Restriction map for
pFN21AE4102
(Linear) (Six-base) MAPSORT of: KIEE4102.seq Check: 2196 from: 1 to: 1413
KIEE4102.orf
With 182 enzymes: SgfI *
March 22, 2012 17:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1413
Size: 177 1236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 739 1413
Size: 739 674
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1218 1405 1413
Size: 309 909 187 8
Fragments arranged by size:
909 309 187 8
ApoI r'AATT_y
Cuts at: 0 425 549 1413
Size: 425 124 864
Fragments arranged by size:
864 425 124
AvrII C'CTAG_G
Cuts at: 0 1017 1413
Size: 1017 396
BanII G_rGCy'C
Cuts at: 0 449 1413
Size: 449 964
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 1102 1413
Size: 826 276 311
Fragments arranged by size:
826 311 276
BciVI GTATCCnnnnn_n'
Cuts at: 0 135 1413
Size: 135 1278
BglII A'GATC_T
Cuts at: 0 330 338 897 1413
Size: 330 8 559 516
Fragments arranged by size:
559 516 330 8
Bme1580I G_kGCm'C
Cuts at: 0 98 1413
Size: 98 1315
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 1062 1229 1413
Size: 70 992 167 184
Fragments arranged by size:
992 184 167 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 226 1413
Size: 79 147 1187
Fragments arranged by size:
1187 147 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 471 1413
Size: 463 8 942
Fragments arranged by size:
942 463 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 267 830 1413
Size: 49 218 563 583
Fragments arranged by size:
583 563 218 49
BseYI C'CCAG_C
Cuts at: 0 206 1211 1413
Size: 206 1005 202
Fragments arranged by size:
1005 206 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 745 1413
Size: 375 370 668
Fragments arranged by size:
668 375 370
BsmI GAATG_Cn'
Cuts at: 0 285 1413
Size: 285 1128
Bsp1286I G_dGCh'C
Cuts at: 0 98 449 1413
Size: 98 351 964
Fragments arranged by size:
964 351 98
BsrGI T'GTAC_A
Cuts at: 0 346 1413
Size: 346 1067
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1413
Size: 57 1356
BstYI r'GATC_y
Cuts at: 0 135 330 338 897 1413
Size: 135 195 8 559 516
Fragments arranged by size:
559 516 195 135 8
EaeI y'GGCC_r
Cuts at: 0 1041 1413
Size: 1041 372
EarI CTCTTCn'nnn_
Cuts at: 0 322 617 1171 1413
Size: 322 295 554 242
Fragments arranged by size:
554 322 295 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1163 1413
Size: 1163 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 177 226 1413
Size: 79 98 49 1187
Fragments arranged by size:
1187 98 79 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1413
Size: 31 1382
EcoO109I rG'GnC_Cy
Cuts at: 0 1198 1413
Size: 1198 215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 457 1126 1413
Size: 457 669 287
Fragments arranged by size:
669 457 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 882 914 986 1018 1120 1152
Size: 703 32 147 32 72 32 102 32
Cuts at: 1152 1413
Size: 261
Fragments arranged by size:
703 261 147 102 72 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 1160 1413
Size: 1160 253
PfoI T'CCnGG_A
Cuts at: 0 127 1413
Size: 127 1286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 1413
Size: 1020 32 361
Fragments arranged by size:
1020 361 32
PpuMI rG'GwC_Cy
Cuts at: 0 1198 1413
Size: 1198 215
PsiI TTA'TAA
Cuts at: 0 1286 1413
Size: 1286 127
ScaI AGT'ACT
Cuts at: 0 64 1413
Size: 64 1349
SmlI C'TyrA_G
Cuts at: 0 442 450 1413
Size: 442 8 963
Fragments arranged by size:
963 442 8
SspI AAT'ATT
Cuts at: 0 847 1413
Size: 847 566
StyI C'CwwG_G
Cuts at: 0 988 1017 1413
Size: 988 29 396
Fragments arranged by size:
988 396 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1081 1258 1413
Size: 1081 177 155
Fragments arranged by size:
1081 177 155
TatI w'GTAC_w
Cuts at: 0 62 346 1413
Size: 62 284 1067
Fragments arranged by size:
1067 284 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 897 929 1077 1109 1413
Size: 897 32 148 32 304
Fragments arranged by size:
897 304 148 32 32
XbaI T'CTAG_A
Cuts at: 0 138 333 429 894 1413
Size: 138 195 96 465 519
Fragments arranged by size:
519 465 195 138 96
XmnI GAAnn'nnTTC
Cuts at: 0 1368 1413
Size: 1368 45
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AvrII BanII BbsI BciVI
BglII Bme1580I BmrI BpmI BpuEI BseRI BseYI BsgI
BsmI Bsp1286I BsrGI BstXI BstYI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI Hin4I MfeI PfoI PpiI
PpuMI PsiI ScaI SmlI SspI StyI TaqII TatI
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XcmI XhoI XmaI
ZraI