Restriction map for
pFN21AE4092
(Linear) (Six-base) MAPSORT of: KIEE4092.seq Check: 1428 from: 1 to: 1398
KIEE4092.orf
With 182 enzymes: SgfI *
March 22, 2012 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1191 1398
Size: 1191 207
AatII G_ACGT'C
Cuts at: 0 410 1398
Size: 410 988
AccI GT'mk_AC
Cuts at: 0 385 1398
Size: 385 1013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 958 1083 1275 1398
Size: 705 253 125 192 123
Fragments arranged by size:
705 253 192 125 123
AflII C'TTAA_G
Cuts at: 0 400 1398
Size: 400 998
AflIII A'CryG_T
Cuts at: 0 1164 1398
Size: 1164 234
AlwNI CAG_nnn'CTG
Cuts at: 0 12 147 276 474 486 621 1398
Size: 12 135 129 198 12 135 777
Fragments arranged by size:
777 198 135 135 129 12 12
ApaI G_GGCC'C
Cuts at: 0 828 1398
Size: 828 570
ApoI r'AATT_y
Cuts at: 0 1008 1180 1398
Size: 1008 172 218
Fragments arranged by size:
1008 218 172
AvaI C'yCGr_G
Cuts at: 0 172 227 242 302 982 1398
Size: 172 55 15 60 680 416
Fragments arranged by size:
680 416 172 60 55 15
BanI G'GyrC_C
Cuts at: 0 150 395 523 1398
Size: 150 245 128 875
Fragments arranged by size:
875 245 150 128
BanII G_rGCy'C
Cuts at: 0 68 208 227 236 581 828 925 1336
Size: 68 140 19 9 345 247 97 411
Cuts at: 1336 1398
Size: 62
Fragments arranged by size:
411 345 247 140 97 68 62 19
9
BbsI GAAGACnn'nnnn_
Cuts at: 0 949 1398
Size: 949 449
BbvCI CC'TCA_GC
Cuts at: 0 46 117 1398
Size: 46 71 1281
Fragments arranged by size:
1281 71 46
BfrBI ATG'CAT
Cuts at: 0 456 1398
Size: 456 942
BglII A'GATC_T
Cuts at: 0 794 1375 1398
Size: 794 581 23
Fragments arranged by size:
794 581 23
BlpI GC'TnA_GC
Cuts at: 0 202 1398
Size: 202 1196
Bme1580I G_kGCm'C
Cuts at: 0 155 398 828 1398
Size: 155 243 430 570
Fragments arranged by size:
570 430 243 155
BmgBI CAC'GTC
Cuts at: 0 852 1167 1398
Size: 852 315 231
Fragments arranged by size:
852 315 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 763 915 1207 1398
Size: 658 105 152 292 191
Fragments arranged by size:
658 292 191 152 105
Bpu10I CC'TnA_GC
Cuts at: 0 46 117 1398
Size: 46 71 1281
Fragments arranged by size:
1281 71 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 1398
Size: 349 1049
BsaAI yAC'GTr
Cuts at: 0 630 1398
Size: 630 768
BsaHI Gr'CG_yC
Cuts at: 0 407 1253 1398
Size: 407 846 145
Fragments arranged by size:
846 407 145
BsaWI w'CCGG_w
Cuts at: 0 308 443 1398
Size: 308 135 955
Fragments arranged by size:
955 308 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 871 901 1398
Size: 871 30 497
Fragments arranged by size:
871 497 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 679 1398
Size: 394 285 719
Fragments arranged by size:
719 394 285
BseYI C'CCAG_C
Cuts at: 0 85 614 1398
Size: 85 529 784
Fragments arranged by size:
784 529 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1398
Size: 460 938
BsiHKAI G_wGCw'C
Cuts at: 0 208 236 581 989 1398
Size: 208 28 345 408 409
Fragments arranged by size:
409 408 345 208 28
Bsp1286I G_dGCh'C
Cuts at: 0 68 155 208 227 236 398 581 828
Size: 68 87 53 19 9 162 183 247
Cuts at: 828 925 989 1336 1398
Size: 97 64 347 62
Fragments arranged by size:
347 247 183 162 97 87 68 64
62 53 19 9
BspEI T'CCGG_A
Cuts at: 0 308 1398
Size: 308 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1191 1398
Size: 1191 207
BsrBI CCG'CTC
Cuts at: 0 642 1052 1398
Size: 642 410 346
Fragments arranged by size:
642 410 346
BsrFI r'CCGG_y
Cuts at: 0 285 1398
Size: 285 1113
BsrGI T'GTAC_A
Cuts at: 0 1274 1398
Size: 1274 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 1398
Size: 150 1248
BstEII G'GTnAC_C
Cuts at: 0 30 1398
Size: 30 1368
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1203 1398
Size: 741 462 195
Fragments arranged by size:
741 462 195
BstYI r'GATC_y
Cuts at: 0 794 1375 1398
Size: 794 581 23
Fragments arranged by size:
794 581 23
Bsu36I CC'TnA_GG
Cuts at: 0 662 1398
Size: 662 736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 405 1398
Size: 405 993
DraIII CAC_nnn'GTG
Cuts at: 0 150 1398
Size: 150 1248
EaeI y'GGCC_r
Cuts at: 0 341 701 896 965 1193 1398
Size: 341 360 195 69 228 205
Fragments arranged by size:
360 341 228 205 195 69
EarI CTCTTCn'nnn_
Cuts at: 0 220 442 1398
Size: 220 222 956
Fragments arranged by size:
956 222 220
EcoICRI GAG'CTC
Cuts at: 0 206 234 579 1398
Size: 206 28 345 819
Fragments arranged by size:
819 345 206 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 705 763 915 958 1083 1207 1275
Size: 658 47 58 152 43 125 124 68
Cuts at: 1275 1398
Size: 123
Fragments arranged by size:
658 152 125 124 123 68 58 47
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 363 1132 1398
Size: 363 769 266
Fragments arranged by size:
769 363 266
EcoO109I rG'GnC_Cy
Cuts at: 0 693 824 1398
Size: 693 131 574
Fragments arranged by size:
693 574 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 1398
Size: 735 663
HaeII r_GCGC'y
Cuts at: 0 112 1398
Size: 112 1286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 441 473 1398
Size: 441 32 925
Fragments arranged by size:
925 441 32
HincII GTy'rAC
Cuts at: 0 386 1170 1398
Size: 386 784 228
Fragments arranged by size:
784 386 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1398
Size: 47 1351
MscI TGG'CCA
Cuts at: 0 703 898 1195 1398
Size: 703 195 297 203
Fragments arranged by size:
703 297 203 195
MslI CAynn'nnrTG
Cuts at: 0 850 916 1398
Size: 850 66 482
Fragments arranged by size:
850 482 66
MspA1I CmG'CkG
Cuts at: 0 276 336 621 1398
Size: 276 60 285 777
Fragments arranged by size:
777 285 276 60
NsiI A_TGCA'T
Cuts at: 0 458 1398
Size: 458 940
NspI r_CATG'y
Cuts at: 0 804 1398
Size: 804 594
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 486 1398
Size: 147 339 912
Fragments arranged by size:
912 339 147
PfoI T'CCnGG_A
Cuts at: 0 98 374 1184 1398
Size: 98 276 810 214
Fragments arranged by size:
810 276 214 98
PmlI CAC'GTG
Cuts at: 0 630 1398
Size: 630 768
PpuMI rG'GwC_Cy
Cuts at: 0 693 1398
Size: 693 705
PspOMI G'GGCC_C
Cuts at: 0 824 1398
Size: 824 574
PspXI vC'TCGA_Gb
Cuts at: 0 982 1398
Size: 982 416
PstI C_TGCA'G
Cuts at: 0 335 595 1398
Size: 335 260 803
Fragments arranged by size:
803 335 260
PvuII CAG'CTG
Cuts at: 0 276 336 621 1398
Size: 276 60 285 777
Fragments arranged by size:
777 285 276 60
SacI G_AGCT'C
Cuts at: 0 208 236 581 1398
Size: 208 28 345 817
Fragments arranged by size:
817 345 208 28
SalI G'TCGA_C
Cuts at: 0 384 1398
Size: 384 1014
SexAI A'CCwGG_T
Cuts at: 0 146 779 1398
Size: 146 633 619
Fragments arranged by size:
633 619 146
SfcI C'TryA_G
Cuts at: 0 331 466 543 591 1020 1398
Size: 331 135 77 48 429 378
Fragments arranged by size:
429 378 331 135 77 48
SmaI CCC'GGG
Cuts at: 0 229 244 304 1398
Size: 229 15 60 1094
Fragments arranged by size:
1094 229 60 15
SmlI C'TyrA_G
Cuts at: 0 400 982 1398
Size: 400 582 416
Fragments arranged by size:
582 416 400
SphI G_CATG'C
Cuts at: 0 804 1398
Size: 804 594
StuI AGG'CCT
Cuts at: 0 679 1398
Size: 679 719
StyI C'CwwG_G
Cuts at: 0 364 582 1398
Size: 364 218 816
Fragments arranged by size:
816 364 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 511 1279 1398
Size: 367 144 768 119
Fragments arranged by size:
768 367 144 119
TatI w'GTAC_w
Cuts at: 0 1274 1398
Size: 1274 124
Tth111I GACn'n_nGTC
Cuts at: 0 28 1398
Size: 28 1370
XhoI C'TCGA_G
Cuts at: 0 982 1398
Size: 982 416
XmaI C'CCGG_G
Cuts at: 0 227 242 302 1398
Size: 227 15 60 1096
Fragments arranged by size:
1096 227 60 15
XmnI GAAnn'nnTTC
Cuts at: 0 760 1398
Size: 760 638
ZraI GAC'GTC
Cuts at: 0 408 1398
Size: 408 990
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BfrBI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgZI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NsiI NspI PflMI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SalI SexAI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI Tth111I XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BglI BmrI BmtI BplI BpuEI
BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BssHII
BssSI BstBI BstZ17I BtgI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI TstI XbaI
XcmI