Restriction map for
pFN21AE4085
(Linear) (Six-base) MAPSORT of: KIEE4085.seq Check: 155 from: 1 to: 1338
KIEE4085.orf
With 182 enzymes: SgfI *
July 3, 2012 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 174 474 578 717 728 1338
Size: 174 300 104 139 11 610
Fragments arranged by size:
610 300 174 139 104 11
AccI GT'mk_AC
Cuts at: 0 617 1338
Size: 617 721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 1338
Size: 430 908
AlwNI CAG_nnn'CTG
Cuts at: 0 12 363 1137 1338
Size: 12 351 774 201
Fragments arranged by size:
774 351 201 12
ApaI G_GGCC'C
Cuts at: 0 324 545 641 1113 1269 1276 1292 1338
Size: 324 221 96 472 156 7 16 46
Fragments arranged by size:
472 324 221 156 96 46 16 7
ApaLI G'TGCA_C
Cuts at: 0 709 994 1338
Size: 709 285 344
Fragments arranged by size:
709 344 285
AvaI C'yCGr_G
Cuts at: 0 37 757 1262 1268 1338
Size: 37 720 505 6 70
Fragments arranged by size:
720 505 70 37 6
BanI G'GyrC_C
Cuts at: 0 349 593 838 1156 1170 1338
Size: 349 244 245 318 14 168
Fragments arranged by size:
349 318 245 244 168 14
BanII G_rGCy'C
Cuts at: 0 83 324 479 545 641 942 1113 1205
Size: 83 241 155 66 96 301 171 92
Cuts at: 1205 1256 1269 1276 1292 1338
Size: 51 13 7 16 46
Fragments arranged by size:
301 241 171 155 96 92 83 66
51 46 16 13 7
BbeI G_GCGC'C
Cuts at: 0 1160 1338
Size: 1160 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 927 1338
Size: 893 34 411
Fragments arranged by size:
893 411 34
BglI GCCn_nnn'nGGC
Cuts at: 0 272 1048 1296 1338
Size: 272 776 248 42
Fragments arranged by size:
776 272 248 42
BglII A'GATC_T
Cuts at: 0 605 1338
Size: 605 733
BlpI GC'TnA_GC
Cuts at: 0 82 138 1338
Size: 82 56 1200
Fragments arranged by size:
1200 82 56
Bme1580I G_kGCm'C
Cuts at: 0 324 545 641 713 892 998 1113 1269
Size: 324 221 96 72 179 106 115 156
Cuts at: 1269 1276 1292 1338
Size: 7 16 46
Fragments arranged by size:
324 221 179 156 115 106 96 72
46 16 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 943 1338
Size: 228 715 395
Fragments arranged by size:
715 395 228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 1338
Size: 228 1110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 398 946 1338
Size: 39 359 548 392
Fragments arranged by size:
548 392 359 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1338
Size: 475 863
BsaI GGTCTCn'nnnn_
Cuts at: 0 374 391 1338
Size: 374 17 947
Fragments arranged by size:
947 374 17
BsaAI yAC'GTr
Cuts at: 0 708 1338
Size: 708 630
BsaHI Gr'CG_yC
Cuts at: 0 665 734 1157 1338
Size: 665 69 423 181
Fragments arranged by size:
665 423 181 69
BsaWI w'CCGG_w
Cuts at: 0 513 1338
Size: 513 825
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 1338
Size: 181 30 1127
Fragments arranged by size:
1127 181 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 988 1213 1338
Size: 595 393 225 125
Fragments arranged by size:
595 393 225 125
BseYI C'CCAG_C
Cuts at: 0 885 1043 1052 1130 1338
Size: 885 158 9 78 208
Fragments arranged by size:
885 208 158 78 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 400 418 622 1207 1338
Size: 49 351 18 204 585 131
Fragments arranged by size:
585 351 204 131 49 18
BsiEI CG_ry'CG
Cuts at: 0 114 514 1338
Size: 114 400 824
Fragments arranged by size:
824 400 114
BsiHKAI G_wGCw'C
Cuts at: 0 83 491 713 998 1338
Size: 83 408 222 285 340
Fragments arranged by size:
408 340 285 222 83
Bsp1286I G_dGCh'C
Cuts at: 0 83 324 479 491 545 641 713 892
Size: 83 241 155 12 54 96 72 179
Cuts at: 892 942 998 1113 1205 1256 1269 1276 1292
Size: 50 56 115 92 51 13 7 16
Cuts at: 1292 1338
Size: 46
Fragments arranged by size:
241 179 155 115 96 92 83 72
56 54 51 50 46 16 13 12
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 174 422 474 578 717 728 761 1031
Size: 174 248 52 104 139 11 33 270
Cuts at: 1031 1338
Size: 307
Fragments arranged by size:
307 270 248 174 139 104 52 33
11
BsrBI CCG'CTC
Cuts at: 0 785 1017 1338
Size: 785 232 321
Fragments arranged by size:
785 321 232
BssHII G'CGCG_C
Cuts at: 0 291 1338
Size: 291 1047
BssSI C'ACGA_G
Cuts at: 0 436 1338
Size: 436 902
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 1338
Size: 63 1275
BstYI r'GATC_y
Cuts at: 0 605 1338
Size: 605 733
Bsu36I CC'TnA_GG
Cuts at: 0 455 1166 1338
Size: 455 711 172
Fragments arranged by size:
711 455 172
BtgI C'CryG_G
Cuts at: 0 462 545 1213 1233 1248 1338
Size: 462 83 668 20 15 90
Fragments arranged by size:
668 462 90 83 20 15
BtsI GCAGTG_nn'
Cuts at: 0 268 890 1338
Size: 268 622 448
Fragments arranged by size:
622 448 268
DraIII CAC_nnn'GTG
Cuts at: 0 1002 1338
Size: 1002 336
EaeI y'GGCC_r
Cuts at: 0 1087 1338
Size: 1087 251
EarI CTCTTCn'nnn_
Cuts at: 0 781 1338
Size: 781 557
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 1087 1233 1338
Size: 169 918 146 105
Fragments arranged by size:
918 169 146 105
EcoICRI GAG'CTC
Cuts at: 0 81 1338
Size: 81 1257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 398 946 1338
Size: 39 359 548 392
Fragments arranged by size:
548 392 359 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 16 724 832 1338
Size: 16 708 108 506
Fragments arranged by size:
708 506 108 16
EcoO109I rG'GnC_Cy
Cuts at: 0 9 21 320 372 411 541 638 908
Size: 9 12 299 52 39 130 97 270
Cuts at: 908 1020 1075 1109 1272 1273 1288 1289 1298
Size: 112 55 34 163 1 15 1 9
Cuts at: 1298 1338
Size: 40
Fragments arranged by size:
299 270 163 130 112 97 55 52
40 39 34 15 12 9 9 1
1
HaeII r_GCGC'y
Cuts at: 0 886 1160 1338
Size: 886 274 178
Fragments arranged by size:
886 274 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1338
Size: 43 32 1263
Fragments arranged by size:
1263 43 32
KasI G'GCGC_C
Cuts at: 0 1156 1338
Size: 1156 182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 717 992 1338
Size: 95 622 275 346
Fragments arranged by size:
622 346 275 95
MspA1I CmG'CkG
Cuts at: 0 86 966 1029 1047 1056 1137 1215 1235
Size: 86 880 63 18 9 81 78 20
Cuts at: 1235 1338
Size: 103
Fragments arranged by size:
880 103 86 81 78 63 20 18
9
NarI GG'CG_CC
Cuts at: 0 1157 1338
Size: 1157 181
NcoI C'CATG_G
Cuts at: 0 1248 1338
Size: 1248 90
PasI CC'CwG_GG
Cuts at: 0 12 537 625 1338
Size: 12 525 88 713
Fragments arranged by size:
713 525 88 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 12 1137 1338
Size: 12 1125 201
Fragments arranged by size:
1125 201 12
PfoI T'CCnGG_A
Cuts at: 0 923 1322 1338
Size: 923 399 16
Fragments arranged by size:
923 399 16
PmlI CAC'GTG
Cuts at: 0 708 1338
Size: 708 630
PpuMI rG'GwC_Cy
Cuts at: 0 21 411 1020 1075 1338
Size: 21 390 609 55 263
Fragments arranged by size:
609 390 263 55 21
PspOMI G'GGCC_C
Cuts at: 0 320 541 637 1109 1265 1272 1288 1338
Size: 320 221 96 472 156 7 16 50
Fragments arranged by size:
472 320 221 156 96 50 16 7
PstI C_TGCA'G
Cuts at: 0 47 171 485 725 897 971 1028 1338
Size: 47 124 314 240 172 74 57 310
Fragments arranged by size:
314 310 240 172 124 74 57 47
PvuII CAG'CTG
Cuts at: 0 966 1047 1056 1137 1338
Size: 966 81 9 81 201
Fragments arranged by size:
966 201 81 81 9
SacI G_AGCT'C
Cuts at: 0 83 1338
Size: 83 1255
SacII CC_GC'GG
Cuts at: 0 1216 1236 1338
Size: 1216 20 102
Fragments arranged by size:
1216 102 20
SanDI GG'GwC_CC
Cuts at: 0 1075 1338
Size: 1075 263
SbfI CC_TGCA'GG
Cuts at: 0 485 1338
Size: 485 853
ScaI AGT'ACT
Cuts at: 0 848 1338
Size: 848 490
SexAI A'CCwGG_T
Cuts at: 0 23 1338
Size: 23 1315
SfcI C'TryA_G
Cuts at: 0 43 167 481 721 893 967 1024 1338
Size: 43 124 314 240 172 74 57 314
Fragments arranged by size:
314 314 240 172 124 74 57 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1048 1296 1338
Size: 1048 248 42
Fragments arranged by size:
1048 248 42
SfoI GGC'GCC
Cuts at: 0 1158 1338
Size: 1158 180
SmaI CCC'GGG
Cuts at: 0 1270 1338
Size: 1270 68
SmlI C'TyrA_G
Cuts at: 0 490 1338
Size: 490 848
StuI AGG'CCT
Cuts at: 0 496 586 979 1338
Size: 496 90 393 359
Fragments arranged by size:
496 393 359 90
StyI C'CwwG_G
Cuts at: 0 1248 1338
Size: 1248 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 189 1338
Size: 176 13 1149
Fragments arranged by size:
1149 176 13
TatI w'GTAC_w
Cuts at: 0 846 1338
Size: 846 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 1338
Size: 181 32 1125
Fragments arranged by size:
1125 181 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1320 1338
Size: 1320 18
XmaI C'CCGG_G
Cuts at: 0 1268 1338
Size: 1268 70
Enzymes that do cut:
AarI AccI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BcgI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BssHII BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MspA1I NarI NcoI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BmgBI
BmtI Bpu10I BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SapI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI