Restriction map for
pFN21AE4134
(Linear) (Six-base) MAPSORT of: KIEE4134.seq Check: 2717 from: 1 to: 1617
KIEE4134.orf
With 182 enzymes: SgfI *
January 19, 2012 13:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 623 1617
Size: 623 994
AatII G_ACGT'C
Cuts at: 0 128 1617
Size: 128 1489
AccI GT'mk_AC
Cuts at: 0 1103 1617
Size: 1103 514
Acc65I G'GTAC_C
Cuts at: 0 971 1617
Size: 971 646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 553 1617
Size: 262 291 1064
Fragments arranged by size:
1064 291 262
AhdI GACnn_n'nnGTC
Cuts at: 0 957 1617
Size: 957 660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 308 318 764 1617
Size: 308 10 446 853
Fragments arranged by size:
853 446 308 10
AlwNI CAG_nnn'CTG
Cuts at: 0 594 1617
Size: 594 1023
ApoI r'AATT_y
Cuts at: 0 485 853 895 1617
Size: 485 368 42 722
Fragments arranged by size:
722 485 368 42
AvaI C'yCGr_G
Cuts at: 0 278 380 827 1426 1617
Size: 278 102 447 599 191
Fragments arranged by size:
599 447 278 191 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 444 477 1617
Size: 444 33 1140
Fragments arranged by size:
1140 444 33
BanI G'GyrC_C
Cuts at: 0 971 1492 1559 1617
Size: 971 521 67 58
Fragments arranged by size:
971 521 67 58
BanII G_rGCy'C
Cuts at: 0 650 758 1617
Size: 650 108 859
Fragments arranged by size:
859 650 108
BbeI G_GCGC'C
Cuts at: 0 1563 1617
Size: 1563 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 1617
Size: 93 1524
BciVI GTATCCnnnnn_n'
Cuts at: 0 800 1617
Size: 800 817
BglI GCCn_nnn'nGGC
Cuts at: 0 703 1617
Size: 703 914
BlpI GC'TnA_GC
Cuts at: 0 1433 1617
Size: 1433 184
Bme1580I G_kGCm'C
Cuts at: 0 1495 1617
Size: 1495 122
BmgBI CAC'GTC
Cuts at: 0 8 99 1617
Size: 8 91 1518
Fragments arranged by size:
1518 91 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1116 1236 1617
Size: 1116 120 381
Fragments arranged by size:
1116 381 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 658 745 1617
Size: 526 132 87 872
Fragments arranged by size:
872 526 132 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1567 1617
Size: 1567 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 1343 1617
Size: 1343 274
BsaHI Gr'CG_yC
Cuts at: 0 125 683 1560 1617
Size: 125 558 877 57
Fragments arranged by size:
877 558 125 57
BsaWI w'CCGG_w
Cuts at: 0 847 1617
Size: 847 770
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1120 1150 1617
Size: 1120 30 467
Fragments arranged by size:
1120 467 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 859 1177 1195 1198 1201 1210 1291
Size: 49 810 318 18 3 3 9 81
Cuts at: 1291 1294 1303 1386 1389 1617
Size: 3 9 83 3 228
Fragments arranged by size:
810 318 228 83 81 49 18 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 425 1043 1071 1443 1617
Size: 425 618 28 372 174
Fragments arranged by size:
618 425 372 174 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 310 1021 1617
Size: 99 211 711 596
Fragments arranged by size:
711 596 211 99
BsiEI CG_ry'CG
Cuts at: 0 404 1617
Size: 404 1213
BsiHKAI G_wGCw'C
Cuts at: 0 389 650 758 1617
Size: 389 261 108 859
Fragments arranged by size:
859 389 261 108
Bsp1286I G_dGCh'C
Cuts at: 0 389 650 758 1495 1617
Size: 389 261 108 737 122
Fragments arranged by size:
737 389 261 122 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 74 623 983 1043 1617
Size: 52 22 549 360 60 574
Fragments arranged by size:
574 549 360 60 52 22
BsrBI CCG'CTC
Cuts at: 0 237 603 1617
Size: 237 366 1014
Fragments arranged by size:
1014 366 237
BsrDI GCAATG_nn'
Cuts at: 0 821 1617
Size: 821 796
BsrFI r'CCGG_y
Cuts at: 0 1201 1617
Size: 1201 416
BsrGI T'GTAC_A
Cuts at: 0 1019 1617
Size: 1019 598
BssSI C'ACGA_G
Cuts at: 0 768 1617
Size: 768 849
BstEII G'GTnAC_C
Cuts at: 0 39 273 1617
Size: 39 234 1344
Fragments arranged by size:
1344 234 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 1617
Size: 222 1395
Bsu36I CC'TnA_GG
Cuts at: 0 466 595 889 1617
Size: 466 129 294 728
Fragments arranged by size:
728 466 294 129
BtgI C'CryG_G
Cuts at: 0 793 1617
Size: 793 824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1274 1455 1617
Size: 1274 181 162
Fragments arranged by size:
1274 181 162
BtsI GCAGTG_nn'
Cuts at: 0 1447 1541 1617
Size: 1447 94 76
Fragments arranged by size:
1447 94 76
EaeI y'GGCC_r
Cuts at: 0 2 93 212 401 524 962 1363 1588
Size: 2 91 119 189 123 438 401 225
Cuts at: 1588 1617
Size: 29
Fragments arranged by size:
438 401 225 189 123 119 91 29
2
EagI C'GGCC_G
Cuts at: 0 401 1617
Size: 401 1216
EarI CTCTTCn'nnn_
Cuts at: 0 26 1145 1163 1172 1187 1259 1268 1280
Size: 26 1119 18 9 15 72 9 12
Cuts at: 1280 1333 1617
Size: 53 284
Fragments arranged by size:
1119 284 72 53 26 18 15 12
9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 1617
Size: 118 1499
EcoICRI GAG'CTC
Cuts at: 0 648 756 1617
Size: 648 108 861
Fragments arranged by size:
861 648 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 526 553 658 745 1617
Size: 262 264 27 105 87 872
Fragments arranged by size:
872 264 262 105 87 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1617
Size: 31 1586
EcoO109I rG'GnC_Cy
Cuts at: 0 72 392 1617
Size: 72 320 1225
Fragments arranged by size:
1225 320 72
EcoRI G'AATT_C
Cuts at: 0 853 895 1617
Size: 853 42 722
Fragments arranged by size:
853 722 42
EcoRV GAT'ATC
Cuts at: 0 519 1617
Size: 519 1098
HaeII r_GCGC'y
Cuts at: 0 1387 1563 1617
Size: 1387 176 54
Fragments arranged by size:
1387 176 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 1120 1152 1234 1266 1454 1486
Size: 943 32 145 32 82 32 188 32
Cuts at: 1486 1617
Size: 131
Fragments arranged by size:
943 188 145 131 82 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1559 1617
Size: 1559 58
KpnI G_GTAC'C
Cuts at: 0 975 1617
Size: 975 642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1617
Size: 219 1398
MscI TGG'CCA
Cuts at: 0 4 95 214 1590 1617
Size: 4 91 119 1376 27
Fragments arranged by size:
1376 119 91 27 4
MspA1I CmG'CkG
Cuts at: 0 113 627 748 981 1617
Size: 113 514 121 233 636
Fragments arranged by size:
636 514 233 121 113
NaeI GCC'GGC
Cuts at: 0 1203 1617
Size: 1203 414
NarI GG'CG_CC
Cuts at: 0 1560 1617
Size: 1560 57
NgoMIV G'CCGG_C
Cuts at: 0 1201 1617
Size: 1201 416
PflMI CCAn_nnn'nTGG
Cuts at: 0 1372 1617
Size: 1372 245
PfoI T'CCnGG_A
Cuts at: 0 542 1617
Size: 542 1075
PpuMI rG'GwC_Cy
Cuts at: 0 72 1617
Size: 72 1545
PspXI vC'TCGA_Gb
Cuts at: 0 827 1617
Size: 827 790
PstI C_TGCA'G
Cuts at: 0 753 980 1617
Size: 753 227 637
Fragments arranged by size:
753 637 227
PvuII CAG'CTG
Cuts at: 0 113 627 748 981 1617
Size: 113 514 121 233 636
Fragments arranged by size:
636 514 233 121 113
SacI G_AGCT'C
Cuts at: 0 650 758 1617
Size: 650 108 859
Fragments arranged by size:
859 650 108
SanDI GG'GwC_CC
Cuts at: 0 72 1617
Size: 72 1545
SfcI C'TryA_G
Cuts at: 0 749 976 1617
Size: 749 227 641
Fragments arranged by size:
749 641 227
SfoI GGC'GCC
Cuts at: 0 1561 1617
Size: 1561 56
SmaI CCC'GGG
Cuts at: 0 280 382 1617
Size: 280 102 1235
Fragments arranged by size:
1235 280 102
SmlI C'TyrA_G
Cuts at: 0 827 1582 1617
Size: 827 755 35
Fragments arranged by size:
827 755 35
StuI AGG'CCT
Cuts at: 0 887 1617
Size: 887 730
TatI w'GTAC_w
Cuts at: 0 557 1019 1617
Size: 557 462 598
Fragments arranged by size:
598 557 462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1363 1395 1617
Size: 982 32 349 32 222
Fragments arranged by size:
982 349 222 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 828 1617
Size: 828 789
XhoI C'TCGA_G
Cuts at: 0 827 1617
Size: 827 790
XmaI C'CCGG_G
Cuts at: 0 278 380 1617
Size: 278 102 1237
Fragments arranged by size:
1237 278 102
XmnI GAAnn'nnTTC
Cuts at: 0 802 1617
Size: 802 815
ZraI GAC'GTC
Cuts at: 0 126 1617
Size: 126 1491
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AlfI AlwNI
ApoI AvaI BaeI BanI BanII BbeI BbsI BciVI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII
BstXI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I KasI KpnI MmeI MscI MspA1I NaeI NarI
NgoMIV PflMI PfoI PpuMI PspXI PstI PvuII SacI
SanDI SfcI SfoI SmaI SmlI StuI TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BamHI BbvCI BcgI
BclI BfrBI BglII BmtI BplI Bpu10I BsaAI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BssHII BstAPI BstBI
BstYI BstZ17I ClaI CspCI DraI DraIII DrdI FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII Tth111I XbaI