Restriction map for
pFN21AE4115
(Linear) (Six-base) MAPSORT of: KIEE4115.seq Check: 8227 from: 1 to: 1464
KIEE4115.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1464
Size: 472 992
AflIII A'CryG_T
Cuts at: 0 386 1464
Size: 386 1078
AhdI GACnn_n'nnGTC
Cuts at: 0 1448 1464
Size: 1448 16
AlwNI CAG_nnn'CTG
Cuts at: 0 101 687 1464
Size: 101 586 777
Fragments arranged by size:
777 586 101
ApaI G_GGCC'C
Cuts at: 0 262 1464
Size: 262 1202
BamHI G'GATC_C
Cuts at: 0 545 1100 1464
Size: 545 555 364
Fragments arranged by size:
555 545 364
BanII G_rGCy'C
Cuts at: 0 62 262 698 840 1039 1464
Size: 62 200 436 142 199 425
Fragments arranged by size:
436 425 200 199 142 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 1096 1464
Size: 1096 368
BciVI GTATCCnnnnn_n'
Cuts at: 0 1192 1464
Size: 1192 272
BglI GCCn_nnn'nGGC
Cuts at: 0 213 1464
Size: 213 1251
BglII A'GATC_T
Cuts at: 0 843 1464
Size: 843 621
Bme1580I G_kGCm'C
Cuts at: 0 262 1286 1464
Size: 262 1024 178
Fragments arranged by size:
1024 262 178
BmgBI CAC'GTC
Cuts at: 0 906 1464
Size: 906 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 675 906 1077 1135 1212 1464
Size: 675 231 171 58 77 252
Fragments arranged by size:
675 252 231 171 77 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 1464
Size: 717 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 730 1161 1464
Size: 76 654 431 303
Fragments arranged by size:
654 431 303 76
Bpu10I CC'TnA_GC
Cuts at: 0 76 1464
Size: 76 1388
BsaI GGTCTCn'nnnn_
Cuts at: 0 368 1464
Size: 368 1096
BsaWI w'CCGG_w
Cuts at: 0 956 1464
Size: 956 508
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 971 1001 1464
Size: 971 30 463
Fragments arranged by size:
971 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 475 1041 1464
Size: 415 60 566 423
Fragments arranged by size:
566 423 415 60
BseYI C'CCAG_C
Cuts at: 0 1040 1464
Size: 1040 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1464
Size: 311 1153
BsiHKAI G_wGCw'C
Cuts at: 0 62 374 698 1464
Size: 62 312 324 766
Fragments arranged by size:
766 324 312 62
Bsp1286I G_dGCh'C
Cuts at: 0 62 262 374 698 840 1039 1286 1464
Size: 62 200 112 324 142 199 247 178
Fragments arranged by size:
324 247 200 199 178 142 112 62
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 209 1464
Size: 49 160 1255
Fragments arranged by size:
1255 160 49
BsrBI CCG'CTC
Cuts at: 0 360 1464
Size: 360 1104
BssSI C'ACGA_G
Cuts at: 0 394 1464
Size: 394 1070
BstEII G'GTnAC_C
Cuts at: 0 36 379 1464
Size: 36 343 1085
Fragments arranged by size:
1085 343 36
BstYI r'GATC_y
Cuts at: 0 545 705 843 1100 1464
Size: 545 160 138 257 364
Fragments arranged by size:
545 364 257 160 138
Bsu36I CC'TnA_GG
Cuts at: 0 790 1464
Size: 790 674
BtgI C'CryG_G
Cuts at: 0 297 375 1353 1464
Size: 297 78 978 111
Fragments arranged by size:
978 297 111 78
DraI TTT'AAA
Cuts at: 0 935 1464
Size: 935 529
DrdI GACnn_nn'nnGTC
Cuts at: 0 1001 1027 1464
Size: 1001 26 437
Fragments arranged by size:
1001 437 26
EaeI y'GGCC_r
Cuts at: 0 90 221 1082 1464
Size: 90 131 861 382
Fragments arranged by size:
861 382 131 90
EarI CTCTTCn'nnn_
Cuts at: 0 26 322 617 644 1464
Size: 26 296 295 27 820
Fragments arranged by size:
820 296 295 27 26
EcoICRI GAG'CTC
Cuts at: 0 60 696 1464
Size: 60 636 768
Fragments arranged by size:
768 636 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 472 730 1161 1464
Size: 76 396 258 431 303
Fragments arranged by size:
431 396 303 258 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 1464
Size: 397 1067
EcoO109I rG'GnC_Cy
Cuts at: 0 259 1129 1238 1464
Size: 259 870 109 226
Fragments arranged by size:
870 259 226 109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 301 1464
Size: 301 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 530 562 683 715 900 932 1434 1464
Size: 530 32 121 32 185 32 502 30
Fragments arranged by size:
530 502 185 121 32 32 32 30
HincII GTy'rAC
Cuts at: 0 731 903 1464
Size: 731 172 561
Fragments arranged by size:
731 561 172
MscI TGG'CCA
Cuts at: 0 92 223 1084 1464
Size: 92 131 861 380
Fragments arranged by size:
861 380 131 92
MslI CAynn'nnrTG
Cuts at: 0 1226 1279 1464
Size: 1226 53 185
Fragments arranged by size:
1226 185 53
MspA1I CmG'CkG
Cuts at: 0 273 567 1464
Size: 273 294 897
Fragments arranged by size:
897 294 273
NcoI C'CATG_G
Cuts at: 0 297 1353 1464
Size: 297 1056 111
Fragments arranged by size:
1056 297 111
PasI CC'CwG_GG
Cuts at: 0 262 1464
Size: 262 1202
PfoI T'CCnGG_A
Cuts at: 0 511 1464
Size: 511 953
PpuMI rG'GwC_Cy
Cuts at: 0 1129 1464
Size: 1129 335
PshAI GACnn'nnGTC
Cuts at: 0 883 1464
Size: 883 581
PsiI TTA'TAA
Cuts at: 0 1340 1464
Size: 1340 124
PspOMI G'GGCC_C
Cuts at: 0 258 1464
Size: 258 1206
PstI C_TGCA'G
Cuts at: 0 314 749 1464
Size: 314 435 715
Fragments arranged by size:
715 435 314
PvuII CAG'CTG
Cuts at: 0 273 567 1464
Size: 273 294 897
Fragments arranged by size:
897 294 273
SacI G_AGCT'C
Cuts at: 0 62 698 1464
Size: 62 636 766
Fragments arranged by size:
766 636 62
SapI GCTCTTCn'nnn_
Cuts at: 0 322 1464
Size: 322 1142
ScaI AGT'ACT
Cuts at: 0 14 1464
Size: 14 1450
SfcI C'TryA_G
Cuts at: 0 310 745 1023 1464
Size: 310 435 278 441
Fragments arranged by size:
441 435 310 278
SspI AAT'ATT
Cuts at: 0 1384 1464
Size: 1384 80
StyI C'CwwG_G
Cuts at: 0 297 1353 1464
Size: 297 1056 111
Fragments arranged by size:
1056 297 111
SwaI ATTT'AAAT
Cuts at: 0 935 1464
Size: 935 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1194 1464
Size: 1194 270
TatI w'GTAC_w
Cuts at: 0 12 1464
Size: 12 1452
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1190 1222 1464
Size: 1190 32 242
Fragments arranged by size:
1190 242 32
Tth111I GACn'n_nGTC
Cuts at: 0 681 1464
Size: 681 783
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI BamHI BanII BbsI
BciVI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BssSI BstEII BstYI Bsu36I BtgI
DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MscI MslI MspA1I NcoI PasI
PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SapI ScaI SfcI SspI StyI SwaI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BclI
BfrBI BlpI BmtI BpuEI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraIII EagI EciI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI XbaI XcmI XhoI XmaI
XmnI ZraI