Restriction map for
pFN21AE4103
(Linear) (Six-base) MAPSORT of: KIEE4103.seq Check: 6535 from: 1 to: 1416
KIEE4103.orf
With 182 enzymes: SgfI *
July 3, 2012 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1101 1293 1416
Size: 1101 192 123
Fragments arranged by size:
1101 192 123
AflIII A'CryG_T
Cuts at: 0 617 1416
Size: 617 799
AleI CACnn'nnGTG
Cuts at: 0 257 1416
Size: 257 1159
AlwNI CAG_nnn'CTG
Cuts at: 0 474 633 1290 1416
Size: 474 159 657 126
Fragments arranged by size:
657 474 159 126
ApoI r'AATT_y
Cuts at: 0 679 988 1198 1228 1416
Size: 679 309 210 30 188
Fragments arranged by size:
679 309 210 188 30
AvaI C'yCGr_G
Cuts at: 0 875 1416
Size: 875 541
BamHI G'GATC_C
Cuts at: 0 121 1416
Size: 121 1295
BanII G_rGCy'C
Cuts at: 0 943 1416
Size: 943 473
BbsI GAAGACnn'nnnn_
Cuts at: 0 985 1299 1416
Size: 985 314 117
Fragments arranged by size:
985 314 117
BbvCI CC'TCA_GC
Cuts at: 0 790 1416
Size: 790 626
BfrBI ATG'CAT
Cuts at: 0 222 1416
Size: 222 1194
BglI GCCn_nnn'nGGC
Cuts at: 0 1406 1416
Size: 1406 10
BglII A'GATC_T
Cuts at: 0 405 1277 1416
Size: 405 872 139
Fragments arranged by size:
872 405 139
BlpI GC'TnA_GC
Cuts at: 0 496 1416
Size: 496 920
BmrI ACTGGGnnnn_n'
Cuts at: 0 937 1416
Size: 937 479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1416
Size: 670 746
Bpu10I CC'TnA_GC
Cuts at: 0 790 1416
Size: 790 626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1021 1416
Size: 544 477 395
Fragments arranged by size:
544 477 395
BsaAI yAC'GTr
Cuts at: 0 771 1416
Size: 771 645
BsaBI GATnn'nnATC
Cuts at: 0 167 1416
Size: 167 1249
BsaWI w'CCGG_w
Cuts at: 0 1271 1416
Size: 1271 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 889 919 1416
Size: 889 30 497
Fragments arranged by size:
889 497 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2 103 328 1416
Size: 2 101 225 1088
Fragments arranged by size:
1088 225 101 2
BseYI C'CCAG_C
Cuts at: 0 626 1416
Size: 626 790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1416
Size: 718 698
BsiHKAI G_wGCw'C
Cuts at: 0 269 1416
Size: 269 1147
Bsp1286I G_dGCh'C
Cuts at: 0 269 943 1416
Size: 269 674 473
Fragments arranged by size:
674 473 269
BsrDI GCAATG_nn'
Cuts at: 0 32 1416
Size: 32 1384
BstAPI GCAn_nnn'nTGC
Cuts at: 0 465 1290 1416
Size: 465 825 126
Fragments arranged by size:
825 465 126
BstYI r'GATC_y
Cuts at: 0 121 405 1277 1416
Size: 121 284 872 139
Fragments arranged by size:
872 284 139 121
BtsI GCAGTG_nn'
Cuts at: 0 45 475 1416
Size: 45 430 941
Fragments arranged by size:
941 430 45
EaeI y'GGCC_r
Cuts at: 0 239 1179 1211 1416
Size: 239 940 32 205
Fragments arranged by size:
940 239 205 32
EarI CTCTTCn'nnn_
Cuts at: 0 428 1055 1416
Size: 428 627 361
Fragments arranged by size:
627 428 361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1101 1293 1416
Size: 670 431 192 123
Fragments arranged by size:
670 431 192 123
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1150 1416
Size: 1150 266
EcoO109I rG'GnC_Cy
Cuts at: 0 705 1351 1416
Size: 705 646 65
Fragments arranged by size:
705 646 65
EcoRV GAT'ATC
Cuts at: 0 591 1416
Size: 591 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 1416
Size: 456 32 928
Fragments arranged by size:
928 456 32
HindIII A'AGCT_T
Cuts at: 0 484 764 1416
Size: 484 280 652
Fragments arranged by size:
652 484 280
MscI TGG'CCA
Cuts at: 0 241 1181 1213 1416
Size: 241 940 32 203
Fragments arranged by size:
940 241 203 32
MslI CAynn'nnrTG
Cuts at: 0 227 257 1416
Size: 227 30 1159
Fragments arranged by size:
1159 227 30
MspA1I CmG'CkG
Cuts at: 0 80 633 1416
Size: 80 553 783
Fragments arranged by size:
783 553 80
NsiI A_TGCA'T
Cuts at: 0 224 1416
Size: 224 1192
PpuMI rG'GwC_Cy
Cuts at: 0 705 1351 1416
Size: 705 646 65
Fragments arranged by size:
705 646 65
PstI C_TGCA'G
Cuts at: 0 72 1416
Size: 72 1344
PvuII CAG'CTG
Cuts at: 0 80 633 1416
Size: 80 553 783
Fragments arranged by size:
783 553 80
SapI GCTCTTCn'nnn_
Cuts at: 0 1055 1416
Size: 1055 361
SfcI C'TryA_G
Cuts at: 0 68 732 806 1416
Size: 68 664 74 610
Fragments arranged by size:
664 610 74 68
SmlI C'TyrA_G
Cuts at: 0 559 1000 1416
Size: 559 441 416
Fragments arranged by size:
559 441 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 1416
Size: 336 1080
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 1416
Size: 311 32 1073
Fragments arranged by size:
1073 311 32
XmnI GAAnn'nnTTC
Cuts at: 0 359 683 1416
Size: 359 324 733
Fragments arranged by size:
733 359 324
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanII
BbsI BbvCI BfrBI BglI BglII BlpI BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BstAPI BstYI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I HindIII MscI
MslI MspA1I NsiI PpuMI PstI PvuII SapI SfcI
SmlI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI Bme1580I
BmgBI BmtI BplI BsaI BsaHI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI