Restriction map for
pFN21AE4122
(Linear) (Six-base) MAPSORT of: KIEE4122.seq Check: 4837 from: 1 to: 1515
KIEE4122.orf
With 182 enzymes: SgfI *
March 22, 2012 17:24 ..
AatII G_ACGT'C
Cuts at: 0 362 1515
Size: 362 1153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1167 1515
Size: 172 995 348
Fragments arranged by size:
995 348 172
AfeI AGC'GCT
Cuts at: 0 39 1515
Size: 39 1476
AflIII A'CryG_T
Cuts at: 0 1328 1481 1515
Size: 1328 153 34
Fragments arranged by size:
1328 153 34
ApaI G_GGCC'C
Cuts at: 0 525 1515
Size: 525 990
ApoI r'AATT_y
Cuts at: 0 111 562 878 1515
Size: 111 451 316 637
Fragments arranged by size:
637 451 316 111
AseI AT'TA_AT
Cuts at: 0 932 1515
Size: 932 583
BanII G_rGCy'C
Cuts at: 0 525 1515
Size: 525 990
BbsI GAAGACnn'nnnn_
Cuts at: 0 954 1515
Size: 954 561
BciVI GTATCCnnnnn_n'
Cuts at: 0 780 1515
Size: 780 735
BclI T'GATC_A
Cuts at: 0 1346 1515
Size: 1346 169
BglII A'GATC_T
Cuts at: 0 866 1364 1515
Size: 866 498 151
Fragments arranged by size:
866 498 151
BlpI GC'TnA_GC
Cuts at: 0 41 1515
Size: 41 1474
Bme1580I G_kGCm'C
Cuts at: 0 525 1515
Size: 525 990
BmgBI CAC'GTC
Cuts at: 0 1005 1515
Size: 1005 510
BmrI ACTGGGnnnn_n'
Cuts at: 0 336 1437 1515
Size: 336 1101 78
Fragments arranged by size:
1101 336 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 564 678 1515
Size: 153 411 114 837
Fragments arranged by size:
837 411 153 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1459 1515
Size: 1004 455 56
Fragments arranged by size:
1004 455 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 1396 1515
Size: 1396 119
BsaAI yAC'GTr
Cuts at: 0 1329 1515
Size: 1329 186
BsaHI Gr'CG_yC
Cuts at: 0 359 1515
Size: 359 1156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 1515
Size: 452 30 1033
Fragments arranged by size:
1033 452 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 48 77 711 1384 1390 1515
Size: 48 29 634 673 6 125
Fragments arranged by size:
673 634 125 48 29 6
BseYI C'CCAG_C
Cuts at: 0 401 681 1515
Size: 401 280 834
Fragments arranged by size:
834 401 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 1355 1515
Size: 1248 107 160
Fragments arranged by size:
1248 160 107
BsiHKAI G_wGCw'C
Cuts at: 0 78 1515
Size: 78 1437
BsmI GAATG_Cn'
Cuts at: 0 56 1515
Size: 56 1459
Bsp1286I G_dGCh'C
Cuts at: 0 78 525 1515
Size: 78 447 990
Fragments arranged by size:
990 447 78
BspHI T'CATG_A
Cuts at: 0 177 1515
Size: 177 1338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1158 1214 1244 1515
Size: 1158 56 30 271
Fragments arranged by size:
1158 271 56 30
BsrGI T'GTAC_A
Cuts at: 0 858 1515
Size: 858 657
BstYI r'GATC_y
Cuts at: 0 497 866 1364 1515
Size: 497 369 498 151
Fragments arranged by size:
498 497 369 151
Bsu36I CC'TnA_GG
Cuts at: 0 433 1515
Size: 433 1082
BtgI C'CryG_G
Cuts at: 0 97 1515
Size: 97 1418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 233 1515
Size: 233 1282
BtsI GCAGTG_nn'
Cuts at: 0 399 1515
Size: 399 1116
DrdI GACnn_nn'nnGTC
Cuts at: 0 1014 1515
Size: 1014 501
EaeI y'GGCC_r
Cuts at: 0 2 622 1515
Size: 2 620 893
Fragments arranged by size:
893 620 2
EarI CTCTTCn'nnn_
Cuts at: 0 25 224 977 1136 1262 1515
Size: 25 199 753 159 126 253
Fragments arranged by size:
753 253 199 159 126 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 889 1261 1515
Size: 889 372 254
Fragments arranged by size:
889 372 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 172 564 678 1167 1515
Size: 153 19 392 114 489 348
Fragments arranged by size:
489 392 348 153 114 19
EcoO109I rG'GnC_Cy
Cuts at: 0 521 522 1515
Size: 521 1 993
Fragments arranged by size:
993 521 1
EcoRI G'AATT_C
Cuts at: 0 878 1515
Size: 878 637
HaeII r_GCGC'y
Cuts at: 0 41 1515
Size: 41 1474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 226 258 489 521 813 845
Size: 119 32 75 32 231 32 292 32
Cuts at: 845 1515
Size: 670
Fragments arranged by size:
670 292 231 119 75 32 32 32
32
HincII GTy'rAC
Cuts at: 0 138 347 707 1515
Size: 138 209 360 808
Fragments arranged by size:
808 360 209 138
MscI TGG'CCA
Cuts at: 0 624 1515
Size: 624 891
MspA1I CmG'CkG
Cuts at: 0 7 405 717 1223 1515
Size: 7 398 312 506 292
Fragments arranged by size:
506 398 312 292 7
NspI r_CATG'y
Cuts at: 0 431 1029 1485 1515
Size: 431 598 456 30
Fragments arranged by size:
598 456 431 30
PasI CC'CwG_GG
Cuts at: 0 635 1515
Size: 635 880
PciI A'CATG_T
Cuts at: 0 1481 1515
Size: 1481 34
PfoI T'CCnGG_A
Cuts at: 0 539 1085 1515
Size: 539 546 430
Fragments arranged by size:
546 539 430
PshAI GACnn'nnGTC
Cuts at: 0 666 1515
Size: 666 849
PspOMI G'GGCC_C
Cuts at: 0 521 1515
Size: 521 994
PstI C_TGCA'G
Cuts at: 0 291 716 1211 1515
Size: 291 425 495 304
Fragments arranged by size:
495 425 304 291
PvuII CAG'CTG
Cuts at: 0 405 1515
Size: 405 1110
SapI GCTCTTCn'nnn_
Cuts at: 0 1136 1515
Size: 1136 379
SexAI A'CCwGG_T
Cuts at: 0 938 1515
Size: 938 577
SfcI C'TryA_G
Cuts at: 0 287 712 792 1207 1405 1515
Size: 287 425 80 415 198 110
Fragments arranged by size:
425 415 287 198 110 80
SmlI C'TyrA_G
Cuts at: 0 1019 1474 1515
Size: 1019 455 41
Fragments arranged by size:
1019 455 41
SspI AAT'ATT
Cuts at: 0 733 1515
Size: 733 782
StuI AGG'CCT
Cuts at: 0 166 1515
Size: 166 1349
StyI C'CwwG_G
Cuts at: 0 817 1515
Size: 817 698
TatI w'GTAC_w
Cuts at: 0 858 1478 1515
Size: 858 620 37
Fragments arranged by size:
858 620 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 183 215 236 268 452 484 1515
Size: 183 32 21 32 184 32 1031
Fragments arranged by size:
1031 184 183 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 940 1515
Size: 940 575
XbaI T'CTAG_A
Cuts at: 0 300 1515
Size: 300 1215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 1515
Size: 910 605
XmnI GAAnn'nnTTC
Cuts at: 0 878 1515
Size: 878 637
ZraI GAC'GTC
Cuts at: 0 360 1515
Size: 360 1155
Enzymes that do cut:
AatII AcuI AfeI AflIII ApaI ApoI AseI BanII
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MscI MspA1I
NspI PasI PciI PfoI PshAI PspOMI PstI PvuII
SapI SexAI SfcI SmlI SspI StuI StyI TatI
TstI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TaqII XhoI XmaI