Restriction map for
pFN21AE4124
(Linear) (Six-base) MAPSORT of: KIEE4124.seq Check: 3754 from: 1 to: 1536
KIEE4124.orf
With 182 enzymes: SgfI *
March 22, 2012 17:24 ..
AatII G_ACGT'C
Cuts at: 0 908 1536
Size: 908 628
AfeI AGC'GCT
Cuts at: 0 1249 1466 1536
Size: 1249 217 70
Fragments arranged by size:
1249 217 70
AhdI GACnn_n'nnGTC
Cuts at: 0 271 1536
Size: 271 1265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 988 1536
Size: 604 384 548
Fragments arranged by size:
604 548 384
AlwNI CAG_nnn'CTG
Cuts at: 0 295 994 1536
Size: 295 699 542
Fragments arranged by size:
699 542 295
ApaI G_GGCC'C
Cuts at: 0 1147 1230 1536
Size: 1147 83 306
Fragments arranged by size:
1147 306 83
AvaI C'yCGr_G
Cuts at: 0 261 1171 1312 1536
Size: 261 910 141 224
Fragments arranged by size:
910 261 224 141
BanI G'GyrC_C
Cuts at: 0 709 1204 1536
Size: 709 495 332
Fragments arranged by size:
709 495 332
BanII G_rGCy'C
Cuts at: 0 182 1147 1171 1191 1230 1536
Size: 182 965 24 20 39 306
Fragments arranged by size:
965 306 182 39 24 20
BbeI G_GCGC'C
Cuts at: 0 1208 1536
Size: 1208 328
BbvCI CC'TCA_GC
Cuts at: 0 541 1536
Size: 541 995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 583 617 1165 1199 1536
Size: 583 34 548 34 337
Fragments arranged by size:
583 548 337 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1046 1536
Size: 1046 490
BglII A'GATC_T
Cuts at: 0 828 1536
Size: 828 708
Bme1580I G_kGCm'C
Cuts at: 0 570 1147 1230 1264 1536
Size: 570 577 83 34 272
Fragments arranged by size:
577 570 272 83 34
BmgBI CAC'GTC
Cuts at: 0 333 1536
Size: 333 1203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 1536
Size: 167 32 1337
Fragments arranged by size:
1337 167 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 348 1004 1264 1272 1465 1536
Size: 137 211 656 260 8 193 71
Fragments arranged by size:
656 260 211 193 137 71 8
Bpu10I CC'TnA_GC
Cuts at: 0 541 1536
Size: 541 995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 736 1536
Size: 235 501 800
Fragments arranged by size:
800 501 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 155 464 775 1536
Size: 155 309 311 761
Fragments arranged by size:
761 311 309 155
BsaAI yAC'GTr
Cuts at: 0 1179 1259 1536
Size: 1179 80 277
Fragments arranged by size:
1179 277 80
BsaHI Gr'CG_yC
Cuts at: 0 905 1205 1536
Size: 905 300 331
Fragments arranged by size:
905 331 300
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 737 767 1536
Size: 90 30 617 30 769
Fragments arranged by size:
769 617 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 95 98 256 490 724 793 1330 1362
Size: 95 3 158 234 234 69 537 32
Cuts at: 1362 1536
Size: 174
Fragments arranged by size:
537 234 234 174 158 95 69 32
3
BseYI C'CCAG_C
Cuts at: 0 226 325 1146 1536
Size: 226 99 821 390
Fragments arranged by size:
821 390 226 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 984 1012 1111 1324 1536
Size: 574 410 28 99 213 212
Fragments arranged by size:
574 410 213 212 99 28
BsiHKAI G_wGCw'C
Cuts at: 0 248 447 1137 1178 1258 1532 1536
Size: 248 199 690 41 80 274 4
Fragments arranged by size:
690 274 248 199 80 41 4
Bsp1286I G_dGCh'C
Cuts at: 0 182 248 447 570 1137 1147 1171 1178
Size: 182 66 199 123 567 10 24 7
Cuts at: 1178 1191 1230 1258 1264 1532 1536
Size: 13 39 28 6 268 4
Fragments arranged by size:
567 268 199 182 123 66 39 28
24 13 10 7 6 4
BspHI T'CATG_A
Cuts at: 0 140 1536
Size: 140 1396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 303 1514 1536
Size: 303 1211 22
Fragments arranged by size:
1211 303 22
BsrBI CCG'CTC
Cuts at: 0 1436 1536
Size: 1436 100
BsrDI GCAATG_nn'
Cuts at: 0 873 1536
Size: 873 663
BssSI C'ACGA_G
Cuts at: 0 592 1536
Size: 592 944
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 1536
Size: 318 1218
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 416 1101 1242 1536
Size: 216 200 685 141 294
Fragments arranged by size:
685 294 216 200 141
BstYI r'GATC_y
Cuts at: 0 828 1536
Size: 828 708
Bsu36I CC'TnA_GG
Cuts at: 0 288 736 1536
Size: 288 448 800
Fragments arranged by size:
800 448 288
BtgI C'CryG_G
Cuts at: 0 510 1044 1100 1200 1536
Size: 510 534 56 100 336
Fragments arranged by size:
534 510 336 100 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 429 645 1536
Size: 429 216 891
Fragments arranged by size:
891 429 216
BtsI GCAGTG_nn'
Cuts at: 0 323 490 614 886 1536
Size: 323 167 124 272 650
Fragments arranged by size:
650 323 272 167 124
EaeI y'GGCC_r
Cuts at: 0 6 513 797 810 1047 1219 1536
Size: 6 507 284 13 237 172 317
Fragments arranged by size:
507 317 284 237 172 13 6
EarI CTCTTCn'nnn_
Cuts at: 0 152 467 770 1384 1536
Size: 152 315 303 614 152
Fragments arranged by size:
614 315 303 152 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 348 1004 1264 1272 1465 1536
Size: 137 211 656 260 8 193 71
Fragments arranged by size:
656 260 211 193 137 71 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 1234 1389 1536
Size: 592 642 155 147
Fragments arranged by size:
642 592 155 147
EcoO109I rG'GnC_Cy
Cuts at: 0 38 265 292 703 1143 1226 1227 1278
Size: 38 227 27 411 440 83 1 51
Cuts at: 1278 1536
Size: 258
Fragments arranged by size:
440 411 258 227 83 51 38 27
1
HaeII r_GCGC'y
Cuts at: 0 1094 1208 1251 1468 1536
Size: 1094 114 43 217 68
Fragments arranged by size:
1094 217 114 68 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 257 289 748 780 1536
Size: 185 32 40 32 459 32 756
Fragments arranged by size:
756 459 185 40 32 32 32
KasI G'GCGC_C
Cuts at: 0 1204 1536
Size: 1204 332
MscI TGG'CCA
Cuts at: 0 8 515 812 1049 1221 1536
Size: 8 507 297 237 172 315
Fragments arranged by size:
507 315 297 237 172 8
MslI CAynn'nnrTG
Cuts at: 0 139 1536
Size: 139 1397
MspA1I CmG'CkG
Cuts at: 0 911 950 960 982 1057 1150 1424 1512
Size: 911 39 10 22 75 93 274 88
Cuts at: 1512 1536
Size: 24
Fragments arranged by size:
911 274 93 88 75 39 24 22
10
NarI GG'CG_CC
Cuts at: 0 1205 1536
Size: 1205 331
NcoI C'CATG_G
Cuts at: 0 1100 1200 1536
Size: 1100 100 336
Fragments arranged by size:
1100 336 100
NspI r_CATG'y
Cuts at: 0 138 499 796 854 1536
Size: 138 361 297 58 682
Fragments arranged by size:
682 361 297 138 58
PasI CC'CwG_GG
Cuts at: 0 571 1536
Size: 571 965
PmlI CAC'GTG
Cuts at: 0 1179 1259 1536
Size: 1179 80 277
Fragments arranged by size:
1179 277 80
PpuMI rG'GwC_Cy
Cuts at: 0 265 292 1278 1536
Size: 265 27 986 258
Fragments arranged by size:
986 265 258 27
PshAI GACnn'nnGTC
Cuts at: 0 456 1536
Size: 456 1080
PspOMI G'GGCC_C
Cuts at: 0 1143 1226 1536
Size: 1143 83 310
Fragments arranged by size:
1143 310 83
PstI C_TGCA'G
Cuts at: 0 609 959 1142 1155 1352 1412 1511 1536
Size: 609 350 183 13 197 60 99 25
Fragments arranged by size:
609 350 197 183 99 60 25 13
PvuII CAG'CTG
Cuts at: 0 960 982 1057 1150 1512 1536
Size: 960 22 75 93 362 24
Fragments arranged by size:
960 362 93 75 24 22
RsrII CG'GwC_CG
Cuts at: 0 764 1536
Size: 764 772
SanDI GG'GwC_CC
Cuts at: 0 265 1536
Size: 265 1271
ScaI AGT'ACT
Cuts at: 0 369 1536
Size: 369 1167
SfcI C'TryA_G
Cuts at: 0 605 955 1138 1151 1293 1348 1408 1507
Size: 605 350 183 13 142 55 60 99
Cuts at: 1507 1536
Size: 29
Fragments arranged by size:
605 350 183 142 99 60 55 29
13
SfoI GGC'GCC
Cuts at: 0 1206 1536
Size: 1206 330
SmaI CCC'GGG
Cuts at: 0 263 1536
Size: 263 1273
SmlI C'TyrA_G
Cuts at: 0 250 715 1536
Size: 250 465 821
Fragments arranged by size:
821 465 250
StuI AGG'CCT
Cuts at: 0 598 1536
Size: 598 938
StyI C'CwwG_G
Cuts at: 0 1100 1200 1235 1385 1536
Size: 1100 100 35 150 151
Fragments arranged by size:
1100 151 150 100 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 752 1536
Size: 752 784
TatI w'GTAC_w
Cuts at: 0 367 1536
Size: 367 1169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 737 769 1536
Size: 737 32 767
Fragments arranged by size:
767 737 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 1536
Size: 372 1164
XmaI C'CCGG_G
Cuts at: 0 261 1536
Size: 261 1275
ZraI GAC'GTC
Cuts at: 0 906 1536
Size: 906 630
Enzymes that do cut:
AatII AfeI AhdI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BcgI BglI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PasI
PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SanDI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BlpI BmrI
BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PpiI
PsiI PspXI PsrI PvuI SacI SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI