911    1749 
   Size:        911     838
 
EcoO109I rG'GnC_Cy

Cuts at:      0     904    1749 
   Size:        904     845
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     126    1749 
   Size:        126    1623
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     667     699    1749 
   Size:         76      32     559      32    1050

  Fragments arranged by size: 

                1050     559      76      32      32
 
HincII GTy'rAC

Cuts at:      0     464    1749 
   Size:        464    1285
 
HindIII A'AGCT_T

Cuts at:      0     108     300     835    1749 
   Size:        108     192     535     914

  Fragments arranged by size: 

                 914     535     192     108
 
MslI CAynn'nnrTG

Cuts at:      0    1691    1749 
   Size:       1691      58
 
MspA1I CmG'CkG

Cuts at:      0     952    1749 
   Size:        952     797
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     655     931    1029    1749 
   Size:        655     276      98     720

  Fragments arranged by size: 

                 720     655     276      98
 
PfoI T'CCnGG_A

Cuts at:      0    1362    1749 
   Size:       1362     387
 
PpuMI rG'GwC_Cy

Cuts at:      0     904    1749 
   Size:        904     845
 
PsiI TTA'TAA

Cuts at:      0    1037    1749 
   Size:       1037     712
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     116     148    1240    1272    1749 
   Size:        116      32    1092      32     477

  Fragments arranged by size: 

                1092     477     116      32      32
 
PstI C_TGCA'G

Cuts at:      0    1332    1749 
   Size:       1332     417
 
PvuII CAG'CTG

Cuts at:      0     952    1749 
   Size:        952     797
 
SfcI C'TryA_G

Cuts at:      0     439    1328    1427    1749 
   Size:        439     889      99     322

  Fragments arranged by size: 

                 889     439     322      99
 
SmlI C'TyrA_G

Cuts at:      0      78     136    1145    1749 
   Size:         78      58    1009     604

  Fragments arranged by size: 

                1009     604      78      58
 
SspI AAT'ATT

Cuts at:      0      36    1450    1749 
   Size:         36    1414     299

  Fragments arranged by size: 

                1414     299      36
 
TatI w'GTAC_w

Cuts at:      0    1350    1749 
   Size:       1350     399
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1553    1585    1749 
   Size:       1553      32     164

  Fragments arranged by size: 

                1553     164      32
 
XbaI T'CTAG_A

Cuts at:      0     666    1749 
   Size:        666    1083
 
XhoI C'TCGA_G

Cuts at:      0    1145    1749 
   Size:       1145     604
 
XmnI GAAnn'nnTTC

Cuts at:      0     714    1749 
   Size:        714    1035
 

 Enzymes that do cut:

     AclI     AcuI    AflII     AlfI    AlwNI     ApoI     AseI     AvaI
    BanII     BbsI Bme1580I     BplI     BsaI     BsgI     BsmI    BsmBI
 Bsp1286I    BsrDI   BstAPI    BstBI    BtgZI     BtsI     ClaI     DraI
   DraIII     EarI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII
  HindIII     MslI   MspA1I    PflMI     PfoI    PpuMI     PsiI     PsrI
     PstI    PvuII     SfcI     SmlI     SspI     TatI     TstI     XbaI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XcmI
     XmaI     ZraI