Restriction map for
pFN21AE4125
(Linear) (Six-base) MAPSORT of: KIEE4125.seq Check: 2293 from: 1 to: 1539
KIEE4125.orf
With 182 enzymes: SgfI *
March 22, 2012 17:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 340 1539
Size: 340 1199
AatII G_ACGT'C
Cuts at: 0 329 1539
Size: 329 1210
AflIII A'CryG_T
Cuts at: 0 308 1539
Size: 308 1231
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1095 1539
Size: 135 960 444
Fragments arranged by size:
960 444 135
ApaI G_GGCC'C
Cuts at: 0 595 1539
Size: 595 944
AscI GG'CGCG_CC
Cuts at: 0 1069 1539
Size: 1069 470
AvaI C'yCGr_G
Cuts at: 0 172 360 663 1216 1305 1539
Size: 172 188 303 553 89 234
Fragments arranged by size:
553 303 234 188 172 89
BanI G'GyrC_C
Cuts at: 0 867 958 1539
Size: 867 91 581
Fragments arranged by size:
867 581 91
BanII G_rGCy'C
Cuts at: 0 155 595 898 1103 1136 1409 1539
Size: 155 440 303 205 33 273 130
Fragments arranged by size:
440 303 273 205 155 130 33
BbeI G_GCGC'C
Cuts at: 0 871 962 1539
Size: 871 91 577
Fragments arranged by size:
871 577 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 1539
Size: 795 744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 273 307 666 700 1539
Size: 273 34 359 34 839
Fragments arranged by size:
839 359 273 34 34
BlpI GC'TnA_GC
Cuts at: 0 46 1474 1539
Size: 46 1428 65
Fragments arranged by size:
1428 65 46
Bme1580I G_kGCm'C
Cuts at: 0 595 1539
Size: 595 944
BmrI ACTGGGnnnn_n'
Cuts at: 0 468 1357 1539
Size: 468 889 182
Fragments arranged by size:
889 468 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1423 1539
Size: 862 561 116
Fragments arranged by size:
862 561 116
Bpu10I CC'TnA_GC
Cuts at: 0 1324 1336 1539
Size: 1324 12 203
Fragments arranged by size:
1324 203 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1393 1539
Size: 259 1134 146
Fragments arranged by size:
1134 259 146
BsaAI yAC'GTr
Cuts at: 0 1056 1539
Size: 1056 483
BsaBI GATnn'nnATC
Cuts at: 0 196 1539
Size: 196 1343
BsaHI Gr'CG_yC
Cuts at: 0 62 326 868 959 1539
Size: 62 264 542 91 580
Fragments arranged by size:
580 542 264 91 62
BsaWI w'CCGG_w
Cuts at: 0 1436 1539
Size: 1436 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 805 835 1372 1402 1539
Size: 439 30 336 30 537 30 137
Fragments arranged by size:
537 439 336 137 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 535 775 778 781 1506 1539
Size: 460 75 240 3 3 725 33
Fragments arranged by size:
725 460 240 75 33 3 3
BseYI C'CCAG_C
Cuts at: 0 748 871 1095 1539
Size: 748 123 224 444
Fragments arranged by size:
748 444 224 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 267 1101 1539
Size: 36 231 834 438
Fragments arranged by size:
834 438 231 36
BsiEI CG_ry'CG
Cuts at: 0 142 354 599 1539
Size: 142 212 245 940
Fragments arranged by size:
940 245 212 142
BsiHKAI G_wGCw'C
Cuts at: 0 704 1303 1409 1539
Size: 704 599 106 130
Fragments arranged by size:
704 599 130 106
Bsp1286I G_dGCh'C
Cuts at: 0 155 595 704 898 1103 1136 1303 1409
Size: 155 440 109 194 205 33 167 106
Cuts at: 1409 1539
Size: 130
Fragments arranged by size:
440 205 194 167 155 130 109 106
33
BspEI T'CCGG_A
Cuts at: 0 1436 1539
Size: 1436 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 515 1539
Size: 340 175 1024
Fragments arranged by size:
1024 340 175
BsrBI CCG'CTC
Cuts at: 0 543 1340 1394 1539
Size: 543 797 54 145
Fragments arranged by size:
797 543 145 54
BssHII G'CGCG_C
Cuts at: 0 41 393 428 1069 1539
Size: 41 352 35 641 470
Fragments arranged by size:
641 470 352 41 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 1539
Size: 135 1404
Bsu36I CC'TnA_GG
Cuts at: 0 754 1539
Size: 754 785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 329 450 1146 1539
Size: 329 121 696 393
Fragments arranged by size:
696 393 329 121
DrdI GACnn_nn'nnGTC
Cuts at: 0 325 1539
Size: 325 1214
EaeI y'GGCC_r
Cuts at: 0 139 596 920 1157 1205 1539
Size: 139 457 324 237 48 334
Fragments arranged by size:
457 334 324 237 139 48
EagI C'GGCC_G
Cuts at: 0 139 596 1539
Size: 139 457 943
Fragments arranged by size:
943 457 139
EarI CTCTTCn'nnn_
Cuts at: 0 545 709 1150 1174 1539
Size: 545 164 441 24 365
Fragments arranged by size:
545 441 365 164 24
EcoICRI GAG'CTC
Cuts at: 0 1407 1539
Size: 1407 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1423 1539
Size: 862 561 116
Fragments arranged by size:
862 561 116
EcoNI CCTnn'n_nnAGG
Cuts at: 0 757 1006 1539
Size: 757 249 533
Fragments arranged by size:
757 533 249
EcoO109I rG'GnC_Cy
Cuts at: 0 365 591 1539
Size: 365 226 948
Fragments arranged by size:
948 365 226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1539
Size: 900 639
FspI TGC'GCA
Cuts at: 0 1045 1539
Size: 1045 494
HaeII r_GCGC'y
Cuts at: 0 96 501 624 871 962 1042 1539
Size: 96 405 123 247 91 80 497
Fragments arranged by size:
497 405 247 123 96 91 80
HincII GTy'rAC
Cuts at: 0 726 1539
Size: 726 813
KasI G'GCGC_C
Cuts at: 0 867 958 1539
Size: 867 91 581
Fragments arranged by size:
867 581 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1539
Size: 169 1370
MscI TGG'CCA
Cuts at: 0 922 1159 1539
Size: 922 237 380
Fragments arranged by size:
922 380 237
MslI CAynn'nnrTG
Cuts at: 0 1261 1539
Size: 1261 278
MspA1I CmG'CkG
Cuts at: 0 132 255 900 1115 1473 1539
Size: 132 123 645 215 358 66
Fragments arranged by size:
645 358 215 132 123 66
NarI GG'CG_CC
Cuts at: 0 868 959 1539
Size: 868 91 580
Fragments arranged by size:
868 580 91
NotI GC'GGCC_GC
Cuts at: 0 139 1539
Size: 139 1400
NspI r_CATG'y
Cuts at: 0 630 1539
Size: 630 909
PasI CC'CwG_GG
Cuts at: 0 1080 1539
Size: 1080 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 1080 1317 1539
Size: 1080 237 222
Fragments arranged by size:
1080 237 222
PfoI T'CCnGG_A
Cuts at: 0 440 1539
Size: 440 1099
PspOMI G'GGCC_C
Cuts at: 0 591 1539
Size: 591 948
PstI C_TGCA'G
Cuts at: 0 512 1091 1539
Size: 512 579 448
Fragments arranged by size:
579 512 448
PvuII CAG'CTG
Cuts at: 0 1473 1539
Size: 1473 66
SacI G_AGCT'C
Cuts at: 0 1409 1539
Size: 1409 130
SapI GCTCTTCn'nnn_
Cuts at: 0 709 1150 1539
Size: 709 441 389
Fragments arranged by size:
709 441 389
ScaI AGT'ACT
Cuts at: 0 220 1539
Size: 220 1319
SexAI A'CCwGG_T
Cuts at: 0 914 1539
Size: 914 625
SfcI C'TryA_G
Cuts at: 0 36 508 1087 1539
Size: 36 472 579 452
Fragments arranged by size:
579 472 452 36
SfoI GGC'GCC
Cuts at: 0 869 960 1539
Size: 869 91 579
Fragments arranged by size:
869 579 91
SmaI CCC'GGG
Cuts at: 0 362 1307 1539
Size: 362 945 232
Fragments arranged by size:
945 362 232
SmlI C'TyrA_G
Cuts at: 0 274 1408 1539
Size: 274 1134 131
Fragments arranged by size:
1134 274 131
SphI G_CATG'C
Cuts at: 0 630 1539
Size: 630 909
StuI AGG'CCT
Cuts at: 0 280 1539
Size: 280 1259
TatI w'GTAC_w
Cuts at: 0 218 455 1539
Size: 218 237 1084
Fragments arranged by size:
1084 237 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 805 837 1372 1404 1539
Size: 805 32 535 32 135
Fragments arranged by size:
805 535 135 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1288 1539
Size: 1288 251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1269 1539
Size: 1269 270
XmaI C'CCGG_G
Cuts at: 0 360 1305 1539
Size: 360 945 234
Fragments arranged by size:
945 360 234
ZraI GAC'GTC
Cuts at: 0 327 1539
Size: 327 1212
Enzymes that do cut:
AarI AatII AflIII AlwNI ApaI AscI AvaI BanI
BanII BbeI BbsI BcgI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BssHII BstAPI Bsu36I BtgZI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII HincII
KasI MmeI MscI MslI MspA1I NarI NotI NspI
PasI PflMI PfoI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BplI BsaI BsiWI BsmI BsmBI BspHI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtsI ClaI CspCI DraI DraIII EciI
EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StyI SwaI TaqII XbaI XhoI XmnI