Restriction map for
pFN21AE4094
(Linear) (Six-base) MAPSORT of: KIEE4094.seq Check: 339 from: 1 to: 1320
KIEE4094.orf
With 182 enzymes: SgfI *
July 3, 2012 18:33 ..
AccI GT'mk_AC
Cuts at: 0 742 1320
Size: 742 578
AclI AA'CG_TT
Cuts at: 0 1088 1320
Size: 1088 232
AleI CACnn'nnGTG
Cuts at: 0 622 1311 1320
Size: 622 689 9
Fragments arranged by size:
689 622 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1048 1320
Size: 1048 272
AlwNI CAG_nnn'CTG
Cuts at: 0 36 516 730 976 1320
Size: 36 480 214 246 344
Fragments arranged by size:
480 344 246 214 36
ApaLI G'TGCA_C
Cuts at: 0 1127 1320
Size: 1127 193
ApoI r'AATT_y
Cuts at: 0 173 346 1320
Size: 173 173 974
Fragments arranged by size:
974 173 173
BanI G'GyrC_C
Cuts at: 0 597 624 1320
Size: 597 27 696
Fragments arranged by size:
696 597 27
BanII G_rGCy'C
Cuts at: 0 560 1320
Size: 560 760
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 1320
Size: 327 993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 410 939 973 1320
Size: 376 34 529 34 347
Fragments arranged by size:
529 376 347 34 34
BlpI GC'TnA_GC
Cuts at: 0 1160 1320
Size: 1160 160
Bme1580I G_kGCm'C
Cuts at: 0 1131 1320
Size: 1131 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 1164 1320
Size: 1164 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1239 1320
Size: 538 701 81
Fragments arranged by size:
701 538 81
Bpu10I CC'TnA_GC
Cuts at: 0 704 1320
Size: 704 616
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1097 1127 1320
Size: 1097 30 193
Fragments arranged by size:
1097 193 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1320
Size: 94 1226
BseYI C'CCAG_C
Cuts at: 0 699 1320
Size: 699 621
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1320
Size: 704 616
BsiHKAI G_wGCw'C
Cuts at: 0 1131 1320
Size: 1131 189
Bsp1286I G_dGCh'C
Cuts at: 0 560 1131 1320
Size: 560 571 189
Fragments arranged by size:
571 560 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 690 1228 1320
Size: 690 538 92
Fragments arranged by size:
690 538 92
BsrFI r'CCGG_y
Cuts at: 0 468 1320
Size: 468 852
BstAPI GCAn_nnn'nTGC
Cuts at: 0 928 1320
Size: 928 392
BstBI TT'CG_AA
Cuts at: 0 318 1320
Size: 318 1002
BstYI r'GATC_y
Cuts at: 0 567 759 1320
Size: 567 192 561
Fragments arranged by size:
567 561 192
BtsI GCAGTG_nn'
Cuts at: 0 347 1320
Size: 347 973
ClaI AT'CG_AT
Cuts at: 0 949 1320
Size: 949 371
EaeI y'GGCC_r
Cuts at: 0 1245 1320
Size: 1245 75
EarI CTCTTCn'nnn_
Cuts at: 0 431 1320
Size: 431 889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1239 1320
Size: 538 701 81
Fragments arranged by size:
701 538 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1235 1320
Size: 1235 85
EcoO109I rG'GnC_Cy
Cuts at: 0 118 892 1320
Size: 118 774 428
Fragments arranged by size:
774 428 118
EcoRI G'AATT_C
Cuts at: 0 173 1320
Size: 173 1147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 1320
Size: 319 1001
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 1320
Size: 657 32 631
Fragments arranged by size:
657 631 32
HincII GTy'rAC
Cuts at: 0 219 1320
Size: 219 1101
HindIII A'AGCT_T
Cuts at: 0 67 1320
Size: 67 1253
MscI TGG'CCA
Cuts at: 0 1247 1320
Size: 1247 73
MslI CAynn'nnrTG
Cuts at: 0 622 1018 1030 1311 1320
Size: 622 396 12 281 9
Fragments arranged by size:
622 396 281 12 9
MspA1I CmG'CkG
Cuts at: 0 487 730 928 1320
Size: 487 243 198 392
Fragments arranged by size:
487 392 243 198
NaeI GCC'GGC
Cuts at: 0 470 1320
Size: 470 850
NgoMIV G'CCGG_C
Cuts at: 0 468 1320
Size: 468 852
NspI r_CATG'y
Cuts at: 0 258 1029 1320
Size: 258 771 291
Fragments arranged by size:
771 291 258
PfoI T'CCnGG_A
Cuts at: 0 133 1320
Size: 133 1187
PpuMI rG'GwC_Cy
Cuts at: 0 118 892 1320
Size: 118 774 428
Fragments arranged by size:
774 428 118
PstI C_TGCA'G
Cuts at: 0 500 972 1239 1320
Size: 500 472 267 81
Fragments arranged by size:
500 472 267 81
PvuII CAG'CTG
Cuts at: 0 487 730 928 1320
Size: 487 243 198 392
Fragments arranged by size:
487 392 243 198
SanDI GG'GwC_CC
Cuts at: 0 892 1320
Size: 892 428
ScaI AGT'ACT
Cuts at: 0 94 873 1320
Size: 94 779 447
Fragments arranged by size:
779 447 94
SfcI C'TryA_G
Cuts at: 0 496 743 968 1235 1320
Size: 496 247 225 267 85
Fragments arranged by size:
496 267 247 225 85
SphI G_CATG'C
Cuts at: 0 258 1029 1320
Size: 258 771 291
Fragments arranged by size:
771 291 258
SspI AAT'ATT
Cuts at: 0 141 1320
Size: 141 1179
StyI C'CwwG_G
Cuts at: 0 1101 1320
Size: 1101 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 1114 1320
Size: 675 439 206
Fragments arranged by size:
675 439 206
TatI w'GTAC_w
Cuts at: 0 92 871 1320
Size: 92 779 449
Fragments arranged by size:
779 449 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 1097 1129 1320
Size: 129 32 936 32 191
Fragments arranged by size:
936 191 129 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1004 1320
Size: 1004 316
XbaI T'CTAG_A
Cuts at: 0 762 1320
Size: 762 558
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1044 1141 1320
Size: 1044 97 179
Fragments arranged by size:
1044 179 97
Enzymes that do cut:
AccI AclI AleI AlfI AlwNI ApaLI ApoI BanI
BanII BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BstAPI BstBI BstYI BtsI ClaI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII MscI
MslI MspA1I NaeI NgoMIV NspI PfoI PpuMI PstI
PvuII SanDI ScaI SfcI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI SwaI XhoI XmaI XmnI ZraI