Restriction map for
pFN21AE4120
(Linear) (Six-base) MAPSORT of: KIEE4120.seq Check: 9553 from: 1 to: 1509
KIEE4120.orf
With 182 enzymes: SgfI *
July 3, 2012 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1129 1509
Size: 1129 380
AccI GT'mk_AC
Cuts at: 0 1228 1365 1509
Size: 1228 137 144
Fragments arranged by size:
1228 144 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 863 1509
Size: 24 839 646
Fragments arranged by size:
839 646 24
AflIII A'CryG_T
Cuts at: 0 179 1509
Size: 179 1330
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1509
Size: 363 1146
AseI AT'TA_AT
Cuts at: 0 386 1509
Size: 386 1123
AvaI C'yCGr_G
Cuts at: 0 1415 1509
Size: 1415 94
BanII G_rGCy'C
Cuts at: 0 135 629 1486 1509
Size: 135 494 857 23
Fragments arranged by size:
857 494 135 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 1509
Size: 869 640
BbvCI CC'TCA_GC
Cuts at: 0 395 1427 1509
Size: 395 1032 82
Fragments arranged by size:
1032 395 82
BglI GCCn_nnn'nGGC
Cuts at: 0 892 935 1509
Size: 892 43 574
Fragments arranged by size:
892 574 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 1508 1509
Size: 1508 1
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 431 769 803 1267 1393 1509
Size: 385 46 338 34 464 126 116
Fragments arranged by size:
464 385 338 126 116 46 34
Bpu10I CC'TnA_GC
Cuts at: 0 55 395 487 1004 1427 1509
Size: 55 340 92 517 423 82
Fragments arranged by size:
517 423 340 92 82 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 435 1509
Size: 435 1074
BsaWI w'CCGG_w
Cuts at: 0 1100 1509
Size: 1100 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 875 905 1375 1405 1446 1476 1509
Size: 875 30 470 30 41 30 33
Fragments arranged by size:
875 470 41 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 766 1509
Size: 586 180 743
Fragments arranged by size:
743 586 180
BseYI C'CCAG_C
Cuts at: 0 1012 1509
Size: 1012 497
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1509
Size: 821 688
BsmI GAATG_Cn'
Cuts at: 0 371 1509
Size: 371 1138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 94 1120 1509
Size: 94 1026 389
Fragments arranged by size:
1026 389 94
Bsp1286I G_dGCh'C
Cuts at: 0 135 629 1486 1509
Size: 135 494 857 23
Fragments arranged by size:
857 494 135 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1129 1509
Size: 1129 380
BsrBI CCG'CTC
Cuts at: 0 482 1092 1509
Size: 482 610 417
Fragments arranged by size:
610 482 417
BsrDI GCAATG_nn'
Cuts at: 0 1043 1509
Size: 1043 466
BsrFI r'CCGG_y
Cuts at: 0 518 1509
Size: 518 991
BsrGI T'GTAC_A
Cuts at: 0 352 1509
Size: 352 1157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1130 1509
Size: 1130 379
BstYI r'GATC_y
Cuts at: 0 359 1509
Size: 359 1150
BstZ17I GTA'TAC
Cuts at: 0 1229 1366 1509
Size: 1229 137 143
Fragments arranged by size:
1229 143 137
Bsu36I CC'TnA_GG
Cuts at: 0 569 1509
Size: 569 940
BtgI C'CryG_G
Cuts at: 0 684 818 840 1509
Size: 684 134 22 669
Fragments arranged by size:
684 669 134 22
BtsI GCAGTG_nn'
Cuts at: 0 915 945 1509
Size: 915 30 564
Fragments arranged by size:
915 564 30
DraIII CAC_nnn'GTG
Cuts at: 0 141 1509
Size: 141 1368
EaeI y'GGCC_r
Cuts at: 0 898 1509
Size: 898 611
EarI CTCTTCn'nnn_
Cuts at: 0 1022 1427 1445 1509
Size: 1022 405 18 64
Fragments arranged by size:
1022 405 64 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 285 694 1509
Size: 285 409 815
Fragments arranged by size:
815 409 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 385 431 769 803 863 1267 1393
Size: 24 361 46 338 34 60 404 126
Cuts at: 1393 1509
Size: 116
Fragments arranged by size:
404 361 338 126 116 60 46 34
24
EcoO109I rG'GnC_Cy
Cuts at: 0 1378 1509
Size: 1378 131
EcoRV GAT'ATC
Cuts at: 0 294 549 1509
Size: 294 255 960
Fragments arranged by size:
960 294 255
FspI TGC'GCA
Cuts at: 0 76 1509
Size: 76 1433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 417 449 1420 1446 1452 1478
Size: 166 32 219 32 971 26 6 26
Cuts at: 1478 1509
Size: 31
Fragments arranged by size:
971 219 166 32 32 31 26 26
6
HincII GTy'rAC
Cuts at: 0 918 1509
Size: 918 591
HindIII A'AGCT_T
Cuts at: 0 122 1509
Size: 122 1387
MfeI C'AATT_G
Cuts at: 0 22 1509
Size: 22 1487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1509
Size: 673 836
MslI CAynn'nnrTG
Cuts at: 0 1292 1298 1509
Size: 1292 6 211
Fragments arranged by size:
1292 211 6
MspA1I CmG'CkG
Cuts at: 0 313 1245 1509
Size: 313 932 264
Fragments arranged by size:
932 313 264
NcoI C'CATG_G
Cuts at: 0 818 840 1509
Size: 818 22 669
Fragments arranged by size:
818 669 22
NspI r_CATG'y
Cuts at: 0 81 183 1176 1215 1509
Size: 81 102 993 39 294
Fragments arranged by size:
993 294 102 81 39
PciI A'CATG_T
Cuts at: 0 179 1509
Size: 179 1330
PfoI T'CCnGG_A
Cuts at: 0 170 362 1509
Size: 170 192 1147
Fragments arranged by size:
1147 192 170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1509
Size: 1081 32 396
Fragments arranged by size:
1081 396 32
PvuII CAG'CTG
Cuts at: 0 313 1245 1509
Size: 313 932 264
Fragments arranged by size:
932 313 264
RsrII CG'GwC_CG
Cuts at: 0 681 1509
Size: 681 828
SfcI C'TryA_G
Cuts at: 0 848 1509
Size: 848 661
SmlI C'TyrA_G
Cuts at: 0 1415 1509
Size: 1415 94
SphI G_CATG'C
Cuts at: 0 81 1509
Size: 81 1428
StyI C'CwwG_G
Cuts at: 0 818 840 887 1509
Size: 818 22 47 622
Fragments arranged by size:
818 622 47 22
TatI w'GTAC_w
Cuts at: 0 199 352 726 1509
Size: 199 153 374 783
Fragments arranged by size:
783 374 199 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 875 907 1375 1407 1509
Size: 875 32 468 32 102
Fragments arranged by size:
875 468 102 32 32
XhoI C'TCGA_G
Cuts at: 0 1415 1509
Size: 1415 94
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AseI AvaI BanII
BbsI BbvCI BglI BmrI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
EcoRV FspI Hin4I HincII HindIII MfeI MmeI MslI
MspA1I NcoI NspI PciI PfoI PpiI PvuII RsrII
SfcI SmlI SphI StyI TatI TstI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI BspEI BspHI BssHII
BssSI BstBI BstEII BstXI BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspAI
HaeII HpaI KasI KpnI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XmaI XmnI ZraI