Restriction map for
pFN21AE4132
(Linear) (Six-base) MAPSORT of: KIEE4132.seq Check: 8373 from: 1 to: 1599
KIEE4132.orf
With 182 enzymes: SgfI *
March 22, 2012 17:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 311 1065 1258 1599
Size: 311 754 193 341
Fragments arranged by size:
754 341 311 193
Acc65I G'GTAC_C
Cuts at: 0 1215 1599
Size: 1215 384
AhdI GACnn_n'nnGTC
Cuts at: 0 79 1599
Size: 79 1520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 812 1599
Size: 776 36 787
Fragments arranged by size:
787 776 36
AlwNI CAG_nnn'CTG
Cuts at: 0 69 1057 1599
Size: 69 988 542
Fragments arranged by size:
988 542 69
ApaI G_GGCC'C
Cuts at: 0 705 1599
Size: 705 894
AvaI C'yCGr_G
Cuts at: 0 442 686 704 1220 1408 1508 1599
Size: 442 244 18 516 188 100 91
Fragments arranged by size:
516 442 244 188 100 91 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 23 56 1599
Size: 23 33 1543
Fragments arranged by size:
1543 33 23
BanI G'GyrC_C
Cuts at: 0 1215 1344 1599
Size: 1215 129 255
Fragments arranged by size:
1215 255 129
BanII G_rGCy'C
Cuts at: 0 166 365 383 656 705 890 1037 1207
Size: 166 199 18 273 49 185 147 170
Cuts at: 1207 1599
Size: 392
Fragments arranged by size:
392 273 199 185 170 166 147 49
18
BbsI GAAGACnn'nnnn_
Cuts at: 0 993 1599
Size: 993 606
BbvCI CC'TCA_GC
Cuts at: 0 208 1599
Size: 208 1391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1466 1500 1599
Size: 1466 34 99
Fragments arranged by size:
1466 99 34
BglI GCCn_nnn'nGGC
Cuts at: 0 355 412 1599
Size: 355 57 1187
Fragments arranged by size:
1187 355 57
BglII A'GATC_T
Cuts at: 0 558 1599
Size: 558 1041
BlpI GC'TnA_GC
Cuts at: 0 359 1297 1599
Size: 359 938 302
Fragments arranged by size:
938 359 302
Bme1580I G_kGCm'C
Cuts at: 0 34 630 705 1599
Size: 34 596 75 894
Fragments arranged by size:
894 596 75 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 884 912 1599
Size: 884 28 687
Fragments arranged by size:
884 687 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 1065 1599
Size: 291 774 534
Fragments arranged by size:
774 534 291
Bpu10I CC'TnA_GC
Cuts at: 0 7 208 1031 1599
Size: 7 201 823 568
Fragments arranged by size:
823 568 201 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1599
Size: 1201 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 1012 1599
Size: 439 573 587
Fragments arranged by size:
587 573 439
BsaAI yAC'GTr
Cuts at: 0 1116 1599
Size: 1116 483
BsaWI w'CCGG_w
Cuts at: 0 97 1599
Size: 97 1502
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 1093 1123 1599
Size: 838 30 225 30 476
Fragments arranged by size:
838 476 225 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 283 409 922 1599
Size: 67 216 126 513 677
Fragments arranged by size:
677 513 216 126 67
BseYI C'CCAG_C
Cuts at: 0 1275 1594 1599
Size: 1275 319 5
Fragments arranged by size:
1275 319 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1043 1570 1599
Size: 401 642 527 29
Fragments arranged by size:
642 527 401 29
BsiHKAI G_wGCw'C
Cuts at: 0 166 365 383 1037 1409 1599
Size: 166 199 18 654 372 190
Fragments arranged by size:
654 372 199 190 166 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 87 600 1599
Size: 87 513 999
Fragments arranged by size:
999 513 87
Bsp1286I G_dGCh'C
Cuts at: 0 34 166 365 383 630 656 705 890
Size: 34 132 199 18 247 26 49 185
Cuts at: 890 1037 1207 1409 1599
Size: 147 170 202 190
Fragments arranged by size:
247 202 199 190 185 170 147 132
49 34 26 18
BspEI T'CCGG_A
Cuts at: 0 97 1599
Size: 97 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 311 1065 1258 1301 1599
Size: 77 234 754 193 43 298
Fragments arranged by size:
754 298 234 193 77 43
BsrBI CCG'CTC
Cuts at: 0 11 1121 1599
Size: 11 1110 478
Fragments arranged by size:
1110 478 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 864 1599
Size: 864 735
BstYI r'GATC_y
Cuts at: 0 558 1599
Size: 558 1041
BtgI C'CryG_G
Cuts at: 0 239 1599
Size: 239 1360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 492 1599
Size: 492 1107
BtsI GCAGTG_nn'
Cuts at: 0 698 1537 1599
Size: 698 839 62
Fragments arranged by size:
839 698 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 453 1599
Size: 453 1146
EaeI y'GGCC_r
Cuts at: 0 118 492 1140 1227 1599
Size: 118 374 648 87 372
Fragments arranged by size:
648 374 372 118 87
EarI CTCTTCn'nnn_
Cuts at: 0 171 1599
Size: 171 1428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 1599
Size: 259 1340
EcoICRI GAG'CTC
Cuts at: 0 164 363 381 1035 1599
Size: 164 199 18 654 564
Fragments arranged by size:
654 564 199 164 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 1065 1599
Size: 291 774 534
Fragments arranged by size:
774 534 291
EcoO109I rG'GnC_Cy
Cuts at: 0 66 204 468 1290 1599
Size: 66 138 264 822 309
Fragments arranged by size:
822 309 264 138 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 543 575 1599
Size: 65 32 446 32 1024
Fragments arranged by size:
1024 446 65 32 32
HindIII A'AGCT_T
Cuts at: 0 1299 1599
Size: 1299 300
KpnI G_GTAC'C
Cuts at: 0 1219 1599
Size: 1219 380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 881 1599
Size: 239 642 718
Fragments arranged by size:
718 642 239
MscI TGG'CCA
Cuts at: 0 120 1142 1229 1599
Size: 120 1022 87 370
Fragments arranged by size:
1022 370 120 87
MslI CAynn'nnrTG
Cuts at: 0 995 1599
Size: 995 604
MspA1I CmG'CkG
Cuts at: 0 346 573 716 756 1090 1599
Size: 346 227 143 40 334 509
Fragments arranged by size:
509 346 334 227 143 40
NcoI C'CATG_G
Cuts at: 0 239 1599
Size: 239 1360
NspI r_CATG'y
Cuts at: 0 501 1599
Size: 501 1098
PasI CC'CwG_GG
Cuts at: 0 27 253 978 1599
Size: 27 226 725 621
Fragments arranged by size:
725 621 226 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 1149 1236 1599
Size: 576 573 87 363
Fragments arranged by size:
576 573 363 87
PfoI T'CCnGG_A
Cuts at: 0 365 1599
Size: 365 1234
PpuMI rG'GwC_Cy
Cuts at: 0 66 1290 1599
Size: 66 1224 309
Fragments arranged by size:
1224 309 66
PspOMI G'GGCC_C
Cuts at: 0 701 1599
Size: 701 898
PstI C_TGCA'G
Cuts at: 0 155 1391 1599
Size: 155 1236 208
Fragments arranged by size:
1236 208 155
PvuII CAG'CTG
Cuts at: 0 346 573 756 1090 1599
Size: 346 227 183 334 509
Fragments arranged by size:
509 346 334 227 183
SacI G_AGCT'C
Cuts at: 0 166 365 383 1037 1599
Size: 166 199 18 654 562
Fragments arranged by size:
654 562 199 166 18
SapI GCTCTTCn'nnn_
Cuts at: 0 171 1599
Size: 171 1428
SfcI C'TryA_G
Cuts at: 0 151 311 846 1387 1416 1599
Size: 151 160 535 541 29 183
Fragments arranged by size:
541 535 183 160 151 29
SmaI CCC'GGG
Cuts at: 0 706 1599
Size: 706 893
SmlI C'TyrA_G
Cuts at: 0 1180 1599
Size: 1180 419
SrfI GCCC'GGGC
Cuts at: 0 706 1599
Size: 706 893
StuI AGG'CCT
Cuts at: 0 406 1309 1506 1599
Size: 406 903 197 93
Fragments arranged by size:
903 406 197 93
StyI C'CwwG_G
Cuts at: 0 239 464 947 1501 1599
Size: 239 225 483 554 98
Fragments arranged by size:
554 483 239 225 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 313 970 1237 1267 1599
Size: 76 237 657 267 30 332
Fragments arranged by size:
657 332 267 237 76 30
XmaI C'CCGG_G
Cuts at: 0 704 1599
Size: 704 895
Enzymes that do cut:
AarI Acc65I AhdI AlfI AlwNI ApaI AvaI BaeI
BanI BanII BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SfcI SmaI SmlI SrfI StuI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BciVI BclI BfrBI BmgBI BmtI BplI
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI