Restriction map for
pFN21AE4123
(Linear) (Six-base) MAPSORT of: KIEE4123.seq Check: 6270 from: 1 to: 1533
KIEE4123.orf
With 182 enzymes: SgfI *
July 3, 2012 18:37 ..
AccI GT'mk_AC
Cuts at: 0 826 1178 1533
Size: 826 352 355
Fragments arranged by size:
826 355 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 882 1123 1533
Size: 760 122 241 410
Fragments arranged by size:
760 410 241 122
AfeI AGC'GCT
Cuts at: 0 639 1533
Size: 639 894
AflIII A'CryG_T
Cuts at: 0 1001 1277 1533
Size: 1001 276 256
Fragments arranged by size:
1001 276 256
AlwNI CAG_nnn'CTG
Cuts at: 0 226 1533
Size: 226 1307
ApoI r'AATT_y
Cuts at: 0 449 1533
Size: 449 1084
AvaI C'yCGr_G
Cuts at: 0 1090 1533
Size: 1090 443
BanI G'GyrC_C
Cuts at: 0 455 1533
Size: 455 1078
BbeI G_GCGC'C
Cuts at: 0 459 1533
Size: 459 1074
BbsI GAAGACnn'nnnn_
Cuts at: 0 1035 1533
Size: 1035 498
BbvCI CC'TCA_GC
Cuts at: 0 1187 1533
Size: 1187 346
BclI T'GATC_A
Cuts at: 0 263 1533
Size: 263 1270
BglI GCCn_nnn'nGGC
Cuts at: 0 113 1533
Size: 113 1420
Bme1580I G_kGCm'C
Cuts at: 0 993 1285 1315 1533
Size: 993 292 30 218
Fragments arranged by size:
993 292 218 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 401 1533
Size: 401 1132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1177 1533
Size: 1177 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1152 1533
Size: 724 428 381
Fragments arranged by size:
724 428 381
Bpu10I CC'TnA_GC
Cuts at: 0 227 1187 1533
Size: 227 960 346
Fragments arranged by size:
960 346 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1449 1533
Size: 1449 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 332 666 746 1533
Size: 286 46 334 80 787
Fragments arranged by size:
787 334 286 80 46
BsaBI GATnn'nnATC
Cuts at: 0 154 1533
Size: 154 1379
BsaHI Gr'CG_yC
Cuts at: 0 456 1108 1533
Size: 456 652 425
Fragments arranged by size:
652 456 425
BsaWI w'CCGG_w
Cuts at: 0 863 1533
Size: 863 670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 913 943 1111 1141 1533
Size: 913 30 168 30 392
Fragments arranged by size:
913 392 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 1533
Size: 601 932
BseYI C'CCAG_C
Cuts at: 0 277 296 459 510 880 1533
Size: 277 19 163 51 370 653
Fragments arranged by size:
653 370 277 163 51 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1193 1533
Size: 1193 340
BsiHKAI G_wGCw'C
Cuts at: 0 863 1533
Size: 863 670
BsmI GAATG_Cn'
Cuts at: 0 1220 1533
Size: 1220 313
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 1533
Size: 172 1361
Bsp1286I G_dGCh'C
Cuts at: 0 863 993 1285 1315 1533
Size: 863 130 292 30 218
Fragments arranged by size:
863 292 218 130 30
BspHI T'CATG_A
Cuts at: 0 908 1533
Size: 908 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 838 1533
Size: 838 695
BsrDI GCAATG_nn'
Cuts at: 0 272 1325 1343 1533
Size: 272 1053 18 190
Fragments arranged by size:
1053 272 190 18
BsrFI r'CCGG_y
Cuts at: 0 116 401 1533
Size: 116 285 1132
Fragments arranged by size:
1132 285 116
BstEII G'GTnAC_C
Cuts at: 0 1248 1533
Size: 1248 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 1533
Size: 442 1091
BstYI r'GATC_y
Cuts at: 0 149 1085 1533
Size: 149 936 448
Fragments arranged by size:
936 448 149
Bsu36I CC'TnA_GG
Cuts at: 0 1018 1533
Size: 1018 515
BtgI C'CryG_G
Cuts at: 0 524 656 1533
Size: 524 132 877
Fragments arranged by size:
877 524 132
BtsI GCAGTG_nn'
Cuts at: 0 571 709 1250 1533
Size: 571 138 541 283
Fragments arranged by size:
571 541 283 138
DraIII CAC_nnn'GTG
Cuts at: 0 1319 1533
Size: 1319 214
EaeI y'GGCC_r
Cuts at: 0 653 1010 1533
Size: 653 357 523
Fragments arranged by size:
653 523 357
EarI CTCTTCn'nnn_
Cuts at: 0 65 1150 1168 1533
Size: 65 1085 18 365
Fragments arranged by size:
1085 365 65 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 1533
Size: 1018 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 760 882 1123 1152 1533
Size: 724 36 122 241 29 381
Fragments arranged by size:
724 381 241 122 36 29
EcoO109I rG'GnC_Cy
Cuts at: 0 223 1335 1533
Size: 223 1112 198
Fragments arranged by size:
1112 223 198
EcoRV GAT'ATC
Cuts at: 0 480 1533
Size: 480 1053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 1533
Size: 909 624
FseI GG_CCGG'CC
Cuts at: 0 120 1533
Size: 120 1413
HaeII r_GCGC'y
Cuts at: 0 459 641 1533
Size: 459 182 892
Fragments arranged by size:
892 459 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 481 513 913 945 1189 1221
Size: 82 32 367 32 400 32 244 32
Cuts at: 1221 1533
Size: 312
Fragments arranged by size:
400 367 312 244 82 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 193 1533
Size: 193 1340
KasI G'GCGC_C
Cuts at: 0 455 1533
Size: 455 1078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1397 1533
Size: 164 1233 136
Fragments arranged by size:
1233 164 136
MscI TGG'CCA
Cuts at: 0 1012 1533
Size: 1012 521
MspA1I CmG'CkG
Cuts at: 0 258 1533
Size: 258 1275
NaeI GCC'GGC
Cuts at: 0 118 1533
Size: 118 1415
NarI GG'CG_CC
Cuts at: 0 456 1533
Size: 456 1077
NcoI C'CATG_G
Cuts at: 0 524 1533
Size: 524 1009
NgoMIV G'CCGG_C
Cuts at: 0 116 1533
Size: 116 1417
NspI r_CATG'y
Cuts at: 0 1005 1281 1533
Size: 1005 276 252
Fragments arranged by size:
1005 276 252
PasI CC'CwG_GG
Cuts at: 0 220 1294 1533
Size: 220 1074 239
Fragments arranged by size:
1074 239 220
PciI A'CATG_T
Cuts at: 0 1001 1277 1533
Size: 1001 276 256
Fragments arranged by size:
1001 276 256
PfoI T'CCnGG_A
Cuts at: 0 198 536 722 971 1533
Size: 198 338 186 249 562
Fragments arranged by size:
562 338 249 198 186
PpuMI rG'GwC_Cy
Cuts at: 0 1335 1533
Size: 1335 198
PstI C_TGCA'G
Cuts at: 0 1085 1533
Size: 1085 448
SapI GCTCTTCn'nnn_
Cuts at: 0 65 1533
Size: 65 1468
SfcI C'TryA_G
Cuts at: 0 1081 1262 1533
Size: 1081 181 271
Fragments arranged by size:
1081 271 181
SfoI GGC'GCC
Cuts at: 0 457 1533
Size: 457 1076
SmlI C'TyrA_G
Cuts at: 0 1464 1533
Size: 1464 69
StyI C'CwwG_G
Cuts at: 0 524 1073 1533
Size: 524 549 460
Fragments arranged by size:
549 524 460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 699 1119 1533
Size: 699 420 414
Fragments arranged by size:
699 420 414
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApoI AvaI BanI
BbeI BbsI BbvCI BclI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV
FalI FseI HaeII Hin4I HindIII KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PpuMI PstI SapI SfcI SfoI SmlI StyI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BcgI BciVI BfrBI BglII BlpI
BmgBI BmtI BsaAI BsiEI BsiWI BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EcoICRI EcoNI EcoRI FspI FspAI
HincII HpaI KpnI MfeI MluI MslI NdeI NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI