(Linear) (Six-base) MAPSORT of: KIEE4141.seq  Check: 8605  from: 1  to: 1674



KIEE4141.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     113    1555    1674 
   Size:        103      10    1442     119

  Fragments arranged by size: 

                1442     119     103      10
 
AflIII A'CryG_T

Cuts at:      0     946    1674 
   Size:        946     728
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     449    1674 
   Size:        449    1225
 
ApoI r'AATT_y

Cuts at:      0     217     439     479     520    1674 
   Size:        217     222      40      41    1154

  Fragments arranged by size: 

                1154     222     217      41      40
 
BamHI G'GATC_C

Cuts at:      0     876    1674 
   Size:        876     798
 
BanI G'GyrC_C

Cuts at:      0    1504    1674 
   Size:       1504     170
 
BanII G_rGCy'C

Cuts at:      0    1143    1674 
   Size:       1143     531
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     632     666    1674 
   Size:        632      34    1008

  Fragments arranged by size: 

                1008     632      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1537    1674 
   Size:       1537     137
 
BfrBI ATG'CAT

Cuts at:      0      73     283     789    1674 
   Size:         73     210     506     885

  Fragments arranged by size: 

                 885     506     210      73
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1274    1674 
   Size:       1274     400
 
BglII A'GATC_T

Cuts at:      0    1596    1674 
   Size:       1596      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     983    1625    1674 
   Size:        983     642      49

  Fragments arranged by size: 

                 983     642      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1127    1335    1559    1674 
   Size:       1127     208     224     115

  Fragments arranged by size: 

                1127     224     208     115
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1674 
   Size:        352    1322
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1649    1674 
   Size:       1649      25
 
BsaBI GATnn'nnATC

Cuts at:      0     689    1595    1674 
   Size:        689     906      79

  Fragments arranged by size: 

                 906     689      79
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1109    1275    1674 
   Size:       1109     166     399

  Fragments arranged by size: 

                1109     399     166
 
BsiHKAI G_wGCw'C

Cuts at:      0    1381    1674 
   Size:       1381     293
 
BsmI GAATG_Cn'

Cuts at:      0      73     428     655    1441    1674 
   Size:         73     355     227     786     233

  Fragments arranged by size: 

                 786     355     233     227      73
 
Bsp1286I G_dGCh'C

Cuts at:      0    1143    1381    1674 
   Size:       1143     238     293

  Fragments arranged by size: 

                1143     293     238
 
BspHI T'CATG_A

Cuts at:      0     627    1514    1674 
   Size:        627     887     160

  Fragments arranged by size: 

                 887     627     160
 
BsrBI CCG'CTC

Cuts at:      0     381     758    1674 
   Size:        381     377     916

  Fragments arranged by size: 

                 916     381     377
 
BsrDI GCAATG_nn'

Cuts at:      0     197    1674 
   Size:        197    1477
 
BstYI r'GATC_y

Cuts at:      0     342     690     752     876    1019    1596    1674 
   Size:        342     348      62     124     143     577      78

  Fragments arranged by size: 

                 577     348     342     143     124      78      62
 
Bsu36I CC'TnA_GG

Cuts at:      0     714    1674 
   Size:        714     960
 
DraI TTT'AAA

Cuts at:      0     222    1175    1674 
   Size:        222     953     499

  Fragments arranged by size: 

                 953     499     222
 
DraIII CAC_nnn'GTG

Cuts at:      0    1016    1674 
   Size:       1016     658
 
EaeI y'GGCC_r

Cuts at:      0    1266    1674 
   Size:       1266     408
 
EarI CTCTTCn'nnn_

Cuts at:      0     122    1145    1674 
   Size:        122    1023     529

  Fragments arranged by size: 

                1023     529     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     113    1127    1335    1555    1559    1674 
   Size:        103      10    1014     208     220       4     115

  Fragments arranged by size: 

                1014     220     208     115     103      10       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     331    1674 
   Size:        331    1343
 
EcoO109I rG'GnC_Cy

Cuts at:      0     955    1674 
   Size:        955     719
 
EcoRI G'AATT_C

Cuts at:      0     479    1674 
   Size:        479    1195
 
EcoRV GAT'ATC

Cuts at:      0    1326    1674 
   Size:       1326     348
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     567    1674 
   Size:        567    1107
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486    1674 
   Size:        454      32    1188

  Fragments arranged by size: 

                1188     454      32
 
MscI TGG'CCA

Cuts at:      0    1268    1674 
   Size:       1268     406
 
MslI CAynn'nnrTG

Cuts at:      0    1652    1674 
   Size:       1652      22
 
MspA1I CmG'CkG

Cuts at:      0     202     340    1674 
   Size:        202     138    1334

  Fragments arranged by size: 

                1334     202     138
 
NdeI CA'TA_TG

Cuts at:      0     871    1674 
   Size:        871     803
 
NsiI A_TGCA'T

Cuts at:      0      75     285     791    1674 
   Size:         75     210     506     883

  Fragments arranged by size: 

                 883     506     210      75
 
NspI r_CATG'y

Cuts at:      0     950    1674 
   Size:        950     724
 
PciI A'CATG_T

Cuts at:      0     946    1674 
   Size:        946     728
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1369    1674 
   Size:       1369     305
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1144    1176    1674 
   Size:       1144      32     498

  Fragments arranged by size: 

                1144     498      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     955    1674 
   Size:        955     719
 
PvuII CAG'CTG

Cuts at:      0     202    1674 
   Size:        202    1472
 
SfcI C'TryA_G

Cuts at:      0     349     981    1278    1674 
   Size:        349     632     297     396

  Fragments arranged by size: 

                 632     396     349     297
 
SmlI C'TyrA_G

Cuts at:      0     331    1674 
   Size:        331    1343
 
StuI AGG'CCT

Cuts at:      0     269     719    1674 
   Size:        269     450     955

  Fragments arranged by size: 

                 955     450     269
 
StyI C'CwwG_G

Cuts at:      0    1097    1674 
   Size:       1097     577
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1090    1640    1674 
   Size:       1090     550      34

  Fragments arranged by size: 

                1090     550      34
 
TatI w'GTAC_w

Cuts at:      0     698    1674 
   Size:        698     976
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1338    1370    1674 
   Size:       1338      32     304

  Fragments arranged by size: 

                1338     304      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     614    1674 
   Size:        614    1060
 
XmnI GAAnn'nnTTC

Cuts at:      0     521     545    1674 
   Size:        521      24    1129

  Fragments arranged by size: 

                1129     521      24
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI    BamHI     BanI    BanII     BcgI
    BciVI    BfrBI     BglI    BglII     BmrI     BpmI    BpuEI     BsaI
    BsaBI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrBI    BsrDI
    BstYI   Bsu36I     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I     MscI     MslI   MspA1I
     NdeI     NsiI     NspI     PciI    PflMI     PpiI    PpuMI    PvuII
     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI     BclI
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI
     EagI     EciI  EcoICRI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     XmaI     ZraI