Restriction map for
pFN21AE4127
(Linear) (Six-base) MAPSORT of: KIEE4127.seq Check: 4209 from: 1 to: 1560
KIEE4127.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
Acc65I G'GTAC_C
Cuts at: 0 1100 1560
Size: 1100 460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 397 1560
Size: 97 300 1163
Fragments arranged by size:
1163 300 97
AfeI AGC'GCT
Cuts at: 0 75 1560
Size: 75 1485
AgeI A'CCGG_T
Cuts at: 0 504 1560
Size: 504 1056
AleI CACnn'nnGTG
Cuts at: 0 1204 1560
Size: 1204 356
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1560
Size: 511 32 1017
Fragments arranged by size:
1017 511 32
ApaI G_GGCC'C
Cuts at: 0 721 1265 1560
Size: 721 544 295
Fragments arranged by size:
721 544 295
ApaLI G'TGCA_C
Cuts at: 0 967 1560
Size: 967 593
AvaI C'yCGr_G
Cuts at: 0 886 1266 1303 1320 1332 1560
Size: 886 380 37 17 12 228
Fragments arranged by size:
886 380 228 37 17 12
BanI G'GyrC_C
Cuts at: 0 202 258 631 687 814 1100 1325 1560
Size: 202 56 373 56 127 286 225 235
Fragments arranged by size:
373 286 235 225 202 127 56 56
BanII G_rGCy'C
Cuts at: 0 158 721 925 1265 1387 1560
Size: 158 563 204 340 122 173
Fragments arranged by size:
563 340 204 173 158 122
BbeI G_GCGC'C
Cuts at: 0 206 262 635 1329 1560
Size: 206 56 373 694 231
Fragments arranged by size:
694 373 231 206 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 1560
Size: 104 1456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 867 901 1414 1448 1560
Size: 867 34 513 34 112
Fragments arranged by size:
867 513 112 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 169 277 380 475 1391 1560
Size: 169 108 103 95 916 169
Fragments arranged by size:
916 169 169 108 103 95
Bme1580I G_kGCm'C
Cuts at: 0 416 692 721 819 971 1265 1448 1560
Size: 416 276 29 98 152 294 183 112
Fragments arranged by size:
416 294 276 183 152 112 98 29
BmgBI CAC'GTC
Cuts at: 0 555 1560
Size: 555 1005
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 615 1360 1560
Size: 615 745 200
Fragments arranged by size:
745 615 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1560
Size: 1330 230
Bpu10I CC'TnA_GC
Cuts at: 0 77 1560
Size: 77 1483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 1560
Size: 492 1068
BsaAI yAC'GTr
Cuts at: 0 429 1560
Size: 429 1131
BsaHI Gr'CG_yC
Cuts at: 0 203 259 298 335 632 956 1326 1560
Size: 203 56 39 37 297 324 370 234
Fragments arranged by size:
370 324 297 234 203 56 39 37
BsaWI w'CCGG_w
Cuts at: 0 504 1560
Size: 504 1056
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 1560
Size: 122 30 1408
Fragments arranged by size:
1408 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 859 1206 1215 1218 1221 1224 1227 1560
Size: 859 347 9 3 3 3 3 333
Fragments arranged by size:
859 347 333 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 302 1258 1560
Size: 302 956 302
Fragments arranged by size:
956 302 302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 1560
Size: 1499 61
BsiEI CG_ry'CG
Cuts at: 0 221 269 1560
Size: 221 48 1291
Fragments arranged by size:
1291 221 48
BsiHKAI G_wGCw'C
Cuts at: 0 428 596 971 1211 1560
Size: 428 168 375 240 349
Fragments arranged by size:
428 375 349 240 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 847 1560
Size: 847 713
Bsp1286I G_dGCh'C
Cuts at: 0 158 416 428 596 692 721 819 925
Size: 158 258 12 168 96 29 98 106
Cuts at: 925 971 1211 1265 1387 1448 1560
Size: 46 240 54 122 61 112
Fragments arranged by size:
258 240 168 158 122 112 106 98
96 61 54 46 29 12
BsrBI CCG'CTC
Cuts at: 0 1280 1344 1560
Size: 1280 64 216
Fragments arranged by size:
1280 216 64
BsrFI r'CCGG_y
Cuts at: 0 216 234 504 1560
Size: 216 18 270 1056
Fragments arranged by size:
1056 270 216 18
BssHII G'CGCG_C
Cuts at: 0 105 838 1560
Size: 105 733 722
Fragments arranged by size:
733 722 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1412 1560
Size: 1412 148
BstEII G'GTnAC_C
Cuts at: 0 669 1560
Size: 669 891
BstYI r'GATC_y
Cuts at: 0 881 1560
Size: 881 679
BtgI C'CryG_G
Cuts at: 0 167 254 395 1295 1560
Size: 167 87 141 900 265
Fragments arranged by size:
900 265 167 141 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 846 1560
Size: 846 714
ClaI AT'CG_AT
Cuts at: 0 938 1560
Size: 938 622
DraIII CAC_nnn'GTG
Cuts at: 0 401 1560
Size: 401 1159
EaeI y'GGCC_r
Cuts at: 0 218 266 431 557 663 1151 1310 1560
Size: 218 48 165 126 106 488 159 250
Fragments arranged by size:
488 250 218 165 159 126 106 48
EagI C'GGCC_G
Cuts at: 0 218 266 1560
Size: 218 48 1294
Fragments arranged by size:
1294 218 48
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1245 1560
Size: 1216 29 315
Fragments arranged by size:
1216 315 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1233 1560
Size: 1233 327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 397 1330 1560
Size: 97 300 933 230
Fragments arranged by size:
933 300 230 97
EcoO109I rG'GnC_Cy
Cuts at: 0 718 1056 1262 1300 1560
Size: 718 338 206 38 260
Fragments arranged by size:
718 338 260 206 38
FspI TGC'GCA
Cuts at: 0 871 1560
Size: 871 689
FspAI rTGC'GCAy
Cuts at: 0 871 1560
Size: 871 689
HaeII r_GCGC'y
Cuts at: 0 77 206 262 635 1329 1560
Size: 77 129 56 373 694 231
Fragments arranged by size:
694 373 231 129 77 56
KasI G'GCGC_C
Cuts at: 0 202 258 631 1325 1560
Size: 202 56 373 694 235
Fragments arranged by size:
694 373 235 202 56
KpnI G_GTAC'C
Cuts at: 0 1104 1560
Size: 1104 456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1560
Size: 977 583
MscI TGG'CCA
Cuts at: 0 559 1560
Size: 559 1001
MslI CAynn'nnrTG
Cuts at: 0 679 865 1204 1560
Size: 679 186 339 356
Fragments arranged by size:
679 356 339 186
MspA1I CmG'CkG
Cuts at: 0 117 256 589 1170 1297 1560
Size: 117 139 333 581 127 263
Fragments arranged by size:
581 333 263 139 127 117
NaeI GCC'GGC
Cuts at: 0 218 236 1560
Size: 218 18 1324
Fragments arranged by size:
1324 218 18
NarI GG'CG_CC
Cuts at: 0 203 259 632 1326 1560
Size: 203 56 373 694 234
Fragments arranged by size:
694 373 234 203 56
NgoMIV G'CCGG_C
Cuts at: 0 216 234 1560
Size: 216 18 1326
Fragments arranged by size:
1326 216 18
PmlI CAC'GTG
Cuts at: 0 429 1560
Size: 429 1131
PpuMI rG'GwC_Cy
Cuts at: 0 1300 1560
Size: 1300 260
PspOMI G'GGCC_C
Cuts at: 0 717 1261 1560
Size: 717 544 299
Fragments arranged by size:
717 544 299
PspXI vC'TCGA_Gb
Cuts at: 0 886 1560
Size: 886 674
PvuII CAG'CTG
Cuts at: 0 117 1560
Size: 117 1443
SacII CC_GC'GG
Cuts at: 0 257 1298 1560
Size: 257 1041 262
Fragments arranged by size:
1041 262 257
SanDI GG'GwC_CC
Cuts at: 0 1300 1560
Size: 1300 260
SapI GCTCTTCn'nnn_
Cuts at: 0 1216 1560
Size: 1216 344
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 475 1560
Size: 475 1085
SfoI GGC'GCC
Cuts at: 0 204 260 633 1327 1560
Size: 204 56 373 694 233
Fragments arranged by size:
694 373 233 204 56
SgrAI Cr'CCGG_yG
Cuts at: 0 504 1560
Size: 504 1056
SmaI CCC'GGG
Cuts at: 0 1268 1334 1560
Size: 1268 66 226
Fragments arranged by size:
1268 226 66
SmlI C'TyrA_G
Cuts at: 0 471 886 1560
Size: 471 415 674
Fragments arranged by size:
674 471 415
SspI AAT'ATT
Cuts at: 0 1553 1560
Size: 1553 7
StuI AGG'CCT
Cuts at: 0 469 1560
Size: 469 1091
StyI C'CwwG_G
Cuts at: 0 1247 1560
Size: 1247 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 98 1057 1560
Size: 98 959 503
Fragments arranged by size:
959 503 98
TatI w'GTAC_w
Cuts at: 0 890 1139 1560
Size: 890 249 421
Fragments arranged by size:
890 421 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1495 1527 1560
Size: 1495 32 33
Fragments arranged by size:
1495 33 32
XhoI C'TCGA_G
Cuts at: 0 886 1560
Size: 886 674
XmaI C'CCGG_G
Cuts at: 0 1266 1332 1560
Size: 1266 66 228
Fragments arranged by size:
1266 228 66
Enzymes that do cut:
Acc65I AcuI AfeI AgeI AleI AloI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrBI BsrFI BssHII BstAPI BstEII BstYI BtgI BtgZI
ClaI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
FspI FspAI HaeII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PmlI PpuMI PspOMI PspXI
PvuII SacII SanDI SapI SfiI SfoI SgrAI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AhdI AlfI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BsaI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BssSI BstBI BstXI BstZ17I Bsu36I BtsI CspCI
DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
Hin4I HincII HindIII HpaI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI
PvuI RsrII SacI SalI SbfI ScaI SexAI SfcI
SgfI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI XmnI ZraI