Restriction map for
pFN21AE4128
(Linear) (Six-base) MAPSORT of: KIEE4128.seq Check: 565 from: 1 to: 1578
KIEE4128.orf
With 182 enzymes: SgfI *
March 22, 2012 17:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 374 1014 1578
Size: 374 640 564
Fragments arranged by size:
640 564 374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 1578
Size: 648 930
AhdI GACnn_n'nnGTC
Cuts at: 0 930 1578
Size: 930 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1153 1578
Size: 1036 117 425
Fragments arranged by size:
1036 425 117
ApaI G_GGCC'C
Cuts at: 0 115 1578
Size: 115 1463
ApoI r'AATT_y
Cuts at: 0 301 1578
Size: 301 1277
AscI GG'CGCG_CC
Cuts at: 0 1066 1578
Size: 1066 512
AvaI C'yCGr_G
Cuts at: 0 108 213 321 636 727 823 1499 1578
Size: 108 105 108 315 91 96 676 79
Fragments arranged by size:
676 315 108 108 105 96 91 79
AvrII C'CTAG_G
Cuts at: 0 69 426 1326 1578
Size: 69 357 900 252
Fragments arranged by size:
900 357 252 69
BamHI G'GATC_C
Cuts at: 0 125 1578
Size: 125 1453
BanI G'GyrC_C
Cuts at: 0 266 317 879 1281 1394 1578
Size: 266 51 562 402 113 184
Fragments arranged by size:
562 402 266 184 113 51
BanII G_rGCy'C
Cuts at: 0 115 1578
Size: 115 1463
BbeI G_GCGC'C
Cuts at: 0 270 883 1398 1578
Size: 270 613 515 180
Fragments arranged by size:
613 515 270 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 384 1578
Size: 384 1194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 981 1015 1578
Size: 981 34 563
Fragments arranged by size:
981 563 34
BglI GCCn_nnn'nGGC
Cuts at: 0 745 760 791 1010 1578
Size: 745 15 31 219 568
Fragments arranged by size:
745 568 219 31 15
BglII A'GATC_T
Cuts at: 0 548 1530 1578
Size: 548 982 48
Fragments arranged by size:
982 548 48
BlpI GC'TnA_GC
Cuts at: 0 187 1456 1578
Size: 187 1269 122
Fragments arranged by size:
1269 187 122
Bme1580I G_kGCm'C
Cuts at: 0 115 220 541 1246 1578
Size: 115 105 321 705 332
Fragments arranged by size:
705 332 321 115 105
BmgBI CAC'GTC
Cuts at: 0 558 1206 1578
Size: 558 648 372
Fragments arranged by size:
648 558 372
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 878 985 1544 1578
Size: 429 449 107 559 34
Fragments arranged by size:
559 449 429 107 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1120 1578
Size: 1120 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1429 1510 1578
Size: 490 939 81 68
Fragments arranged by size:
939 490 81 68
Bpu10I CC'TnA_GC
Cuts at: 0 334 1578
Size: 334 1244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1090 1578
Size: 1042 48 488
Fragments arranged by size:
1042 488 48
BsaAI yAC'GTr
Cuts at: 0 372 1578
Size: 372 1206
BsaHI Gr'CG_yC
Cuts at: 0 247 267 819 880 1395 1578
Size: 247 20 552 61 515 183
Fragments arranged by size:
552 515 247 183 61 20
BsaWI w'CCGG_w
Cuts at: 0 41 1578
Size: 41 1537
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 1363 1393 1507 1537 1578
Size: 535 30 798 30 114 30 41
Fragments arranged by size:
798 535 114 41 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 268 1213 1578
Size: 93 175 945 365
Fragments arranged by size:
945 365 175 93
BseYI C'CCAG_C
Cuts at: 0 225 485 1092 1224 1578
Size: 225 260 607 132 354
Fragments arranged by size:
607 354 260 225 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 352 580 1042 1578
Size: 328 24 228 462 536
Fragments arranged by size:
536 462 328 228 24
BsiEI CG_ry'CG
Cuts at: 0 315 755 1578
Size: 315 440 823
Fragments arranged by size:
823 440 315
BsiHKAI G_wGCw'C
Cuts at: 0 85 377 692 1578
Size: 85 292 315 886
Fragments arranged by size:
886 315 292 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 678 1578
Size: 678 900
Bsp1286I G_dGCh'C
Cuts at: 0 85 115 220 377 541 692 1246 1578
Size: 85 30 105 157 164 151 554 332
Fragments arranged by size:
554 332 164 157 151 105 85 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 1014 1578
Size: 374 640 564
Fragments arranged by size:
640 564 374
BsrBI CCG'CTC
Cuts at: 0 912 1578
Size: 912 666
BsrFI r'CCGG_y
Cuts at: 0 314 1391 1578
Size: 314 1077 187
Fragments arranged by size:
1077 314 187
BssHII G'CGCG_C
Cuts at: 0 191 193 195 205 295 458 460 574
Size: 191 2 2 10 90 163 2 114
Cuts at: 574 589 1066 1578
Size: 15 477 512
Fragments arranged by size:
512 477 191 163 114 90 15 10
2 2 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1232 1578
Size: 684 548 346
Fragments arranged by size:
684 548 346
BstYI r'GATC_y
Cuts at: 0 125 548 1530 1578
Size: 125 423 982 48
Fragments arranged by size:
982 423 125 48
Bsu36I CC'TnA_GG
Cuts at: 0 1314 1578
Size: 1314 264
BtgI C'CryG_G
Cuts at: 0 242 626 758 773 1262 1578
Size: 242 384 132 15 489 316
Fragments arranged by size:
489 384 316 242 132 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 684 822 1578
Size: 684 138 756
Fragments arranged by size:
756 684 138
EaeI y'GGCC_r
Cuts at: 0 360 402 597 752 770 1002 1578
Size: 360 42 195 155 18 232 576
Fragments arranged by size:
576 360 232 195 155 42 18
EagI C'GGCC_G
Cuts at: 0 752 1578
Size: 752 826
EarI CTCTTCn'nnn_
Cuts at: 0 667 1578
Size: 667 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1021 1578
Size: 1021 557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 648 1429 1510 1578
Size: 490 158 781 81 68
Fragments arranged by size:
781 490 158 81 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1012 1578
Size: 1012 566
EcoO109I rG'GnC_Cy
Cuts at: 0 112 423 1545 1578
Size: 112 311 1122 33
Fragments arranged by size:
1122 311 112 33
EcoRI G'AATT_C
Cuts at: 0 301 1578
Size: 301 1277
FspI TGC'GCA
Cuts at: 0 1042 1423 1578
Size: 1042 381 155
Fragments arranged by size:
1042 381 155
FspAI rTGC'GCAy
Cuts at: 0 1042 1578
Size: 1042 536
HaeII r_GCGC'y
Cuts at: 0 154 270 883 1398 1578
Size: 154 116 613 515 180
Fragments arranged by size:
613 515 180 154 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 847 879 916 948 1459 1491
Size: 535 32 280 32 37 32 511 32
Cuts at: 1491 1507 1539 1578
Size: 16 32 39
Fragments arranged by size:
535 511 280 39 37 32 32 32
32 32 16
KasI G'GCGC_C
Cuts at: 0 266 879 1394 1578
Size: 266 613 515 184
Fragments arranged by size:
613 515 266 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 578 1457 1578
Size: 230 348 879 121
Fragments arranged by size:
879 348 230 121
MscI TGG'CCA
Cuts at: 0 362 404 1578
Size: 362 42 1174
Fragments arranged by size:
1174 362 42
MslI CAynn'nnrTG
Cuts at: 0 682 1339 1578
Size: 682 657 239
Fragments arranged by size:
682 657 239
MspA1I CmG'CkG
Cuts at: 0 131 186 244 668 775 1189 1578
Size: 131 55 58 424 107 414 389
Fragments arranged by size:
424 414 389 131 107 58 55
NarI GG'CG_CC
Cuts at: 0 267 880 1395 1578
Size: 267 613 515 183
Fragments arranged by size:
613 515 267 183
NcoI C'CATG_G
Cuts at: 0 626 1578
Size: 626 952
NotI GC'GGCC_GC
Cuts at: 0 752 1578
Size: 752 826
PmlI CAC'GTG
Cuts at: 0 372 1578
Size: 372 1206
PspOMI G'GGCC_C
Cuts at: 0 111 1578
Size: 111 1467
PspXI vC'TCGA_Gb
Cuts at: 0 823 1578
Size: 823 755
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 625 657 1578
Size: 625 32 921
Fragments arranged by size:
921 625 32
PstI C_TGCA'G
Cuts at: 0 239 1530 1578
Size: 239 1291 48
Fragments arranged by size:
1291 239 48
PvuII CAG'CTG
Cuts at: 0 186 668 1189 1578
Size: 186 482 521 389
Fragments arranged by size:
521 482 389 186
SacII CC_GC'GG
Cuts at: 0 245 776 1578
Size: 245 531 802
Fragments arranged by size:
802 531 245
SapI GCTCTTCn'nnn_
Cuts at: 0 667 1578
Size: 667 911
SbfI CC_TGCA'GG
Cuts at: 0 239 1578
Size: 239 1339
ScaI AGT'ACT
Cuts at: 0 829 1578
Size: 829 749
SfcI C'TryA_G
Cuts at: 0 235 1526 1578
Size: 235 1291 52
Fragments arranged by size:
1291 235 52
SfoI GGC'GCC
Cuts at: 0 268 881 1396 1578
Size: 268 613 515 182
Fragments arranged by size:
613 515 268 182
SmaI CCC'GGG
Cuts at: 0 110 215 323 638 1578
Size: 110 105 108 315 940
Fragments arranged by size:
940 315 110 108 105
SmlI C'TyrA_G
Cuts at: 0 823 1057 1105 1578
Size: 823 234 48 473
Fragments arranged by size:
823 473 234 48
StyI C'CwwG_G
Cuts at: 0 69 426 626 1326 1578
Size: 69 357 200 700 252
Fragments arranged by size:
700 357 252 200 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 1144 1578
Size: 157 987 434
Fragments arranged by size:
987 434 157
TatI w'GTAC_w
Cuts at: 0 827 1578
Size: 827 751
XbaI T'CTAG_A
Cuts at: 0 1533 1578
Size: 1533 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1232 1578
Size: 1232 346
XhoI C'TCGA_G
Cuts at: 0 823 1578
Size: 823 755
XmaI C'CCGG_G
Cuts at: 0 108 213 321 636 1578
Size: 108 105 108 315 942
Fragments arranged by size:
942 315 108 108 105
Enzymes that do cut:
AarI AcuI AhdI AlfI ApaI ApoI AscI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
NotI PmlI PspOMI PspXI PsrI PstI PvuII SacII
SapI SbfI ScaI SfcI SfoI SmaI SmlI StyI
TaqII TatI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaLI AseI AsiSI BaeI BbvCI
BciVI BclI BfrBI BmtI BsaI BsaBI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PvuI RsrII SacI SalI SanDI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XmnI ZraI