Restriction map for
pFN21AE4142
(Linear) (Six-base) MAPSORT of: KIEE4142.seq Check: 454 from: 1 to: 1704
KIEE4142.orf
With 182 enzymes: SgfI *
March 22, 2012 17:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 755 1569 1684 1704
Size: 755 814 115 20
Fragments arranged by size:
814 755 115 20
AclI AA'CG_TT
Cuts at: 0 941 1704
Size: 941 763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 831 1116 1503 1704
Size: 85 746 285 387 201
Fragments arranged by size:
746 387 285 201 85
AfeI AGC'GCT
Cuts at: 0 301 1704
Size: 301 1403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 512 1704
Size: 512 1192
AlwNI CAG_nnn'CTG
Cuts at: 0 747 1704
Size: 747 957
ApaI G_GGCC'C
Cuts at: 0 171 1057 1642 1704
Size: 171 886 585 62
Fragments arranged by size:
886 585 171 62
AvaI C'yCGr_G
Cuts at: 0 902 1284 1603 1704
Size: 902 382 319 101
Fragments arranged by size:
902 382 319 101
BanI G'GyrC_C
Cuts at: 0 581 1597 1704
Size: 581 1016 107
Fragments arranged by size:
1016 581 107
BanII G_rGCy'C
Cuts at: 0 171 383 679 909 1057 1642 1704
Size: 171 212 296 230 148 585 62
Fragments arranged by size:
585 296 230 212 171 148 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 875 1122 1704
Size: 875 247 582
Fragments arranged by size:
875 582 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 985 1019 1704
Size: 985 34 685
Fragments arranged by size:
985 685 34
BfrBI ATG'CAT
Cuts at: 0 448 1704
Size: 448 1256
BglI GCCn_nnn'nGGC
Cuts at: 0 175 763 970 1606 1646 1704
Size: 175 588 207 636 40 58
Fragments arranged by size:
636 588 207 175 58 40
BlpI GC'TnA_GC
Cuts at: 0 521 1704
Size: 521 1183
Bme1580I G_kGCm'C
Cuts at: 0 171 830 1057 1602 1642 1704
Size: 171 659 227 545 40 62
Fragments arranged by size:
659 545 227 171 62 40
BmgBI CAC'GTC
Cuts at: 0 1128 1367 1704
Size: 1128 239 337
Fragments arranged by size:
1128 337 239
BmrI ACTGGGnnnn_n'
Cuts at: 0 1213 1704
Size: 1213 491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 244 277 1164 1276 1615 1704
Size: 115 129 33 887 112 339 89
Fragments arranged by size:
887 339 129 115 112 89 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1704
Size: 673 1031
BsaI GGTCTCn'nnnn_
Cuts at: 0 556 1704
Size: 556 1148
BsaBI GATnn'nnATC
Cuts at: 0 79 1704
Size: 79 1625
BsaHI Gr'CG_yC
Cuts at: 0 311 1704
Size: 311 1393
BsaWI w'CCGG_w
Cuts at: 0 188 394 600 1038 1565 1704
Size: 188 206 206 438 527 139
Fragments arranged by size:
527 438 206 206 188 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1195 1225 1578 1608 1704
Size: 1195 30 353 30 96
Fragments arranged by size:
1195 353 96 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1173 1176 1207 1210 1266 1442 1462 1599
Size: 1173 3 31 3 56 176 20 137
Cuts at: 1599 1704
Size: 105
Fragments arranged by size:
1173 176 137 105 56 31 20 3
3
BseYI C'CCAG_C
Cuts at: 0 1316 1704
Size: 1316 388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 226 732 1003 1704
Size: 69 157 506 271 701
Fragments arranged by size:
701 506 271 157 69
BsiEI CG_ry'CG
Cuts at: 0 1472 1704
Size: 1472 232
BsiHKAI G_wGCw'C
Cuts at: 0 263 527 845 1704
Size: 263 264 318 859
Fragments arranged by size:
859 318 264 263
BsmI GAATG_Cn'
Cuts at: 0 780 1001 1704
Size: 780 221 703
Fragments arranged by size:
780 703 221
Bsp1286I G_dGCh'C
Cuts at: 0 171 263 383 527 679 830 845 909
Size: 171 92 120 144 152 151 15 64
Cuts at: 909 1057 1602 1642 1704
Size: 148 545 40 62
Fragments arranged by size:
545 171 152 151 148 144 120 92
64 62 40 15
BspEI T'CCGG_A
Cuts at: 0 394 600 1565 1704
Size: 394 206 965 139
Fragments arranged by size:
965 394 206 139
BspHI T'CATG_A
Cuts at: 0 1016 1704
Size: 1016 688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 534 755 1569 1684 1704
Size: 534 221 814 115 20
Fragments arranged by size:
814 534 221 115 20
BsrBI CCG'CTC
Cuts at: 0 1506 1704
Size: 1506 198
BsrFI r'CCGG_y
Cuts at: 0 12 565 641 1704
Size: 12 553 76 1063
Fragments arranged by size:
1063 553 76 12
BstYI r'GATC_y
Cuts at: 0 1431 1704
Size: 1431 273
BtgI C'CryG_G
Cuts at: 0 482 671 989 1472 1704
Size: 482 189 318 483 232
Fragments arranged by size:
483 482 318 232 189
EaeI y'GGCC_r
Cuts at: 0 698 1156 1469 1704
Size: 698 458 313 235
Fragments arranged by size:
698 458 313 235
EagI C'GGCC_G
Cuts at: 0 1469 1704
Size: 1469 235
EarI CTCTTCn'nnn_
Cuts at: 0 19 40 1704
Size: 19 21 1664
Fragments arranged by size:
1664 21 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 525 1439 1704
Size: 525 914 265
Fragments arranged by size:
914 525 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 115 244 277 831 1116 1164 1276
Size: 85 30 129 33 554 285 48 112
Cuts at: 1276 1503 1615 1704
Size: 227 112 89
Fragments arranged by size:
554 285 227 129 112 112 89 85
48 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 1633 1704
Size: 97 1536 71
Fragments arranged by size:
1536 97 71
EcoO109I rG'GnC_Cy
Cuts at: 0 167 325 1053 1054 1638 1639 1704
Size: 167 158 728 1 584 1 65
Fragments arranged by size:
728 584 167 158 65 1 1
FspI TGC'GCA
Cuts at: 0 1526 1704
Size: 1526 178
HaeII r_GCGC'y
Cuts at: 0 223 303 414 1305 1552 1704
Size: 223 80 111 891 247 152
Fragments arranged by size:
891 247 223 152 111 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1704
Size: 916 32 756
Fragments arranged by size:
916 756 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1181 1704
Size: 236 945 523
Fragments arranged by size:
945 523 236
MscI TGG'CCA
Cuts at: 0 700 1704
Size: 700 1004
MspA1I CmG'CkG
Cuts at: 0 183 231 484 716 1316 1542 1704
Size: 183 48 253 232 600 226 162
Fragments arranged by size:
600 253 232 226 183 162 48
NaeI GCC'GGC
Cuts at: 0 14 1704
Size: 14 1690
NcoI C'CATG_G
Cuts at: 0 989 1704
Size: 989 715
NgoMIV G'CCGG_C
Cuts at: 0 12 1704
Size: 12 1692
NsiI A_TGCA'T
Cuts at: 0 450 1704
Size: 450 1254
NspI r_CATG'y
Cuts at: 0 147 1704
Size: 147 1557
PasI CC'CwG_GG
Cuts at: 0 722 768 1704
Size: 722 46 936
Fragments arranged by size:
936 722 46
PfoI T'CCnGG_A
Cuts at: 0 1253 1631 1704
Size: 1253 378 73
Fragments arranged by size:
1253 378 73
PspOMI G'GGCC_C
Cuts at: 0 167 1053 1638 1704
Size: 167 886 585 66
Fragments arranged by size:
886 585 167 66
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 271 303 1704
Size: 271 32 1401
Fragments arranged by size:
1401 271 32
PstI C_TGCA'G
Cuts at: 0 1052 1704
Size: 1052 652
PvuII CAG'CTG
Cuts at: 0 183 1316 1542 1704
Size: 183 1133 226 162
Fragments arranged by size:
1133 226 183 162
SacII CC_GC'GG
Cuts at: 0 485 1704
Size: 485 1219
SapI GCTCTTCn'nnn_
Cuts at: 0 19 1704
Size: 19 1685
SbfI CC_TGCA'GG
Cuts at: 0 1052 1704
Size: 1052 652
SfcI C'TryA_G
Cuts at: 0 1048 1704
Size: 1048 656
SmaI CCC'GGG
Cuts at: 0 904 1286 1704
Size: 904 382 418
Fragments arranged by size:
904 418 382
SmlI C'TyrA_G
Cuts at: 0 688 1704
Size: 688 1016
SrfI GCCC'GGGC
Cuts at: 0 1286 1704
Size: 1286 418
StyI C'CwwG_G
Cuts at: 0 347 695 989 1704
Size: 347 348 294 715
Fragments arranged by size:
715 348 347 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 1704
Size: 771 933
TatI w'GTAC_w
Cuts at: 0 131 1704
Size: 131 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 1195 1222 1227 1254 1578 1610
Size: 517 32 646 27 5 27 324 32
Cuts at: 1610 1704
Size: 94
Fragments arranged by size:
646 517 324 94 32 32 27 27
5
Tth111I GACn'n_nGTC
Cuts at: 0 271 1486 1704
Size: 271 1215 218
Fragments arranged by size:
1215 271 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1092 1639 1704
Size: 219 873 547 65
Fragments arranged by size:
873 547 219 65
XmaI C'CCGG_G
Cuts at: 0 902 1284 1704
Size: 902 382 420
Fragments arranged by size:
902 420 382
Enzymes that do cut:
AarI AclI AcuI AfeI AlfI AlwNI ApaI AvaI
BanI BanII BbsI BcgI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BstYI BtgI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PfoI PspOMI PsrI PstI PvuII SacII
SapI SbfI SfcI SmaI SmlI SrfI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BclI BglII BmtI BplI
Bpu10I BsaAI BsiWI BsmBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI NarI NdeI NheI NotI NruI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI XbaI XhoI XmnI ZraI