Restriction map for
pFN21AE4108
(Linear) (Six-base) MAPSORT of: KIEE4108.seq Check: 8962 from: 1 to: 1434
KIEE4108.orf
With 182 enzymes: SgfI *
March 22, 2012 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 702 1434
Size: 702 732
AatII G_ACGT'C
Cuts at: 0 986 1434
Size: 986 448
Acc65I G'GTAC_C
Cuts at: 0 56 1434
Size: 56 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1434
Size: 1111 323
AfeI AGC'GCT
Cuts at: 0 211 1434
Size: 211 1223
AflIII A'CryG_T
Cuts at: 0 661 1007 1070 1434
Size: 661 346 63 364
Fragments arranged by size:
661 364 346 63
AleI CACnn'nnGTG
Cuts at: 0 664 994 1141 1434
Size: 664 330 147 293
Fragments arranged by size:
664 330 293 147
AlwNI CAG_nnn'CTG
Cuts at: 0 516 1279 1389 1434
Size: 516 763 110 45
Fragments arranged by size:
763 516 110 45
ApaI G_GGCC'C
Cuts at: 0 504 949 1434
Size: 504 445 485
Fragments arranged by size:
504 485 445
ApaLI G'TGCA_C
Cuts at: 0 401 1197 1434
Size: 401 796 237
Fragments arranged by size:
796 401 237
AvaI C'yCGr_G
Cuts at: 0 751 1036 1399 1434
Size: 751 285 363 35
Fragments arranged by size:
751 363 285 35
BanI G'GyrC_C
Cuts at: 0 56 470 1434
Size: 56 414 964
Fragments arranged by size:
964 414 56
BanII G_rGCy'C
Cuts at: 0 180 252 504 689 758 897 949 1434
Size: 180 72 252 185 69 139 52 485
Fragments arranged by size:
485 252 185 180 139 72 69 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 978 1434
Size: 978 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 438 472 1434
Size: 438 34 962
Fragments arranged by size:
962 438 34
BclI T'GATC_A
Cuts at: 0 479 1434
Size: 479 955
BglI GCCn_nnn'nGGC
Cuts at: 0 499 1434
Size: 499 935
BlpI GC'TnA_GC
Cuts at: 0 601 1057 1434
Size: 601 456 377
Fragments arranged by size:
601 456 377
Bme1580I G_kGCm'C
Cuts at: 0 143 228 405 504 744 949 1201 1398
Size: 143 85 177 99 240 205 252 197
Cuts at: 1398 1434
Size: 36
Fragments arranged by size:
252 240 205 197 177 143 99 85
36
BmgBI CAC'GTC
Cuts at: 0 78 348 1434
Size: 78 270 1086
Fragments arranged by size:
1086 270 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1320 1434
Size: 1320 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 631 1434
Size: 85 546 803
Fragments arranged by size:
803 546 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1434
Size: 1207 227
BsaAI yAC'GTr
Cuts at: 0 406 662 996 1338 1434
Size: 406 256 334 342 96
Fragments arranged by size:
406 342 334 256 96
BsaHI Gr'CG_yC
Cuts at: 0 646 983 1434
Size: 646 337 451
Fragments arranged by size:
646 451 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 1434
Size: 604 30 800
Fragments arranged by size:
800 604 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 940 1282 1387 1434
Size: 160 780 342 105 47
Fragments arranged by size:
780 342 160 105 47
BseYI C'CCAG_C
Cuts at: 0 951 1434
Size: 951 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1434
Size: 1372 62
BsiEI CG_ry'CG
Cuts at: 0 1107 1434
Size: 1107 327
BsiHKAI G_wGCw'C
Cuts at: 0 405 758 1001 1201 1434
Size: 405 353 243 200 233
Fragments arranged by size:
405 353 243 233 200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 68 1291 1434
Size: 68 1223 143
Fragments arranged by size:
1223 143 68
Bsp1286I G_dGCh'C
Cuts at: 0 143 180 228 252 405 504 689 744
Size: 143 37 48 24 153 99 185 55
Cuts at: 744 758 897 949 1001 1201 1398 1434
Size: 14 139 52 52 200 197 36
Fragments arranged by size:
200 197 185 153 143 139 99 55
52 52 48 37 36 24 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 258 605 702 1085 1415 1434
Size: 68 190 347 97 383 330 19
Fragments arranged by size:
383 347 330 190 97 68 19
BsrBI CCG'CTC
Cuts at: 0 1239 1434
Size: 1239 195
BsrDI GCAATG_nn'
Cuts at: 0 586 1434
Size: 586 848
BsrGI T'GTAC_A
Cuts at: 0 518 1010 1434
Size: 518 492 424
Fragments arranged by size:
518 492 424
BssSI C'ACGA_G
Cuts at: 0 142 1411 1434
Size: 142 1269 23
Fragments arranged by size:
1269 142 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 409 1389 1434
Size: 409 980 45
Fragments arranged by size:
980 409 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1425 1434
Size: 429 996 9
Fragments arranged by size:
996 429 9
Bsu36I CC'TnA_GG
Cuts at: 0 939 1434
Size: 939 495
BtgI C'CryG_G
Cuts at: 0 488 1434
Size: 488 946
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 370 1434
Size: 370 1064
BtsI GCAGTG_nn'
Cuts at: 0 885 1434
Size: 885 549
DraIII CAC_nnn'GTG
Cuts at: 0 232 409 1434
Size: 232 177 1025
Fragments arranged by size:
1025 232 177
EaeI y'GGCC_r
Cuts at: 0 167 572 731 873 1104 1160 1211 1340
Size: 167 405 159 142 231 56 51 129
Cuts at: 1340 1434
Size: 94
Fragments arranged by size:
405 231 167 159 142 129 94 56
51
EagI C'GGCC_G
Cuts at: 0 1104 1434
Size: 1104 330
EarI CTCTTCn'nnn_
Cuts at: 0 1087 1174 1434
Size: 1087 87 260
Fragments arranged by size:
1087 260 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 1162 1434
Size: 232 930 272
Fragments arranged by size:
930 272 232
EcoICRI GAG'CTC
Cuts at: 0 756 1434
Size: 756 678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 631 1111 1434
Size: 85 546 480 323
Fragments arranged by size:
546 480 323 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 285 712 1434
Size: 285 427 722
Fragments arranged by size:
722 427 285
EcoO109I rG'GnC_Cy
Cuts at: 0 500 501 594 945 946 1404 1434
Size: 500 1 93 351 1 458 30
Fragments arranged by size:
500 458 351 93 30 1 1
HaeII r_GCGC'y
Cuts at: 0 213 570 727 1367 1434
Size: 213 357 157 640 67
Fragments arranged by size:
640 357 213 157 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 1434
Size: 616 32 786
Fragments arranged by size:
786 616 32
KpnI G_GTAC'C
Cuts at: 0 60 1434
Size: 60 1374
MscI TGG'CCA
Cuts at: 0 169 574 1162 1213 1342 1434
Size: 169 405 588 51 129 92
Fragments arranged by size:
588 405 169 129 92 51
MslI CAynn'nnrTG
Cuts at: 0 554 664 994 1141 1434
Size: 554 110 330 147 293
Fragments arranged by size:
554 330 293 147 110
MspA1I CmG'CkG
Cuts at: 0 26 182 318 588 809 1276 1314 1434
Size: 26 156 136 270 221 467 38 120
Fragments arranged by size:
467 270 221 156 136 120 38 26
NotI GC'GGCC_GC
Cuts at: 0 1104 1434
Size: 1104 330
NspI r_CATG'y
Cuts at: 0 429 553 1011 1074 1434
Size: 429 124 458 63 360
Fragments arranged by size:
458 429 360 124 63
PciI A'CATG_T
Cuts at: 0 1007 1070 1434
Size: 1007 63 364
Fragments arranged by size:
1007 364 63
PfoI T'CCnGG_A
Cuts at: 0 710 1434
Size: 710 724
PmlI CAC'GTG
Cuts at: 0 662 996 1338 1434
Size: 662 334 342 96
Fragments arranged by size:
662 342 334 96
PpuMI rG'GwC_Cy
Cuts at: 0 594 1404 1434
Size: 594 810 30
Fragments arranged by size:
810 594 30
PspOMI G'GGCC_C
Cuts at: 0 500 945 1434
Size: 500 445 489
Fragments arranged by size:
500 489 445
PspXI vC'TCGA_Gb
Cuts at: 0 1399 1434
Size: 1399 35
PstI C_TGCA'G
Cuts at: 0 31 286 512 699 844 1434
Size: 31 255 226 187 145 590
Fragments arranged by size:
590 255 226 187 145 31
PvuII CAG'CTG
Cuts at: 0 26 588 809 1434
Size: 26 562 221 625
Fragments arranged by size:
625 562 221 26
SacI G_AGCT'C
Cuts at: 0 758 1434
Size: 758 676
SapI GCTCTTCn'nnn_
Cuts at: 0 1174 1434
Size: 1174 260
SbfI CC_TGCA'GG
Cuts at: 0 286 1434
Size: 286 1148
SfcI C'TryA_G
Cuts at: 0 27 282 508 695 840 1359 1434
Size: 27 255 226 187 145 519 75
Fragments arranged by size:
519 255 226 187 145 75 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 499 1434
Size: 499 935
SmlI C'TyrA_G
Cuts at: 0 1186 1399 1434
Size: 1186 213 35
Fragments arranged by size:
1186 213 35
StuI AGG'CCT
Cuts at: 0 862 1088 1434
Size: 862 226 346
Fragments arranged by size:
862 346 226
StyI C'CwwG_G
Cuts at: 0 1214 1434
Size: 1214 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 388 488 1135 1411 1434
Size: 388 100 647 276 23
Fragments arranged by size:
647 388 276 100 23
TatI w'GTAC_w
Cuts at: 0 119 360 518 1010 1205 1434
Size: 119 241 158 492 195 229
Fragments arranged by size:
492 241 229 195 158 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 604 636 1434
Size: 604 32 798
Fragments arranged by size:
798 604 32
Tth111I GACn'n_nGTC
Cuts at: 0 349 1434
Size: 349 1085
XbaI T'CTAG_A
Cuts at: 0 324 1434
Size: 324 1110
XhoI C'TCGA_G
Cuts at: 0 1399 1434
Size: 1399 35
XmnI GAAnn'nnTTC
Cuts at: 0 373 1434
Size: 373 1061
ZraI GAC'GTC
Cuts at: 0 984 1434
Size: 984 450
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BcgI BclI
BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI
MscI MslI MspA1I NotI NspI PciI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI
SfcI SfiI SmlI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BfrBI BglII BmrI BmtI Bpu10I BsaI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrFI BssHII BstBI
BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PflMI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XcmI XmaI