Restriction map for
pFN21AE4089
(Linear) (Six-base) MAPSORT of: KIEE4089.seq Check: 1216 from: 1 to: 1359
KIEE4089.orf
With 182 enzymes: SgfI *
July 3, 2012 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 107 1359
Size: 107 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1359
Size: 1028 331
AflIII A'CryG_T
Cuts at: 0 672 1359
Size: 672 687
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 1359
Size: 1239 120
ApaI G_GGCC'C
Cuts at: 0 141 769 924 1095 1359
Size: 141 628 155 171 264
Fragments arranged by size:
628 264 171 155 141
AscI GG'CGCG_CC
Cuts at: 0 799 1359
Size: 799 560
AvaI C'yCGr_G
Cuts at: 0 134 359 455 556 830 1088 1359
Size: 134 225 96 101 274 258 271
Fragments arranged by size:
274 271 258 225 134 101 96
BanI G'GyrC_C
Cuts at: 0 119 336 697 1288 1359
Size: 119 217 361 591 71
Fragments arranged by size:
591 361 217 119 71
BanII G_rGCy'C
Cuts at: 0 141 462 769 924 1095 1359
Size: 141 321 307 155 171 264
Fragments arranged by size:
321 307 264 171 155 141
BbeI G_GCGC'C
Cuts at: 0 701 1359
Size: 701 658
BbvCI CC'TCA_GC
Cuts at: 0 929 1359
Size: 929 430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 553 1359
Size: 519 34 806
Fragments arranged by size:
806 519 34
BglI GCCn_nnn'nGGC
Cuts at: 0 289 835 1359
Size: 289 546 524
Fragments arranged by size:
546 524 289
BlpI GC'TnA_GC
Cuts at: 0 404 1359
Size: 404 955
Bme1580I G_kGCm'C
Cuts at: 0 124 141 456 529 769 924 1095 1359
Size: 124 17 315 73 240 155 171 264
Fragments arranged by size:
315 264 240 171 155 124 73 17
BmgBI CAC'GTC
Cuts at: 0 705 1359
Size: 705 654
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 1008 1359
Size: 456 552 351
Fragments arranged by size:
552 456 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 709 832 839 1359
Size: 685 24 123 7 520
Fragments arranged by size:
685 520 123 24 7
Bpu10I CC'TnA_GC
Cuts at: 0 929 1359
Size: 929 430
BsaI GGTCTCn'nnnn_
Cuts at: 0 4 164 1132 1359
Size: 4 160 968 227
Fragments arranged by size:
968 227 160 4
BsaAI yAC'GTr
Cuts at: 0 645 1359
Size: 645 714
BsaHI Gr'CG_yC
Cuts at: 0 634 698 1359
Size: 634 64 661
Fragments arranged by size:
661 634 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 587 617 1359
Size: 587 30 742
Fragments arranged by size:
742 587 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 535 1306 1359
Size: 18 517 771 53
Fragments arranged by size:
771 517 53 18
BseYI C'CCAG_C
Cuts at: 0 98 174 754 1359
Size: 98 76 580 605
Fragments arranged by size:
605 580 98 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 625 847 1359
Size: 531 94 222 512
Fragments arranged by size:
531 512 222 94
BsiEI CG_ry'CG
Cuts at: 0 839 1359
Size: 839 520
BsiHKAI G_wGCw'C
Cuts at: 0 381 1160 1181 1359
Size: 381 779 21 178
Fragments arranged by size:
779 381 178 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 625 1359
Size: 625 734
Bsp1286I G_dGCh'C
Cuts at: 0 124 141 381 456 462 529 769 924
Size: 124 17 240 75 6 67 240 155
Cuts at: 924 1095 1160 1181 1359
Size: 171 65 21 178
Fragments arranged by size:
240 240 178 171 155 124 75 67
65 21 17 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 204 215 1337 1359
Size: 107 97 11 1122 22
Fragments arranged by size:
1122 107 97 22 11
BsrBI CCG'CTC
Cuts at: 0 1314 1359
Size: 1314 45
BsrFI r'CCGG_y
Cuts at: 0 80 803 1280 1359
Size: 80 723 477 79
Fragments arranged by size:
723 477 80 79
BssHII G'CGCG_C
Cuts at: 0 655 799 1257 1359
Size: 655 144 458 102
Fragments arranged by size:
655 458 144 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1359
Size: 1050 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1169 1359
Size: 1169 190
Bsu36I CC'TnA_GG
Cuts at: 0 1318 1359
Size: 1318 41
BtgI C'CryG_G
Cuts at: 0 959 1359
Size: 959 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 333 1359
Size: 333 1026
BtsI GCAGTG_nn'
Cuts at: 0 109 574 1024 1071 1303 1359
Size: 109 465 450 47 232 56
Fragments arranged by size:
465 450 232 109 56 47
DraIII CAC_nnn'GTG
Cuts at: 0 749 1359
Size: 749 610
DrdI GACnn_nn'nnGTC
Cuts at: 0 178 1359
Size: 178 1181
EaeI y'GGCC_r
Cuts at: 0 836 896 1359
Size: 836 60 463
Fragments arranged by size:
836 463 60
EagI C'GGCC_G
Cuts at: 0 836 1359
Size: 836 523
EarI CTCTTCn'nnn_
Cuts at: 0 199 233 1359
Size: 199 34 1126
Fragments arranged by size:
1126 199 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1359
Size: 37 1322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 709 832 839 1028 1359
Size: 685 24 123 7 189 331
Fragments arranged by size:
685 331 189 123 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 211 1359
Size: 55 156 1148
Fragments arranged by size:
1148 156 55
EcoO109I rG'GnC_Cy
Cuts at: 0 765 891 920 933 1092 1359
Size: 765 126 29 13 159 267
Fragments arranged by size:
765 267 159 126 29 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1123 1359
Size: 1123 236
FspI TGC'GCA
Cuts at: 0 715 1359
Size: 715 644
HaeII r_GCGC'y
Cuts at: 0 701 852 1359
Size: 701 151 507
Fragments arranged by size:
701 507 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 406 1359
Size: 374 32 953
Fragments arranged by size:
953 374 32
KasI G'GCGC_C
Cuts at: 0 697 1359
Size: 697 662
MslI CAynn'nnrTG
Cuts at: 0 256 958 1359
Size: 256 702 401
Fragments arranged by size:
702 401 256
MspA1I CmG'CkG
Cuts at: 0 178 279 861 1230 1277 1359
Size: 178 101 582 369 47 82
Fragments arranged by size:
582 369 178 101 82 47
NaeI GCC'GGC
Cuts at: 0 805 1282 1359
Size: 805 477 77
Fragments arranged by size:
805 477 77
NarI GG'CG_CC
Cuts at: 0 698 1359
Size: 698 661
NcoI C'CATG_G
Cuts at: 0 959 1359
Size: 959 400
NgoMIV G'CCGG_C
Cuts at: 0 803 1280 1359
Size: 803 477 79
Fragments arranged by size:
803 477 79
NspI r_CATG'y
Cuts at: 0 676 1359
Size: 676 683
PciI A'CATG_T
Cuts at: 0 672 1359
Size: 672 687
PfoI T'CCnGG_A
Cuts at: 0 809 983 1359
Size: 809 174 376
Fragments arranged by size:
809 376 174
PmlI CAC'GTG
Cuts at: 0 645 1359
Size: 645 714
PpuMI rG'GwC_Cy
Cuts at: 0 933 1359
Size: 933 426
PspOMI G'GGCC_C
Cuts at: 0 137 765 920 1091 1359
Size: 137 628 155 171 268
Fragments arranged by size:
628 268 171 155 137
PstI C_TGCA'G
Cuts at: 0 56 212 781 977 1235 1359
Size: 56 156 569 196 258 124
Fragments arranged by size:
569 258 196 156 124 56
PvuII CAG'CTG
Cuts at: 0 178 279 1230 1359
Size: 178 101 951 129
Fragments arranged by size:
951 178 129 101
SfcI C'TryA_G
Cuts at: 0 52 208 353 777 973 1191 1231 1359
Size: 52 156 145 424 196 218 40 128
Fragments arranged by size:
424 218 196 156 145 128 52 40
SfoI GGC'GCC
Cuts at: 0 699 1359
Size: 699 660
SmaI CCC'GGG
Cuts at: 0 136 832 1359
Size: 136 696 527
Fragments arranged by size:
696 527 136
SrfI GCCC'GGGC
Cuts at: 0 832 1359
Size: 832 527
StuI AGG'CCT
Cuts at: 0 153 448 1359
Size: 153 295 911
Fragments arranged by size:
911 295 153
StyI C'CwwG_G
Cuts at: 0 500 959 1222 1359
Size: 500 459 263 137
Fragments arranged by size:
500 459 263 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 1134 1359
Size: 297 837 225
Fragments arranged by size:
837 297 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 1359
Size: 587 32 740
Fragments arranged by size:
740 587 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 582 1359
Size: 255 327 777
Fragments arranged by size:
777 327 255
XmaI C'CCGG_G
Cuts at: 0 134 830 1359
Size: 134 696 529
Fragments arranged by size:
696 529 134
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AscI AvaI BanI
BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmlI
PpuMI PspOMI PstI PvuII SfcI SfoI SmaI SrfI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglII BmtI
BplI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraI EcoICRI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI