Restriction map for
pFN21AE4090
(Linear) (Six-base) MAPSORT of: KIEE4090.seq Check: 871 from: 1 to: 1368
KIEE4090.orf
With 182 enzymes: SgfI *
July 3, 2012 18:33 ..
AatII G_ACGT'C
Cuts at: 0 263 1368
Size: 263 1105
AccI GT'mk_AC
Cuts at: 0 989 1368
Size: 989 379
Acc65I G'GTAC_C
Cuts at: 0 1308 1368
Size: 1308 60
AleI CACnn'nnGTG
Cuts at: 0 1363 1368
Size: 1363 5
AvaI C'yCGr_G
Cuts at: 0 346 406 484 1368
Size: 346 60 78 884
Fragments arranged by size:
884 346 78 60
BanI G'GyrC_C
Cuts at: 0 11 179 339 772 1092 1308 1335 1368
Size: 11 168 160 433 320 216 27 33
Fragments arranged by size:
433 320 216 168 160 33 27 11
BanII G_rGCy'C
Cuts at: 0 415 1126 1368
Size: 415 711 242
Fragments arranged by size:
711 415 242
BbsI GAAGACnn'nnnn_
Cuts at: 0 255 468 846 1368
Size: 255 213 378 522
Fragments arranged by size:
522 378 255 213
Bme1580I G_kGCm'C
Cuts at: 0 16 775 1172 1338 1368
Size: 16 759 397 166 30
Fragments arranged by size:
759 397 166 30 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 778 1368
Size: 778 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1291 1368
Size: 561 730 77
Fragments arranged by size:
730 561 77
Bpu10I CC'TnA_GC
Cuts at: 0 1313 1368
Size: 1313 55
BsaAI yAC'GTr
Cuts at: 0 249 1368
Size: 249 1119
BsaHI Gr'CG_yC
Cuts at: 0 197 260 1368
Size: 197 63 1108
Fragments arranged by size:
1108 197 63
BsaWI w'CCGG_w
Cuts at: 0 220 1368
Size: 220 1148
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 628 658 1368
Size: 25 603 30 710
Fragments arranged by size:
710 603 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1368
Size: 931 437
BseYI C'CCAG_C
Cuts at: 0 90 123 187 291 933 1228 1368
Size: 90 33 64 104 642 295 140
Fragments arranged by size:
642 295 140 104 90 64 33
BsiEI CG_ry'CG
Cuts at: 0 138 177 241 513 1368
Size: 138 39 64 272 855
Fragments arranged by size:
855 272 138 64 39
BsiHKAI G_wGCw'C
Cuts at: 0 50 206 415 434 1368
Size: 50 156 209 19 934
Fragments arranged by size:
934 209 156 50 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 67 468 481 631 1368
Size: 67 401 13 150 737
Fragments arranged by size:
737 401 150 67 13
Bsp1286I G_dGCh'C
Cuts at: 0 16 50 206 415 434 775 1126 1172
Size: 16 34 156 209 19 341 351 46
Cuts at: 1172 1338 1368
Size: 166 30
Fragments arranged by size:
351 341 209 166 156 46 34 30
19 16
BsrBI CCG'CTC
Cuts at: 0 39 255 562 1368
Size: 39 216 307 806
Fragments arranged by size:
806 307 216 39
BsrDI GCAATG_nn'
Cuts at: 0 1136 1368
Size: 1136 232
BsrFI r'CCGG_y
Cuts at: 0 176 182 240 547 1368
Size: 176 6 58 307 821
Fragments arranged by size:
821 307 176 58 6
BssHII G'CGCG_C
Cuts at: 0 82 127 154 424 1368
Size: 82 45 27 270 944
Fragments arranged by size:
944 270 82 45 27
BstEII G'GTnAC_C
Cuts at: 0 1356 1368
Size: 1356 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 223 589 819 1368
Size: 223 366 230 549
Fragments arranged by size:
549 366 230 223
BtgI C'CryG_G
Cuts at: 0 7 132 1368
Size: 7 125 1236
Fragments arranged by size:
1236 125 7
ClaI AT'CG_AT
Cuts at: 0 734 1368
Size: 734 634
EaeI y'GGCC_r
Cuts at: 0 56 135 238 821 1368
Size: 56 79 103 583 547
Fragments arranged by size:
583 547 103 79 56
EagI C'GGCC_G
Cuts at: 0 135 238 1368
Size: 135 103 1130
Fragments arranged by size:
1130 135 103
EarI CTCTTCn'nnn_
Cuts at: 0 266 961 1217 1368
Size: 266 695 256 151
Fragments arranged by size:
695 266 256 151
EcoICRI GAG'CTC
Cuts at: 0 413 1368
Size: 413 955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1291 1368
Size: 561 730 77
Fragments arranged by size:
730 561 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 319 1368
Size: 271 48 1049
Fragments arranged by size:
1049 271 48
EcoO109I rG'GnC_Cy
Cuts at: 0 120 888 1368
Size: 120 768 480
Fragments arranged by size:
768 480 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 1368
Size: 723 645
HaeII r_GCGC'y
Cuts at: 0 354 986 1368
Size: 354 632 382
Fragments arranged by size:
632 382 354
HincII GTy'rAC
Cuts at: 0 1005 1368
Size: 1005 363
KpnI G_GTAC'C
Cuts at: 0 1312 1368
Size: 1312 56
MscI TGG'CCA
Cuts at: 0 823 1368
Size: 823 545
MslI CAynn'nnrTG
Cuts at: 0 964 1363 1368
Size: 964 399 5
Fragments arranged by size:
964 399 5
MspA1I CmG'CkG
Cuts at: 0 9 134 384 567 1368
Size: 9 125 250 183 801
Fragments arranged by size:
801 250 183 125 9
NaeI GCC'GGC
Cuts at: 0 242 549 1368
Size: 242 307 819
Fragments arranged by size:
819 307 242
NgoMIV G'CCGG_C
Cuts at: 0 240 547 1368
Size: 240 307 821
Fragments arranged by size:
821 307 240
NotI GC'GGCC_GC
Cuts at: 0 135 1368
Size: 135 1233
NspI r_CATG'y
Cuts at: 0 1254 1368
Size: 1254 114
PasI CC'CwG_GG
Cuts at: 0 1342 1368
Size: 1342 26
PfoI T'CCnGG_A
Cuts at: 0 101 266 1368
Size: 101 165 1102
Fragments arranged by size:
1102 165 101
PmlI CAC'GTG
Cuts at: 0 249 1368
Size: 249 1119
PpuMI rG'GwC_Cy
Cuts at: 0 120 1368
Size: 120 1248
PstI C_TGCA'G
Cuts at: 0 540 1368
Size: 540 828
PvuII CAG'CTG
Cuts at: 0 384 1368
Size: 384 984
SacI G_AGCT'C
Cuts at: 0 415 1368
Size: 415 953
SacII CC_GC'GG
Cuts at: 0 10 135 1368
Size: 10 125 1233
Fragments arranged by size:
1233 125 10
SanDI GG'GwC_CC
Cuts at: 0 120 1368
Size: 120 1248
SexAI A'CCwGG_T
Cuts at: 0 1352 1368
Size: 1352 16
SfcI C'TryA_G
Cuts at: 0 536 1070 1368
Size: 536 534 298
Fragments arranged by size:
536 534 298
SmlI C'TyrA_G
Cuts at: 0 484 1368
Size: 484 884
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1096 1368
Size: 1096 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 628 660 809 841 1368
Size: 27 601 32 149 32 527
Fragments arranged by size:
601 527 149 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 930 1290 1328 1368
Size: 930 360 38 40
Fragments arranged by size:
930 360 40 38
XhoI C'TCGA_G
Cuts at: 0 484 1368
Size: 484 884
ZraI GAC'GTC
Cuts at: 0 261 1368
Size: 261 1107
Enzymes that do cut:
AatII AccI Acc65I AleI AvaI BanI BanII BbsI
Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI
BsrFI BssHII BstEII BstXI BtgI ClaI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII HincII
KpnI MscI MslI MspA1I NaeI NgoMIV NotI NspI
PasI PfoI PmlI PpuMI PstI PvuII SacI SacII
SanDI SexAI SfcI SmlI TaqII TstI XcmI XhoI
ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaBI BsgI BsiWI BsmI BspEI BspHI
BspMI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
MfeI MluI MmeI NarI NcoI NdeI NheI NruI
NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XmaI XmnI