Restriction map for
pFN21AE4113
(Linear) (Six-base) MAPSORT of: KIEE4113.seq Check: 8660 from: 1 to: 1455
KIEE4113.orf
With 182 enzymes: SgfI *
March 22, 2012 17:23 ..
Acc65I G'GTAC_C
Cuts at: 0 1055 1455
Size: 1055 400
AfeI AGC'GCT
Cuts at: 0 616 1455
Size: 616 839
AflIII A'CryG_T
Cuts at: 0 428 1455
Size: 428 1027
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1247 1279 1455
Size: 1247 32 176
Fragments arranged by size:
1247 176 32
AlwNI CAG_nnn'CTG
Cuts at: 0 693 792 1455
Size: 693 99 663
Fragments arranged by size:
693 663 99
ApaI G_GGCC'C
Cuts at: 0 272 1455
Size: 272 1183
ApaLI G'TGCA_C
Cuts at: 0 253 1455
Size: 253 1202
ApoI r'AATT_y
Cuts at: 0 377 851 1455
Size: 377 474 604
Fragments arranged by size:
604 474 377
AvaI C'yCGr_G
Cuts at: 0 273 601 710 1180 1455
Size: 273 328 109 470 275
Fragments arranged by size:
470 328 275 273 109
BamHI G'GATC_C
Cuts at: 0 1225 1455
Size: 1225 230
BanI G'GyrC_C
Cuts at: 0 64 291 1010 1055 1455
Size: 64 227 719 45 400
Fragments arranged by size:
719 400 227 64 45
BanII G_rGCy'C
Cuts at: 0 178 242 272 401 602 837 1172 1442
Size: 178 64 30 129 201 235 335 270
Cuts at: 1442 1455
Size: 13
Fragments arranged by size:
335 270 235 201 178 129 64 30
13
BbeI G_GCGC'C
Cuts at: 0 68 1455
Size: 68 1387
BbsI GAAGACnn'nnnn_
Cuts at: 0 324 735 1019 1455
Size: 324 411 284 436
Fragments arranged by size:
436 411 324 284
BglI GCCn_nnn'nGGC
Cuts at: 0 649 1127 1455
Size: 649 478 328
Fragments arranged by size:
649 478 328
Bme1580I G_kGCm'C
Cuts at: 0 257 272 296 1455
Size: 257 15 24 1159
Fragments arranged by size:
1159 257 24 15
BmgBI CAC'GTC
Cuts at: 0 252 393 1455
Size: 252 141 1062
Fragments arranged by size:
1062 252 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 771 888 1421 1455
Size: 771 117 533 34
Fragments arranged by size:
771 533 117 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 715 934 1116 1455
Size: 556 159 219 182 339
Fragments arranged by size:
556 339 219 182 159
Bpu10I CC'TnA_GC
Cuts at: 0 1436 1455
Size: 1436 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1455
Size: 1156 299
BsaI GGTCTCn'nnnn_
Cuts at: 0 347 913 1455
Size: 347 566 542
Fragments arranged by size:
566 542 347
BsaHI Gr'CG_yC
Cuts at: 0 65 1455
Size: 65 1390
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 886 931 1380 1455
Size: 610 276 45 449 75
Fragments arranged by size:
610 449 276 75 45
BseYI C'CCAG_C
Cuts at: 0 103 504 620 1116 1455
Size: 103 401 116 496 339
Fragments arranged by size:
496 401 339 116 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1455
Size: 1375 80
BsiEI CG_ry'CG
Cuts at: 0 38 1455
Size: 38 1417
BsiHKAI G_wGCw'C
Cuts at: 0 242 257 1172 1390 1442 1455
Size: 242 15 915 218 52 13
Fragments arranged by size:
915 242 218 52 15 13
Bsp1286I G_dGCh'C
Cuts at: 0 178 242 257 272 296 401 602 837
Size: 178 64 15 15 24 105 201 235
Cuts at: 837 1172 1390 1442 1455
Size: 335 218 52 13
Fragments arranged by size:
335 235 218 201 178 105 64 52
24 15 15 13
BsrFI r'CCGG_y
Cuts at: 0 61 584 683 1455
Size: 61 523 99 772
Fragments arranged by size:
772 523 99 61
BsrGI T'GTAC_A
Cuts at: 0 431 1455
Size: 431 1024
BstAPI GCAn_nnn'nTGC
Cuts at: 0 792 1455
Size: 792 663
BstYI r'GATC_y
Cuts at: 0 1225 1430 1455
Size: 1225 205 25
Fragments arranged by size:
1225 205 25
BtgI C'CryG_G
Cuts at: 0 32 38 71 1059 1455
Size: 32 6 33 988 396
Fragments arranged by size:
988 396 33 32 6
BtsI GCAGTG_nn'
Cuts at: 0 1094 1363 1455
Size: 1094 269 92
Fragments arranged by size:
1094 269 92
ClaI AT'CG_AT
Cuts at: 0 446 1455
Size: 446 1009
EaeI y'GGCC_r
Cuts at: 0 35 245 470 947 1292 1455
Size: 35 210 225 477 345 163
Fragments arranged by size:
477 345 225 210 163 35
EagI C'GGCC_G
Cuts at: 0 35 1455
Size: 35 1420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 376 787 1455
Size: 22 354 411 668
Fragments arranged by size:
668 411 354 22
EcoICRI GAG'CTC
Cuts at: 0 240 1170 1440 1455
Size: 240 930 270 15
Fragments arranged by size:
930 270 240 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 715 934 1116 1455
Size: 556 159 219 182 339
Fragments arranged by size:
556 339 219 182 159
EcoO109I rG'GnC_Cy
Cuts at: 0 268 269 717 993 1455
Size: 268 1 448 276 462
Fragments arranged by size:
462 448 276 268 1
EcoRI G'AATT_C
Cuts at: 0 851 1455
Size: 851 604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 327 1389 1455
Size: 327 1062 66
Fragments arranged by size:
1062 327 66
HaeII r_GCGC'y
Cuts at: 0 32 68 110 124 618 890 1068 1455
Size: 32 36 42 14 494 272 178 387
Fragments arranged by size:
494 387 272 178 42 36 32 14
HincII GTy'rAC
Cuts at: 0 528 1455
Size: 528 927
KasI G'GCGC_C
Cuts at: 0 64 1455
Size: 64 1391
KpnI G_GTAC'C
Cuts at: 0 1059 1455
Size: 1059 396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1455
Size: 880 575
MscI TGG'CCA
Cuts at: 0 472 1455
Size: 472 983
MspA1I CmG'CkG
Cuts at: 0 34 40 1061 1455
Size: 34 6 1021 394
Fragments arranged by size:
1021 394 34 6
NaeI GCC'GGC
Cuts at: 0 63 586 1455
Size: 63 523 869
Fragments arranged by size:
869 523 63
NarI GG'CG_CC
Cuts at: 0 65 1455
Size: 65 1390
NgoMIV G'CCGG_C
Cuts at: 0 61 584 1455
Size: 61 523 871
Fragments arranged by size:
871 523 61
NotI GC'GGCC_GC
Cuts at: 0 35 1455
Size: 35 1420
NspI r_CATG'y
Cuts at: 0 432 823 1455
Size: 432 391 632
Fragments arranged by size:
632 432 391
PciI A'CATG_T
Cuts at: 0 428 1455
Size: 428 1027
PfoI T'CCnGG_A
Cuts at: 0 1304 1455
Size: 1304 151
PshAI GACnn'nnGTC
Cuts at: 0 1414 1455
Size: 1414 41
PspOMI G'GGCC_C
Cuts at: 0 268 1455
Size: 268 1187
RsrII CG'GwC_CG
Cuts at: 0 659 1455
Size: 659 796
SacI G_AGCT'C
Cuts at: 0 242 1172 1442 1455
Size: 242 930 270 13
Fragments arranged by size:
930 270 242 13
SacII CC_GC'GG
Cuts at: 0 35 41 1062 1455
Size: 35 6 1021 393
Fragments arranged by size:
1021 393 35 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 649 1455
Size: 649 806
SfoI GGC'GCC
Cuts at: 0 66 1455
Size: 66 1389
SgrAI Cr'CCGG_yG
Cuts at: 0 61 1455
Size: 61 1394
SmaI CCC'GGG
Cuts at: 0 275 1455
Size: 275 1180
SmlI C'TyrA_G
Cuts at: 0 1171 1455
Size: 1171 284
TatI w'GTAC_w
Cuts at: 0 425 431 494 1151 1455
Size: 425 6 63 657 304
Fragments arranged by size:
657 425 304 63 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1455
Size: 1342 32 81
Fragments arranged by size:
1342 81 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 735 1302 1455
Size: 735 567 153
Fragments arranged by size:
735 567 153
XmaI C'CCGG_G
Cuts at: 0 273 1455
Size: 273 1182
Enzymes that do cut:
Acc65I AfeI AflIII AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BsrGI BstAPI
BstYI BtgI BtsI ClaI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII HincII KasI KpnI
MmeI MscI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PfoI PshAI PspOMI RsrII SacI SacII SfiI
SfoI SgrAI SmaI SmlI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EarI EcoNI EcoRV FseI FspI FspAI Hin4I HindIII
HpaI MfeI MluI MslI NcoI NdeI NheI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI