Restriction map for
pFN21AE4114
(Linear) (Six-base) MAPSORT of: KIEE4114.seq Check: 7629 from: 1 to: 1461
KIEE4114.orf
With 182 enzymes: SgfI *
July 3, 2012 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1015 1375 1461
Size: 1015 360 86
Fragments arranged by size:
1015 360 86
AccI GT'mk_AC
Cuts at: 0 383 1461
Size: 383 1078
Acc65I G'GTAC_C
Cuts at: 0 1206 1461
Size: 1206 255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 448 495 1186 1461
Size: 25 423 47 691 275
Fragments arranged by size:
691 423 275 47 25
AfeI AGC'GCT
Cuts at: 0 991 1461
Size: 991 470
AleI CACnn'nnGTG
Cuts at: 0 602 943 1461
Size: 602 341 518
Fragments arranged by size:
602 518 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 1411 1461
Size: 211 1200 50
Fragments arranged by size:
1200 211 50
AlwNI CAG_nnn'CTG
Cuts at: 0 543 1007 1461
Size: 543 464 454
Fragments arranged by size:
543 464 454
ApaLI G'TGCA_C
Cuts at: 0 268 1461
Size: 268 1193
AvaI C'yCGr_G
Cuts at: 0 653 1138 1202 1441 1461
Size: 653 485 64 239 20
Fragments arranged by size:
653 485 239 64 20
BanI G'GyrC_C
Cuts at: 0 1082 1206 1461
Size: 1082 124 255
Fragments arranged by size:
1082 255 124
BanII G_rGCy'C
Cuts at: 0 77 257 317 346 407 458 926 1247
Size: 77 180 60 29 61 51 468 321
Cuts at: 1247 1461
Size: 214
Fragments arranged by size:
468 321 214 180 77 61 60 51
29
BbeI G_GCGC'C
Cuts at: 0 1086 1461
Size: 1086 375
BbsI GAAGACnn'nnnn_
Cuts at: 0 57 501 1461
Size: 57 444 960
Fragments arranged by size:
960 444 57
BbvCI CC'TCA_GC
Cuts at: 0 1435 1461
Size: 1435 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1174 1208 1461
Size: 1174 34 253
Fragments arranged by size:
1174 253 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1118 1422 1461
Size: 1118 304 39
Fragments arranged by size:
1118 304 39
BlpI GC'TnA_GC
Cuts at: 0 286 1461
Size: 286 1175
Bme1580I G_kGCm'C
Cuts at: 0 272 1105 1461
Size: 272 833 356
Fragments arranged by size:
833 356 272
BmrI ACTGGGnnnn_n'
Cuts at: 0 1059 1461
Size: 1059 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 79 601 655 729 940 1461
Size: 49 30 522 54 74 211 521
Fragments arranged by size:
522 521 211 74 54 49 30
Bpu10I CC'TnA_GC
Cuts at: 0 986 1435 1461
Size: 986 449 26
Fragments arranged by size:
986 449 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1461
Size: 871 590
BsaHI Gr'CG_yC
Cuts at: 0 1083 1461
Size: 1083 378
BsaWI w'CCGG_w
Cuts at: 0 275 479 722 857 1461
Size: 275 204 243 135 604
Fragments arranged by size:
604 275 243 204 135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 577 1461
Size: 415 162 884
Fragments arranged by size:
884 415 162
BseYI C'CCAG_C
Cuts at: 0 339 515 965 1417 1461
Size: 339 176 450 452 44
Fragments arranged by size:
452 450 339 176 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 967 1237 1461
Size: 529 438 270 224
Fragments arranged by size:
529 438 270 224
BsiEI CG_ry'CG
Cuts at: 0 1041 1293 1312 1461
Size: 1041 252 19 149
Fragments arranged by size:
1041 252 149 19
BsiHKAI G_wGCw'C
Cuts at: 0 272 317 407 458 926 1145 1461
Size: 272 45 90 51 468 219 316
Fragments arranged by size:
468 316 272 219 90 51 45
BsmI GAATG_Cn'
Cuts at: 0 784 1461
Size: 784 677
BsmBI CGTCTCn'nnnn_
Cuts at: 0 210 378 1461
Size: 210 168 1083
Fragments arranged by size:
1083 210 168
Bsp1286I G_dGCh'C
Cuts at: 0 77 257 272 317 346 407 458 926
Size: 77 180 15 45 29 61 51 468
Cuts at: 926 1105 1145 1247 1461
Size: 179 40 102 214
Fragments arranged by size:
468 214 180 179 102 77 61 51
45 40 29 15
BspEI T'CCGG_A
Cuts at: 0 722 857 1461
Size: 722 135 604
Fragments arranged by size:
722 604 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1015 1259 1313 1375 1461
Size: 1015 244 54 62 86
Fragments arranged by size:
1015 244 86 62 54
BsrBI CCG'CTC
Cuts at: 0 285 660 1314 1461
Size: 285 375 654 147
Fragments arranged by size:
654 375 285 147
BsrDI GCAATG_nn'
Cuts at: 0 705 1461
Size: 705 756
BsrFI r'CCGG_y
Cuts at: 0 35 167 1222 1461
Size: 35 132 1055 239
Fragments arranged by size:
1055 239 132 35
BssSI C'ACGA_G
Cuts at: 0 1375 1461
Size: 1375 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 1461
Size: 113 1348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1461
Size: 57 1404
Bsu36I CC'TnA_GG
Cuts at: 0 232 1461
Size: 232 1229
BtgI C'CryG_G
Cuts at: 0 603 737 1023 1217 1400 1461
Size: 603 134 286 194 183 61
Fragments arranged by size:
603 286 194 183 134 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 444 1461
Size: 444 1017
BtsI GCAGTG_nn'
Cuts at: 0 94 902 955 1461
Size: 94 808 53 506
Fragments arranged by size:
808 506 94 53
DraIII CAC_nnn'GTG
Cuts at: 0 555 1149 1461
Size: 555 594 312
Fragments arranged by size:
594 555 312
EaeI y'GGCC_r
Cuts at: 0 93 212 1016 1038 1220 1224 1309 1461
Size: 93 119 804 22 182 4 85 152
Fragments arranged by size:
804 182 152 119 93 85 22 4
EagI C'GGCC_G
Cuts at: 0 1038 1309 1461
Size: 1038 271 152
Fragments arranged by size:
1038 271 152
EarI CTCTTCn'nnn_
Cuts at: 0 859 1461
Size: 859 602
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 1461
Size: 514 947
EcoICRI GAG'CTC
Cuts at: 0 315 405 456 924 1461
Size: 315 90 51 468 537
Fragments arranged by size:
537 468 315 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 49 79 448 495 601 655 729
Size: 25 24 30 369 47 106 54 74
Cuts at: 729 940 1186 1461
Size: 211 246 275
Fragments arranged by size:
369 275 246 211 106 74 54 47
30 25 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 1168 1461
Size: 817 351 293
Fragments arranged by size:
817 351 293
EcoO109I rG'GnC_Cy
Cuts at: 0 246 1133 1461
Size: 246 887 328
Fragments arranged by size:
887 328 246
FseI GG_CCGG'CC
Cuts at: 0 1226 1461
Size: 1226 235
HaeII r_GCGC'y
Cuts at: 0 993 1086 1461
Size: 993 93 375
Fragments arranged by size:
993 375 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 579 611 1461
Size: 579 32 850
Fragments arranged by size:
850 579 32
KasI G'GCGC_C
Cuts at: 0 1082 1461
Size: 1082 379
KpnI G_GTAC'C
Cuts at: 0 1210 1461
Size: 1210 251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 872 1461
Size: 482 390 589
Fragments arranged by size:
589 482 390
MscI TGG'CCA
Cuts at: 0 95 214 1018 1461
Size: 95 119 804 443
Fragments arranged by size:
804 443 119 95
MslI CAynn'nnrTG
Cuts at: 0 140 602 943 1461
Size: 140 462 341 518
Fragments arranged by size:
518 462 341 140
MspA1I CmG'CkG
Cuts at: 0 515 575 633 969 1025 1421 1461
Size: 515 60 58 336 56 396 40
Fragments arranged by size:
515 396 336 60 58 56 40
NaeI GCC'GGC
Cuts at: 0 169 1224 1461
Size: 169 1055 237
Fragments arranged by size:
1055 237 169
NarI GG'CG_CC
Cuts at: 0 1083 1461
Size: 1083 378
NcoI C'CATG_G
Cuts at: 0 737 1400 1461
Size: 737 663 61
Fragments arranged by size:
737 663 61
NgoMIV G'CCGG_C
Cuts at: 0 167 1222 1461
Size: 167 1055 239
Fragments arranged by size:
1055 239 167
NotI GC'GGCC_GC
Cuts at: 0 1038 1309 1461
Size: 1038 271 152
Fragments arranged by size:
1038 271 152
NspI r_CATG'y
Cuts at: 0 630 1461
Size: 630 831
PasI CC'CwG_GG
Cuts at: 0 241 645 1461
Size: 241 404 816
Fragments arranged by size:
816 404 241
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 738 1461
Size: 555 183 723
Fragments arranged by size:
723 555 183
PfoI T'CCnGG_A
Cuts at: 0 536 917 1358 1461
Size: 536 381 441 103
Fragments arranged by size:
536 441 381 103
PpuMI rG'GwC_Cy
Cuts at: 0 246 1133 1461
Size: 246 887 328
Fragments arranged by size:
887 328 246
PstI C_TGCA'G
Cuts at: 0 89 1461
Size: 89 1372
PvuII CAG'CTG
Cuts at: 0 515 633 1461
Size: 515 118 828
Fragments arranged by size:
828 515 118
SacI G_AGCT'C
Cuts at: 0 317 407 458 926 1461
Size: 317 90 51 468 535
Fragments arranged by size:
535 468 317 90 51
SacII CC_GC'GG
Cuts at: 0 1026 1461
Size: 1026 435
SanDI GG'GwC_CC
Cuts at: 0 246 1461
Size: 246 1215
SapI GCTCTTCn'nnn_
Cuts at: 0 859 1461
Size: 859 602
SexAI A'CCwGG_T
Cuts at: 0 551 1461
Size: 551 910
SfcI C'TryA_G
Cuts at: 0 85 384 999 1461
Size: 85 299 615 462
Fragments arranged by size:
615 462 299 85
SfoI GGC'GCC
Cuts at: 0 1084 1461
Size: 1084 377
SmaI CCC'GGG
Cuts at: 0 655 1204 1461
Size: 655 549 257
Fragments arranged by size:
655 549 257
SmlI C'TyrA_G
Cuts at: 0 886 1461
Size: 886 575
StuI AGG'CCT
Cuts at: 0 812 1461
Size: 812 649
StyI C'CwwG_G
Cuts at: 0 257 737 929 1400 1461
Size: 257 480 192 471 61
Fragments arranged by size:
480 471 257 192 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 507 926 1461
Size: 507 419 535
Fragments arranged by size:
535 507 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1461
Size: 207 1254
XmaI C'CCGG_G
Cuts at: 0 653 1202 1461
Size: 653 549 259
Fragments arranged by size:
653 549 259
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PflMI PfoI
PpuMI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaXI BsiWI BspHI BsrGI BssHII BstBI BstEII
BstYI BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI MfeI MluI
NdeI NheI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI