Restriction map for
pFN21AE4135
(Linear) (Six-base) MAPSORT of: KIEE4135.seq Check: 1565 from: 1 to: 1629
KIEE4135.orf
With 182 enzymes: SgfI *
March 22, 2012 17:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 502 1403 1550 1629
Size: 328 174 901 147 79
Fragments arranged by size:
901 328 174 147 79
AflIII A'CryG_T
Cuts at: 0 443 1629
Size: 443 1186
AleI CACnn'nnGTG
Cuts at: 0 1042 1629
Size: 1042 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1108 1629
Size: 1108 521
AlwNI CAG_nnn'CTG
Cuts at: 0 369 1186 1629
Size: 369 817 443
Fragments arranged by size:
817 443 369
ApaLI G'TGCA_C
Cuts at: 0 787 1629
Size: 787 842
ApoI r'AATT_y
Cuts at: 0 9 425 619 1629
Size: 9 416 194 1010
Fragments arranged by size:
1010 416 194 9
AvaI C'yCGr_G
Cuts at: 0 1019 1054 1629
Size: 1019 35 575
Fragments arranged by size:
1019 575 35
BanI G'GyrC_C
Cuts at: 0 1192 1629
Size: 1192 437
BanII G_rGCy'C
Cuts at: 0 203 560 1459 1629
Size: 203 357 899 170
Fragments arranged by size:
899 357 203 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 1500 1629
Size: 1500 129
BbvCI CC'TCA_GC
Cuts at: 0 410 1629
Size: 410 1219
BglII A'GATC_T
Cuts at: 0 1362 1629
Size: 1362 267
Bme1580I G_kGCm'C
Cuts at: 0 791 1055 1629
Size: 791 264 574
Fragments arranged by size:
791 574 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 1005 1087 1629
Size: 1005 82 542
Fragments arranged by size:
1005 542 82
BmtI G_CTAG'C
Cuts at: 0 74 1629
Size: 74 1555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 1629
Size: 492 1137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1015 1473 1629
Size: 116 899 458 156
Fragments arranged by size:
899 458 156 116
Bpu10I CC'TnA_GC
Cuts at: 0 410 554 1629
Size: 410 144 1075
Fragments arranged by size:
1075 410 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 152 1629
Size: 9 143 1477
Fragments arranged by size:
1477 143 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 208 211 427 1246 1629
Size: 103 105 3 216 819 383
Fragments arranged by size:
819 383 216 105 103 3
BseYI C'CCAG_C
Cuts at: 0 599 1629
Size: 599 1030
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1629
Size: 872 757
BsiHKAI G_wGCw'C
Cuts at: 0 203 560 791 1011 1459 1629
Size: 203 357 231 220 448 170
Fragments arranged by size:
448 357 231 220 203 170
BsmI GAATG_Cn'
Cuts at: 0 478 1629
Size: 478 1151
Bsp1286I G_dGCh'C
Cuts at: 0 203 560 791 1011 1055 1459 1629
Size: 203 357 231 220 44 404 170
Fragments arranged by size:
404 357 231 220 203 170 44
BspHI T'CATG_A
Cuts at: 0 176 1629
Size: 176 1453
BsrFI r'CCGG_y
Cuts at: 0 1025 1629
Size: 1025 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1472 1629
Size: 225 1247 157
Fragments arranged by size:
1247 225 157
BstYI r'GATC_y
Cuts at: 0 1362 1629
Size: 1362 267
Bsu36I CC'TnA_GG
Cuts at: 0 15 1445 1629
Size: 15 1430 184
Fragments arranged by size:
1430 184 15
BtgI C'CryG_G
Cuts at: 0 104 387 958 1629
Size: 104 283 571 671
Fragments arranged by size:
671 571 283 104
BtsI GCAGTG_nn'
Cuts at: 0 905 1090 1629
Size: 905 185 539
Fragments arranged by size:
905 539 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 906 941 1629
Size: 906 35 688
Fragments arranged by size:
906 688 35
DraIII CAC_nnn'GTG
Cuts at: 0 1081 1629
Size: 1081 548
EaeI y'GGCC_r
Cuts at: 0 879 1629
Size: 879 750
EcoICRI GAG'CTC
Cuts at: 0 201 558 1457 1629
Size: 201 357 899 172
Fragments arranged by size:
899 357 201 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 328 502 1015 1403 1473 1550 1629
Size: 116 212 174 513 388 70 77 79
Fragments arranged by size:
513 388 212 174 116 79 77 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 1444 1629
Size: 310 1134 185
Fragments arranged by size:
1134 310 185
EcoRI G'AATT_C
Cuts at: 0 9 1629
Size: 9 1620
EcoRV GAT'ATC
Cuts at: 0 654 1629
Size: 654 975
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 755 1629
Size: 755 874
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 938 970 1090 1122 1629
Size: 643 32 263 32 120 32 507
Fragments arranged by size:
643 507 263 120 32 32 32
HincII GTy'rAC
Cuts at: 0 993 1629
Size: 993 636
HindIII A'AGCT_T
Cuts at: 0 762 1549 1629
Size: 762 787 80
Fragments arranged by size:
787 762 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1133 1629
Size: 326 807 496
Fragments arranged by size:
807 496 326
MscI TGG'CCA
Cuts at: 0 881 1629
Size: 881 748
MslI CAynn'nnrTG
Cuts at: 0 223 1042 1629
Size: 223 819 587
Fragments arranged by size:
819 587 223
MspA1I CmG'CkG
Cuts at: 0 50 453 603 1351 1487 1629
Size: 50 403 150 748 136 142
Fragments arranged by size:
748 403 150 142 136 50
NcoI C'CATG_G
Cuts at: 0 104 387 1629
Size: 104 283 1242
Fragments arranged by size:
1242 283 104
NheI G'CTAG_C
Cuts at: 0 70 1629
Size: 70 1559
NspI r_CATG'y
Cuts at: 0 447 969 1344 1629
Size: 447 522 375 285
Fragments arranged by size:
522 447 375 285
PciI A'CATG_T
Cuts at: 0 443 1629
Size: 443 1186
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 369 831 1629
Size: 255 114 462 798
Fragments arranged by size:
798 462 255 114
PfoI T'CCnGG_A
Cuts at: 0 1511 1629
Size: 1511 118
PstI C_TGCA'G
Cuts at: 0 341 608 1629
Size: 341 267 1021
Fragments arranged by size:
1021 341 267
PvuII CAG'CTG
Cuts at: 0 50 453 603 1351 1487 1629
Size: 50 403 150 748 136 142
Fragments arranged by size:
748 403 150 142 136 50
SacI G_AGCT'C
Cuts at: 0 203 560 1459 1629
Size: 203 357 899 170
Fragments arranged by size:
899 357 203 170
SfcI C'TryA_G
Cuts at: 0 337 604 1629
Size: 337 267 1025
Fragments arranged by size:
1025 337 267
SmaI CCC'GGG
Cuts at: 0 1056 1629
Size: 1056 573
SmlI C'TyrA_G
Cuts at: 0 24 167 1629
Size: 24 143 1462
Fragments arranged by size:
1462 143 24
SphI G_CATG'C
Cuts at: 0 1344 1629
Size: 1344 285
StuI AGG'CCT
Cuts at: 0 1450 1629
Size: 1450 179
StyI C'CwwG_G
Cuts at: 0 104 387 1629
Size: 104 283 1242
Fragments arranged by size:
1242 283 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 1069 1532 1629
Size: 134 935 463 97
Fragments arranged by size:
935 463 134 97
XbaI T'CTAG_A
Cuts at: 0 189 1629
Size: 189 1440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 965 1074 1629
Size: 591 374 109 555
Fragments arranged by size:
591 555 374 109
XmaI C'CCGG_G
Cuts at: 0 1054 1629
Size: 1054 575
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrFI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NcoI NheI NspI PciI PflMI PfoI PstI
PvuII SacI SfcI SmaI SmlI SphI StuI StyI
TaqII XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI
DrdI EagI EarI EciI EcoO109I FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XhoI XmnI ZraI