Restriction map for
pFN21AE4139
(Linear) (Six-base) MAPSORT of: KIEE4139.seq Check: 9479 from: 1 to: 1665
KIEE4139.orf
With 182 enzymes: SgfI *
March 22, 2012 17:27 ..
AccI GT'mk_AC
Cuts at: 0 1173 1665
Size: 1173 492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1635 1665
Size: 589 1046 30
Fragments arranged by size:
1046 589 30
AleI CACnn'nnGTG
Cuts at: 0 293 1665
Size: 293 1372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 1009 1380 1665
Size: 947 62 371 285
Fragments arranged by size:
947 371 285 62
AlwNI CAG_nnn'CTG
Cuts at: 0 102 720 1665
Size: 102 618 945
Fragments arranged by size:
945 618 102
ApaLI G'TGCA_C
Cuts at: 0 229 1365 1665
Size: 229 1136 300
Fragments arranged by size:
1136 300 229
ApoI r'AATT_y
Cuts at: 0 376 424 710 1665
Size: 376 48 286 955
Fragments arranged by size:
955 376 286 48
AvaI C'yCGr_G
Cuts at: 0 50 65 1665
Size: 50 15 1600
Fragments arranged by size:
1600 50 15
BanI G'GyrC_C
Cuts at: 0 252 1665
Size: 252 1413
BanII G_rGCy'C
Cuts at: 0 45 1447 1665
Size: 45 1402 218
Fragments arranged by size:
1402 218 45
BbeI G_GCGC'C
Cuts at: 0 256 1665
Size: 256 1409
BbsI GAAGACnn'nnnn_
Cuts at: 0 1429 1599 1664 1665
Size: 1429 170 65 1
Fragments arranged by size:
1429 170 65 1
BbvCI CC'TCA_GC
Cuts at: 0 673 1665
Size: 673 992
BciVI GTATCCnnnnn_n'
Cuts at: 0 1560 1665
Size: 1560 105
BglI GCCn_nnn'nGGC
Cuts at: 0 70 261 1665
Size: 70 191 1404
Fragments arranged by size:
1404 191 70
BglII A'GATC_T
Cuts at: 0 1314 1665
Size: 1314 351
Bme1580I G_kGCm'C
Cuts at: 0 233 1369 1413 1665
Size: 233 1136 44 252
Fragments arranged by size:
1136 252 233 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 1163 1665
Size: 1163 502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 925 1665
Size: 512 413 740
Fragments arranged by size:
740 512 413
Bpu10I CC'TnA_GC
Cuts at: 0 673 1665
Size: 673 992
BsaBI GATnn'nnATC
Cuts at: 0 1520 1665
Size: 1520 145
BsaHI Gr'CG_yC
Cuts at: 0 253 1665
Size: 253 1412
BsaWI w'CCGG_w
Cuts at: 0 587 1665
Size: 587 1078
BseYI C'CCAG_C
Cuts at: 0 214 326 1665
Size: 214 112 1339
Fragments arranged by size:
1339 214 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1665
Size: 907 758
BsiEI CG_ry'CG
Cuts at: 0 34 145 261 1665
Size: 34 111 116 1404
Fragments arranged by size:
1404 116 111 34
BsiHKAI G_wGCw'C
Cuts at: 0 45 233 845 1102 1369 1665
Size: 45 188 612 257 267 296
Fragments arranged by size:
612 296 267 257 188 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 354 1665
Size: 354 1311
Bsp1286I G_dGCh'C
Cuts at: 0 45 233 845 1102 1369 1413 1447 1665
Size: 45 188 612 257 267 44 34 218
Fragments arranged by size:
612 267 257 218 188 45 44 34
BspEI T'CCGG_A
Cuts at: 0 587 1665
Size: 587 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1536 1665
Size: 1536 129
BsrBI CCG'CTC
Cuts at: 0 449 1665
Size: 449 1216
BsrDI GCAATG_nn'
Cuts at: 0 1421 1427 1665
Size: 1421 6 238
Fragments arranged by size:
1421 238 6
BsrFI r'CCGG_y
Cuts at: 0 151 158 249 260 1665
Size: 151 7 91 11 1405
Fragments arranged by size:
1405 151 91 11 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 1665
Size: 102 1563
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 1665
Size: 837 828
BstYI r'GATC_y
Cuts at: 0 1314 1338 1521 1665
Size: 1314 24 183 144
Fragments arranged by size:
1314 183 144 24
BstZ17I GTA'TAC
Cuts at: 0 1174 1665
Size: 1174 491
BtgI C'CryG_G
Cuts at: 0 356 836 1665
Size: 356 480 829
Fragments arranged by size:
829 480 356
DraIII CAC_nnn'GTG
Cuts at: 0 1406 1603 1665
Size: 1406 197 62
Fragments arranged by size:
1406 197 62
EaeI y'GGCC_r
Cuts at: 0 142 1505 1665
Size: 142 1363 160
Fragments arranged by size:
1363 160 142
EagI C'GGCC_G
Cuts at: 0 142 1665
Size: 142 1523
EarI CTCTTCn'nnn_
Cuts at: 0 310 1019 1385 1665
Size: 310 709 366 280
Fragments arranged by size:
709 366 310 280
EcoICRI GAG'CTC
Cuts at: 0 43 1665
Size: 43 1622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 589 925 1635 1665
Size: 512 77 336 710 30
Fragments arranged by size:
710 512 336 77 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 676 1665
Size: 676 989
EcoO109I rG'GnC_Cy
Cuts at: 0 510 1665
Size: 510 1155
EcoRI G'AATT_C
Cuts at: 0 376 424 1665
Size: 376 48 1241
Fragments arranged by size:
1241 376 48
EcoRV GAT'ATC
Cuts at: 0 1134 1665
Size: 1134 531
FspI TGC'GCA
Cuts at: 0 388 547 1665
Size: 388 159 1118
Fragments arranged by size:
1118 388 159
HaeII r_GCGC'y
Cuts at: 0 203 256 1665
Size: 203 53 1409
Fragments arranged by size:
1409 203 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1225 1257 1534 1566 1579 1611 1665
Size: 1225 32 277 32 13 32 54
Fragments arranged by size:
1225 277 54 32 32 32 13
HincII GTy'rAC
Cuts at: 0 1567 1665
Size: 1567 98
HindIII A'AGCT_T
Cuts at: 0 1647 1665
Size: 1647 18
KasI G'GCGC_C
Cuts at: 0 252 1665
Size: 252 1413
MslI CAynn'nnrTG
Cuts at: 0 293 1362 1665
Size: 293 1069 303
Fragments arranged by size:
1069 303 293
MspA1I CmG'CkG
Cuts at: 0 102 358 958 1665
Size: 102 256 600 707
Fragments arranged by size:
707 600 256 102
NaeI GCC'GGC
Cuts at: 0 153 160 251 1665
Size: 153 7 91 1414
Fragments arranged by size:
1414 153 91 7
NarI GG'CG_CC
Cuts at: 0 253 1665
Size: 253 1412
NcoI C'CATG_G
Cuts at: 0 836 1665
Size: 836 829
NgoMIV G'CCGG_C
Cuts at: 0 151 158 249 1665
Size: 151 7 91 1416
Fragments arranged by size:
1416 151 91 7
NspI r_CATG'y
Cuts at: 0 1062 1579 1665
Size: 1062 517 86
Fragments arranged by size:
1062 517 86
PasI CC'CwG_GG
Cuts at: 0 460 1554 1665
Size: 460 1094 111
Fragments arranged by size:
1094 460 111
PstI C_TGCA'G
Cuts at: 0 98 518 897 1665
Size: 98 420 379 768
Fragments arranged by size:
768 420 379 98
PvuII CAG'CTG
Cuts at: 0 102 958 1665
Size: 102 856 707
Fragments arranged by size:
856 707 102
SacI G_AGCT'C
Cuts at: 0 45 1665
Size: 45 1620
SacII CC_GC'GG
Cuts at: 0 359 1665
Size: 359 1306
SfcI C'TryA_G
Cuts at: 0 94 514 893 1665
Size: 94 420 379 772
Fragments arranged by size:
772 420 379 94
SfoI GGC'GCC
Cuts at: 0 254 1665
Size: 254 1411
SmaI CCC'GGG
Cuts at: 0 67 1665
Size: 67 1598
SphI G_CATG'C
Cuts at: 0 1579 1665
Size: 1579 86
SspI AAT'ATT
Cuts at: 0 867 1665
Size: 867 798
StyI C'CwwG_G
Cuts at: 0 836 935 1494 1570 1665
Size: 836 99 559 76 95
Fragments arranged by size:
836 559 99 95 76
XmaI C'CCGG_G
Cuts at: 0 65 1665
Size: 65 1600
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BciVI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI BstZ17I BtgI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII KasI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PstI
PvuII SacI SacII SfcI SfoI SmaI SphI SspI
StyI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BclI BfrBI BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaXI BseRI BsiWI BsmI BspHI
BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EciI FalI FseI FspAI
HpaI KpnI MfeI MluI MmeI MscI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI